PSCAD/EMTDC를 이용한 초전도 변압기 모델링

임채형(Chae-Hyung Lim),박민원(Min-won Park),유인근(In-Keun Yu) 대한전기학회 2003 대한전기학회 학술대회 논문집 Vol.2003 No.4

PSCAD/EMTDC를 이용한 3상 초전도 변압기 시뮬레이션

임채형(Chae-Hyung Lim),박민원(Min-won Park),유인근(In-Keun Yu) 대한전기학회 2003 대한전기학회 학술대회 논문집 Vol.2003 No.7

염화수은의 신장독성 지표 반응성 비교연구

김형섭 ( Hyung Sub Kim ),김용순 ( Yong Soon Kim ),임채형 ( Chae Hyung Lim ),곽승준 ( Seung Jun Kwack ),조영래 ( Young Rae Cho ),강미선 ( Mi Sun Kang ),오재호 ( Jae Ho Oh ),조완섭 ( Wan Seob Cho ),한범석 ( Bum Seok Han ),김승희 ( S 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.2

To compare the responsiveness of nephrotoxicity parameters, mercuric chloride(HgCl2) was administered intraperitoneally at the doses of 0.2, 0.6 and 1.0 mg / kg for different exposure periods(1, 3 and 7 days) using F344N rats. We compared several parameters resulted from animal study(body weight change, organ weight), clinical pathological(serum and urine chemistry), histopathological study and toxicogenomic study. Animal body weight significantly decreased and kidney weight(relative) increased in a dose-dependent manner. Serum chemistry analysis showed that BUN and creatinine were relatively one of nephrotoxicity biomarkers against HgCl2 and serum chemistry analysis was more sensitive than urine analysis. In the histopathological study, dose and time-dependency was clearly observed. Cellular swelling was observed in the low dose(0.2 mg / kg) group(1 day treatment) and focal inflammation including cellular swelling was observed in the high dose group. In the DNA microarray analysis, gene expression profiles were analysed in the 1-day and 3-days treatment group. Taken together, the responsiveness of individual parameter(body weight change, organ weight, clinical pathology, histopathology and DNA microarray) was different. This study suggests that these considerations should be involved in the analysis of toxicity study data as well as toxicogenomic study could provide a useful information to reduce the number of experimental animals used in the repeated toxicity study.

연구논문 : 염화수은의 신장독성 지표 반응성 비교연구

김형섭 ( Hyung Sub Kim ),김용순 ( Yong Soon Kim ),임채형 ( Chae Hyung Lim ),곽승준 ( Seung Jun Kwack ),조영래 ( Young Rae Cho ),강미선 ( Mi Sun Kang ),오재호 ( Jae Ho Oh ),조완섭 ( Wan Seob Cho ),한범석 ( Bum Seok Han ),김승희 ( S 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.2

To compare the responsiveness of nephrotoxicity parameters, mercuric chloride(HgCl2) was administered intraperitoneally at the doses of 0.2, 0.6 and 1.0 mg / kg for different exposure periods(1, 3 and 7 days) using F344N rats. We compared several parameters resulted from animal study(body weight change, organ weight), clinical pathological(serum and urine chemistry), histopathological study and toxicogenomic study. Animal body weight significantly decreased and kidney weight(relative) increased in a dose-dependent manner. Serum chemistry analysis showed that BUN and creatinine were relatively one of nephrotoxicity biomarkers against HgCl2 and serum chemistry analysis was more sensitive than urine analysis. In the histopathological study, dose and time-dependency was clearly observed. Cellular swelling was observed in the low dose(0.2 mg / kg) group(1 day treatment) and focal inflammation including cellular swelling was observed in the high dose group. In the DNA microarray analysis, gene expression profiles were analysed in the 1-day and 3-days treatment group. Taken together, the responsiveness of individual parameter(body weight change, organ weight, clinical pathology, histopathology and DNA microarray) was different. This study suggests that these considerations should be involved in the analysis of toxicity study data as well as toxicogenomic study could provide a useful information to reduce the number of experimental animals used in the repeated toxicity study.

IGBT를 이용한 새로운 Dimming 기법에 관한 연구

김보경(Bo-kyong Kim),임채형(Chae-hyung Lim),박민원(Min-won Park),성기철(Ki-chul seong),유인근(In-keun Yu) 대한전기학회 2002 대한전기학회 학술대회 논문집 Vol.2002 No.7

In vitro 아토피 피부염 유발 검색시험법 연구

주정흔 ( Jung Hun Ju ),임채형 ( Chae Hyung Lim ),김진호 ( Jin Ho Kim ),이정표 ( Jeong Pyo Lee ),손경희 ( Kyung Hee Son ),홍순근 ( Soon Keun Hong ),권태린 ( Tae Rin Kwon ),장미 ( Mi Chang ),김동섭 ( Dong Sup Kim ),윤혜성 ( Hae Seo 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1

Atopic dermatitis (AD) is a common pruritic disease and its incidence is increasing in infancy and childhood. AD is characterized by extreme pruritus and chronically relapsing inflammations and prevalence of AD has increased in recent years, but its exact etiology is unclear. Antigen-induced release of inflammatory mediators from mast cells causes the immediate symptoms of IgE-mediated allergic diseases, including allergic rhinitis, atopic dermatitis and atopic eczema. Mast cells rapidly release various allergic mediators, including histamine, cytokines that mediated acute and chronic allergic reaction. Since mast cells play a central role in the pathogenesis of various allergic diseases, we investigated the effect of exposure to 18 chemicals on the IgE-mediated allergic response in mast cells. We use two type of mast cells (RBL-2H3 cells, BMMC) to investigate the effects of chemicals on degranulation and cytokine production. Geraniol and formaldehyde significantly induced IgE-mediated mast cell degranulation. Dermatophagoides pteronyssinus allergen 1(DP) and dermatophagoides farinae allergen 1(DF) also induced degranulation without statistically significance. Also, we showed that release of IL-1β, IL-6 and GM-CSF was increased by treatment of geraniol. Also, We found that DP and DF induced release of IL-1β, IL-6, GM-CSF and TNF-α. These results suggest that in vitro method using mast cells degranulation and allergic cytokine secretion assay are useful for screening of atopic dermatitis trigger.

말초혈액을 이용한 초고속 유전독성평가법 개발 연구

안일영 ( Il Young Ahn ),김주환 ( Ju Hwan Kim ),임대현 ( Dae Hyun Lim ),양준영 ( Jun Young Yang ),김소영 ( So Young Kim ),이정선 ( Jung Sun Yi ),염영나 ( Young Na Yum ),임채형 ( Chae Hyung Lim ),이진하 ( Jin Ha Lee ),최기환 ( Ki Hw 한국동물실험대체법학회 2014 동물실험대체법학회지 Vol.8 No.1

To identify mutagenic potential of test substances, in vitro Ames tests are commonly used. Recently revised ICH S2(R1) guideline requires in vivo genotoxicity test if the result of the in vitro test is positive. In addition, a method testing multiple endpoints is required for animal welfare. Therefore we established a flow cytometry-based analysis such as Pig-a gene mutation assay and the micronuclei assay for detection of in vivo genotoxic potential using peripheral blood collected from repeated dose toxicity study. To evaluate these new methods, male Sprague Dawley rats were treated for 3, 14 or 28 days with N-nitro-N-ethylurea (ENU). ENU induced mutations in both reticulocytes (RET) and red blood cells of rats dose-dependently from the Pig-a gene mutation assay. ENU also increased micronucleated reticulocytes frequencies in flow cytometry based micronuclei assay, implying chromosomal damage to hematopoetic cells. These data show that both assays were well established. We additionally evaluated urethane and glycidol for applicability of Pig-a gene mutation assay and in vivo micronuclei assay by flow cytometry. Urethane, compared with vehicle control, did not increase Pig-a gene mutation and micronuclei frequency. Glycidol, compared with vehicle control, did not increase in micronuclei frequency, but Pig-a gene mutation significantly increased in the highest concentration for 28 days. Pig-a gene mutation assay for genotoxicity has many advantages: It can detect mutation in various species including humans, primates and rodents; and is integrated with repeated dose toxicity test without additional usage of animals; and has low spontaneous mutation frequency.

연구논문 : 말초혈액을 이용한 초고속 유전독성평가법 개발 연구

안일영 ( Il Young Ahn ),김주환 ( Ju Hwan Kim ),임대현 ( Dae Hyun Lim ),양준영 ( Jun Young Yang ),김소영 ( So Young Kim ),이정선 ( Jung Sun Yi ),염영나 ( Young Na Yum ),임채형 ( Chae Hyung Lim ),이진하 ( Jin Ha Lee ),최기환 ( Ki Hw 한국동물실험대체법학회 2014 동물실험대체법학회지 Vol.8 No.1

To identify mutagenic potential of test substances, in vitro Ames tests are commonly used. Recently revised ICH S2(R1) guideline requires in vivo genotoxicity test if the result of the in vitro test is positive. In addition, a method testing multiple endpoints is required for animal welfare. Therefore we established a flow cytometry-based analysis such as Pig-a gene mutation assay and the micronuclei assay for detection of in vivo genotoxic potential using peripheral blood collected from repeated dose toxicity study. To evaluate these new methods, male Sprague Dawley rats were treated for 3, 14 or 28 days with N-nitro-N-ethylurea (ENU). ENU induced mutations in both reticulocytes (RET) and red blood cells of rats dose-dependently from the Pig-a gene mutation assay. ENU also increased micronucleated reticulocytes frequencies in flow cytometry based micronuclei assay, implying chromosomal damage to hematopoetic cells. These data show that both assays were well established. We additionally evaluated urethane and glycidol for applicability of Pig-a gene mutation assay and in vivo micronuclei assay by flow cytometry. Urethane, compared with vehicle control, did not increase Pig-a gene mutation and micronuclei frequency. Glycidol, compared with vehicle control, did not increase in micronuclei frequency, but Pig-a gene mutation significantly increased in the highest concentration for 28 days. Pig-a gene mutation assay for genotoxicity has many advantages: It can detect mutation in various species including humans, primates and rodents; and is integrated with repeated dose toxicity test without additional usage of animals; and has low spontaneous mutation frequency.

인공피부모델과 사람, 돼지 피부의 피부흡수시험 비교 검증 연구

한주희 ( Ju Hee Han ),김시윤 ( C-yoon Kim ),임채형 ( Chae Hyung Lim ),나이랑 ( Yi Rang Na ),김태현 ( Tae Hyoun Kim ),정현 ( Hyun Jung ),석승혁 ( Seung Hyeok Seok ),정초록 ( Cho-rok Jung ),박재학 ( Jae Hak Park ) 한국동물실험대체법학회 2017 동물실험대체법학회지 Vol.11 No.1

This study was carried out in accordance with OECD guidelines on skin absorption test for the comparison of reconstructed human skin models, EFT-306™ and KeraSkinFT™ with human skin and pig skin. Three OECD standard compounds were used for penetration and absorption: Caffeine, benzoic acid and testosterone, all of which differ vastly in their lipophilicity. Reconstructed human skins were more permeable to low lipophilic compounds such as caffeine than human and pig skin. Differences became most obvious with KeraSkinFT™ showing a relatively weak penetration barrier on caffeine. EFT-306™ and KeraSkinFT™ both showed similar penetration patterns in the case of lipophilic compounds, benzoic acid and testosterone compared with human and pig skins. High correlation was observed on the penetration flux between the reconstructed human skin models and human and pig skin, even though different penetration rates existed. The reconstructed human skin models can be regarded as generally useful on lipophilic compounds for in-vitro penetration studies.

마이크로어레이 기술을 활용한 자외선 차단제의 주름생성 억제 연구

주정흔 ( Jung Hun Ju ),이종권 ( Jong Kwon Lee ),손경희 ( Kyung Hee Sohn ),임채형 ( Chae Hyung Lim ),홍순근 ( Soon Keun Hong ),김정래 ( Jung Rae Kim ),옥소원 ( So Won Ock ),김진호 ( Jin Ho Kim ),김인영 ( In Young Kim ),김승희 ( Seu 한국동물실험대체법학회 2008 동물실험대체법학회지 Vol.2 No.1

Ultraviolet(UV) irradiation damages skin and causes premature skin aging (photoaging) characterized by thickening, rough texture, coarse wrinkles and mottled pigmentation. A number of genes have been reported to be involved in the response to UV irradiation. However, these data are concentrated on a limited number of well-known genes and consequently limited in scope. Microarray technology has greatly facilitated the study of differential gene expression and allowed large-scale determination of gene expression changes associated with tissue changes and tumor formation. In the skin, microarrays have previously been used to study the basal gene expression that are sensitive or resistant to skin aging, and to examine differential gene expressions associated with the effects of UV irradiation. Many investigations have attempted to clarify the mechanisms induced by chronic UV irradiation, but the cellular and molecular events are not fully understood. To identify differential gene expressions associated with the effects of UV and sunscreen agent, we performed cDNA microarray analysis. We evaluated the differentially expressed gene profiles in UV-induced wrinkle and inhibitory effect of topical application of sunscreen agent on the formation of UVB-induced wrinkle. We identified functional gene sets including those involved in extracellular matrix, signaling, immunity and defense. There were significant changes of the gene expression in the mmp-13, laminin(beta), procollagen, ccl3, ccl4, cxcl10, ccl9, p16, caspase 9 etc. in the skin irradiation with UV.