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LC-MS/MS를 이용한 반려동물 사료에서 곰팡이독소 동시분석법 유효성 확인
최윤화,안우석,김지은,김두환,Choi, Yoon Hwa,Ahn, Woo Seok,Kim, Ji Eun,Kim, Doo Hwan 한국동물위생학회 2022 韓國家畜衛生學會誌 Vol.45 No.3
The simultaneous analysis of mycotoxins using LC-MS/MS, a food official analysis method, was applied with compound feed for pets with high consumer preferences. In this study, the linearity of all calibration curves showed good linearity of 0.99 or more. and both the accuracy (recovery rate) and precision (repeatability) criteria of the concentration range for each mycotoxin in the National Agricultural Products Quality Management Service's Validation and Verification Guidelines were met. And as a result of analyzing FAPAS QCM in the same way, it was assesed that the z-scores of Aflatoxin B<sub>1</sub>, Ochratoxin A, Zearalenone, and Fumonisin B<sub>1</sub>, were within ±2 range. This study showed that the application of the food official analysis method to compound feed for pets is suitable.
식육 및 계란에서 플루오로퀴놀론계 항균물질 정량분석 및 잔류조사(2)
최윤화 ( Yoon Hwa Choi ),김연주 ( Yeon Ju Kim ),이경혜 ( Kyung Hye Lee ),강영일 ( Young Il Kang ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.3
Fluoroquinolones in muscle and egg were separated by liquid extraction and determined. The analysis was carried out using following conditions; C18 column (150×4.6mm, 5μm), mobile phase composed of D.W. (containing 0.4% triethylamine and phosphoric acid) : methanol : acetonitrile (780:100:120, v/v/v), quarternary pump at a flow rate of 0.9ml/min and 20μl of injection volume, fluorescence detector with EX 278nm/Em 456nm. The calibration range of seven fluoroquinolones showed linearity (r2?0.999) at concentration range of 0.025~0.8μg/ml. The recoveries in fortified muscle and egg represented more than 81.3%. The detection limits for ofloxacin, norfloxacin, ciprofloxacin, enrofloxacin, danofloxacin, saraloxacin and orbifloxacin were 3.1, 2.5, 3.6, 1.7, 0.9, 2.5 and 2.1μg/kg, respectively. We also monitored fluoroquinolones residue in the sample (chicken muscle 182, cattle muscle 140, pig muscle 139, egg 212) using EEC-plate (E. coli ATCC 11303) screening and HPLC confirmation methods. The screening test results, fluoroquinolones, antibacterial substances were all negative.
최윤화 ( Yoon Hwa Choi ),김연주 ( Yeon Ju Kim ),이경혜 ( Kyung Hye Lee ),강영일 ( Young Il Kang ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.3
This study was conducted to determine the content of heavy metals in meats available on the Korean markets. Trace metals (Pb, As and Cd) were detected in 4 kinds, 172 samples by inductively coupled plasma-mass spectrometry (ICP-MS). The values of heavy metals in meats were as follows; In beef, mean (minimum~ maximum) values of the heavy metals were Pb: 0.075 (0.020~0.190)mg/kg, As: 0.010 (0.001~ 0.050)mg/kg and Cd: 0.43(0.07~2.11)μg/kg. In pork, mean (minimum~maximum) values of the heavy metals were Pb: 0.065 (0.012~0.171)mg/kg, As: 0.004 (ND~0.021)mg/kg and Cd: 0.43(0.08~1.09)μg/kg. In chiken, mean (minimum~maximum) values of the heavy metals were Pb: 0.060(0.016~0.211)mg/kg, As: 0.008 (ND~0.042)mg/kg and Cd: 0.42 (0.02~3.80)μg/kg. In duck, mean (minimum~maximum) values of the heavy metals were Pb: 0.070 (0.011~0.157)mg/kg, As: 0.005 (0.001~0.011)mg/kg and Cd: 0.87 (0.15~3.75)μg/kg. This results will be used as a basic data for the future legislation on the regulation and control of heavy metal in meats.
최윤화 ( Yoon-hwa Choi ),김미란 ( Mi-ran Kim ),김영섭 ( Young-seob Kim ),이주형 ( Ju-hyeong Lee ) 한국가축위생학회 2017 韓國家畜衛生學會誌 Vol.40 No.1
We investigated the residues of β-agonist (zilpaterol, ractopamine and clenbuterol) using LC-MS/MS in raw meat in Seoul. The recoveries ranged between 76.7∼89.9% in beef respectively. The limits of detection were 0.01∼0.09 μg/kg and the limits of quantification were 0.03∼0.28 μg/kg respectively. Residues of β-agonist drugs which exceeded maximum residue limits (MRL) were not exceed in any of the 267 samples.
영어마을 단기 체험학습 프로그램이 초등학생의 영어학습 동기에 미치는 영향영어마을 단기 체험학습 프로그램이 초등학생의 영어학습 동기에 미치는 영향
최윤화(Choi, Yoon-Hwa) 팬코리아영어교육학회(구 영남영어교육학회) 2011 영어교육연구 Vol.23 No.2
This paper investigates how a short immersion program at English Village would affect elementary students’ learning motivation and whether the programs have different influences on the students’ motivation according to their English levels. For this, 100 students in Grade 6 were divided into three experimental groups according to their achievement test scores and their motivation was analyzed before and after the program based on Gardner’s AMTB(Attitude/Motivation Test Battery). The results of this study are as follows: 1) as a whole, the students’ attitude toward learning English changed positively after they participated in the short immersion program at English Village though the effect was not strong enough to change students’ motivation; and 2) only the middle level group’s motivation changed significantly while the high and low level groups showed no significant change in their motivation after participating in the program. These findings reveal that short immersion programs at English Village are not effective for all levels. Therefore, it is necessary to analyze students’ needs more precisely for the effectiveness of short immersion programs at English Village.
축산식품 중 비스테로이드성 항염증 약물의 잔류함량 모니터링
최윤화 ( Yoon Hwa Choi ),김연주 ( Yoen Joo Kim ),신방우 ( Bang Woo Shin ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.3
This study was conducted to determine the content of non-steroidal anti-inflammatory drugs (NSAIDs) in meats available on the Korean markets. The analysis was carried out using following conditions; C18 column (100×2.1 mm, 1.7 μm), mobile phase composed of DW (containing 0.1% formic acid): acetonitrile (containing 0.1% formic acid), binary pump at a flow rate of 0.3 ml/min and 5 μl of injection volume, MS/MS detector with ESI positive mode. The calibration range of five NSAIDs showed linearity (r2≥0.99) at concentration range of 3.125~200 μg/kg. The recoveries in fortified muscle more than 78.7~100.3%. The detection limits for meloxicam, ketoprofen, flunixin, carprofen and tolfenamic acid were 3.5, 1.6, 1.7, 9.8 and 4.8 μg/kg, respectively. We also monitored NSAIDs residue in cattle muscle 51 samples. The test results, NSAIDs were all not founded.
최효정 ( Hyo Jung Choi ),홍인석 ( In Suk Hong ),최윤화 ( Yoon Hwa Choi ),이윤희 ( Youn Hui Lee ),김창기 ( Chang Gi Kim ),이덕주 ( Duck Joo Lee ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.3
This study was carried out to investigate the sorts and the quantity of preservatives in 2,575 samples of 10 kind products which had been sold in the markets from 2004 to 2006. These analyses were done using HPLC. By the result of investigation, the most frequently detected preservative was sorbic acid in 222 cases. Detection rate of sorbic acid was 8.62%. Dehydroacetic acid(DHA) was detected in 8 cases among 36 butter samples and one of 278 sausage samples. Detection rate of DHA was 0.0035%. In detail, the detection rate was 71.5%(88/123) in dried meat, 22.2%(8/36) in processed butter, 17.3%(48/278) in sausage, 10.4%(17/163) in canned meat etc., 7.9%(49/619) in ham, 7.48%(19/254) in processed cheese and 4%(2/49) in bacon, respectively. The content range of sorbic acid and its average in each of the processed meat and milk products are as follows: ND~1.64g/㎏, 0.26 g/kg in dried meat, ND~1.34g/㎏, 0.114g/㎏ in sausage, ND~0.41g/㎏, 0.015g/㎏ in canned meat etc., ND~1.37g/㎏, 0.038g/㎏ in ham, ND~1.63g/㎏, 0.056g/㎏ in processed cheese, ND~0.57g/㎏, 0.022g/㎏ in bacon, respectively. Amount of detected DHA was less than 0.23 g/kg, and its average was 0.022 g/kg in processed butter.
소지방 중 잔류농약분석을 위한 액-액분배법과 modified QuEChERS법 비교
김연주 ( Yoen Joo Kim ),최윤화 ( Yoon Hwa Choi ),신방우 ( Bang Woo Shin ),이정학 ( Jung Hark Lee ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.4
This article described the comparison of a quick, easy, cheap, effective, rugged and safe (QuEChERS) sample preparation and the classical method established by National Veterinary Research and Quarantine Service (NVRQS) for the determination of pesticide residues in livestock products using GC-tandem mass spectrometry. The classical method by NVRQS used liquid-liquid partioning followed by evaporizing. The modified QuEChERS entailed extraction of 2 g sample with 15 ml acetonitrile containing 1% acetic acid followed by addition of 6 g anhydrous magnesium sulfate and 1.5 g sodium acetate. After centrifugation, 6 ml of the extract underwent a cleanup step (in a technique known as column-based solid phase extraction) using 400 mg each of C18 and primary secondary amine sorbents plus 1,200 mg magnesium sulfate. The quantitation of individual pesticides by both methods was based on tissue standard calibration curves with a correlation coefficient in excess of 0.98 for the 24 pesticides. The detection limits by the classical method were ranged 1.3∼5.0 μg/kg, with mean recoveries between 76.2% and 114.3% except aldrin (59.3%) and deltamethrin (63.6%). The detection limits by modified QuEChERS were ranged 0.3∼6.2 μg/kg, with mean recoveries between 68.0% and 114.3% except dimethipin (152.6%), chlorfenvinphos (138.1%), 4,4-DDT (61.5%), aldrin (60.4%) and chinomethionate (30.3%).
단보 : 서울지역 유통 식육 중의 세팔로스포린계 항균물질 잔류실태 조사
김미란 ( Mi Ran Kim ),최윤화 ( Yoon Hwa Choi ),최훈 ( Hoon Choi ),김두환 ( Doo Hwan Kim ),김영섭 ( Young Seob Kim ),이주형 ( Ju Hyung Lee ) 한국가축위생학회 2015 韓國家畜衛生學會誌 Vol.38 No.4
We investigated the residues of 6 cephalosporins (cefquinome, cephalexin, cephalonium, cefazolin, ceftiofur, cefuroxime) using LC-MS/MS in raw meat in Seoul. This method involves extraction of the residue from the meat by distilled water and methanol followed by a manual of residue analysis published by the National Institute of Food and Drug Safety Evaluation. The recoveries ranged between 74.71∼ 90.01% in beef, 73.37∼101.40% in pork and 70.87∼95.53% in chicken, respectively. The limits of detection were 0.0004∼0.0563 mg/kg, and the limits of quantification were 0.001∼0.169 mg/kg respectively. Residues of cephalosporins which exceeded maximum residue limits (MRL) were not exceed in any of the 287 samples. However, it is necessary to develop multi-method, which includes the active metabolites of ceftiofur.