쌀 및 밀 복합분의 물리적 성질 및 제빵시험

이춘영,김성곤,피이마스톤,Lee, Chun-Yung,Kim, Sung-Kon,Marston, P.E. 한국식품과학회 1979 한국식품과학회지 Vol.11 No.2

Rheolegical and baking properties of blends containing 10, 20 and 30 % of rice flours (Milyang 23, non-waxy and Tongil waxy) with wheat flour were investigated. Milyang-wheat blends showed higher amylograph paste viscosities at all reference points than waxy-wheat blends. Rice-wheat four blends had shorter farinograph stability than f·heat flour; however, the dough development time was similar between two blends. Breads produced from either Milyang-wheat or waxy-wheat flour blends at 10% rice level were acceptable compared with breads produced from wheat flour. 밀가루에 멥쌀(밀양 23호) 또는 통일찹쌀 가루를 10, 20 및 30% 대체한 복합분의 물리적 성질 및 제빵 적성을 검토하였다. 밀-밀양 복합분은 밀-찹쌀 복합분 보다 높은 점도를 보였다. 복합분의 파리노그라프 안정성은 밀가루 보다 짧았으나 최적 반죽발전 시간은 비슷하였다. 제빵 적성은 밀-밀양 복합분이 다소 좋은 결과를 보였고 대체로 밀가루(멥쌀 또는 찹쌀)를 20%수준까지는 대체할 수 있을 것으로 보인다. 10%수준으로는 밀가루 빵에 크게 손색이 없는 제품이 가능하였다.

녹두 ( Phaseolus aureus ) 와 팥 ( Phaseolus angularis ) 의 저장단백질의 분리와 그 특성에 관하여

이춘영,김수언,유기중 ( Chun Yung Lee,Soo Un Kim,Ki Jung Yoo ) 생화학분자생물학회 1978 BMB Reports Vol.11 No.3

A successful fractionation of the storage proteins in mung bean (P. aureus) and small red bean (P. angularis) seeds with chromatography on Sephadex G-150 was attained. Isolated proteins, tentatively designated as F2 globulin and F1 globulin, were classified as vicilin and presumably as legumin, respectively. Disc-PAGE of the major storage protein, F2 globulin, displayed a diffused band stained with periodic acid-Schiff`s base reagent. However, SDS-PAGE demonstrated the faint bands besides five major bands:.Apparent molecular weights of each subunit determined with SDS-PAGE were 64,000 53,000. 50,000. 27,000 and 23,400 in F2 globulin of the mung bean and 55,300. 53,000 29,600 26,600 and 25,000 in small red bean.

녹두(Phaseolus aureus)와 팥(Phaseolus angularis)의 저장단백질의 분리와 그 특성에 관하여

이춘영,김수언,유기중,Lee, Chun-Yung,Kim, Soo-Un,Yoo, Ki-Jung 생화학분자생물학회 1978 한국생화학회지 Vol.11 No.3

녹두(P.aureus)와 팥(P.angularis)의 저장단백질을 Sephadex G-150으로 크로마토그라피하여 분리하고 그의 전기영동상 특성을 연구하였다. 분리된 단백질은 잠정적으로 Fl globulin 과 F2 globulin 으로 명명하였는데 각각 legumin과 vicilin으로 동정되었다. F2 globulin은 주 저장단백질로서 당단백질이었으며 disc-전기영동에서 확산된 하나의 분리대로 나타났다. 한편 SDS-전기영동과 PAW-전기영동에서는 작은 분리대 몇개가 주분리대와 함께 나타났다. SDS-전기영동에서 나타난 다섯 주분리대의 겉보기 분자량은 녹두에서 64,000, 53,000, 50,000, 27,000 그러고 23,400 이었으며 팥에서는 55,300, 53,000, 29,600, 26,600 그리고 25,000 이었다. A successful fractionation of the storage proteins in mung bean (P. aureus) and small red bean (P. angularis) seeds with chromatography on Sephadex G-150 was attained. Isolated proteins, tentatively designated as F2 globulin and F1 globulin, were classified as vicilin and presumably as legumin, respectively. Disc-PAGE of the major storage protein, F2 globulin, displayed a diffused band stained with periodic acid-Schiff's base reagent. However, SDS-PAGE demonstrated the faint bands besides five major bands. Apparent molecular weights of each subunit determined with SDS-PAGE were 64,000, 53,000, 50,000, 27,000 and 23,400 in F2 globulin of the mung bean and 55,300, 53,000 29,600, 26,600 and 25,000 in small red bean.

이은 교환수지에 의한 쌀 (八達) 의 아미노산정량에 관하여

이춘영(Chun Yung Lee),이태영(Tae Yung Lee),권태완(Tai Wan Kwon) 한국응용생명화학회(구 한국농화학회) 1961 Applied Biological Chemistry (Appl Biol Chem) Vol.2 No.1

대두 Trypsin Inhibitor의 간이검정법

조구형,이춘영,홍종욱,김인수,Jo, Ku-Hyung,Lee, Chun-Yung,Hong, Jong-Uek,Kim, In-Soo Korean Society of Food Science and Technology 1986 한국식품과학회지 Vol.18 No.5

The specific reaction of trypsin inhibitors with trypsin to form stable complexes was successfully applied for detection of trypsin inhibitors in soybean. Soybean extract was treated with $Ca^{++}$ to remove globulin fraction, followed by digestion with trypsin and fractionated by chromatography on Sephadex G-50. The void volume fraction contained the trypsin-trypsin inhibitor complexes as well as trypsin. The trypsin inhibitors were then detected by their molecular weight differences on SDS-polyacrylamide gel electrophoresis, in which the complexes dissociate into trypsin and its inhibitors. With the method proposed, trypsin inhibitors were indentified by their ability forming the stable complexes with trypsin and their anti-tryptic moiety. The formation of the complexes with trypsin was further confirmed by two dimensional electrophoresis and DEAE-Sephadex A-25 chromatography. Employing the proposed method, it was found that soybean (Glycine max cv. Hill) contained 7 trypsin inhibitors. Trypsin inhibitor가 trypsin과 안정한 복합체를 형성하고 동시에 일반 단백질은 trypsin에 의하여 가수분해 되는 원리를 이용하여 trypsin inhibitor를 용이하게 검정 할 수 있는 방법을 고안하였다. trypsin으로 가수분해 시킨 대두추출액을 Sephadex G-50을 이용하여 trypsin-trypsin inhibitor 복합체를 분리시킨 후에 SDS 전기 영동으로 trypsin inhibitor를 복합체를 분리시킨 후에 SDS 전기 영동으로 trypsin inhibitor를 검정할 수 있었다. 이들 trypsin inhibitor는 trypsin에 의한 2차 가수분해에서도 가수분해 되지 않았으며, 또한 2차원 전기영동과 DEAE-Sephades A-25크로마토 그래피를 이용하여 trypsin inhibitor가 trypsin과 복합체를 형성하는 능력을 검정함으로써 본 방법의 유효성을 확인하였다. 본 실험 방법으로 대두(Hill 품종)의 trypsin inhibitor를 검정한 결과 7개의 trypsin inhibitor를 찾아 낼 수 있었다.

대두(大豆)(Glycine max)의 ${\gamma}-Conglycinin$의 정제(精製)와 특성(特性)에 관(關)하여

김인수,이춘영,Kim, In-Soo,Lee, Chun-Yung 한국응용생명화학회 1979 Applied Biological Chemistry (Appl Biol Chem) Vol.22 No.1

대두(大豆)의 ${\gamma}-conglycinin$을 정제(精製)하고 그의 물리일(物理一) 화학적(化學的) 성질(性質)을 연구(硏究)하여 다음의 결과(結果)를 얻었다. Gamma-conglycinin은 대두(大豆)의 pH 4.5, 0.2M Nacl 추출액을 Sephadex G-150으로 분리하여 얻은 7S globulin을 DEAE-Sephadex A-50으로 column chromatography하여 정제(精製)하였다. 정제된 ${\gamma}-conglycinin$은 면역전기영동(免疫電氣泳動), polyacrylamide gel 전기영동(電氣泳動)과 gel isoelectric focusing 상으로 순수(純粹)하였다. 등전점(等電點)은 pH 5.4이었으며 질소(窒素) 16.21%, mannose 4.18, glucosamine 1.21%의 함량(含量)이었다. 아미노산 조성(組成)은 일반적(一般的)으로 lysine, dicarboxylic acid와 암모니아태(態) 질소(窒素)의 함량(含量)이 높았고 함황(含黃)아미노산과 tryptophane의 함량(含量)은 낮은 편이었다. Subunit의 등전점(等電點)은 pH $4.5{\sim}5.5$에 분희(分希)하였는데 그중 pH $4.6{\sim}5.0$에 위치(位置)한 것은 분자량(分子量) 38,000의 당(糖) peptide이었으며 pH $5.0{\sim}5.5$의 것은 분자량(分子量) 32,000의 단순(單純) peptide이었다. The physical and chemical properties of ${\gamma}-conglycinin$ of soybean (Glycine max) were investigated. The soybean protein extracted from soybean meal using 0.2M NaCl solution at pH 4.5 was passed through a Sephadex G-150 column to isolate 7S globulin. ${\gamma}$-Conglycinin was isolated and purified from the 7S globulin with a DEAE Sephadex A-50 column chromatography. The protein preparation was pure on immunoelectrophoresis, polyacrylamide gel electrophoresis and gel isoelectric fouling. It had an isoelectric point at pH 5.4 and contained 16.12% nitrogen, 4.18% mannose and 1.21% glucosamine. Amino acid composition, in general, shaved that ${\gamma}-conglycinin$ contained higher contents of lysine, dicarboxylic acids and ammonia nitrogen, and lower contents of sulfur-containing amino acids and tryptophan. The subunits of ${\gamma}-conglycinin$ were distributed in the range of pH 4.6-5.5. The subunits located in the pH region of 4.6-5.0 and 5.0-5.5 were glycopeptides (molecular weight of 38,000) and simple peptide (MW of 32,000), respectively.

$Cs^{137}$의 迅速定量法

김태순,박태원,이춘영,서경숙,Kim, Tai-Soon,Park, Tai-Won,Lee, Chun-Yung,Seu, Kyung-Sook 대한화학회 1961 대한화학회지 Vol.5 No.1

Two methods for the determination of Cesium-137 in various foods(ashes) were compared. The one comprises two steps, the first being the separation of alkalies as cobaltinitrites or perchlorates by the radiochemical method, and the second being the measurement of photopeak area due to the presence of Cs-137 by the use of scintillation spectrometer. The other method is the direct subjection of ash samples to the spectrometry without the radiochemical separation. The combined contribution of K-40 and Rb-87 to the spectrum was not found on account of the difference in energies of these nuclides. These two methods gave, a good agreement in the determination of Cs-137 in 50-100g ash samples of vegetables or cereals under investigation.

작물의 유황영양에 관한 연구( I ) -포장재배수도의 유황영양실태-

박훈,김영섭,이춘영,Park, Hoon,Kim, Yong-Sup,Lee, Chun-Yung 한국응용생명화학회 1971 Applied Biological Chemistry (Appl Biol Chem) Vol.14 No.2

기후 토양 시비조건이 다른 수개포장에서 자란 벼의 유황영양상태를 조사한 결과 아래와 같다. 1. 이앙후 30 일경 유황결핍포장의 수도체내 유황의 임계함량(critical percentage)은 0.2%이상인것 같다. 2. 수확기 S 함량평균치는 이삭과 짚에서 각각 0.22% 및 0.43%이고 유안시비는 짚에서의 농도를 더욱 높였다. 3. 볏짚과 이삭의 S 함량비는 유안시비구에서만 위도가 낮을수록 증가하는 경향이나 이삭에의 전이율은 이와 관계없고 시비구에서 평균 26% 무비구에서 29%였다. 4. 유황흡수량은 무비구에서 6kg/10a 시비구에서 10 kg이었다. 5. 부위별 유량함량은 엽신+엽초>간>이삭의 순서이며 어느부위서나 저수답벼에서 높았다. The nutritional status of sulfur in rice plants grown on various field conditions different in soil fertility, fertilizers used and weather was investigated as follows. 1. The critical percentage of sulfur in rice shoot grown in a sulfur deficient field appears to be over 0.2 around 30 days after transplanting. 2. The mean sulfur contents at harvesting stage were 0.22% and 0.43% in the ear and straw, respectively and increased more in the straw by the application of ammonium sulfate. 3. The ratio of sulfur contents in the straw and ear (straw/ear) showed the increasing tendency from north to south only in the fertilized fields. But it was not as such in the percent translocation, the mean values of which were 26% and 29% in the fertilized and non-fertilized, respectively. 4. The sulfur uptake amounted to 6kg and 10kg per acre in the non-fertilized and fertilized, respectively. 5. The sulfur content was decreased in the order of leaf blade+leaf sheath, stem and ear, and was higher in the low productive field than that of high productive field.

염색법에 의한 쌀 단백질의 함량 및 질의 평가에 관한 연구

김성곤(Seung Kon Kim),이춘영(Chun Yung Lee),박훈(Hoon Park) 한국식품과학회 1971 한국식품과학회지 Vol.3 No.2

Antilipolytic Activity를 보유하는 인삼 Oligopeptide의 추출 및 정제

김수일,나지영,조도현,이춘영,Kim, Su-Ill,Na, Jee-Yeong,Jo, Do-Hyun,Lee, Chun-Yung 한국응용생명화학회 1987 한국농화학회지 Vol.30 No.1

To investigate ginseng oligopeptides with biological activities, the water extract was purified by ultra-filtration, gel filtration, ion-exchange and thin layer chromatography. Ultra-filtered water extract exhibited antilipolytic activity, inhibiting epinephrine-induced lipolysis in the isolated fat cells of rat epididymal adipose tissue. The filtrate was separated into 3 fractions by Sephadex G-25 gel filtration. Peptides were found only in the first fraction(S-FI). Saponine and sugars were also detected in tie fraction. S-FI fraction resolved further into 6 fractions by Dowex 50 ion-exchange chromatography. The sugar and saponine depleted fraction(P-F2) from the second chromatography showed antilipolytic activity. The P-F2 fraction revealed 6 spots on TLC. The 6 spots were isolated by TLC and identified as peptides. 인삼 성분중 생리적 활성을 가지고 있는 oligopeptide를 검출하고 분리하기 위하여 물 추출액을 ultra-filtration 한 후 gel filtration, ion-exchange chromatography 및 thin layer chromatography 등을 행하였다. 인삼 물 추출액으로부터 고분자 물질을 제거한 ultra-filtrate는 epinephrine에 의해 유도된 fat cell의 lipolysis를 저해하는 antilipolytic activity를 보유하고 있었으며 Sephadex G-25 gel filtration에 의해 3개 fraction으로 나뉘어졌다. 이중 첫번째 fraction(S-FI)만이 peptide 성분을 함유하고 있었으며 saponin 및 당도 검출되었다. S-FI fraction은 $Dowex\;50{\times}2\;ion-exchange\;chromatography$에 의하여 6개의 $fraction(P-F1{\sim}P-F6)$으로 분리되었고 TLC검정 결과 P-F2 fraction이 peptide fraction으로 antilipolytic를 보유하고 있었으며 TLC로 분리한 6개 spot는 각각 가수 분해한 전후의 TLC pattern을 비교해 본 결과 모두 oligopeptide임이 밝혀졌다. S-FI fraction에 존재한 saponine과 당은 P-F1 fraction 에서 모두 용출되었다.