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주정흔 ( Jung Hun Ju ),임채형 ( Chae Hyung Lim ),김진호 ( Jin Ho Kim ),이정표 ( Jeong Pyo Lee ),손경희 ( Kyung Hee Son ),홍순근 ( Soon Keun Hong ),권태린 ( Tae Rin Kwon ),장미 ( Mi Chang ),김동섭 ( Dong Sup Kim ),윤혜성 ( Hae Seo 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1
Atopic dermatitis (AD) is a common pruritic disease and its incidence is increasing in infancy and childhood. AD is characterized by extreme pruritus and chronically relapsing inflammations and prevalence of AD has increased in recent years, but its exact etiology is unclear. Antigen-induced release of inflammatory mediators from mast cells causes the immediate symptoms of IgE-mediated allergic diseases, including allergic rhinitis, atopic dermatitis and atopic eczema. Mast cells rapidly release various allergic mediators, including histamine, cytokines that mediated acute and chronic allergic reaction. Since mast cells play a central role in the pathogenesis of various allergic diseases, we investigated the effect of exposure to 18 chemicals on the IgE-mediated allergic response in mast cells. We use two type of mast cells (RBL-2H3 cells, BMMC) to investigate the effects of chemicals on degranulation and cytokine production. Geraniol and formaldehyde significantly induced IgE-mediated mast cell degranulation. Dermatophagoides pteronyssinus allergen 1(DP) and dermatophagoides farinae allergen 1(DF) also induced degranulation without statistically significance. Also, we showed that release of IL-1β, IL-6 and GM-CSF was increased by treatment of geraniol. Also, We found that DP and DF induced release of IL-1β, IL-6, GM-CSF and TNF-α. These results suggest that in vitro method using mast cells degranulation and allergic cytokine secretion assay are useful for screening of atopic dermatitis trigger.
주정흔 ( Jung Hun Ju ),이용경 ( Yong Kyoung Lee ),손경희 ( Kyung Hee Son ),이정표 ( Jeong Pyo Lee ),김진호 ( Jin Ho Kim ),임채형 ( Chae Hyung Lim ),홍순근 ( Soon Keun Hong ),권태린 ( Tae Rin Kwon ),장미 ( Mi Chang ),김동섭 ( Dong 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1
Phototoxicity is an acute toxic response induced by skin irradiation after the systemic or local administration of a chemical and subsequent exposure to light. Thus, it is necessary to evaluate phototoxicity of chemicals or products that are applied to the skin. Although the previous studies on the assessment of phototoxicity have been done using animals, it is necessary to establish alternative test methods, as EU ban animal-tested cosmetic ingredients or products. In vitro 3T3 NRU PT is an alternative method for identification of phototoxicity hazard chemicals as it compares cytotoxicity between UV irradiation and non-irradiation groups. Accordingly the accumulated information of phototoxicity assessment and its data has been found to be insufficient in Korea. Thus, we tested ten phototoxic and three non-phototoxic chemicals, and assessed two UV filter ingredients using in vitro 3T3 NRU PT mehtod. As a result, we identified that this method can be used to predict phototoxicity of chemicals. However, Eusolex 9020, UVA filter ingredient, was misclassified in vitro as a false positive compared with in vivo result. It suggests that other adequate test methods should be accompanied in order to enhance accuracy of phototoxicity prediction.
연구논문 : 3T3 세포를 이용한 광독성 대체시험법 확립
주정흔 ( Jung Hun Ju ),이용경 ( Yong Kyoung Lee ),손경희 ( Kyung Hee Son ),이정표 ( Jeong Pyo Lee ),김진호 ( Jin Ho Kim ),임채형 ( Chae Hyung Lim ),홍순근 ( Soon Keun Hong ),권태린 ( Tae Rin Kwon ),장미 ( Mi Chang ),김동섭 ( Dong 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1
Phototoxicity is an acute toxic response induced by skin irradiation after the systemic or local administration of a chemical and subsequent exposure to light. Thus, it is necessary to evaluate phototoxicity of chemicals or products that are applied to the skin. Although the previous studies on the assessment of phototoxicity have been done using animals, it is necessary to establish alternative test methods, as EU ban animal-tested cosmetic ingredients or products. In vitro 3T3 NRU PT is an alternative method for identification of phototoxicity hazard chemicals as it compares cytotoxicity between UV irradiation and non-irradiation groups. Accordingly the accumulated information of phototoxicity assessment and its data has been found to be insufficient in Korea. Thus, we tested ten phototoxic and three non-phototoxic chemicals, and assessed two UV filter ingredients using in vitro 3T3 NRU PT mehtod. As a result, we identified that this method can be used to predict phototoxicity of chemicals. However, Eusolex 9020, UVA filter ingredient, was misclassified in vitro as a false positive compared with in vivo result. It suggests that other adequate test methods should be accompanied in order to enhance accuracy of phototoxicity prediction.