분리된 백서 췌도의 인슐린 분비에 미치는 Atrial Natriuretic Factor 의 영향

전기엽(Ki Youp Jeon),최영숙(Young Suck Choi),박명철(Myung Chul Park),정숙현(Suck Hyun Jeong),정준용(Jun Yong Jeong),송미숙(Mi Suck Song),김은영(Eun Young Kim),이영화(Young Wha Lee) 대한내과학회 1991 대한내과학회지 Vol.41 No.2

The atrial natriuretic factors have their main function in the regulation of water and elecrolyte balance and blood pressure1-5). Recently ANF-like immunoreactivty has been shown to also occur in pancreatic islet cells10), and because several intrapancreatic peptides are known to have the capacity to regulate islet function', we therefore investigated the influences of synthetic rat ANF (3-28) on glucose-stimulated insulin secretion. After pancreatic islets isolation, they were perifused with solutions which contained 10 mmol glucose, ANF, carbachol and adrenoceptor blockades. Their secreted insulin values are shown in Tables 1 and 2, and their results are summarized as follows: 1) ANF significantly suppressed the glucose-induced increment of the first-and second-phase insulin secretion.(*p<0.05 ***p<0.0025) 2) ANF did not significantly suppress the chlinergic carbachol-induced increment of the first-phase insulin secretion. (p>0.05) 3) There was no demonstrable inhibitory effect of the ANF in the solution which contained combined a-and β-adrenoceptor blockade. In conclusion, we suggest that rat ANF inhibits glucose-stimulated insulin secretion in the rat by direct peculiar interferences and partial mediation of the adrenoceptors.

임상경과에 따른 쭈쭈가무시병의 면역양상

전기엽(Ki Youp Jeon),최영숙(Young Suck Choi) 대한내과학회 1994 대한내과학회지 Vol.46 No.5

Objectives: Tsutsugamushi disease is one of the important acute febrile illnesses with the high incidence during the fall throughout Korea. After year of 1986, its incidence showed a little increaseing tendency and occupied over 61% of the acute febrile illnesses during the fall in Chonbuck area. So for more understanding of this disease we observed immunologic features of the 35 Tsutsugamushi patients serially for 3 weeks after treatment. Methods: Tsutsugamushi patients were serially studied for total T cell, helper T cell, suppressor T cell, complement 3 and 4, natural killer cell, activated B cell by monoclonal antibodies CD3, CD4, CD8, CD16, CD20 and immunoglobulin G, A and M were measured by Turbidimetric Immuno Assay. With confirm diagnosis by Indirect Immunofluorescent Antibody test 35 patients were chosen and their values were analyzed and compared with the 25 normal control by computerized ANOVA T test. Results: Total T lymphocytes, helper T cell, helper/suppressor T cell ratio were significantly decreased (p<0.01) during one, two weeks after antibiotics treatment but helper T cell, helper/suppressor T cell ratio showed normal values three weeks after antibiotics treatment. Natural killer cell, immunoglobulin IgM, IgA were in- creased throughout the disease course with statistical significance (p<0.01) and complement C4 was increased until two weeks after antibiotics treatment (p<0.01). Activated B cell was increased significantly (p<0.01) two and three weeks after antibiotics treatment, IgG was significantly increased (p<0.05) during one, two and three weeks after antibiotics treatment. There was no demonstrable changes in the level of complement C3 and suppressor T cell during the disease course. Conclusion: Eventhough natural killer cell and elevated humoral immunity play recurperative roles in the early stage of the Tsutsugamushi disease, definitie recovery comes after significant changes of the cellular immunity induced by adequate antibiotics treatment. So it is suggested that cell mediated immunity play important roles in the recovery from the Tsutsugamushi disease and adequate antibiotics help our immune system to recurperate after this disease.

쭈쭈가무시병 98 예의 임상적 고찰 ( 1986 - 1991 )

전기엽(Ki Youp Jeon),최영숙(Young Suck Choi) 대한내과학회 1993 대한내과학회지 Vol.45 No.2

Background: Tsutsugamushi disease is one of the important acute febrile illnesses with the high incidence during the fall throughout Korea, especially in rural areas. Also in Chonbuck area, its prevalence is high and so we accumulated 98 cases from the year of 1986 to 1991 and observed their clinical characteristics. Method: The serologic patterns of the all 98 patients were studied as follows; Weil-Felix reaction was tested in the Serologic Diagnostic Departmemnt of the Korea National Institute of Health and Indirect Immunofluoscent Antibody test, Complement Fixation Test and IgM-specific dot-ELISA (enzyme linked immunosorbant assay) were tested in the Departement of Microbiology, Collage of Medicine, Seoul National University. Results: 1) There is annual increase of the tsusugamushi disease cases in Chonbuck area; 5 cases in the year of 1986, 19 cases in 1988, 20 cases in 1989, 23 cases in 1990 and 31 cases in 1991. 2) There was peak occurrence between October (41 cases, 42%) and November (56 cases, 57%), Large proportion of the patients were over 40 years old (67 cases, 69%). Patients were distributed throughout the whole area of Chonbuck province and the rate of male to female was 1: 1.25 (44 cases to 54 cases). 3) Illness was characterized by severe headache, fever, chillness, myalgia, maculopapula rashes, escar, and lymphadenopathies. Laboratory tests showed elevated abnormal liver function tests, lymphocytosis, elevated ESR, pyuria and proteinuria. 4) Treatment was so effectively carried out by the doxycycline, chloramphenicol or ulfamycin medication that clinical symptoms and signs were improved 2-3 days after onset of treatment. Conclusion: Tsutsugamushi disease is an important acute febrile illness in the Chonbuck province area, it has peak occurrence between October and November. There is tendency of increase in its occurrence, so we need adequate control method.

간생검조직에서 B 형 및 C 형 간염바이러스 유전자의 검출

전기엽(Ki Youp Jeon),최영숙(Young Suck Choi),조남훈(Nam Whoon Cho) 대한내과학회 1995 대한내과학회지 Vol.48 No.1

Objectives: It is known that about 8% and 1% of the Korean population is chronic carriers of hepatitis B virus (HBV) and hepatitis C virus (HCV), respectively. And hepatitis B and C viruses are associated with chronic carrier state and may cause chronic liver disease and hepatocellular carcinoma. For further understanding of the relationship between viral infections and liver diseases, it may be necessary to evaluate the presence of HBV-DNA and HCV-RNA in the liver tissues and we tried polymerase chain reaction (PCR) to detect them. Methods: We determined the HBV-DNA by PCR and HCU-RNA by the reverse transcription-polymerase chain reaction (RT-PCR) in the liver biopsy specimens of two patients with chronic hepatitis, six patients with liver cirrhosis and two patients with hepatocellular carcinoma. Results: 1) In the two liver biopsy specimens of chronic B hepatitis, HBV-DNA were positive and HCV-RNA were negative. 2) In the six freshly frozen liver specimens of liver cirrhosis, all of the six showed HBV-DNA positive and three of them showed HCV-RNA positive. 3) In two patients of hepatocellular carcinoma, one of them showed HBsAg positive, anti-HCV positive in serologic test, HBV-DNA and HCV-RNA were positive. And the other one showed HBsAg positive, anti-HCV negative, HBV-DNA positive and HCV-RNA negative in the freshly frozen liver tissues. Conclusion: Our findings demonstrate the feasibility of single PCR methods for the detection of HBV sequences and the feasibility of reverse transcription-single PCR technique for the detection of HCV sequences in freshly frozen liver tissues. And these techniques may reveal valuable information on the diagnosis of HRV infection and HCV infection and the pathogenesis of chronic viral hepatitis.

중합효소연쇄반응법 ( PCR ) 을 이용한 C 형 간염바이러스 RNA 의 검출

전기엽(Ki Youp Jeon),최영숙(Young Suck Choi),박영민(Yong Min Park),김부성(Boo Sung Kim) 대한내과학회 1994 대한내과학회지 Vol.47 No.3

Objectives: Hepatitis C virus (HCV) accounts for the occurrences of hepatocellular carcinoma, post-transfu- sion non-A non-B (NANR) hepatitis, sporadic NANB hepatitis and aggravates alcoholic liver disease. And we tred polymerase chain reaction (PCR) to evaluate hepatitis C viral infection. Methoda: We detected HCV RNA in the sera from patients with various liver diseases by PCR, to evaluate the replication and infectivity of HCU, And we compared HCV RNA positivity with anti-HCV positivity, Results: 1) In the 107 sporadic NANR liver diease patients, sixteen (15.0%) were anti HCV positive. In the 86 sporadic NANB liver disease patients, thirty-three (38,4%) were HCV-RNA positive. 2) In the 52 HBV-associated liver diasease patients, three (5.8%) were anti-HCV positive. In the 40 HBV- associated liver disease patients, three (12,5%) were HCV-RNA positive. 3) In the various liver diseases, positive rate of HCV- RNA by PCR was 2.1-2.6 times higher than that of anti-HCV. 4) In the total 13,488 blood donors for the three months of Red Cross Blood Center in Chonju, fourty-six (0.34%) were anti-HCV positive and sixteen (53%) among the thirty anti-HCV psitive sera were HCV-RNA positive. 5) Positive rate of anti-HCV of the blood donors, acute and chronic sporadic NANB hepatitis in Chonbuck area was much lower than that of Seoul area. But positive rate of HCV RNA by PCR was similar between two areas. Also positive rates of anti-HCV and HCV RNA in the NANB liver cirrhosis and NANB hepatodellular carcinoma were similar between two areas. Conclusion: By our results, it is suggested that the prevalence of C hepatitis may be underestimated by anti-HCV alone and so the detection of HCV-RNA by PCR is necessary for the true evaluation of HCV infection.

라우릴황산에스텔과 라우릴아마이드의 알킬롤황산에스텔과의 活性能에 관한 比較詩驗

金石萬,崔英淑 梨花女子大學校 韓國生活科學硏究院 1979 韓國生活科學硏究院 論叢 Vol.24 No.-

음이온성활성제는 고급지방산 또는 고급 알코올은 황상 easter型 또 sulornate 型 등 다양하며, 본 실험에서는 고급지방산에 직접 황상을 결합시킨 황상 ester과 간접으로 amide를 결합시킨 다음 황상 ester로 한 활성제의 활성능을 비교 시험한 결과 세정력, 경수성은 황상 ester가 약간 우수하고 침투력, 내산성을 amide 결합 황상 ester가 약간 우수하였다. A study of the comparative test of Sodium lauryl sufate and Sodium alkylol sulfate of laurylamide in their activities. The activities of Former are better in detergent power and hard water properties than the latter, but the latter is better in penetration and acidity.

무도병성 운동 및 일과성 양하지 마비를 보인 전신성 홍반성 낭창증 1 예

이춘희,전기엽,최영숙,이학송 대한내과학회 1990 대한내과학회지 Vol.39 No.4

This is an SLE case report of a 24-year-old male patient who had characteristic chorea movements of both upper extremties and transient paraplegia and who was observed at 3-year intervals. On September 17,1986, the patient complained of numbness and decreased mobility of the lower extremities and experienced difficulty in talking. He also complained of having floating foreign bodies in his eyes. The next morning, his mental capabilities decreased and he could not recognize his parents, the time of the day, nor places. A complete paraplegia of the lower extremities had set in along with choreic movements of both upper extremities and no ability to self-void. Even though he could not talk to us, he could understand spoken words and was very cooperative to teatment. With methylprednisolone therapy, September 20,1986, he was able to ambulate by himself without choreic movements of the upper extremities, his mental processes became clear and he was able to self-void. About 10 days later, he was discharged and there was no recurrence of his neurologic disorder until june 1990.

전북 지방의 C형 간염 발현 양상

이효구,김대곤,이수택,전기엽,최영숙,서재석 대한소화기학회 1992 대한소화기학회지 Vol.24 No.6

An RNA Virus, named hepatitis C Virus (HCV), has been proposed as an etiological agent for non-A, non-B (NANB) hepatitis, and antibodies directed to HCV (anti-HCV) are detectable by an enzyme-mmuno assay (EIA), and commercial first a'nd second generation assay kits are easily available. And so we planned this study in order to assess the causative role of HCV to the developmnt of the various liver diseases and to compare the prevalence of anti-HCV in Chonbuck province with that of the other areas. To evaluate the prevelence of anti-HCV in the pantieits with HBsAg negative and HBsAg positive liver diseases, we tested 347 sera with first generation of Abbott enzyme immuno assay method (HCV-EIA), and 121 sera samples with second generation of HCV-EIA. Among the total 468 patients, we selected 159 patients to make our goal because their diagnosis were biopsy proven and their anti-HCV were studied 2∼3 times serially. Anti-HCV was detected in fifty-two (11.1%) of 468 patients with various liver disaes, thirty-seven (10.7%) of 347 patients by first generation Kits, fifteen (12.4%) of 121 patients by second generation Kits and nineteen (12.0%) of selected patients. Among the nineteen anti-HCV positive cases, two (2.5%) of 79 patients with acute hepatitis, ten (16.4%) of 61 patients with chronic hepatitis, four (25%) of 16 patients with liver cirrhosis and one (33.3%) of 3 patients with hepatocellular carcinoma were positive for anti-HCV. Eleven (0.22%) of 4950 blood donors of the Chon Buck Red Cross Blood Center During the May, 1991, showed anti-HCV positivity. Ten 1:10 dilutions of 10 anti-HCV positive blood donor sera showed anti-HCV negative and ten 1:100 dilutions of 10 HBsAg positive blood donor sera showed HBsAg positive. Our data show that the prevalence of anti-HCV in Chon Buck area is low in patients with NANB acute and chronic hepatitis than that of other report. And the prevalence of anti-HCV in patients with NANB liver cirrhosis and hepatocellular carcinoma is similar to the other reports. The low concentration of the circulating antibody than the regional difference of C hepatitis viral antigen may be one of the reasons of it.