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마이크로어레이 기술을 활용한 자외선 차단제의 주름생성 억제 연구
주정흔 ( Jung Hun Ju ),이종권 ( Jong Kwon Lee ),손경희 ( Kyung Hee Sohn ),임채형 ( Chae Hyung Lim ),홍순근 ( Soon Keun Hong ),김정래 ( Jung Rae Kim ),옥소원 ( So Won Ock ),김진호 ( Jin Ho Kim ),김인영 ( In Young Kim ),김승희 ( Seu 한국동물실험대체법학회 2008 동물실험대체법학회지 Vol.2 No.1
Ultraviolet(UV) irradiation damages skin and causes premature skin aging (photoaging) characterized by thickening, rough texture, coarse wrinkles and mottled pigmentation. A number of genes have been reported to be involved in the response to UV irradiation. However, these data are concentrated on a limited number of well-known genes and consequently limited in scope. Microarray technology has greatly facilitated the study of differential gene expression and allowed large-scale determination of gene expression changes associated with tissue changes and tumor formation. In the skin, microarrays have previously been used to study the basal gene expression that are sensitive or resistant to skin aging, and to examine differential gene expressions associated with the effects of UV irradiation. Many investigations have attempted to clarify the mechanisms induced by chronic UV irradiation, but the cellular and molecular events are not fully understood. To identify differential gene expressions associated with the effects of UV and sunscreen agent, we performed cDNA microarray analysis. We evaluated the differentially expressed gene profiles in UV-induced wrinkle and inhibitory effect of topical application of sunscreen agent on the formation of UVB-induced wrinkle. We identified functional gene sets including those involved in extracellular matrix, signaling, immunity and defense. There were significant changes of the gene expression in the mmp-13, laminin(beta), procollagen, ccl3, ccl4, cxcl10, ccl9, p16, caspase 9 etc. in the skin irradiation with UV.
주정흔 ( Jung Hun Ju ),이용경 ( Yong Kyoung Lee ),손경희 ( Kyung Hee Son ),이정표 ( Jeong Pyo Lee ),김진호 ( Jin Ho Kim ),임채형 ( Chae Hyung Lim ),홍순근 ( Soon Keun Hong ),권태린 ( Tae Rin Kwon ),장미 ( Mi Chang ),김동섭 ( Dong 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1
Phototoxicity is an acute toxic response induced by skin irradiation after the systemic or local administration of a chemical and subsequent exposure to light. Thus, it is necessary to evaluate phototoxicity of chemicals or products that are applied to the skin. Although the previous studies on the assessment of phototoxicity have been done using animals, it is necessary to establish alternative test methods, as EU ban animal-tested cosmetic ingredients or products. In vitro 3T3 NRU PT is an alternative method for identification of phototoxicity hazard chemicals as it compares cytotoxicity between UV irradiation and non-irradiation groups. Accordingly the accumulated information of phototoxicity assessment and its data has been found to be insufficient in Korea. Thus, we tested ten phototoxic and three non-phototoxic chemicals, and assessed two UV filter ingredients using in vitro 3T3 NRU PT mehtod. As a result, we identified that this method can be used to predict phototoxicity of chemicals. However, Eusolex 9020, UVA filter ingredient, was misclassified in vitro as a false positive compared with in vivo result. It suggests that other adequate test methods should be accompanied in order to enhance accuracy of phototoxicity prediction.
주정흔 ( Jung Hun Ju ),임채형 ( Chae Hyung Lim ),김진호 ( Jin Ho Kim ),이정표 ( Jeong Pyo Lee ),손경희 ( Kyung Hee Son ),홍순근 ( Soon Keun Hong ),권태린 ( Tae Rin Kwon ),장미 ( Mi Chang ),김동섭 ( Dong Sup Kim ),윤혜성 ( Hae Seo 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1
Atopic dermatitis (AD) is a common pruritic disease and its incidence is increasing in infancy and childhood. AD is characterized by extreme pruritus and chronically relapsing inflammations and prevalence of AD has increased in recent years, but its exact etiology is unclear. Antigen-induced release of inflammatory mediators from mast cells causes the immediate symptoms of IgE-mediated allergic diseases, including allergic rhinitis, atopic dermatitis and atopic eczema. Mast cells rapidly release various allergic mediators, including histamine, cytokines that mediated acute and chronic allergic reaction. Since mast cells play a central role in the pathogenesis of various allergic diseases, we investigated the effect of exposure to 18 chemicals on the IgE-mediated allergic response in mast cells. We use two type of mast cells (RBL-2H3 cells, BMMC) to investigate the effects of chemicals on degranulation and cytokine production. Geraniol and formaldehyde significantly induced IgE-mediated mast cell degranulation. Dermatophagoides pteronyssinus allergen 1(DP) and dermatophagoides farinae allergen 1(DF) also induced degranulation without statistically significance. Also, we showed that release of IL-1β, IL-6 and GM-CSF was increased by treatment of geraniol. Also, We found that DP and DF induced release of IL-1β, IL-6, GM-CSF and TNF-α. These results suggest that in vitro method using mast cells degranulation and allergic cytokine secretion assay are useful for screening of atopic dermatitis trigger.
연구논문 : 3T3 세포를 이용한 광독성 대체시험법 확립
주정흔 ( Jung Hun Ju ),이용경 ( Yong Kyoung Lee ),손경희 ( Kyung Hee Son ),이정표 ( Jeong Pyo Lee ),김진호 ( Jin Ho Kim ),임채형 ( Chae Hyung Lim ),홍순근 ( Soon Keun Hong ),권태린 ( Tae Rin Kwon ),장미 ( Mi Chang ),김동섭 ( Dong 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1
Phototoxicity is an acute toxic response induced by skin irradiation after the systemic or local administration of a chemical and subsequent exposure to light. Thus, it is necessary to evaluate phototoxicity of chemicals or products that are applied to the skin. Although the previous studies on the assessment of phototoxicity have been done using animals, it is necessary to establish alternative test methods, as EU ban animal-tested cosmetic ingredients or products. In vitro 3T3 NRU PT is an alternative method for identification of phototoxicity hazard chemicals as it compares cytotoxicity between UV irradiation and non-irradiation groups. Accordingly the accumulated information of phototoxicity assessment and its data has been found to be insufficient in Korea. Thus, we tested ten phototoxic and three non-phototoxic chemicals, and assessed two UV filter ingredients using in vitro 3T3 NRU PT mehtod. As a result, we identified that this method can be used to predict phototoxicity of chemicals. However, Eusolex 9020, UVA filter ingredient, was misclassified in vitro as a false positive compared with in vivo result. It suggests that other adequate test methods should be accompanied in order to enhance accuracy of phototoxicity prediction.
사람피부와 돼지피부를 이용한 OECD Guideline 428에 따른 피부흡수시험법 검증
한주희 ( Ju Hee Han ),석승혁 ( Seung Hyeok Seok ),백민원 ( Min Won Baek ),김동재 ( Dong Jae Kim ),나이랑 ( Yi Rang Na ),박귀례 ( Kui Lea Park ),손경희 ( Kyung Hee Sohn ),주정흔 ( Jung Hun Ju ),임채형 ( Chae Hyung Lim ),박재학 ( Ja 한국동물실험대체법학회 2009 동물실험대체법학회지 Vol.3 No.1
In vitro skin absorption test has been proposed as a reliable alternative method to in vivo skin absorption test. In many other countries, in vitro skin absorption has been conducted according to OECD guideline which was adopted in 2000. However, in vitro skin absorption test hasn`t been validated yet in Korea. In this study, experimental conditions were set and validated according to a detailed protocol of OECD guideline 428. We compared penetration properties of caffeine (log P -0.01) and benzoic acid (log P 1.83) through human split-thickness skin and pig ear full-thickness skin using Franz cell. The permeation of caffeine through the human skin (10.16%) was higher than through pig ear full-thickness skin (1.68%). The permeation of benzoic acid through human skin and pig ear skin were similar. Hydrophilic caffeine penetrated the human split-thickness skin more efficiently than pig ear full-thickness skin. Our data showed a similar pattern with compared other reports that regarded skin absorption of caffeine and benzoic acid through split-thickness skin and full-thickness skin. In conclusion, we validated in vitro skin absorption test in Korea
사람피부와 돼지피부를 이용한 OECD Guideline 428에 따른 피부흡수시험법 검증
한주희 ( Ju Hee Han ),석승혁 ( Seung Hyeok Seok ),백민원 ( Min Won Baek ),김동재 ( Dong Jae Kim ),나이랑 ( Yi Rang Na ),박귀례 ( Kui Lea Park ),손경희 ( Kyung Hee Sohn ),주정흔 ( Jung Hun Ju ),임채형 ( Chae Hyung Lim ),박재학 ( Ja 한국동물실험대체법학회 2009 동물실험대체법학회지 Vol.3 No.1
In vitro skin absorption test has been proposed as a reliable alternative method to in vivo skin absorption test. In many other countries, in vitro skin absorption has been conducted according to OECD guideline which was adopted in 2000. However, in vitro skin absorption test hasn`t been validated yet in Korea. In this study, experimental conditions were set and validated according to a detailed protocol of OECD guideline 428. We compared penetration properties of caffeine (log P -0.01) and benzoic acid (log P 1.83) through human split-thickness skin and pig ear full-thickness skin using Franz cell. The permeation of caffeine through the human skin (10.16%) was higher than through pig ear full-thickness skin (1.68%). The permeation of benzoic acid through human skin and pig ear skin were similar. Hydrophilic caffeine penetrated the human split-thickness skin more efficiently than pig ear full-thickness skin. Our data showed a similar pattern with compared other reports that regarded skin absorption of caffeine and benzoic acid through split-thickness skin and full-thickness skin. In conclusion, we validated in vitro skin absorption test in Korea
1% Zinc Pyrithione 샴푸의 비듬 치료 효과에 관한 연구
박주희 ( Ju Hee Park ),김범준 ( Beom Joon Kim ),김명남 ( Myeung Nam Kim ),홍연표 ( Yeon Pyo Hong ),주정흔 ( Jung Hun Ju ),이정표 ( Jung Pyo Lee ),손경희 ( Kyung Hee Sohn ),박귀례 ( Kui Lea Park ),이은창 ( Eun Chang Lee ),천영진 ( 대한피부과학회 2009 대한피부과학회지 Vol.47 No.8
Background: Dandruff is a common complaint, and is suffered by up to 50% of the population at some time. Malassezia yeasts, which comprise part of the normal skin flora, might be a critical factor in this disease, as they have been found in higher proportions in patients with seborrheic dermatitis or dandruff, its milder form. Objective: The aim of this study was to evaluate the clinical efficacy of 4 weeks of treatment with 1% zinc pyrithione (ZP) shampoo. Methods: A randomized, double-blind, 4-week treatment period was preceded by a 1-week run-in period. A total of 30 patients were enrolled in this study. Assessments included the patient`s subjective score (PSS) and the investigator`s assessment score (IAS), images of the affected scalp area, the severity of sebum production, and the erythema and moisturizing effect of the shampoo. Results: 1% ZP shampoo significantly reduced the extent and severity of scaling, as measured by folliscope imaging on visit 2 (p=0.0391) and visit 3 (p=0.0381), as well as pruritus related to the disease as measured by the grading systems, PSS (p=0.0352) and IAS (p=0.0142). Additionally, the results of this study show that a treatment regimen with 1% ZP shampoo significantly reduced scalp sebum production as measured by a sebumeter. Erythema measured by the chromameter was not as meaningful. The corneometric values were slightly increased in the group treated with 1% ZP shampoo but not in the group treated with ZP-free shampoo. Side effects of the ZP shampoo were quite mild and tolerable, and were observed only in a small group of patients. Conclusion: 1% ZP shampoo appears to be both effective and well-tolerated when used for the treatment of dandruff. (Korean J Dermatol 2009;47(8):875~883)
연구논문 : BrdU 면역조직화학염색을 이용한 국소 림프절 시험법(Local Lymph node assay) 연구
이종권 ( Jong Kwon Lee ),주정흔 ( Jung Hun Ju ),김정래 ( Jung Rae Kim ),손경희 ( Kyung Hee Sohn ),김진호 ( Jin Ho Kim ),김인영 ( In Young Kim ),전은영 ( Eun Young Jun ),김승희 ( Seung Hee Kim ),박귀례 ( Kui Lea Park ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.1
Allergic contact dermatitis (skin sensitization) may be caused by a wide variety of chemicals. A murine local lymph node assay(LLNA) has been developed as an alternative to guinea pig models for assessing the contact sensitization potential of chemical. This study was carried out to evaluate the skin sensitization potential for chemicals in Balb / c mice by LLNA. A murine local lymph node assay(LLNA) has been developed as an alternative to guinea pigs for contact sensitization potential. This study was carried out to investigate the skin sensitization potential of four allergenic chemicals, strong allergen, p-phenylenediamine (PPD), moderate allergen, cinnamaldehyde, and isoeugenol, and weak allergen, citral, by LLNA using a non-radioistopic endpoint. Female Balb/c mice were exposed topically to PPD, cinnamaldehyde, isoeugenol, and citral following LLNA protocol. Lymph node (LN) weight and cell proliferation in ears and auricular lymph node using bromode- oxyuridine (BrdU) immunohistochemistry were evaluated. Skin reactions, consisting of increased ear thickness and the presence of inflammatory infiltrates, were observed in mice treated with PPD, cinnamaldehyde, and isoeugenol compared to the vehicle control. The relative weight of the lymph nodes in the mice treated with four allergen were increased compared to vehicle control. The SI values in lymph nodes of the mice exposed to 10% PPD and 30% isoeugenol were increased more than threefold compared with that of the control. The SI values of the chemicals showed the comparative strength of proliferation in lymph node as following order of PPD>cinnamaldehyde>isoeugenol>citral. These results suggest that the measurement of the SI in lymph node using BrdU immunohistochemistry could provide a useful method to screen the allergenic potential of chemicals.
BrdU 면역조직화학염색을 이용한 국소 림프절 시험법(Local Lymph node assay) 연구
이종권 ( Jong Kwon Lee ),주정흔 ( Jung Hun Ju ),김정래 ( Jung Rae Kim ),손경희 ( Kyung Hee Sohn ),김진호 ( Jin Ho Kim ),김인영 ( In Young Kim ),전은영 ( Eun Young Jun ),김승희 ( Seung Hee Kim ),박귀례 ( Kui Lea Park ) 한국동물실험대체법학회 2007 동물실험대체법학회지 Vol.1 No.1
Allergic contact dermatitis (skin sensitization) may be caused by a wide variety of chemicals. A murine local lymph node assay(LLNA) has been developed as an alternative to guinea pig models for assessing the contact sensitization potential of chemical. This study was carried out to evaluate the skin sensitization potential for chemicals in Balb / c mice by LLNA. A murine local lymph node assay(LLNA) has been developed as an alternative to guinea pigs for contact sensitization potential. This study was carried out to investigate the skin sensitization potential of four allergenic chemicals, strong allergen, p-phenylenediamine (PPD), moderate allergen, cinnamaldehyde, and isoeugenol, and weak allergen, citral, by LLNA using a non-radioistopic endpoint. Female Balb/c mice were exposed topically to PPD, cinnamaldehyde, isoeugenol, and citral following LLNA protocol. Lymph node (LN) weight and cell proliferation in ears and auricular lymph node using bromode- oxyuridine (BrdU) immunohistochemistry were evaluated. Skin reactions, consisting of increased ear thickness and the presence of inflammatory infiltrates, were observed in mice treated with PPD, cinnamaldehyde, and isoeugenol compared to the vehicle control. The relative weight of the lymph nodes in the mice treated with four allergen were increased compared to vehicle control. The SI values in lymph nodes of the mice exposed to 10% PPD and 30% isoeugenol were increased more than threefold compared with that of the control. The SI values of the chemicals showed the comparative strength of proliferation in lymph node as following order of PPD>cinnamaldehyde>isoeugenol>citral. These results suggest that the measurement of the SI in lymph node using BrdU immunohistochemistry could provide a useful method to screen the allergenic potential of chemicals.