대학생의 혼자식사와 가공식품에 대한 인식 및 섭취 실태 조사

최병범,Choi, Byung Bum 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.6

This study was conducted to assess the recognition and consumption of meal alone and processed food according to major of college students in Seoul Metropolitan area and Chungcheong province, Republic of Korea. To accomplish this, a survey was conducted to investigate 353 college students (171 food & nutrition majors and 182 non-majors) regarding their general characteristics, dietary behaviors, meal alone issues, and the purchase and consumption of processed food. Most major and non-major students skipped breakfast. The main reason for skipping was not having time. Majors and non-majors put great importance on their lunch and evening meal, respectively. Nutrition information routes for majors were 'college class' (62.5%) and 'TV radio' (17.5%). However, the routes for non-majors to obtain nutrition information were 'internet smart phones' (41.1%) and 'TV radio' (28.3%). These results suggest the need to provide correct information concerning nutrition to college students. Most major and non-major students tend to have 0 to 2 times of meal alone per week. The main reason for meal alone was schedule unsuitable for eating with family or friends. The most critical factors for majors and non-majors when they chose menu to have meal alone were personal taste and money, respectively. The primary consideration for choosing processed food was price and the main purchase route was convenience store. The main reason for consuming processed food was easy-to-cook. Of processed foods, the most favored ones were noodles and fish products. Meal alone frequency was positively correlated with age (p<0.05), grade (p<0.05) and monthly allowance (p<0.05). Preference and consumption frequency of processed food were negatively correlated with concern about nutrition (p<0.05) and meal frequency (p<0.05), respectively. Based on these results, greater efforts should be made to provide meaningful information regarding programs for nutrition education to improve their dietary habits. Research and development of processed food specifically for college students eating alone should be increased.

Reversible Inactivation of Serratia Protease by 5,5'-dithiobis (2-nitrobenzoate)

최병범,김승수,Choi, Byung-Bum,Kim, Soung-Soo 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.3

Serratia marcescens ATCC 25419로 부터 균체의 단백질 분해효소를 정제하고 이 효소의 활성에 대한 여러가지 sulfhydryl reagent 및 단백질 modifying reagent들의 영향에 관하여 조사하였다. Sulfhydryl reagent 중에서 p-chloromercuribenzoate, N-ethylmaleimide, dithiothreitol은 효소의 활성에 아무런 영향을 미치지 않았으나 5,5'-dithiobis (2-nitrobenzoate) (DTNB) 만이 효소의 활성을 가역적으로 억제하였다. DTNB에 의한 효소활성의 억제는 DTNB의 농도가 높아짐에 따라 증가하였고, 10 mM DTNB를 효소와 3시간 동안 pre-incubation하였을 때 효소활성이 완전히 억제되었다. 한편 특정 아미노산과 반응하는 시약 중 dansyl chloride와 diethylpyrocarbonate는 효소의 활성에 아무런 영향을 주지 못하였으나, pyridoxal-5-phosphate와 phenylglyoxal은 효소의 활성을 급격히 감소시키는 점으로 보아 효소의 active site나 또는 그 근처에 lysine과 arginine이 존재할 가능성이 있는 것으로 나타났다. 또한 DTNB와 pyridoxal-5 phosphate 또는 DTNB와 phenylglyoxal을 효소와 함께 pre-incubation시키면 DTNB만 효소와 pre-incubation시켰을 경우보다 효소의 활성이 누적적으로 억제되었다. 여러 dicarboxylated compound 중 aromatic benzene ring을 가진 phthalate가 이 효소의 활성을 현저히 억제하는 사실로 보아 DTNB의 영향도 aromatic ring과 dicarboxyl group이 중요할 것으로 예상되며, 특히 효소의 lysine 및 arginine residue와 DTNB의 noncovalent interactions의 가능성이 더 연구되어야 한다. The effect of sulfhydryl reagents and some amino acid modifying reagents on the purified extracellular protease from Serratia marcescens ATCC 25419 was examined. Among several sulfhydryl reagents tested, the activity of the enzyme was reversibly inhibited by 5,5'-dithiobis (2-nitrobenzoate)(DTNB), with the inhibition increasing as the concentration of DTNB was increased. Dithiothreitol, p-chloromercuribenzoic acid, iodoacetamide, and N-ethylmaleimide had virturally no effect upon the reaction velocities of the enzyme. The protease was also inhibited by pyridoxal-5-phosphate and phenylglyoxal, which suggests that lysine and arginine are present at the active site or near the active site of the enzyme. Effector studies with various dicarboxylated compounds revealed that phthalate having dicarboxylated benzene ring inhibited the protease considerably, while other dicarboxylated compounds without benzene ring had no significant inhibitory effects on the enzyme activity. A concentration and time-dependent reversible inactivation of Serratia protease by DTNB was increased cumulatively when DTNB plus pyndoxal phosphate or DTNB plus phenylglyoxal were preincubated with the enzyme for a given time before enzyme assay. It remains to be established whether DTNB inactivation of Serratia protease is the result of direct noncovalent interactions of DTNB with either lysine or arginine in the active site pocket or whether there exists some other distinct mechanism of inactivation.

연구논문 : 경기 북부 지역 일부 대학생의 식습관과 식행동 조사

최병범 ( Byung Bum Choi ) 한국식품영양학회 2013 韓國食品營養學會誌 Vol.26 No.3

1. 본 연구의 조사 대상자인 경기 북부(의정부, 양주 동두 천)에 거주하는 대학생을 성별을 기준으로 구분하여 대학생의 식생활 습관을 알아보기 위하여 먼저 아침식사와 점심식식사의 석습관 등에 대하여 조사하였다. 이들 중 ‘매일 먹는다’ 라고 응답한 학생들(38.2, 31.6%)을 제외한 나머지 학생들을 대상으로 조사한 아침 결식의 가장 큰 이유로 ‘시간이 없어서’ 라고 응답하였고, 아침식사의 종류는 남학생과 여학생이 각각‘밥과 반찬’ 79.7,84.0%로 주식이 쌀인 우리내라는 아침 식사의 형태가 밥을 먹는 경우가 월등히 많았다. 점심식사는 아침식사와 달리 규칙적으로 하고 결식률이 낮은 것은 기존의 연구 결과와 유사하였다. 점심식사 장소는 남학생과 여학생 모두 교내 식당보다 외부 식당 이용이 휠씬 많았고, 점심 식사의 종류는 남학생과 여학생이 각각 ‘밥 위주 한식 70.8, 56.3%, ‘주억밥 및 볶음밥류’ 16.9.7.5%, ‘국수 면류3.4. 23.0% 의 순으로 나타나, 주먹 밥을 먹는 경우가 많았으냐, 남학생은 여학생과 다르게 ‘주먹밥 및 볶음밥류’의 섭취가 많았고, 여학생은 남학생과 달리 국수 면류’의 섭취가 많았다. 조사 대상자의 점심식사 비용은 남학생과 여학생이 각각 ‘4,000~­7,000원 미만 67.4,70.1%로 조사되고, 조사 대상자의 한 달 용돈금액이 남학생과 여학생 각각 ‘20~30만 원’ 24.7,31.6%, ‘10~20만 원‘ 21.3, 27.6%인 것을 볼 때 용돈에서 점심식사 비용이 대부분 차지하는 것을 알 수 있다. 2. 조사 대장자의 간식 습관을 알아보기 위하여 간식 섭취 빈도를 조사한 결과, 간식 섭취 횟수는 남학생과 여학생 각각 하루 1회 ‘47.2, 51.7%, 하루 2~3회 ‘31.5,39.7%의 순으로 나타냈다. 간식을 먹는 시간은 남학생과 여학생 각각 정심~저녁 사이’ 67.4, 67.8% 저녁식사 후’ 21.3, 8.6%, ‘아침~점심 사이’ 7.9, 14.4%로 나타났다. 간식을 하는 주된 이유를 살펴보면 남학생과 여학생 각각 ‘배가 고파서’ 46.1, 28.2%, ‘심심 해서’ 15.7, 15.5%, ‘습관적으로 9.0, 22.4%등으로 다양하게 나타났다. 주로 먹는 간삭의 종류를 살펴보면 남학생과 여학생 각각 ‘과자류 25.8. 19.0% ‘ 음료수’ 18.0, 14.4%, 빵류 16.9, 13.2% 순으로 과자류를 가장 선호하는 것으로 나타났고 야식 성취 횟수를 조사한 경과, 남학생과 여학생 모두 일주일에 1-2회’가 56.2, 62.1%로 가장 많았다. 3. 향후 젊은 세대가 선호하는 실버타운이나 노인복지 시설의 급식에 대한 기초자료 조사를 위하여 만 65세 이상의 노인이 되었을 때를 가정하여 조사한 식사 형태는 남학생과 여학생 각각 ‘직접 조리하여 먹기를 원한다.’ 48.9 48.9%, ‘주로 외식하기를 원한다. 20.2, 10.9%, ‘전문급식업체로부터 국이나 반찬을 배달하여 먹기를 원한다.’ 11.2, 21.8%의 순으로 나타났다. 노인이 되었을 때 정기적으로 음식을 제공받을 의향은 남학생과 여학생 각각 52.2, 55.7%이었으며, 이들 중 정기 배달식 의향이 있다고 응답한 화생들을 대상으로 조사한 배달식의 고려 사항은 남학생과 여학생 각각 “맛” 38.7, 24.7% 위생 35.5, 47.4% ‘영양’ 19.4, 22.7% 순이었다. 실버타운이나 노인복지시설의 시설급식 의향은 남학생과 여학생 각각 ‘예’ 47.2, 48.3%이며, 이들을 대상으로 조사한 시설급식을 원하는 이유로는 남학생과 여학생 각각 ‘비교적 저렴한 가격에 이용할 수 있어 경제적이다. ‘30.9, 22.6%, ‘식사 준비에서 해방 될 수 있다. 21.4, 23.8% 순이었고, 시설급식 의향이 없는 남학생과 여학생들을 대상으로 조사한 시설급식을 원하지 않는 이유로는 남학생과 여학생 각각 ‘실버타운이냐 노인복지 시설에 기거하고 싶지 않다’ 36.5, 45.6%, ‘다른 장소로 이동하여 먹고 싶지 않다’ 11.8, 10.0% 순이었다. This study was conducted to assess the dietary habits and behaviors of college students in the Northern Gyeonggi-do Region(Republic of Korea). To accomplish this, a survey was conducted to investigate the preference, intake frequency and menus of breakfast, lunch, dinner and snacks by male and female students. Most male and female students had irregular breakfasts with the main reasons for skipping a meal ‘not enough time’ (47.3, 45.5%) and ‘irritating’ (19.0, 21.9%), respectively. The principal breakfast menu for both was ‘rice and side dishes’. The lunch time for male and female students was regular, and the lunch cost made up most of their spending money. The snack time of male and female students was mostly ‘between lunch and dinner’. The snack menus of male and female students were ‘cookies’ (25.8, 19.0%), ‘drinks’ (18.0, 14.4%), and ‘breads’ (16.9, 13.2%), respectively. For male students, the reasons of snack intake were ‘hunger’ (46.1%), ‘boredom’ (15.7%), and ‘habitual’ (9.0%). For female students, the reasons were ‘hunger’ (28.2%), ‘habitual’ (22.4%), ‘boredom’ (15.5%). Half of the college students had regular delivery foods for reasons of ‘hygiene’, ‘taste’, and ‘nutrition’ in the order and in consideration of being the elderly. The facility foodservice was also used half of the college students for the reasons of being ‘economic’ (30.9, 22.6%), and ‘liberated from preparing meals’ (21.4, 23.8%), respectively. Both male and female students were found to have less knowledge of the facility foodservice in the elderly. Based on these results, greater efforts should be made to provide meaningful information regarding the dietary habits and behaviors of college students, especially, when elderly.

대중매체 영향에 따른 일부 대학생의 식품안전 지식 조사

최병범 ( Byung Bum Choi ) 한국식품영양학회 2011 韓國食品營養學會誌 Vol.24 No.2

This study was conducted to assess food safety knowledge of college students according to mass-media reports. To accomplish this, a survey was conducted to investigate the reliability, of primary mass-media sources on Bovine Spongiform Encephalopathy(BSE) as well as the preferences of college students. The most common sources of media consumed by male and female students were terrestrial television(TV)(60%), the internet(21%), and newspapers(11%) and terrestrial TV(60%), newspapers (18%), and the internet(17%), respectively. The preferences for media of major and non-major students were terrestrial TV(64%), newspapers(17%), and the internet(14%) and terrestrial TV(51%), the internet(22%), and newspapers(19%), respectively. The reliability of media as viewed by male students were as follows: terrestrial TV(53%) and the internet(40%), whereas for female students, these values were terrestrial TV(55%) and the internet(37%). The reliability of media as viewed by major and non-major students was as follows: terrestrial TV(67%) and the internet(28%), and terrestrial TV(44%) and the internet(44%), respectively. In the case of male students, the primary sources for information regarding BSE were terrestrial TV(54%) and the internet(38%), whereas for female students, the primary sources were terrestrial TV(57%) and internet (37%). In the case of major students, the primary sources for information regarding BSE were terrestrial TV(52%) and the Internet(40%), and for non-major students, the internet(47%) and terrestrial TV(39%). Both male and female students were found to have less knowledge regarding prions, SRM(Specified Risk Materials), MM(methionine-methionine) type genes, and cow above 30 months associated with BSE compared to the other factors associated with this disease. The BSE-related knowledge held by major and non-major students was revealed unknown more than the majority of responses. Based on these results, greater effort should be made to provide meaningful information to improve the food-safety knowledge of college students according to mass-media.

믹서의 종류에 따른 영양소 함량 변화에 대한 연구

최병범(Byung Bum Choi),김천흠(Chun Huem Kim),김영순(Young Soon Kim) 한국조리학회 2014 한국조리학회지 Vol.20 No.6

대학생의 식품 안전 관련 정보에 관한 연구

최병범 ( Byung Bum Choi ) 한국식품영양학회 2009 韓國食品營養學會誌 Vol.22 No.4

This study was conducted to assess the information of food safety by college students according to mass-media reports. To accomplish this, a survey was conducted to investigate the preference and reliability, of primary mass-media sources of BSE information, and BSE-related knowledge of college students. The most common sources of media by male and female students were terrestrial television(60%), the internet(21%) and newspapers(11%) and terrestrial television(60%), newspapers(18%) and the internet(17%), respectively. The reliability of media of male students were terrestrial television(53%) and the internet(40%), while for female students these values were terrestrial television(55%) and internet(37%), respectively. In the case of male students, the primary sources of information regarding BSE were terrestrial television(54%) and the internet(38%), but for female students the primary sources were terrestrial television(57%) and the internet(37%). Both male and female students were found to have less knowledge regarding prions, SRM, and MM type genes associated with BSE when compared to the other factors associated with this disease. In addition, the important determining factors for the food purchase of male and female students both were the orders of taste, hygiene, price. The preferences for meats and the beef-alternative meats of male and female students were the orders of pork, chicken. Based on these results, greater efforts should be made to provide meaningful information regarding the safe production and distribution of meats.

뉴클레오타이드에 의한 Serratia marcescens Catabolic Threonine Dehydratase의 활성화

최병범 ( Byung Bum Choi ) 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.2

Serratia marcescens catabolic threonine dehydratase는 streptomycin sulfate treatment, Sephadex G-20O gel filtration, AMP- Sepharose 4B affinity chromatography 등의 방법으로 정제하였는데,최종 단계에서 회수율은 15.5%이었으며 50배 정제되었다. Native 분자량은 native pore gradient polyacrylamide gel electrophoresis (PAGE) 방법으로는 120, 000이었다. SDS- PAGE 에 의한 subunit의 분자량은 30, 000이었고, 즉 S. marcescens 효소는 4개의 동일한 subunit으로 구성된 homo-tetrameric protein임이 판명되었다. S. marcescens 효소의 L-threonine에 대한 Km값은 AMP가 있는 조건에서 7.3 mM, AMP가 없는 조건에서 92 mM이었다. S. marceseens 효소는 효소 l mole 당 각각 2 mole의 Pyridoxal 5`-PhosPhate(PLP), l6개의 free-SH group을 가지고 있었다. S. marcescens 효소는 AMP의 존재 하에서 α-ketobutyrate, pyruvate, glyoxylate, phosphoenol pyruvate(PEP)에 의해 효소 활성이 억제되었으며, cAMP와 ADP에 의해서는 효소 활성이 증가되었다. 효소학적 성질 면에서 S. marcescens 효소는 E. coli 효소보다는 S. typhimurium 효소와 유사하였다. 한편, E. coli 효소는 cAMP에 의하여 효소 활성이 증가되고, S. typhimurium 효소는 ADP에 의해 효소 활성이 증가되는 것과 다르게, S. marcescens 효소는 AMP와 ADP 모두 효소 활성이 증가되었다. 따라서 이상의 연구 결과들은 세 enteric bacteria의 catabolic threonine dehydratase가 서로 작은 차이점이 있다는 것을 반영하며, 이러한 사실을 규명하기 위해서는 향후 보다 심층적인 연구를 수행하여야 할 것으로 사료된다.

쑥 추출물의 아미노산, 당 분석 및 항산화 효과에 관한 연구

최병범 ( Byung Bum Choi ),이혜정 ( Hye Jeong Lee ),방선권 ( Sun Kwon Bang ) 한국식품영양학회 2004 韓國食品營養學會誌 Vol.17 No.1

경기 북부 실버관리인력 양성 과정에 관한 수요도 조사

최병범 ( Byung Bum Choi ),김성후 ( Sung Hoo Kim ),이영주 ( Young Joo Lee ),유진현 ( Jin Hyun Yu ) 한국식품영양학회 2008 韓國食品營養學會誌 Vol.21 No.4

The principal objective of this study was to assess the demands of the development program of silver health care professionals for elderly individuals residing in the Northern Gyeonggi-do area. To this end, a survey was conducted to investigate college students and employees related with elderly individuals in the area regarding their recognition, interest, and involvement in the labor training program. In the case of college students, the health education they had received was only 1 to 2 hours, from a school lecture(35%). The content of health education they desired was exercise(34%) and stress management skills(28%). The sources of health information they received included mass media(77%) and the internet(12%), and they trusted the information they received from health professionals(45%), and the mass media(34%). In the case of health professionals who were working at silver care facilities, the sources of health information to which they had access were mass media(51%), internet(l4%), reliable health professionals(56%), mass media(22%), and books related to health(18%). The principal issues they reported as being relevant to the elderly were dementia(39%), hypertension(14%), arthritis(11%), and they reported that the most important personnel for elderly in the future would be care managers(44%), and care helpers(21%). 88% of subjects believed that there was a need for a silver welfare integrated information system. 43% of subjects used the internet, 77% of them required in-service training programs for the welfare of the elderly. Via this developmental program of silver health care professionals, a variety of new job opportunities can be provided in the future, and a program related to the silver service industry must be established as soon as possible.

경기북부 노인복지 전문인력에 대한 만족도 연구

최병범 ( Byung Bum Choi ) 韓國食品營養學會 2009 韓國食品營養學會誌 Vol.22 No.2

The principal objective of this study was to assess the demands and satisfactions of silver welfare professionals for elderly individuals residing in the Northern Gyeonggi-do area. To this end, a survey was conducted to investigate elderly individuals and employees related with them in terms of their recognition, interest, and involvement in a labor training program. The sources of health information the subjects received included mass media(50%) and health professionals(41%), and they tended to trust the information they received from health professionals(64%) and the mass media(26%). In the case of health professionals working at silver care facilities, the sources of health information to which they had access were: mass media(51%), health professionals(20%), internet(14%), reliable health professionals(56%), mass media(22%), and books related to health(18%). Elderly subjects` reasons for satisfaction with the facilities were as follows: access to meals(32%), elderly communities(24%), and good facilities(22%), whereas the reason subjects reported dissatisfaction with social difficulties(68%), bad facilities(20%), and programs(12%). The degrees of satisfaction of the respondents with the facility`s employees were reported as follows: life manager(28%), nurse(16%), and social worker(15%), whereas the subjects reported some degree of dissatisfaction with: nurses(29%), care helpers(17%), and facilities officers(13%). The priorities of the elderly welfare-related information were: disease and health(49%), daily life support(17%), nursing(11%), welfare facilities(8%), and the principal issues they reported as being relevant to elderly individuals were: palsy(16%), arthritis(14%), diabetes (12%), hypertension(10%), dementia(6%). They reported that the most important personnel for elderly in the future would be care managers(44%) and care helpers(21%). Via this developmental program of silver health care professionals, a variety of new job opportunities may be provided in the future, and a program related to the silver service industry must be established as soon as possible.