해수에서 분리한 Vibrio sp. M-96 균주의 열감수성 alkaline phosphatase 성질

박문경,진덕희,김중균,공인수,김광현,홍용기,Park, Moon-Kyung,Jin, Deuk-Hee,Kim, Joong-Kyun,Kong, In-Soo,Kim, Kwang-Hyeon,Hong, Yong-Ki 한국생명과학회 1996 생명과학회지 Vol.6 No.3

A thermolabile alkaline phosphatase has been purified through steps of osmotic shock, ammonium sulfate salting-out, and DAEA-cellulose chromatography from the cultured broth of the marine Vibrio sp. M-96 strain. The optimal temperature for the enzyme activity was 35$\circ$C. The optimal pH was pH11.0, and the range of pHstability was pH10.4 to 12.0. Thermal inactivation occured within 6 mintes at 60$\circ$C. The enzyme was considerably inactivated by 0.1mM concentrations of Hg$^{2+}$, Ni$^{2+}$ and Zn$^{2+}$, whereas activated up to 234% by 1mM of Mn$^{2+}$. The activation energy and deactivation energy by the Arrhenius equation were 4.02 Kcal/mol and 9.098 Kcal/mol, respectively. The Km and Vmax values of the enzyme for p-introphenylphosphate were found to be 0.0465mM and 0.001334mM/min, respectively. Active form of the enzyme had a molecular weight of 57,000 dalton determined by the Sephadex G-200 gel filtration method.

넙치와 조기의 원산지 판별을 위한 random amplified polymorphic DNA 패턴 연구

강덕진,이석근,진덕희,최석정,Kang Duk-Jin,Lee Suk-Keun,Jin Deuk-Hee,Choi Suk-Jung 한국생명과학회 2006 생명과학회지 Vol.16 No.1

본 연구에서 넙치와 조기의 원산지를 판별하기 위한 도구로 RAPD PCR 방법의 가능성을 확인하였다. 넙치는 한국의 주문진 자연산, 통영 양식산, 거제 양식산 그리고 북한 자연산을 실험에 사용하였다. 조기는 한국산과 중국산을 사용하였다. 넙치의 RAPD 패턴에서는 뚜렷하고 일관성이 있는 진단용 띠들을 쉽게 찾을 수 있었다. 조기의 경우에는 유전적인 이질성으로 인하여 각 개체의 RAPD 패턴에서는 일관성이 있는 진단용 띠를 찾기 어려웠지만 각 원산지별로 얻은 RAPD 패턴에서는 가능성이 있는 진단용 띠들을 찾을 수 있었다. The random amplified polymorphic DNA (RAPD) technique was investigated as a potential tool for the origin identification of olive flounder (Paralichthys olivaceus) and redlip croaker (Pseudosciaena polyactis). Olive flounder specimens were collected from North Korea and several locations of South Korea (Jumunjin, Tongyoung and Geoje). Fishes obtained from Tongyeong and Geoje were cultured products. Redlip croaker specimens were collected from South Korea and China. Consistent and distinct diagnostic bands were easily identified in the RAPD patterns of the olive flounder specimens. Although consistent diagnostic bands rarely appeared in the RAPD pattern of redlip croaker specimens because of their genetic heterogeneity, we were able to find potential diagnostic bands in the average RAPD pattern of each origin.

해조류 김 Porphyra yezoensis 엽체로부터 산에 민감한 유전자의 분리

진형주(Hyung-Joo Jin),박선미(Sun-Mee Park),Long-Guo Kim,진덕희(Deuk-Hee Jin),공인수(In-Soo Kong),홍용기(Yong-Ki Hong) 한국생물공학회 2000 KSBB Journal Vol.14 No.6

Genetic Variations of Natural and Hatchery Populations of Korean Ayu (Plecoglossus altivelis) by Isozyme Markers

한현섭(Hyon-Sob Han),진덕희(Deuk-Hee Jin),이종관(Jong-Kwan Lee) 한국수산과학회 양식분과 2003 韓國養殖學會誌 Vol.16 No.2

Bunker - C 유 집식배양으로부터 해양세균 Plasmid 의 분포

박인식(In - Sick Park),박중연(Yung - Youn Park),진덕희(Deuk - Hee Jin),홍용기(Yong - Ki Hong) 한국미생물생명공학회 1988 한국미생물·생명공학회지 Vol.16 No.4

Effect of Scytosiphon lomentaria Ethanol Extracts on Myostatin Activity and Zebrafish Obesity Induced by High Feeding

Jun Gyo Jung(정준교),Jae Hong Kim(김재홍),Jeong Hwan Kim(김정환),Yong Soo Kim(김용수),Deuk-Hee Jin(진덕희),Hyung-Joo Jin(진형주) 한국생명과학회 2021 생명과학회지 Vol.31 No.8

생활습관 개선을 통한 근육량향상은 대사 증후군의 위험을 줄이는 것으로 보고되었다. 본 연구에서는 갈조류인 고리매(Scytosiphon lomentaria) 에탄올 추출물이 골격근 성장억제조절 단백질인 마이오스타틴 신호전달을 억제하는지와 고 급식으로 유도된 비만 제브라피시의 대사 항상성에 대한 효과를 확인하였다. 고리매 에탄올 추출물(10 ㎍/ml)은 pGL3- (CAGA) 12-루시퍼라제 분석에서 마이오스타틴(1 nM/ml) 신호를 완전히 차단하였다. 또한 웨스턴 블롯 분석에서 마이오스타틴 신호를 차단하여 Smad2 인산화가 억제되는 것을 확인하였다. 제브라피쉬의 치어에 대한 연구는 고 급식 대조군 그룹의 체내 포도당 농도는 정상 급식 대조군 그룹보다 유의하게 높았지만, 12.5 ㎍의 고리매 에탄올을 처리한 고 급식 그룹과 18.75 ug의 고리매 에탄올로 처리한 고 급식 그룹의 체내 포도당 수준은 정상 급식 대조군 그룹과 유사하였다. 고 급식 그룹의 GLUT2 유전자의 mRNA 발현 수준은 정상 급식 대조군 그룹에 비해 현저히 낮았다. 하지만, 고리매 에탄올 추출물을 처리한 실험군 그룹의 GLUT2 유전자의 발현은 정상 급식 대조군 그룹의 GLUT2 유전자의 발현과 거의 유사하게 나타났다. 그러므로 고리매 에탄올 추출물은 GLUT2유전자의 발현 조절을 통하여 체내 포도당 조절이 가능함을 보여준다. 본 연구의 결과는 고리매 에탄올 추출물이 대사 증후군 치료에 도움을 주는 소재 및 마이오스타틴 억제제로서의 가능성을 시사합니다. Muscle mass improvement through lifestyle modification has been shown to reduce the risk of metabolic syndrome. This study examined the capacity of ethanol extracts of Scytosiphon lomentaria (SLE) to suppress the bioactivity of myostatin, a potent negative regulator of skeletal muscle mass, as well as the effect of SLE treatment on metabolic homeostasis in obese zebrafish induced by high feeding. A total of 10 ㎍/ml SLE completely blocked myostatin (1 nM/ml) signaling in the pGL3-(CAGA)12 luciferase assay and suppressed myostatin-induced Smad2 phosphorylation in the Western blot analysis. In the zebrafish larvae analysis, the whole body glucose concentration of the high feeding control (HFC) group was significantly higher than that of the normal feeding control (NFC) group. However, the glucose levels of the high feeding group treated with 12.5 ㎍ SLE and of the high feeding group treated with 18.75 ug SLE were similar to those of the NFC group. The mRNA expression level of the GLUT2 gene of the HFC group was significantly lower than that of the NFC group. SLE treatment restored the expression of the GLUT2 gene to a level that was close to that of the NFC group, indicating that SLE is capable of regulating glucose levels in zebrafish larvae. The current results highlight the potential of SLE as a natural MSTN inhibitor and supplement that can be used to facilitate the treatment of metabolic syndrome.

연어의 모천회귀성에 관한 기초연구

성기백,진덕희 강릉대학교동해안지역연구소 1999 東海岸硏究 Vol.10 No.1

One synaptic structure which has a regulatory role in synaptic transmission in the postsynaptic density(PSD). Molecular and immunological studies have identified many regulatory proteins in the rat PSD. In this study, we have prepared PSD fractions from salmon(Chum Salmon, Oncorhynchus keta) brain(0.7g brain/3㎏ salmon). About 250㎍ of the crude PSD fraction could be prepared from an salmon brain. To study the protein composition and electrophoretic profiles, the PSD fraction prepared from salmon brains was analyzed in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). When the proteins were electrophoresed in an 6% SDS-gel, about 20 major distinct protein bands of 40-200kDa could be identified with Coomassie stain. In Coomassie stain, the overall protein profiles of salmon and rat PSD fraction were similar, but the concentration of protein bands were different.

온도 감수성 대장균의 형질전환에 있어서 Competent Cell의 보존효과

진덕희,홍용기,남주현 韓國自然史博物學會 1987 生物과 自然 Vol.17 No.2

온도감수성 대장균 M5248의 competent cell 보존효과에 4℃에서는 glycerol 및 DMSO를 첨가하지 않은 상태에서 24시간 보관시 3배정도 더 competent화가 되었으며 -20℃와 -40℃에서는 24시간 보관시 glycerol 및 DMSO의 유무에 관계없이 그 능력을 일정하게 보존시켰다. -40℃에서 볼 때 glycerol 및 DMSO를 첨가하지 않은 상태에서는 1주일만에 그 보존효과가 없어지는데 30% 혹은 40%의 glycerol 및 DMSO를 첨가하였을 경우 6개월 까지도 1/3정도 까지의 competent cell의 보존 능력을 보였다. The preparation of microbial cells permeable to DNA is typically a multistep procedure occupying the greater part of one thransformation experiment. Therefore a ready supply of competent cells can be of great convenience with a reduced procedure. The preservation of competent cells by freezing has been exploited in this work using a temperature sensitive mutant of Escherichia coliM5248. The transformation efficiency increased threefold after 24 hours-storage at 4℃ without any anticryogen, but the efficiency maintained to the original level at -20℃ or -40℃ with anticryogens such as glycerol or Dimethylsulfoxide. Addition of glycerol (30% or 40%) or Dimethylsulfoxide (30% or 40%) to allow freexing of the competent cells rendered susceptible to DNA till 6 months at -40℃, otherwise the competent state was disappeared within 1 week without the addition of anticryogens.

해수에서 분리한 Vibrio sp. M-96 균주의 열감수성 alkaline phosphatase 성질

박문경,진덕희,김중균,공인수,김광현,홍용기 동의대학교 기초과학연구소 1997 基礎科學硏究論文集 Vol.7 No.1

A thermolabile alkaline phosphatase has been purified through steps of osmotic shock, ammonium sulfate salting-out, and DEAE-cellulose chromatography from the cultured broth of the marine Vibrio sp. M-96 strain. The optimal temperature for the enzyme activity was 35℃. The optimal pH was pH 11.0, and the range of pH stability was pH 10.4 to 12.0. Thermal inactivation occured within 6 minutes at 60℃. The enzyme was considerably inactivated by 0.1mM concentrations of Hg^2+, Ni^2+ and Zn^2+, whereas activated up to 234% by 1mM of Mn^2+. The activation energy and deactivation energy by the Arrhenius equation were 4.02 Kcal/mol and 9.09 Kcal/mol, respectively. The Km and Vmax values of the enzyme for p-nitrophenylphosphate were found to be 0.0465mM and 0.001335mM/min. respectively. Active from of the enzyme had a molecular weight of 57,000 dalton determined by the Sephadex G-200 gel filtration method.

Sequencing of a 180 kDa Protein in the Rat Postsynaptic Density Fraction

Moon, Il Soo,Ko, Bok Hyun,Jin, Deuk Hee 東國大學校醫學硏究所 1995 東國醫學 Vol.3 No.-

신경세포들간의 신호전달은 연접이라고 불리는 특수한 접촉부위를 통하여 일어난다. 연접을 통한 신호전달의 효율은 고정된 것이 아니라 사용빈도와 사용형태에 따라 수정된다. 이러한 연접효율의 조절은 연접후요소가 중요한 역할을 한다. 연접후요소중 연접신호전달을 조절하는 기능을 갖는 중요한 구조는 PSD이다. 현재까지 분자생물학 및 면역학적인 방법을 이용하여 PSD내에 여러가지 신호전달물질이 있음이 밝혀졌다. 본 연구에서는 SDS-PAGE 및 최근에 개발된 아미노산배열순서결정방법을 이용하여 PSD에 존재하는 180 kDa 크기의 단백질(PSD-180)을 동정하였다. SDS-PAGE를 이용하여 PSD-180를 분리하고, Elutrap을 이용하여 electroelution하였는바 70%의 높은 수율을 보였다. PSD-180를 trypsin처리하고, 펲티드를 RP-HPLC로 분획하였다. 이들 분획중 3개를 순수분리하여 아미노산배열을 결정하고, 이를 이용하여 단백질정보은행에 조회한 결과 유사한 단백질을 발견하지 못하였다. 이는 PSD-180가 아직 보고되지 않은 새로운 단백질임을 시사한다. Nerve cells communicate through special contract sites called synapses. The efficiency of synaptic transmission is not fixed but modifiable depending on the frequency and pattern of usage. This synaptic regulation occurs at least in part by modulation of the postsynaptic elements. One synaptic structure which has a regulatory role in synaptic transmission is the postsynaptic density (PSD). Molecular and immunological studies have identified many regulatory proteins in the PSD. In the study, we have applied SDS-PAGE and recently developed protein microsequencing technologies to sequence an 180 kDa protein in the PSD fraction, termed PSD-180. The electroelution using an Elutrap device was very efficienct (70% yield). When the eluted protein was digested with trypsin and chromatographed on an reverse-phase HPLC column, it produced a very complex profile. Three peptides from HPLC peaks were purified and determined for amino acid sequences. Databank search did not match any known proteins indicating that PSD-180 is a new protein.