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천정녀,송인성,강동훈,송혜진,김혜인,서자원,이공주,김재상,강상원 한국분자세포생물학회 2008 Molecules and cells Vol.26 No.2
IκB kinase (IKK), the pivotal kinase in signal-dependent activation of nuclear factor-κB (NF-κB), is composed of multiple protein components, including IKK α/β/γ core subunits. To investigate the regulation of the IKK complex, we immunoaffinity purified the IKK complex, and by MALDI-TOF mass spectrometry identified a splice variant of zinc finger protein 268 (ZNF268) as a novel IKKinteracting protein. Both the full-length and the spliced form of the ZNF268 protein were detected in a variety of mammalian tissues and cell lines. The genes were cloned and expressed by In vitro transcription/translation. Several deletion derivatives, such as KRAB domain (KRAB) on its own, the KRAB/spacer/4-zinc fingers (zF4), and the spacer/ 4-zinc fingers (zS4), were ectopically expressed in mammalian cells and exhibited had different subcellular locations. The KRAB-containing mutants were restricted to the nucleus, while zS4 was localized in the cytosol. TNF-α- induced NF-κB activation was examined using these mutants and only zS4 was found to stimulate activation. Collectively, the results indicate that a spliced form of ZNF268 lacking the KRAB domain is located in the cytosol, where it seems to play a role in TNF-α-induced NF-κB activation by interacting with the IKK complex
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한강에서 분리된 Extended Spectrum β-Lactamase (ESBL) 대장균의 내성 연구
홍현진,천정녀,이연희 서울여자대학교 자연과학연구소 2003 자연과학연구논문집 Vol.15 No.-
Compared to the number of study with extended spectrum ,β-lactamases (ESBLs)producing clinical isolates, environmental ESBL-producers have not been extensively studied. To investigate environmental ESBL-producing Escherichia coli, twenty-two cephalothin-resistant E.coli were isolated from Han-river in Seoul, Korea. These isolates were resistant to ampicillin, cephalothin, and gentamicin and fourteen isolates among these were resistant to norfloxacin, too. All of these isolates produce AmpC and CMY, OXA, or TEM as determined by isoelectric point focusing gel electrophoresis and PCR. One isolate (No. 57-214) producing AmpC and OXA was resistant to all antibiotics (ampicillin, cephalothin, norfloxacin, gentamycin, cefotaxime, ceftazidime) tested in this study. Six isolates including isolate No. 57-214 could adhere to T24 human bladder cells and these isolates were not related to each other as shown with RAPD. ESBL을 생산하는 임상 균주에 대한 연구는 많은 연구가 진행되고 있는 데 비해 환경 균주에 대한 연구는 널리 진행되고 있지 않다. 환경에서 분리한 ESBL을 생산하는 E.coli를 연구하기 위해 서울의 한강에서 22개의 cephalothin 내성 E.coli를 분리하였다. 한강에서 분리한 22균주 모두 ampicillin, cephalothothin 그리고 gentamicin에 내성이었고, 이중 14 균주가 norfloxacin 에 내성이었다. PCR을 통하여 모든 균주가 AmpC와 CMY, OXA 또는 TEM을 가지고 있음을 확인하였고, 이들의 pI는 isoelectricfocusing을 통해 측정하였다. 특히, AmpC와 OXA를 생산하는 No.57-214는 본 실험에 사용한 모든 항생제에 내성을 보였다 (ampicillin, cephalothin, norfloxacin). 이들 균주들은 randomly amplified polymorphic DNA (RAPD) 를 이용하여 연관도를 분석한 결과 각각 모두 다른 균주임을 확인하였다.
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