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냉동보관 후 이식한 지방조직의 생물학적 변화와 생존에 관한 연구
한재정,권용석,이근철,김석권,이장호,허정,김명훈 대한성형외과학회 2009 Archives of Plastic Surgery Vol.36 No.2
Purpose: Adipose tissue injection as a free graft for the correction of soft - tissue deficiency or depression deformity is a widespread procedure in plastic surgery. This study is to analyze the changes and viability of cryopreserved adipose tissue and to find out efficient long - term storage period. Methods: After centrifugation of aspirated abdominal tissues, 10 ㎖ of packed Adipose tissue were freezed at -20℃. For 2, 4, 6, 8 months, each frozen samples were taken and injected into scalp of SCID mice. After 15 weeks, injected Adipose tissue were sampled and analyzed at 2 months interval. We compared and analyzed each group about the weight of the injected fat, histologic impressions, activity of mitochondria, size of a fat cell and rate of survival. Results: Significant weight changes were observed in cryopreservation for 2 months(p<0.05). Histologic changes were observed, independent of the freezing period with H - E stain. Among cryopreservations for 2, 4, 6 months, no significant change were observed. The reduction of mitochondrial enzymatic activity was observed independent of time interval but activity of mitochondrial dehydrogenase was reduced less than 50% in MTT assay. Conclusion: Freezing in -20℃ for 6 months has no adverse effect to Adipose tissue, but fragile adipocytes, damaged cell membrane during harvesting procedure, were disrupted within 1 - 2 month and the maximum volume reduction were followed less than 2 months. These results demonstrate that tissue preparation cells without membrane damage have the greatest viability level and cryopreservation less than 2 months has great volume effect and cryopreservation for 6 months has stable volume effect.
한재정(Jae Jeong Han),황기섭(Gi Seop Hwang),박성민(Seong Min Park),고용권(Yong Kwon Ko) 유공압건설기계학회 2016 유공압건설기계학회 학술대회논문집 Vol.2016 No.11
In general, centrifugal pumps in the underground water by the rotation of the motor of the motor pump may be used in ground water wells. In this paper, by supplying power to the electromagnet of the upper and lower double-acting water pumps and to study the effects of using force and repulsion. Small underground water wells and boreholes (NX standard (76mm)) in diameter 45㎜ water pumps through performance tests for personnel and repulsive forces between the small water pumps that can be used to develop an electromagnet and a permanent magnet for the pressure to 17bar and flow 10.44L/min derived for the performance.
한재정(J. J. Han),박성민(S. M. Park),고용권(Y. K. Ko),권장순(J. S. Kwon) 유공압건설기계학회 2015 유공압건설기계학회 학술대회논문집 Vol.2015 No.10
In general, centrifugal pumps in the underground water by the rotation of the motor of the motor pump may be used in ground water wells. In this paper, by supplying power to the electromagnet of the upper and lower double-acting water pumps and to study the effects of using force and repulsion. Small underground water wells and boreholes (NX standard (76mm)) in diameter 45㎜ water pumps through performance tests for personnel and repulsive forces between the small water pumps that can be used to develop an electromagnet and a permanent magnet for the pressure to 10bar and flow 4L/min derived for the performance.