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성장속도에 따른 대장균 rnpB 유전자 발현의 조절작용
이영훈,정재훈,박충웅,이석재,전은순,이학란,정영환,조명선 한국유전학회 1993 Genes & Genomics Vol.15 No.1
Growth rate-dependent expression of the rnpB gene encoding M1 RNA, the RNA component of Escherichia coli RNase P, was examined using a hybrid transducing λ phage. The hybrid transducing λ phage was constructed carrying the lacZ gene fused to the promoter for the rnpB gene. The amounts of β-galactosidase were analyzed in the E. coli lysogen carrying this transducing phage grown in different growth media to see dependence of the rnpB transcription on growth rate. The synthesis of β-galactosidase from the rnpB-lacZ fusion increased with growth rate. This result suggests that the rnpB promoter activity is dependent on the growth rate.