위상차 현미경 영상 내 푸리에 묘사자를 이용한 암세포 형태별 분류

강미선,이정엄,김혜련,김명희,Kang, Mi-Sun,Lee, Jeong-Eom,Kim, Hye-Ryun,Kim, Myoung-Hee 대한의용생체공학회 2012 의공학회지 Vol.33 No.4

Tumor cell morphology is closely related to its migratory behaviors. An active tumor cell has a highly irregular shape, whereas a spherical cell is inactive. Thus, quantitative analysis of cell features is crucial to determine tumor malignancy or to test the efficacy of anticancer treatment. We use 3D time-lapse phase-contrast microscopy to analyze single cell morphology because it enables to observe long-term activity of living cells without photobleaching and phototoxicity, which is common in other fluorescence-labeled microscopy. Despite this advantage, there are image-level drawbacks to phase-contrast microscopy, such as local light effect and contrast interference ring. Therefore, we first corrected for non-uniform illumination artifacts and then we use intensity distribution information to detect cell boundary. In phase contrast microscopy image, cell is normally appeared as dark region surrounded by bright halo ring. Due to halo artifact is minimal around the cell body and has non-symmetric diffusion pattern, we calculate cross sectional plane which intersects center of each cell and orthogonal to first principal axis. Then, we extract dark cell region by analyzing intensity profile curve considering local bright peak as halo area. Finally, we calculated the Fourier descriptor that morphological characteristics of cell to classify tumor cells into active and inactive groups. We validated classification accuracy by comparing our findings with manually obtained results.

장액성 삼출액의 도말 표본에서 반응성 중피세포와 샘암종 세포의 감별에 중피세포 표지자의 유용성

강미선,최수임,Kang, Mi-Sun,Choi, Soo-Im 대한세포병리학회 2006 대한세포병리학회지 Vol.17 No.2

Evaluation of serous effusions can include immunocytochemical stains that differentiate reactive mesothelial cell from adenocarcinoma cell. Among several positive mesothelial cell markers, we used desmin, CK5/6, WT1 and calretinin all known to have high sensitivity and specificity as selective mesothelial cell markers. We studied smears obtained with cytospin from 15 malignant and eight benign effusions. The mesothelial cells were positively stained by desmin, CK5/6, WT1 and calretinin in 60.9%, 29.1%, 26.7% and 56.5%, respectively among 8 benign and 15 malignant effusions; the adenocarcinoma cells were positively stained 6.7%, 13.3%, 1.0% and 0.0%, respectively among 15 malignant effusions. The percentage of positively stained mesothelial cells were somewhat lower for all antibodies compared to the results of previous studies. This was likely due to the differences in preparation methods and fixatives among studies. In conclusion, the use of desmin and calretinin were more valuable than CK5/6 and WT1 for distinguishing between reactive mesothelial cell and adenocarcinoma cells in serous effusion; however, choice of the proper preparation methods and fixatives are also important

한방병원 산후조리센터 내 신생아에 대한 연구

강미선,장규태,김장현,Kang Mi-Sun,Chang Gyu-Tae,Kim Jang-Hyun 대한한방소아과학회 2001 대한한방소아과학회지 Vol.15 No.2

We studied 100 neonates to enter a Dongguk University Oriental Hospital Postpartum Care Center, during 9 months from February 10. 2001 to November 4. 2001. The conclusion is following. 1. Newborn sex ratio was 1.70. 2. Delivery method : Normal vaginal delivery was 66 and cesarean section was 34. 3. Gestation age : Premature infants less than 37 weeks were 2 term infants from 37 to 41 weeks were 98 and post-term infants 42 or more weeks were zero. 4. Period between birth and admission : First to fifth day was 66 sixth to tenth day was 32 and eleventh or more day was 2. 5. Period of admission : 1 to 7 days were 8. 8 to 14 days were 65. 15 to 21 days were 23. and 22 to 28 days were 4. 6. Weight : Birth weight less than 2500g was 3. 2500 to 3999g was 92. and 4000g or more was 5. 7. Height : Zero week after birth the average height was 48.8cm, one week after birth was 50.0cm, two weeks after birth was 51.8cm, three weeks after birth was 53.2cm, four weeks after birth was 54.5cm. 8. Head circumference : Zero week after birth the average head circumference was 34.6cm, one week after birth was 35.3cm, two weeks after birth was 35.7cm, three weeks after birth was 36.3cm, four weeks after birth was 36.8cm. 9. Chest circumference : Zero week after birth the average chest circumference was 33.2cm, one week after birth was 33.5cm, two weeks after birth was 34.4cm, three weeks after birth was 35.1cm, four weeks after birth was 36.6cm. 10. Feeding : There was not breast feeding, artificial feeding was 8, and mixing feeding was 92. 11. Jaundice : Physiologic jaundice was 10, pathologic jaundice was 1.

소아 만성 재발성 호흡기 증상에 대한 고찰

강미선,김장현,Kang, Mi-Sun,Kim, Jang-Hyun 대한한방소아과학회 2002 대한한방소아과학회지 Vol.16 No.2

Respiratory tract symptoms such as cough, wheeze, and strider may occur frequently or persist for long periods in a substantial number of children, others may have persistent or recurring lung infiltrates with or without symptoms. It is important to study on clinical significance of this symptoms for avoiding unnecessary test or treatment. Chronic or Recurrent Respiratory Symptoms are similar to cold(感冒) and cough(咳嗽) in Oriental Medicine. Diagnostic criteria is followed : in case of children less than three years old, upper respiratory infections are more than seven per year or lower respiratory infections are more than three ; in case of children three to five years old, upper respiratory infections are more than six per year or lower respiratory infections are more than two ; in case of children six to twelve years old, upper respiratory infections are more than five per year or lower respiratory infections are more than two. In oriental medical treatment is classified into the treatment of internal use, external use, internal and external use, acupuncture, cupping therapy, and combination treatment of chinese and western medicine.

모(某) 한방병원(韓方病院) 소아과(小兒科)에 내원(來院)한 영아기(?兒期) 환아(患兒)에 대한 임상적(臨床的) 고찰(考察)

강미선,김장현,Kang, Mi-Sun,Kim, Jang-Hyun 대한한방소아과학회 2002 대한한방소아과학회지 Vol.16 No.2

The Purpose of this study was to investigate infancy patients who had visited Oriental Medicine Hospital, and so to consider a counterplan by oriental medicine. The study was composed of 101 new infancy patients who had visited Dongguk Kyeongju Oriental Medicine Hospital during 1 year from January 2001 to December 2001. The results were as follows : 1. Male children are 65(64.3%), female children are 36(35.6%), male to female ratio is 1.8: 1. 2. In age distribution, 1 month 5.9% ; 2 month 10.9%, 3 month 4.0%, 4 month 11.9%, 5 month 5.9%, 6 month 9.9%, 7 month 10.9%, 8 month 10.9%, 9 month 10.9%, 10 month 14.8%, 11 month 4.0%. 3. According to systematic division of the chief complaint, respiratory diseases are 37.6%, digestive diseases are 25.7%, nervous diseases are 21.8%, urogenital diseases are 1.0%, musculoskeletal diseases are 1.0%, dermatologic diseases are 7.9%, infirmity diseases are 3.0%. 4. In treatment, herb-medication is 86.1%, consultation is 7.9%, acupuncture is 17.8%, moxibution is 2.0%, venesection is 14.8%, aromatherapy is 4.9%, chimsband is 16.8%.

아버지 양육참여도와 유아의 자아탄력성 간의 관계에서 유아 행복감의 매개효과

강미선 ( Kang Mi Sun ),김혜순 ( Kim Hye Soon ) 미래유아교육학회 2017 미래유아교육학회지 Vol.24 No.4

본 연구의 목적은 아버지의 양육참여도와 유아의 자아탄력성 간의 관계에서 유아 행복감의 매개효과가 나타나는지를 규명하는 것이었다. 서울시에 위치한 유아교육기관에 재원중인 만 3세~5세 유아와 아버지 191쌍을 연구대상으로 하였다. 설문지로 유아의 자아탄력성과 행복감 그리고 아버지의 양육참여도가 평가되었다. 수집된 자료는 SPSS/WIN 20.0과 AMOS 20.0 통계 프로그램을 활용하여 신뢰도분석과 빈도분석, 기술통계분석, 상관분석, 경로분석 및 매개효과 검증을 위한 Sobel test가 이루어졌다. 본 연구의 결과는 다음과 같다. 첫째, 아버지의 양육 참여도와 유아의 행복감, 유아의 자아탄력성 간의 상관관계는 각각 정적 상관성이 나타났다. 둘째, 아버지의 양육참여도와 유아의 자아탄력성 간의 관계에서 유아 행복감의 완전 매개효과가 나타났다. 이어서 매개변수로서 유아의 행복감이 자아탄력성에 미치는 영향에 대한 시사점이 논의되었다. The purpose of this study was to examine the mediation effects of young children’s happiness on the relationships between paternal involvement and their young children’s ego-resilience. The participants of this study were 191 pairs fathers and their young children who attended kindergartens and day-care centers in Seoul. The collected data were analyzed using SPSS/WIN 20.0 and AMOS 20.0. For the data analysis, reliability analysis, frequency analysis, descriptive statistic analysis, correlation analysis, path analysis were conducted. The results of the study were as follows: First, there were significant positive correlations among paternal involvement, their children’s ego resilience and happiness respectively. Second, there was a significant mediation effect of young children’s happiness on the relationships between paternal involvement and their young children’s ego resilience. Additionally, several implications of mediation effects of young children’s happiness on the relationships between paternal involvement and their young children's ego-resilience were concerned.

<양문충의록> 연구 -이본비교를 중심으로-

강미선 ( Mi Sun Kang ) 한국고소설학회 2007 古小說 硏究 Vol.23 No.-

「文脈のモニタ-」をリソ-スとする實踐の構想

강미선 ( Mi Sun Kang ) 한국일본어교육학회 2015 日本語敎育 Vol.0 No.72

This study presented the practice design targeting the beginning Japanese class in the viewpoint of ‘the Japanese education to support a learner``s independent learning’. We suggested ``Monitor of Context`` as the resource of practice, and presented the design of the practice using the concept. The flow of practice is largely divided into 3 stages. 1) The stage of consciousness raising that the grammar ability as knowledge is not connected to language application as it is, 2) The introduction to ‘Monitor of Context’ and the explanation about composition task, 3) The classroom activity with the resource of ‘Monitor of Context’. This study presented the practice design based on the educational factors such as a teacher``s educational viewpoint and educational belief, and educational contents and showed the possibility of education to develop ``learning power`` in the learner.

쥐 해마의 유전자 발현 그리드 데이터를 이용한 특징기반 유전자 분류 및 영역 군집화

강미선(Mi-Sun Kang),김혜련(HyeRyun Kim),이석찬(Sukchan Lee),김명희(Myoung-Hee Kim) 한국정보과학회 2016 정보과학회논문지 Vol.43 No.1

뇌의 유전자 발현 정보는 영역별 기능과 밀접한 관련이 있어 이를 분석하기 위해 다수의 유전자들 간의 발현 정도 및 발현 위치 정보와의 관계에 대한 연구가 이루어지고 있다. 본 논문에서는 컴퓨터 기술을 통해 알렌 뇌과학연구소에서 제공하는 약 2만여개의 쥐 뇌 유전자 발현 정보 중 뇌의 해마 영역을 중점적으로 분석하여 유전자들을 자동으로 분류해내고 발현 위치 정보를 기반으로 군집화하여 가시화하는 방법을 제안한다. 이를 통해 해마 내 전체적으로 발현되는 유전자들과 특정 영역에만 발현되는 유전자들을 분류할 수 있었고 그 중 특정 영역에 발현되는 유전자들의 위치정보 기반으로 군집화된 데이터를 뇌 지도와 함께 관찰 할 수 있었다. 본 연구는 뇌의 기능과 영역과의 관계성 관련 생물학적 연구를 위한 실험군 선정작업에 이용되어 실험설계시간을 줄일 수 있고 기존에 알려진 뇌의 해부학적 구조보다 더욱 세분화된 구조를 발견할 수 있는 가능성을 제시할 것으로 기대된다. Brain gene expression information is closely related to the structural and functional characteristics of the brain. Thus, extensive research has been carried out on the relationship between gene expression patterns and the brain’s structural organization. In this study, Principal Component Analysis was used to extract features of gene expression patterns, and genes were automatically classified by spatial distribution. Voxels were then clustered with classified specific region expressed genes. Finally, we visualized the clustering results for mouse hippocampal region gene expression with the Allen Brain Atlas. This experiment allowed us to classify the region-specific gene expression of the mouse hippocampal region and provided visualization of clustering results and a brain atlas in an integrated manner. This study has the potential to allow neuroscientists to search for experimental groups of genes more quickly and design an effective test according to the new form of data. It is also expected that it will enable the discovery of a more specific sub-region beyond the current known anatomical regions of the brain.

대중 춤 매니아의 사회문화적 특성

강미선(Mi Sun Kang) 한국스포츠사회학회 2002 한국스포츠사회학회지 Vol.15 No.2