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금속 와이어의 무구속 열간 인발 특성 -스테인레스강 와이어와 구리 와이어-
하보근,김종성,백영남,허유,Ha, Bo-Keun,Kim, Jong-Seong,Paik, Young-Nam,Huh, You 한국섬유공학회 2010 한국섬유공학회지 Vol.47 No.5
Micro-sized metal fibers are suitable for multi-functional and high performance industrial components, but the fiber manufacturing process relies heavily on wire drawing with dies, which can cause die-raw material friction. An alternative for the efficient production of the metal fibers is to use dieless wire drawing technology. This paper reports the characteristic differences of the dieless wire drawing process, which relies on forced necking. The hot working principle was applied to the most frequently used raw materials, stainless steel and copper, while a microwave furnace and susceptor were employed. The experimental trials showed that stainless steel wires could be more advantageous in dieless drawing than copper wires in that the necking of the stainless steel wire took place in a narrower zone than copper. Furthermore, a mathematical model was set up considering volume heating and wire cooling and combining the temperature effect of viscosity in Arrhenius form with the plastic deformation in Bingham form. The model showed good agreement with the experiments when the process was carried out in a steady state at various draw ratios and process speeds. These results suggest that the theoretical model can depict the real process quite well.
하보근,H. Roger Boerma 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.2
Melting curve analysis of fluorescently labeled DNA fragments is used extensively for genotyping single nucleotide polymorphism (SNP). Here, we evaluated a SNP genotyping method by melting curve analysis with the two probe chemistries in a 384-well plate format on a Roche LightCycler 480. The HybProbe chemistry is based on the fluorescence resonance energy transfer (FRET) and the SimpleProbe chemistry uses a terminal self-quenching fluorophore. We evaluated FRET HybProbes and SimpleProbes for two SNP sites closely linked to two quantitative trait loci (QTL) for southern root-knot nematode resistance. These probes were used to genotype the two parents and 94 F₂ plants from the cross of PI 96354 × Bossier. The SNP genotypes of all samples determined by the LightCycler software agreed with previously determined SSR genotypes and the SNP genotypes determined on a Luminex 100 flow cytometry instrument. Multiplexed HybProbes for the two SNPs showed a 98.4% success rate and 100% concordance between repeats of two of the same 96 DNA samples. Also, we developed a HybProbe assay for the Rcs3 gene conditioning broad resistance to the frogeye leaf spot (FLS) disease. The LightCycler 480 provides rapid PCR on 384-well plate and allows simultaneous amplification and analysis in approximately 2 h without any additional steps after amplification. This allowed for a reduction of the potential contamination of PCR products, simplicity, and enablement of a streamlined workflow. The melting curve analysis on the LightCycler 480 provided high-throughput and rapid SNP genotyping and appears highly effective for marker-assisted selection in soybean. Melting curve analysis of fluorescently labeled DNA fragments is used extensively for genotyping single nucleotide polymorphism (SNP). Here, we evaluated a SNP genotyping method by melting curve analysis with the two probe chemistries in a 384-well plate format on a Roche LightCycler 480. The HybProbe chemistry is based on the fluorescence resonance energy transfer (FRET) and the SimpleProbe chemistry uses a terminal self-quenching fluorophore. We evaluated FRET HybProbes and SimpleProbes for two SNP sites closely linked to two quantitative trait loci (QTL) for southern root-knot nematode resistance. These probes were used to genotype the two parents and 94 F₂ plants from the cross of PI 96354 × Bossier. The SNP genotypes of all samples determined by the LightCycler software agreed with previously determined SSR genotypes and the SNP genotypes determined on a Luminex 100 flow cytometry instrument. Multiplexed HybProbes for the two SNPs showed a 98.4% success rate and 100% concordance between repeats of two of the same 96 DNA samples. Also, we developed a HybProbe assay for the Rcs3 gene conditioning broad resistance to the frogeye leaf spot (FLS) disease. The LightCycler 480 provides rapid PCR on 384-well plate and allows simultaneous amplification and analysis in approximately 2 h without any additional steps after amplification. This allowed for a reduction of the potential contamination of PCR products, simplicity, and enablement of a streamlined workflow. The melting curve analysis on the LightCycler 480 provided high-throughput and rapid SNP genotyping and appears highly effective for marker-assisted selection in soybean.