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RISS 인기검색어
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- 저자 : 한상섭(Sang-Sub Han) (검색결과 30 건)
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한상섭,사동민,이양수,Velmurugan, Natarajan,Han, Sang-Sub,Sa, Dong-Min,Lee, Yang-Soo 한국현미경학회 2007 Applied microscopy Vol.37 No.4
The genus Daldinia is a member of the Xylariaceae that has brown to dark brown and phaseoliform single cell ascospores with a conspicuous full germ slit. The isolate of D. childiae collected from Mt. Deuk-yu in Korea were compared with similar taxa, D. eschscholzii and D. concentrica.. Ascospores were $11{\sim}13{\mu}m{\times}5.5{\sim}6{\mu}m$ in size, light brown to brown, unicellular, ellipsoid-inequilateral, with dull round ends. Ascospore showed very faint ornamentation at ${\times}7.0k$ magnification. It is one of the main morphological characteristic Korean collection of D. childiae under SEM level and a main reason of reinterpretation of D. concentrica in Korea. Using KOH-extractable pigment color of stroma, D. concentrica that also has supported the SEM level investigation. Daldinia concentrica, having those characteristic recorded in Korea, should be renamed as D. childiae. However, the isolate will be the first record as D. childiae, having precise morphological description in Korea.
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Silica Gel 을 담체로한 효소의 고정화 및 충전층 반응기에서의 반응속도론적 해석
한상섭,조찬휘,이한주 ( Sang Sub Han,Chan Hwi Cho,Hanju Lee ) 한국화학공학회 1991 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.29 No.2
This study is for the immobilization of a hydrolytic enzyme, trypsin, within cross-linked silica gels and their kinetic behaviors in a packed bed reactor using a sensible amide substrate, DL-BAPNA In the range of linear velocity of 25 to 200㎝/hr, Michaelis constants(Km`) and maximum reaction rates(Vm`) for immobilized trypsin were less and greater than those for a soluble trypsin, respectively. In comparison with free enzyme, Michaelis constants decreased but maximum reaction rates increased as linear velocity increased. In the case of supported silica-gel with the spherical diameter of 0.6㎜, the internal diffusion resistance was negligible because the effectiveness factor was found to be in the range of 0.9∼1 and the mass transfer coefficient(K_s) was in the range of 8.82×10^(-4) to 1.75×10^(-3)㎝/sec. In addition, Michaelis constant was the function of the amount of immobilized trypsin(immobilization density) and the conversion was found to be maximized at the immobilization density of 10 ㎎ trypsin/g silica gel.
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Consideration of Hypoxylon truncatum, Based on Morphological Characteristics of Korean Collection
한상섭,사동민,이양수,Velmurugan, Natarajan,Han, Sang-Sub,Sa, Dong-Min,Lee, Yang-Soo 한국현미경학회 2007 Applied microscopy Vol.37 No.4
The genus Hypoxylon is a member of the Xylariaceae that has brown to dark brown and phaseoliform single cell ascospores with a conspicuous full germ slit. Hypoxylon truncatum differs from H. annulatum, it has conspicuous perithecial mounds, dark brown stromatal surface, and absence of enriched ostiola disc. The isolate of Hypoxylon, collected from Mt. Deuk-yu in Korea, is compared with similar taxa and described. The isolation of H. truncatum is compared with similar taxa, H. annulatum, with a precise description in the SEM level in Korea.
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임태헌,최용화,이동운,한상섭,차병진,Lim, Tae-Heon,Choi, Yong-Hwa,Lee, Dong-Woon,Han, Sang-Sub,Cha, Byeong-Jin The Korean Society of Pesticide Science 2008 농약과학회지 Vol.12 No.3
복숭아 미이라 과일로부터 복숭아 잿빛무늬병 Monilinia fructicola에 대하여 강한 항균활성을 보이는 방선균 Streptomyces padanus TH04를 분리하였다. TH04 균주의 배양 추출물 1%를 함유한 배지에서의 균사생육 및 포자발아는 시험한 M. fructicola의 strain에 따라 각각 $79.8{\sim}81%$와 $73.9{\sim}75.8%$ 억제되었다. 병원균과 TH04 균주 초기접종 밀도를 0.01%, 0.1% 및 1%로 달리하여 동시배양 한 결과, 항균활성은 선발 방선균의 밀도에 따라 $7.5%{\sim}94%$로 나타났다. 사과(품종; 후지)를 이용한 조추출물의 항균활성은 0.1% 처리구 85.9%, 1%처리구 100%로 나타났다. 항균활성 물질 생산, 안정성 및 제형화에 관한 연구가 이루어질 경우 선발한 Streptomyces padanus TH04는 생물학적 방제제로의 개발 가능성 있을 것으로 생각된다. The Streptomyces padanus isolate TH04, isolated from mummified peaches, showed strong antifungal activity to Monilinia fructicola. The inhibition activity of the isolate TH04 to mycelial growth and spore germination at 1% concentration of sub-antifungal powder made from culture suspension (CS) was ranged from 79.8% to 81.0% and from 73.9% to 75.8% to M. fructicola four strains, respectively. In the test of antifungal activity in mixed culture of the isolate and M. fructicola, inhibition rate was 7.5%, 86.8% and 94.0% in 0.01, 0.1, and 1% concentration of CS containing bacterial cell of the isolate, respectively. On apples (cultivar; Fuji), the control values of the isolate TH04 crude filtrates (0.1 and 1%) were 85.9% and 100%, respectively. The results suggest that the isolate TH04 indicate development possibility as biocontrol agent of brown rot caused by M. fructicola with the study on delivery method and fermentation condition to produce an antifungal compound.
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SSCP기법에 의한 뽕나무오갈병 파이토플라스의 유전적 다형성 분석
한상섭 ( Sang Sub Han ) 한국산림과학회 2013 한국산림과학회지 Vol.102 No.2
Phytoplasmas were detected consistently in 42 mulberry cultivars showing dwarf disease using DNA analysis by amplification with phytoplasma universal primer pairs P1/P7 (about 1.8 kb and R16F2n/R2 (about 1.2 kb). The point mutation from 42 cultivars of mulberry tree was detected by single-strand conformation polymorphism (SSCP) analysis. The SSCP profiles were clearly observed from all of cultivars in 8% polyacrylamide gel, electrophoresizing for and running 8-15 hrs. at 150V, 10oC. The MD and JWB phytoplasma PCR products was mixed and electrophoresis was performed to detect their polymorphism. In this results, the SSCP profiles of all bands of MD and JWB were analyzed on single lane and were distinct in their each of band patterns. The SSCP analysis was possible to detect of 1.8 kb and 1.2 kb nucleotide size and near close band patterns were distinct by mix of two samples. Previously, it was only possible to detect of point mutation under 600 bp nucleotide sequence by SSCP analysis but this modification of SSCP technique was possible to detect clearly SSCP band patterns of about 1.8 kb and 1.2 kb nucleotides.
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