인삼 캘모듈린의 1차 구조

조경련,윤주억 ( Kyung Ryun Cho,Joo Ok Yoon ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.6

The complete amino acid sequence of calmodulin obtained from Panax ginseng was determined by sequencing the cyanogen bromide and arginylendopeptidase digested peptides of the purified Panax gingseng calmodulin. The calmodulin protein was found consisted of 149 amino acid residues and its amino-terminus was acetylalanine. Panax ginseng calmodulin did not contain tryptophan but contained 1 mol of histidine, tyrosine, cysteine, and Nε-trimethyl-lysine residues per mol of the protein. A comparison of its amino acid sequence with that of bovine brain calmodulin indicated that there were 13 amino acid substitutions and one insertion of amino acid residues in Panax ginseng calmodulin.

발아현미를 첨가한 백설기의 품질 특성

조경련 한국식품영양학회 2007 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2007 No.06

한천을 이용한 녹차 젤리에 관한 연구

조경련 한양대학교 2004 韓國 生活 科學 硏究 Vol.- No.23

The purpose of this study was to investigate the quality and sensory characteristics of green tea powder jelly. A central composite design involving green tea powder(5-15g) and sucrose(30-90g) was used to find the optimal condition of green tea powder jelly. Sensory characteristics, such as hardness and color overall quality of green tea powder jelly, were measured using instron and color difference meter. The elasticity, adhesiveness, chewiness, cohesiveness and overall quality were measured the panel test was taken. The overall optimal condition that satisfied all the sensory properties of green tea powder jelly were 15g green tea powder and 60g sucrose.

인삼 캘모듈린의 1차 구조

조경련,윤주억,Cho, Kyung-Ryun,Yoon, Joo-Ok 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.6

인삼 캘모듈린을 CNBr 및 arginylendopeptidase로 절단하여 펩티드 단편을 얻고, 이들 펩티드의 아미노산 서열을 분석하므로서 인삼 캘모듈린의 전 아미노산 서열을 결정하였다. 인삼 캘모듈린 단백질은 149잔기의 아미노산을 가지고 있였으며, 아미노-말단 아미노산은 아세틸화된 알라닌이었다. 인삼 캘모듈린에는 트립토판이 없었고, 1몰당 히스티딘, 티로신, 시스테인, $N_{\varepsilon}$-트리에틸리신 잔기가 각각 1몰씩 함유되고 있었다. 인삼 캘모듈린의 아미노산 서열을 소의 뇌 캘모듈린과 비교한 결과, 13잔기의 아미노산 치환과, 1잔기의 아미노산 삽입이 있었다. The complete amino acid sequence of calmodulin obtained from Panax ginseng was determined by sequencing the cyanogen bromide and arginylendopeptidase digested peptides of the purified Panax gingseng calmodulin. The calmodulin protein was found consisted of 149 amino acid residues and its amino-terminus was acetylalanine. Panax ginseng calmodulin did not contain tryptophan but contained 1 mol of histidine, tyrosine, cysteine, and $N_{\varepsilon}$-trimethyllysine residues per mol of the protein. A comparison of its amino acid sequence with that of bovine brain calmodulin indicated that there were 13 amino acid substitutions and one insertion of amino acid residues in Panax ginseng calmodulin.

우리나라 주민소환제 운용에 관한 연구

조경련(趙慶連),김영기(金渶淇) 한국지방정부학회 2008 지방정부연구 Vol.12 No.1

  우리나라는 선출직 지방공직자들의 일탈행동을 통제하기 위하여 주민소환법률을 제정하였다. 이 법의 초기 운용단계에서 법률의 운용을 둘러싸고 심각한 논란을 겪고 있다. 연구자들은 주민소환법 시행과 관련된 사실과 정보들을 수집하여 중요한 쟁점의 종류와 내용을 도출하기 위하여 자료를 분석하였다. 분석을 바탕으로 소환의 사유, 청원을 위한 서명유권자의 비율, 소환대상이 된 공직자의 직무권한 정지, 캠페인 비용부담의 주체 등이 중요한 쟁점이라는 것을 확인하였다.<BR>  쟁점들을 외국의 주민소환법 조항과 비교한 결과 정책제안을 제시하는 바, 1)주민소환의 사유는 무엇이든 적을 수 있도록 허용하는 포괄형 조항이 합리적이며, 2)서명의 비율은 선거구의 인구를 보다 정확하게 반영하는 조항으로 개선할 필요가 있고, 3)소환청구대상이 된 공직자의 직무권한은 해임이 결정될 때까지 유지하며, 4)공직자가 소환을 방어했을 때, 소환투표 캠페인에 투입된 비용은 지방정부의 재정으로 보전하는 것이 필요하다는 것이다.   The central government of Korea has enacted the Recall Law to control the deviant behaviors of local elected official"s. As it is, in the early stage of enforcing the Recall Act, we are experiencing a lot of critical controversies related to the exercise of the Law. We have gathered facts and information about the exercise of the Recall Act in Korea, and analyzed the datato depict the kind and contents of controversial issues. From the analysis, we could find such issues as the reason for the recall signature rate for petition, the provision of removing the official power of designated officers, campaign finances, etc.<BR>  After comparing these issues to the other country"s provisions of recall law, we suggest the policy proposals should be as follows; 1)it is reasonable, in Korea, to have the inclusive type provision that permits the voter to write anything about politics, if the matter is a political matter, such as a reason for recall petition, 2)the signature rate for petitions has to be improved to reflect the population size of wards for the designated officers more precisely and effectively, and 3)a designated officer should keep his or her job-related power till he or she is removed by a voter"s referendum, 4)the whole sum of campaign finances should be supported by the local government"s finances.

발아 현미를 첨가한 백설기의 품질 특성

조경련 ( Kyung Ryun Cho ) 한국식품영양학회 2007 韓國食品營養學會誌 Vol.20 No.2

부추 첨가 스폰지 케이크의 품질 특성 연구

조경련 ( Kyung Ryun Cho ) 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.4

This study was performed to investigate the quality characteristics of sponge cake made with leek(Allium tuberosum Rottler) powder. In order to evaluate the physical and sensory properties of different cake, the leek powder was added to wheat flour at various ratios(3, 5, 7, 9%, w/w). The specificgravity of bread dough tends to increase with the addition of leek powder. The moisture levels of the sponge cakes made with leek powder were higher than that of the control. Crumb color values, lightness(L) and redness(a) decreased by leek powder addition, while yellowness(b) was significantly increased (p<0.05). In texture analyses, hardness, cohesiveness, springiness andgumminess decreased with the addition of leek powder. In sensory evaluations, the 3% leek powder sponge cake showed higher sensory property scores than others(p<0.05).

브로콜리 분말을 첨가한 설기떡의 품질 특성

조경련 ( Kyung Ryun Cho ) 韓國食品營養學會 2009 韓國食品營養學會誌 Vol.22 No.2

Physical, textural and sensory properties of Seolgiddeok prepared with different amounts of broccoli(Brassica oleracea var. italica Plen.) powder were investigated during 3 days of storage. Moisture content decreased gradually during storage and was less in broccoli powder-amended samples. The color L value decreased significantly with increasing broccoli powder, whereas both redness and yellowness increased. Texture analyses revealed that hardness, chewiness, gumminess, adhesiveness and fracturability of Seolgiddeok tended to decrease in proportion to the amount of broccoli powder in the formula. Seolgiddeok gelatinization was investigated using amylographing. Break down and setback were low in broccoli powder Seolgiddeok. Sensory evaluations revealed that, Seolgiddeok prepared with broccoli powder was superior in flavor, chewiness, softness to unamended samples. Seolgiddeok prepared with 3% broccoli powder showed the highest overall acceptability score. Use of broccoli powder in Seolgiddeok preparation improves sensory characteristics and delays retrogradation.

콩 캘모듈린의 아미노산 서열

윤주억,조경련,변광의 ( Joo Ok Yoon,Kyung Ryun Cho,Kwang Eui Byoun ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.1

The complete amino acid sequence of calmodulin from soybean was determined by purifying and sequencing the cyanogen bromide and tryptic peptides. Soybean calmodulin consisted of 148 amino acid residues and its aminoterminus was blocked with an acetyl group. This calmodulin lacked tryptophan and contained one mol each of N ?trimethyllysine, histidine, and cysteine residues and two moles of tyrosine residues per mol of the protein. The comparison of the amino acid sequence of soybean calmodulin with that of bovine brain calmodulin indicated that there were nine amino acid substitutions other than amide assignments, one insertion and one deletion of amino acid residues in soybean calmodulin.

새롭게 합성한 크로모포릭 펩티드 기질에 의한 HIV - 1 프로테이자에의 효소반응속도 측정

윤주억,조경련 ( Joo Ok Yoon,Kyung Ryun Cho ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.4

HIV-1 protease hydrolyzed the newly synthesized peptides, Asn-Asn-Gln-Val-Phe (NO₂)-Val-Arg-NH₂ and acetyl-Arg-Lys-Leu-Val-Phe(NO₂)-Leu-Asp-Gly-NH₂ between the valyl and (p-nitro)phenylalanyl residues. The hydrolysis of these peptides resulted in a decrease in UV absorbance at 310 nm. The HIV-1 protease-catalyzed peptidolysis of these two peptide substrates was characterized by a linear time course at substrate turnover of ≤20%. The solubilities of these substrates at pH 4.7 were sufficient to perform initial rate measurements over a concentration range of 50 to 500 nM. Steady-state kinetic data and inhibition constants of the peptidolysis of these peptide substrates resulted in comparable values.