辛夷花에 의한 卽時型 알레르기의 反應 抑制 效果

전창기,황충연,Jeon, Chang-Ki,Hwang, Chung-yeon 대한한방안이비인후피부과학회 1999 한방안이비인후피부과학회지 Vol.12 No.1

The mast cell plays a pivotal role in initiating allergic response by secreting intracytoplasmic granular mediators such as histamine. Magnoliae flos has been used for treatment of allergic disease in Korea. However, its effect in experimental models remains unknown. The present report describes an inhibitory effect of Magnoliae flos on mast cell-mediated immediate-type allergic reactions. Topical application of compound 48/80 can induce an ear swelling response in normal($WBB6F_1-+/+$) mice but not in the congenic mast cell-deficient $WBB6F_1-W/W^v$ mice. Magnoliae flos inhibited concentration-dependently mast cell-dependent ear swelling response induced by compound 48/80 by topical application. Magnoliae flos inhibited concentration-dependently passive cutaneous anaphylaxis induced by anti-dinitrophenyl (DNP) IgE in rats by topical application. Magnoliae flos also inhibited concentration-dependently the histamine release from the rat peritoneal mast cells by compound 48/80 or anti-DNP IgE. Moreover, Magnoliae flos had a significant inhibitory effect on compound 48/80-induced systemic anaphylactic reaction. These results indicate that Magnoliae flos inhibits immediate-type allergic reactions by inhibition of mast cell degranulation in vivo and in vitro.

과배란처리(過排卵處理)에 대(對)한 Mouse의 배란반응(排卵反應)의 계통차(系統差)

전창기,이시지마 요시,Jeon, Chang Ge,Ishijima, Yoshiro 충남대학교 농업과학연구소 1978 Korean Journal of Agricultural Science Vol.5 No.2

본(本) 연구(硏究)는 5계통(系統)(CF#1, C57BL/6, ICR, dd, CHN)의 mouse에 있어서 과배란처리(過排卵處理)에 대한 반응차(反應差)를 조사(調査)하기 위하여 수행(遂行)되었다. 과배란처리(過排卵處理)는 5IU의 PMS를 피하(皮下)에 주사(注射)한 다음 58시간(時間) 후(後)에 5IU의 HCG를 피하주사(皮下注射)하였으며, 배란수(排卵數)의 조사(調査)는 HCG 주사(注射) 24시간후(時間後)에 도살(屠殺)하여 난관내(卵管內)의 난자수(卵子數)를 현미경하(顯微鏡下)에서 계정(計定)하였다. 평균배란수(平均排卵數)는 CF#1, C57BL/6, ICR, dd 및 CHN계통(系統)에서 각각 21.3, 17.9, 20.7, 21.0 및 20.5개였다. The Present study was examined the response of mice of 5 strains to superovulation treatment. Female mice of CF#1, C57BL/6, ICR, dd and CHN strains, aged 60 days were used. Superovulation was induced by the subcutaneous injection of 5 IU PMS and followed 48 hours later by the subcutaneous injection of 5 IU HeG. All females were killed 24 hours after RCG and then ova in the oviduct were counted under a microscope. The average number of ova ovulated in CF#1, C57BL/6, ICR, dd and CHN were 21.3, 17.9, 20.7, 21.0 and 20.5, respectively.

P.M.S.에 의(依)한 가토(家兎)의 과잉배란(過剩排卵)에 있어서 Estrogen의 병용효과(倂用效果)

전창기,Jeon, C.G. 충남대학교 농업과학연구소 1976 Korean Journal of Agricultural Science Vol.3 No.1

가토(家兎)의 과배란유기(過排卵誘起)에 있어 P.M.S. 처리(處理)에 의(依)한 Estrogen의 병용효과(倂用效果)를 시험(試驗)하기 위(爲)해, P.M.S. 40I.U를 5일간(日間) 처리(處理)한것과, P.M.S.G. 40IU를 5일간(日間) 처리(處理)하고 최종일(最終日)에 Estrogen 0.1~0.5mg을 병용주사(倂用注射)한 것의 난소소견(卵巢所見)을 비교(比校)하며 배란수(排卵數)를 계측(計測)한 결과(結果), 다음과 같이 그 결과(結果)를 요약(要約)하였다. 1. P.M.S. 40IU를 5일간(日間) 피하주사(皮下注射)하여 24시간(時間) 및 48시간후(時間後)에 교미자극(交尾刺戟)을 준 대조구(對照區)의 평균(平均) 배란수(排卵數)는 21.3~24.1개(個), 과배란(過排卵) 양성률(陽性率) 50~63%에 비(比)하여 P.M.S.G 40, IU를 주사(注射)하고 최종일(最終日)에 Estrogen 0.1~0.5mg을 병용처리(倂用處理)한 구(區)의 평균(平均) 배란수(排卵數)는 32.5~37.8개과배란(個過排卵) 양성률(陽性率)은 87.5~100%로서 Estrogen을 병용처리(倂用處理)한 것이 배란수(排卵數)가 높아지는 경향(傾向)을 나타냈으며 미피열배란수(未破裂排卵數)는 대조구(對照區)가 많은 반면(反面), Estrogen 병용구(倂用區)는 적은 경향(傾向)을 나타냈다. 2. 대조구(對照區)와 Estrogen 병용구(倂用區)에 대(對)하여 난포(卵胞)를 1.0~1.4mm, 1.5~2.4mm, 2.5mm 이상(以上)의 3종(種)으로 분류(分類)한 후(後) 경시적(經時的) 관찰(觀察)에 의(依)한 발육정도(發育程度)를 비교(比較)한 결과(結果) 3종(種)에 대(對)한 배란수(排卵數)의 추이(推移)는 양구(兩區)가 서로 틀려서 총배란수면(總排卵數面)은 Estrogen 병용구(倂用區)가 우수(優秀)하였다 3. 이런 결과(結果)로 미루어 볼때 P.M.S. 주사(注射)에 대(對)한 Estrogen의 병용처리(倂用處理)는 외면적(外面的)으로는 난포발육(卵胞發育)에 대(對)하여 직접(直接) 영향(影響)을 주지 않으나 미피열난포(未破裂卵胞)를 줄여 배란수(排卵數)를 증가(增加)시키는 경향(傾向)을 나타냈다. This study was conducted to induce superovulation by P.M.S. injection and P.M.S. associate with Estrogen comparatively in the rabbit. The results obtained in this study are as follows. At 23-48 hours following mating, there were 21.3-24.1 ova (Ovulation rate was increased 50.5-63.6%) in average in the group of 40 I.U P.M.S. injected per day per herd for 5 days (total 200 I.U. of P.M.S.) and 32.5-37.8 ova (Ovulation rate was increased 87.5-100%) in the group of P.M.S. associats with 0.1mg at 5th day. She number of unovulated-follicle were fewer in P.M.S.-Estrogen group than PMS group. As the result that dia meter of ova were classified in 3 group as 1-1. 4mm, 1.5-2.4mm and 2.5mm and observed, the ova in P.M.S. Estrogen group were slightly larger in size than P.M.S. group. It was conclued that P.M.S. associate with Estrogen injection was not so much effect on growth of ova but effect on ovulation of ova. so the number of ovulated ova were increased than P.M.S. group.

조직배양액(組織培養液) 199배지(培地)에 의(依)한 가토난자(家兎卵子)의 저온(低溫)(5℃) 보존(保存)

전창기,이시지마 요시로,Jeon, C.G.,Isijima, Yosiro 충남대학교 농업과학연구소 1974 Korean Journal of Agricultural Science Vol.1 No.1

TC199 Medium를 Bas에 여러 양(量)의 가토혈청(家兎血淸)을 첨가(添加)하여 가토난자(家兎卵子)의 저온보존(低溫保存)를 시험(試驗)한 결과(結果) TC199단독(單獨)으로 $5^{\circ}C$에 48시간(時間) 보존(保存)한 16세포난자(細胞卵子)의 배양후(培養後)의 발생율(發生率) 31%에 대하여 TC199에 혈청(血淸)(20~80%)를 첨가(添加)했을때의 발생율(發生率) 80~100%라는 좋은 성적(成績)을 얻었다. 더욱이 비교혈청(比較血淸)만으로 생리적식염수(生理的食鹽水) 혈청(血淸)과 생리적(生理的) 식염수(食鹽水) 1:1의 Medium로 보존(保存)했을 때 이 발생율(發生率)은 100, 75, 및 83% 이였다. 이런 점(點)에서 TC199를 보존액(保存液)으로 사용(使用)했을 때에는 가토혈청(家兎血淸)의 첨가(添加)가 필요(必要)하다는 것이 판명(判明)되었다. Fertilized rabbit ova at 16-cell stage kept in TC 199, TC $199_+{^+}$ rabbitserum(4:1, 3:2, 2:3 and 1:4), rabbit serum, saline and serum+saline(1:1) were stored at $5^{\circ}C$ for 48 hours. They were than cultured at 37 C for 48 hours to determine their viability. The percentage of ova survived in each storage media was 31, 87, 100, 80, 100, 75 and 83%, respectively.

복수(腹水)에 의(依)한 가토난자(家兎卵子)의 체외배양(體外培養)에 관(關)한 연구(硏究)

전창기,이시지마 요시로,Jeon, C.G.,Isijima, Yosiro 충남대학교 농업과학연구소 1974 Korean Journal of Agricultural Science Vol.1 No.1

본시험(本試驗)은 과배란처리가토(過排卵處理家兎)에서 많은 양(量)이 채취(採取)할수 있는 복수(腹水)(peritoneal fluid)가 가토수정난자(家兎水精卵子)의 in. Vitro 배양(培養)의 배양액(培養液)으로서 이용(利用)할수 있는지를 검토(檢討)하기 위하여 실시(實施)하였다. 그 결과(結果)를 요약(要約)하면 다음과 같다. 2) 2세포(細胞), 4세포(細胞), 8세포(細胞), 16세포기(細胞期)의 가토난자(家兎卵子)를 복수(腹水)를 써서 $37^{\circ}C$에서 48시간(時間) 배양(培養)한 결과 분할(分割)된 난자(卵子)의 정도(程度)는 각각(各各) 78.1%, 83.3%, 91.7% 및 93.6%로서 이들의 성적(成績)은 가토혈청(家兎血淸)으로 배양(培養)했을 경우보다도 어느 것이나 좋았다. 2) 2세포(細胞)의 난자(卵子)를 복수(腹水)로 96시간(時間) 배양(培養)했을 때 배양난자(培養卵子)의 88%가 상실기이상(桑實期以上)으로 발달(發達)되고 24%가 배반포(胚盤胞)에 이르고 있으며 가토혈청(家兎血淸)으로 배양(培養)한 성적(成績)과 거의 같은 경향(傾向)을 보였다. 이상(以上)과 같은 점(點)에서 가토난자(家兎卵子)의 배양액(培養液)으로서 가토(家兎)의 복수(腹水)가 유효(有效)하라는 것이 입증(立證) 되었다. This experiment was studied on the peritoneal fluid wden it was used for nutrient solution of fertilized ova of rabbit when it was cultivated in vitro. The peritoneal fluid also can be gained much from the superovulated rabbits. The results was follow. 1. Wden 2. 4. 8. cell stage ova of rabbit were cultivated in peritoneal fluid in peritoneal fluid at 37'c for 48 Ths, each ova was developed by 78.1, 83.3, 91.7, and 93.6%, and these records were superior to when the blood serum was used. 2. When 2cell stage ova of rabbit was cultivated in peritoneal fluid 88% of Ova were repidly growth Far morula stage and 24 became blastocyst stage these records are nearly same to the records in morulel blood sevium. 3. At these ponts the rabbit peritoneal fluid can be used effectively for on culture fluid.

과배란처리(過排卵處理) 마우스에 있어서의 배란수(排卵數)의 좌우분포(左右分布)에 관한 연구(硏究)

전창기,이시지마 요시로,Jeon, Chang-ie,Ishijima, Yoshiro 충남대학교 농업과학연구소 1977 Korean Journal of Agricultural Science Vol.4 No.1

PMS와 HCG로 과배란유기(過排卵誘起)한 Mouse 배란수(排卵數)의 좌우난소(左右卵巢)의 관계를 비교하였다. 그 결과 배란수(排卵數) 1197개중(個中) 좌난소(左卵巢)에서 603, 우난소(右卵巢)에서 594개(個)의 배란(排卵)이 되고 있어서 동물전체(動物全體)로 보면 반반으로 되어 있다. 개체별(個體別)로 보면 좌우(左右)의 배란수(排卵數)가 많은 24예(例) 우측(右側)의 배란수(排卵數)가 많은 것은 23예(例) 좌우동수(左右同數)의 것은 3예(例)이었다. 또 좌우(左右) 배란수간(排卵數間)에는 뚜렷한 정(正)의 상관(相關)(ri=0.504, p<0.001)이 존재(存在)하였다. 본연구(本硏究)에 사용(使用)한 Mouse의 과배란성적(過排卵成績)은 저자(著者)와 석도(石島)가 1974년(年)1월(月)~4월(月)에 동경농업대학(東京農業大學) 가축번식연구실에서 공동(共同) 실험(實驗)한 것이다. 여기에 부기(附記)하여 관계 각위(各位)에 감사한다. The distribution of number of ovulation between right and left ovary in superovulated mice were examined. 50 adult female mice of CF#1 strain were used in this study. Superovulation was induced by the subcutaneous injection of 5 IU PMS and followed 48 hours later by the subcutaneous injection of 5 IU HCG. Females a were killed at 24 hours after injection of HCG, and ova in the oviducts were counted under a microscope. The number of ova ovulated from the left and the right ovary were 603 and 594. The number of ova in both sides were found equal frequency by $X^2$ test method. Ovulation occurred more frequently in the left ovary than in the right in 24 females and with equal frequency in both ovaries in 3 females, while more frequently in the right ovary than in the left in 23 females. The possitive correlation was found between right and left ovary.

축산공해(畜産公害)에 관(關)한 연구(硏究) -양돈업(養豚業)을 중심(中心)으로

전창기,김종우,라광연,김교준,Jeon, C.G.,Kim, J.W.,Ra, G.Y.,Kim, K.J. 충남대학교 농업과학연구소 1975 Korean Journal of Agricultural Science Vol.2 No.1

This excrement was conducted to investigate the nuisance of swine excrement. And excrements were collected from 27 heads of swine Euseong swine breeding center and Hongdo-dong swine farm and amounts of faces and urine, nitrogen, phosphorus, and kalium contents and number of parasite were investigated, and for the purpose to clean of excrementing matter, ferment substance and moist soil added to swine excreting matter and floating matter. Heat decresement amount and biological oxygen demand were investigated. And the result obtained were as follows. 1) Average amount of daily excreting faces per swine was 500-2700g from Euseong swine breeding center and 450-2500g from Hongdo-dong swine farm and a mount of excreting urine was 450-4500g from both of farms. The rate of excrements between faeces and urine was 1:1 2) Nitrogen contents were 0.802 ppm in urine and 0.514 ppm in faces, phosphorus were 2.261 % in urine and 0.073% in faeces and kalium contents were 1.094% in and 6.0467% in faeces. 3) Strongyloides ransomi and intestinal modular worm eggs showed the highest of eggs number was the result of observation in parasites, and ascaris showed the next it, whip worm and lung worm showed the lowest. 4) The effect ferment substance additive, amount of dry matter, floating matter, heat decreasement amount and biological oxygen demand was decreased but not significant was appeared. And the effect of moist soil additive, it was more effective that fermented substance additive for the fermentation of swine excrement. 돈분뇨(豚糞尿)의 공해(公害)를 조사(調査)하기 위(爲)하여 유성종축장(儒城種畜場) 홍도동양돈장(弘道洞養豚場)에서 사육(飼育)되고 있는 모돈(牡豚) 27두(頭)를 가지고 분뇨량(糞尿量), 질소(窒素), 인산(燐酸) 가리(加理) 함량(含量) 기생(寄生) 충란(蟲卵), 검사등(檢事等)을 실시(實施)하였으며 돈사오수(豚舍汚水)의 정화(淨化)를 목적(目的)으로 돈분(豚糞)에 발효제(醱酵齊) 오니등(汚泥等)을 첨가(添加)하고 정유물(淨游物), 증발잔유물(蒸發殘留物), 열감작량, B.O.D 등(等)을 검정(檢定)한 결과(結果) 다음과 같은 결과(結果)를 얻었다. 1) 분뇨량(糞尿量)은 1일평균(日平均) 유성종축장(儒城種畜場)이 최고(最高) 2700g, 최저(最低) 500g 이었고 홍도동양돈장(弘道洞養豚場)이 최고(最高) 4500g, 최저(最低) 450g으로서 분뇨비(糞尿比)는 1 : 1이었다. 2) 질소(窒素), 인산(燐酸) 가리(加理)를 분석(分析)한 결과(結果) 질소(窒素)는 분(糞)이 0.514 ppm, 뇨(尿)가 0.802 ppm, 인산(燐酸)은 분(糞)이 0.0739%, 뇨(尿)가 2.261%, 가리(加里)는 분(糞)이 0.0967%, 뇨(尿)가 1.094%이였다. 3) 분(糞)의 기생충란(寄生蟲卵)을 조사(檢査)한 결과(結果) 돈폐충(豚肺蟲) 및 장결절충란(腸結節蟲卵)이 가장 많고 회충(蛔蟲)이 다음이며 편충(鞭蟲)과 간충란(桿蟲卵)이 가장 적었다. 4) 발효제(醱酵齊)의 첨가효과(添加效果)는 무처리(無處理)에 비(比)하여 건조잔유물(乾燥殘留物), 부유물(浮留物), 열감작량, B.O.D 등(等)의 감량(減量)이 나타났으나 현저하지 못하였고 오니(汚泥)를 첨가(添加)할때의 분해효과(分解效果)는 발효제(醱酵齊)에 비(比)하여 상승(上昇) 되었다.

자우의 과배란

전창기,석도방랑 ( C . G . Jeon,Yosiro Isijima ) 한국축산학회 1975 한국축산학회지 Vol.17 No.1

복수에 의한 가토난자의 체외배양에 관한 연구

전창기,석도방랑 ( C . G . Jeon ) 한국축산학회 1974 한국축산학회지 Vol.16 No.4

This experiment was studied of the peritoneal fluid when it was used for culture fluid of fertilized ova of rabbits and it was cultivated in vitro. The peritoneal fluid also can be gained much from the superovulated rabbits. The results were as follow. 1. When 2, 4, 8 cell stage ova of rabbit were cultivated in peritoneal fluid at 37℃ for 48 hours each ova was developed as much as 78.1%, 83.3% and 91.7%, and these records were superior to the useing of blood serum. 2. When 2 cell stage ova of rabbit was cultivated in peritoneal fluid, 88% of ova were rapidly growth for morula stage and 24% became blastcyst stage. These records are nearly same to the records in blood serum. At these points, the rabbit peritoneal fluid can be used effectively for ova culture fluid.

과배란처리마우스의 착상후의 태아사망에 관하여

전창기,석도방랑 ( C . G . Jeon ) 한국축산학회 1974 한국축산학회지 Vol.16 No.4

This experiment was studied on the times of fetus-death during the gestation period of superovulated mows, and inspectation of implantation and living fetus number and the implantation points were done after 10, 12, 14, 16, 18 days from gestation. The results are as follows: 1. The coputaion rate was 68.1% (47/69) and the gestation rate was 68.1% (32/47) of superovulated mouse. 2. The average number of implantation was resulted like these, the 10 days after was 20.8, 12days after 18. 2, 14 clays after 17.1, 16 days after 17.0 and 18 days after 19.0, while the average number of living fetus were 10 days after 20.8 (100%: living fetus number/implantation number), 12 days after 16.3 (89.9%), 14 days after16.9 (98.3%), 16 days after 10.7 (62.7%) and 18 days after 10.2 (53.7%), and living fetus number was rapidly decreased from 16 days over. 3. The litter size of superovulated mouse was average 6.9 heads and did not over the control plot of which number is aveage 9.7 heads.