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Simple Isolation of DNA from the Tissue Homogenate by Acridinium Affinity Chromatography
석대은,정창희,김윤배,정윤수,Sok, Dai-Eun,Jung, Chang-Hee,Kim, Yun-Bae,Chung, Yun-Su Korean Society for Biochemistry and Molecular Biol 1990 한국생화학회지 Vol.23 No.3
고염농도(1.4 M NaCl)의 어류 근조직 균질액을 아크리디늄 친화성 컬럼에 흡착시킨 후 2M $MgCl_2$로 용출시켜 고순도의 DNA ($A_{260}/A_{280}$?2.0)를 얻었다. 이 컬럼에 흡착된 DNA는 0.5M $MgCl_2$로도 용출되었으나 2M NaCl로는 용출되지 않았다. 한편, DNase-I 처리로 친화성 컬럼상의 DNA의 크로마토그래피 유형이 달라졌으며, 이 친화성 컬럼은 단일 및 이중 나선형 DNA 모두에 좋은 결합 친화성을 가진 것으로 여겨진다. 따라서, 이 방법은 고이온강도에서 생조직 균질액으로부터 DNA를 분리하는데 적합할 것이다. The homogenate of fish muscle tissue in high salt concentration (1.4 M NaCl) was applied on the acridinium affinity column, and the elution of the column with 2 M $MgCl_2$ produced the material, which turned out to be a highly pure DNA ($A_{260}/A_{280}$?2.0). The DNA bound on the column was desorbed easily with 0.5 M $MgCl_2$, but not even with 2 M NaCl. Separately, the treatment with DNase-I altered the chromatographic profile of DNA on the affinity column, and the affinity column seems to express good binding affinity toward both single and double stranded DNAs. This method would be suitable for the separation of DNA from crude tissue homogenates in high ionic strength.
Soybean Lipoxygenase-Catalyzed Conversion of Arachidonic Acid into Lipoxins
석대은,피택산,정창희,정윤수,강정부,Sok, Dai-Eun,Phi, Taek-San,Jung, Chang-Hee,Chung, Yun-Su,Kang, Jung-Bu 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.2
아라키돈산에 대두 lipoxygenase를 작용시켰을때, 300-301 nm에서 강한 흡수를 나타내는 극성 물질들이 형성되었다. 이 물질들을 UV스펙트럼, HPLC, GC/MS에 의한 분석 결과, lipoxin A와 lipoxin B 이성체로 확인되었다. 이 방법은 수백 마이크로그람의 liposin A 및 B 이성체를 얻는데 편리한 것으로, lipoxins의 생합성과 생리적 활성을 연구하는데 유용할 것으로 사료된다. The exposure of arachidonic acid to soybean lipoxygenase led to the formation of polar products showing intense absorption at 300-301 nm. These products were found to be lipoxin A and B isomers, based on UV spectrum, HPLC and GC/MS analyses. This method, convenient to prepare hundreds of micrograms of lipoxin A and B isomers, would be useful for the study on the biosynthesis and the biological activity of lipoxins.
대두 lipoxygenase 에 의한 아라키돈산으로부터 lipoxins 으로서 전환
석대은,피택산,정창희,정윤수,강정부 ( Dai Eun Sok,Taek San Phi,Chang Hee Jung,Yun Su Chung,Jung Bu Kang ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.2
The exposure of arachidonic acid to soybean lipoxygenase led to the formation of polar products showing intense absorption at 300-301 nm. These products were found to be lipoxin A and B isomers, based on UV spectrum, HPLC and GC/MS analyses. This method, convenient to prepare hundreds of micrograms of lipoxin A and B isomers, would be useful for the study on the biosynthesis and the biological activity of lipoxins.
Simple Isolation of DNA from the Tissue Homogenate by Acridinium Affinity Chromatography
정윤수,정창희,석대은,김윤배 생화학분자생물학회 1994 BMB Reports Vol.23 No.3
The homogenate of fish muscle tissue in high salt concentration (1.4 M NaCl) was applied on the acridinium affinity column, and the elution of the column with 2 M MgCl₂ produced the material, which turned out to be a highly pure DNA (A_(260)/A_(280) 2.0). The DNA bound on the column was desorbed easily with 0.5 M MgCl₂, but not even with 2 M NaCl. Separately, the treatment with DNase-I altered the chromatographic profile of DNA on the affinity column, and the affinity column seems to express good binding affinity toward both single and double stranded DNAs. This method would be suitable for the separation of DNA from crude tissue homogenates in high ionic strength.
Hereditary Osteo-Onycho Dystrophy(HOOD) : (Nail patella Syndrome)의 1예
이기린,정창희 최신의학사 1972 最新醫學 Vol.15 No.9
Hood is a syndrome affecting mainly nails, elbows, pelvic and knees, and inherited as mendelian dominent trait with is probably mediated by a single mutant autosomal gene with variable expressivity. The name of HOOD was first used by Roeckerath in 1951, and was first described by Little in 1897. This syndrome has many synonyms such as Nail-patella syndrome, Fong's syndrome. Iliac horns syndrome. Onychoarthro-Osteodysplasia. Hereditary Onycho-Osteo Arthrodysplasia, Etc. The authors experienced a case of Hereditary Osteo-Onycho Dystrophy in 23 year old male. Survey was carried out on his family and found 3 positive cases among 8 members.
李起麟,丁昌熙 최신의학사 1972 最新醫學 Vol.15 No.10
Aneurysmal bone cyst is an uncomon tumor-like lesion of the bone, Conteining distended channels or cavities filled with blood. This Case, a twenty year old male, complained of pain and swelling in the left lateral aspect of the proximal tibia. Roentgenograms showed a balloned-out distension of the proximal tibia, eccentric buldging of the cortex and soap-bubble appearance of the lesion. The case was treated with radical curretage and autogenous bone graft with bone chips which were taken from the iliac wing. About 15 month later he bad no complaints and no evidence of recurrence.