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최청,구태호,장운빈,최희진,우희섭,손규목 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.5
The volatile compounds of Myungtae (Alaska pollack) sikhae obtained by simultaneous steam distillation and extraction(SDE) apparatus were separated by gas chromatography(GC) and gas chromatography mass spectrometry(GC/MS). The totals of 155 volatile flavor components was identified in traditional Kyungsangdo Myungtae (Alaska pollack) sikhae, respectively. α-Zingihirene(11.03%) (E)-di-2-propenyl disulfide(7.95%) β-cironellol(6.02%), methyl allyl disulfide(3.58%), cryptone(3.39%), camphene(3.23%), pentano(3.21%), penadecanal(2.66%) and β-phellandrene(2.06%) were contained as the main compounds of Myungatae shikae. The fraction obtained from sikhae were tested for electron donating ability, angiotensin converting enzyme and xanthine oxidase inhibitory activity. There was no electron donation abilities(SC_50) of hexane and water fraction. On the other hand, the abilities of ethylacetate fraction and butanol fraction showed 310.64 ㎍.mL, 1096.49 ㎍/mL, respectively. Angiotensin converting enzyme inhibitory activities(IC_50) of ethylacetate fraction and butanol fraction were 1.623 mg/mL, 1.303 mg/mL, respectively. Xanthine oxidase inhibitory activities(IC_50) of ethylacetate fraction and butanol fraction were 3.591 mg/mL, 2.803 mg/mL, respectively.
손준호,정명근,최희진,張云彬,배종호,이희덕,최청 한국농화학회 2002 Applied Biological Chemistry (Appl Biol Chem) Vol.45 No.3
한국산 검정콩 종피의 천연색소로의 이용가능성을 알아보기 위하여 온도, 당류, 유기산류, 비타민 C 그리고 금속 이온의 영향을 살펴보았다. 검정콩 색소의 열안정성은 다른 안토시아닌 계열의 색소와 비교할 ?? 유사하거나 더 안정한 것으로 나타났다. 당류의 영향에서는 타 색소와는 달리 농색화 현상은 일어나지 않았고 저장성에서는 모두 포도당에서 양호하였다. 유기산에서는 모든 첨가구에서 색의 강도가 증가하였으며 비타민 C 첨가의 경우 안토시아닌 색소에 부정적인 영향을 나타내었고 금속이온의 영향에서는 Cu^2+와 Zn^2+이온에서 안정하였으며 Mn^2+에서는 불안정하였다. 이상의 결과를 종합해 볼 때 검정콩 종피색소는 ph 3에서 다른 색소와 비교하여 상당히 안전한 색소임을 알 수 있었다. To examine utilization of Korean black soybean as a natural pigment, the effects of temperature, carbohydrate, organic acid, vitamin C and metal ion were investigated. Korean black soybean pigment was more stable than other anthocyanin pigments when temperature was abused. There was no darkness effect in carbohydrate.In organic acid, the color intensity was increased. The vitamin C addition negatively affected on color of anthocyanin. Among the metal ions tested, Cu^2+ and Zn^2+ was nor affected but Mn^@+ makes it unstable. Results indicate that Korean black soybean pigment was more stable than other anthocyanin pigment in various environment at pH 3.
손준호,정명근,최희진,장운빈,배종호,이희덕,최청,Son, Jun-Ho,Choung, Myoung-Gun,Choi, Hee-Jin,Jang, Un-Bin,Bae, Jong-Ho,Lee, Hee-Duck,Choi, Cheong 한국응용생명화학회 2002 한국농화학회지 Vol.45 No.3
한국산 검정콩 종피의 천연색소로의 이용가능성을 알아보기 위하여 온도, 당류, 유기산류, 비타민 C 그리고 금속 이온의 영향을 살펴보았다. 검정콩 색소의 열안정성은 다른 안토시아닌 계열의 색소와 비교할 때 유사하거나 더 안정한 것으로 나타났다. 당류의 영향에서는 타 색소와는 달리 농색화 현상은 일어나지 않았고 저장성에서는 모두 포도당에서 양호하였다. 유기산에서는 모든 첨가구에서 색의 강도가 증가하였으며 비타민 C 첨가의 경우 안토시아닌 색소에 부정적인 영향을 나타내었고 금속이온의 영향에서는 $Cu^{2+}$와 $Zn^{2+}$ 이온에서 안정하였으며 $Mn^{2+}$에서는 불안정하였다. 이상의 결과를 종합해 볼 때 검정콩 종피색소는 pH 3에서 다른 색소와 비교하여 상당히 안정한 색소임을 알 수 있었다. To examine utilization of Korean black soybean as a natural pigment, the effects of temperature, carbohydrate, organic acid, vitamin C and metal ion were investigated. Korean black soybean pigment was more stable than other anthocyanin pigments when temperature was abused. There was no darkness effect in carbohydrate. In organic acid, the color intensity was increased. The vitamin C addition negatively affected on color of anthocyanin. Among the metal ions tested, $Cu^{2+}$ and $Zn^{2+}$ was not affected but $Mn^{2+}$ makes it unstable. Results indicate that Korean black soybean pigment was more stable than other anthocyanin pigment in various environment at pH 3.
한국산 감잎로부터 Polyphenol계 생리활성물질 분리
안봉전,배만종,최희진,장운빈,성태수,최청,An, Bong-Jeun,Bae, Man-Jong,Choi, Hee-Jin,Zhang, Yun-Bin,Sung, Tae-Soo,Choi, Cheong 한국응용생명화학회 2002 한국농화학회지 Vol.45 No.4
옛부터 민간 약제로 사용했던 감나무 잎을 식품, 화장품 기능 생물 신소재로 활용하기 위해 감나무잎의 주성분으로 알려진 폴리페놀 화합물을 분리하고 생리활성 기능을 조사하였다. 감나무 잎을 60%의 아세톤으로 추출한 용액을 동결 건조시켜 농도별로 조제하고 glucosyltransferase, tyrosinase 효소저해를 관찰한 결과 glucoslytransferase는 $1.8{\times}10^{-1}$ mg/ml에서 82.84% 저해를, tyrosinase는 0.8 mg/ml에서 21,65% 저해 활성이 확인되었다. Sephadex G-50으로 gel filtration한 결과 fraction 1, 2, 3, 4, 5로 분획되었고 이중 fraction 1, 2에서 효소 저해 활성을 나타내었다. 활성 분획물의 물질군을 확인하기 위해 proteinase K 처리와 멸균처리한 결과 효소저해에 영향은 없어 활성물질군은 단백질군이 아니며, UV spectrum에서 자외선 영역에 최대 흡광치를 보여주므로 페놀링을 갖는 화합물로 추정하게 되었다. 추정화합물을 Sephadex LH-20, MCI-gel 및 Bondapak $C_{18}$ column에서 분리한 결과 F1 fraction에서 compound $1{\sim}4$가지 4종류의 화합물, F2 fraction에서는 compounds 5, 6, F3 fraction에서 compound 7, 8, 9를 순수 정제하여 재결정하였다. 분리된 화합물은 TLC 발색반응에서 flavan-3-ol 골격을 기본으로 하는 축합탄닌 반응을 보였고 전개정도에서 monomer가 2종류, dimer가 3종류, trimer가 1종류로 확인되었다. We purified polyphenols from persimmon leaf and tested their biological activity. The 60% acetone extract was lyophilized and applied to test enzyme inhibition of glucosyltransferase and tyrosinase. GTase was 82.4% inhibited at $1.8{\times}10^{-1}$ mg/ml and tyrosinase 21.7% inhibited at 0.8 mg/ml. The acetone extract was fractionated into F-1, 2, 3, 4, 5 by Sephadex Q-50 gel filtration and the fraction-1 and 2 showed higher enzyme inhibition activity than the other fractions. To the Proteinase K treatment and autoclaving of the two fractions had no effect on the enzyme activity, but these results suggested that active fraction was not protein but phenol ring completed compounds. By Sephadex LH-20, MCI-gel and Bondapak $C_{18}$ column chromatographies, compouds 1, 2, 3 and 4 from F-1 fraction, compounds 5 and 6 from F-2 fraction and compounds 7 , 8 from F-3 fraction were purified and re-crystallized. The purified compounds was assumed to be condensed tannins of frame flavan-3-ol frame on the basis of color reagent reaction and to be a mixture of monomer, dimer and trimer according to TLC analysis.
한국산 감잎로부터 Polyphenol계 생리활성물질 분리
안봉전 ( Bong Jeun An ),배만종 ( Man Jong Bae ),최희진 ( Hee Jin Choi ),장운빈 ( Yun Bin Zhang ),성태수 ( Tae Soo Sung ),최청 ( Cheong Choi ) 한국응용생명화학회 2002 Applied Biological Chemistry (Appl Biol Chem) Vol.45 No.4
We purified polyphenols from persimmon leaf and tested their biological activity. The 60% acetone extract was lyophilized and applied to test enzyme inhibition of glucosyltransferase and tyrosinase. GTase was 82.4% inhibited at 1.8 ×10 exp(-1) ㎎/㎖ and tyrosinase 21.7% inhibited at 0.8 ㎎/㎖. The acetone extract was fractionated into F-1, 2, 3, 4, 5 by Sephadex Q-50 gel filtration and the fraction-1 and 2 showed higher enzyme inhibition activity than the other fractions. To the Proteinase K treatment and autoclaving of the two fractions had no effect on the enzyme activity, but these results suggested that active fraction was not protein ut phenol ring completex compounds. By Sephadex LH-20, MCI-gel and Bondapak C_18 column chromatographies, compounds 1, 2, 3 and 4 from F-1 fraction, compounds 5 and 6 from F-2 fraction and compounds 7, 8 from F-3 fraction were purified and re-crystallized. The purified compounds was assumed to be condensed tannins of frame flavan-3-ol frame on the basis of color reagent reaction and to be a mixture of monomer, dimer and trimer according to TLC analysis.