LPS로 유도된 RAW 264.7세포에 대한 벼메뚜기(Oxya chinensis sinuosa) 에탄올 추출물의 항염증 효과

윤영일(Young-Il Yoon),정미연(Mi Yeon Chung),황재삼(Jae-Sam Hwang),구태원(Tae-Won Goo),안미영(Mi-Young Ahn),이영보(Young-Bo Lee),한명세(Myung-Sea Han),윤은영(Eun-Young Yun) 한국생명과학회 2014 생명과학회지 Vol.24 No.4

본 연구에서는 벼메뚜기 에탄올 추출물의 항염증 효능을 분석하기 위해 LPS로 염증 유도된 RAW 264.7 세포를 이용하였다. OCE의 항염증 효능을 확인 하기 위해서, 염증 유도된 RAW 264.7 세포에 대해 OCE 농도 의존적으로 염증성 사이토카인인 TNF-α와 IL-6의 유전자발현 및 단백질 생성을 감소시킴을 real-time PCR과 ELISA로 확인하였다. 또한, NF-κB p65의 핵으로 이동이 차단됨을 면역형광염색으로 확인하였으며, iNOS와 COX-2 단백질 발현을 감소시키는 것을 Western blot 분석으로 확인하였다. 이상의 연구결과를 통해 벼메뚜기는 염증에 의한 NF-κB p65의 활성과 TNF-α와 IL-6의 생성과 iNOS 및 COX-2의 발현을 억제하는 항염증 효능을 갖고 있는 것을 확인하였다. Although the grasshopper Oxya chinensis sinuosa has long been used as food in Korea, there is little data on its functional effects. In this study, we investigated the anti-inflammatory effect of O. c. sinuosa ethanol extract (OCE) in RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for induction of inflammation. First, we determined that there is no cytotoxicity at 2,000 μg/ml or less of OCE in RAW 264.7 cells. To evaluate the anti-inflammatory effects of OCE, we investigated expression levels of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, and pro-inflammatory enzymes such as inducible nitric oxide synthase (iNOS) and cyclo-oxygenase- 2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether OCE could inhibit translocation of NF-κB p65 into the nucleus in LPS induced RAW 264.7 cells. As a result, we found that the mRNA and protein levels of TNF-α and IL-6 decreased in LPS-induced RAW 264.7 cells after treatment with OCE in a dose-dependent manner. In addition, we confirmed a 2,000 ug/ml concentration of OCE inhibited translocation of NF-κB p65 by immunnostaining and Western blot analysis, and a decrease in the protein expression levels of iNOS and COX-2. Accordingly, we suppose that OCE has an anti-inflammatory effect through down-regulation of TNF-α, IL-6, iNOS, and COX-2 related to NF-κB p65 inflammatory signaling pathways.

LPS로 염증 유도된 RAW 264.7세포에 대한 참콩풍뎅이(Popillia flavosellata) 에탄올 추출물의 항염증 효과

윤영일(Young-Il Yoon),황재삼(Jae-Sam Hwang),김미애(Mi-Ae Kim),안미영(Mi Young Ahn),이영보(Young-Bo Lee),한명세(Myung Sae Han),구태원(Tae-Won Goo),윤은영(Eun-Young Yun) 한국생명과학회 2015 생명과학회지 Vol.25 No.9

본 연구에서는 참콩풍뎅이(Popillia flavosellata) 에탄올 추출물(PFE)의 항염증 효능을 분석하기 위해 PFE를 농도별(500, 1,000, 2,000 ㎍/ml)로 대식세포인 RAW 264.7에 처리 시 최고 처리농도인 2,000 ㎍/ml까지 통계적인 유의성 있는 독성이 없음을 확인하였다. LPS (100 ng/ml)로 염증 유도된 RAW 264.7 세포에 PFE를 농도별(500, 1,000, 2,000 ㎍/ml)로 동시 처리 시 농도 의존적으로 염증성사이토카인인 TNF-α와 IL-6의 단백질 생성을 통계적인 유의성(p<0.001)있게 억제함을 확인하였다. 또한 염증 유도된 RAW 264.7 세포에 PFE 동시 처리 시 NF-κB p65의 핵으로 이동이 차단됨과 iNOS와 COX-2의 단백질 발현을 감소시키는 것을 확인하였다. 이상의 연구결과를 통해 참콩풍뎅이는 염증에 의해 활성화된 TLR-4 신호전달과정을 조절하는 NF-κB p65의 활성과 염증성사이토카인 TNF-α와 IL-6의 생성 및 염증성효소 iNOS와 COX-2의 생성을 억제하는 항염증 효능이 있음을 확인하였다. The beetle Popillia flavosellata has been no reported its functional effects. In this study, we investigated the anti-inflammatory effect of P. flavosellata ethanol extract (PFE) on RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for the induction of inflammation. First, we examined the cytotoxicity of PFE in the RAW 264.7 cells at a concentration of 2,000 μg/ml or less. To evaluate the anti-inflammatory effects of PFE, we investigated the expression levels of proinflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, and proinflammatory enzymes, such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether PFE inhibited the translocation of nuclear factor kappa B (NF-κB) p65 into the nucleus in the LPS-induced RAW 264.7 cells. We found that the protein levels of TNF-α and IL-6 were decreased in the LPS-induced RAW 264.7 cells after the treatment with PFE in a dose-dependent manner. In addition, we confirmed that PFE inhibited the translocation of NF-κB p65 into the nucleus, as well as the protein expression levels of iNOS and COX-2. Accordingly, we propose that PFE exerts an anti-inflammatory effect through the down-regulation of NF-κB p65, TNF-α, IL-6, iNOS, and COX-2 via the toll like receptor (TLR)-4 inflammatory signaling pathway.

동계 이중외피와 내부식재에 의한 실내 온도 특성에 관한 연구

윤영일(Yun, Young-Il),조주영(Cho, Ju-Young) 한국생태환경건축학회 2015 한국생태환경건축학회 논문집 Vol.15 No.5

요양시설의 유용조도 분석에 관한 연구 -기상데이터 기반 동적 자연채광 시뮬레이션을 기반으로-

윤영일 ( Young Il Yun ),조주영 ( Ju Young Cho ),이효원 ( Hyo Won Lee ) 한국의료복지건축학회 2011 의료·복지 건축 Vol.17 No.1

Daylight is highly beneficial for improving the indoor environmental quality and reducing building energy consumption, daylighting applications are scarcely considered, especially during the care facility design process, because of lack of previous studies on elderly- light environment and complex simulation process. Therefore, daylighting process were performed using ECOTECT, which has various advantage such as easy user interface and simple simulation processes. Moreover, dynamic daylight simulation were performed using whether data. Static simulation are performed to compute static metrics such as daylight factor, whereas dynamic simulation are performed for dynamic metrics such as daylight autonomy and useful daylight illuminance using annual weather data On the basis of daylight autonomy and useful daylight illuminance analysis result, variations in annual daylight performances. A parametric and regression analysis of the window-to-wall ratio and visible transmittance showed that daylight factor, daylight autonomy increased with window-to-wall ratio and visible transmittance. It can be concluded that this new daylight criteria. useful daylight illuminance, will enable architect to obtain better fenestration design.

농어촌주택 표준설계의 유용조도 분석에 관한 연구

윤영일(Yun, Young Il),송정석(Song, Jeong Suk),이효원(Lee, Hyo Won) 한국생태환경건축학회 2011 한국생태환경건축학회 논문집 Vol.11 No.1

Primary Cultures of Drosophila melanogaster Gut Cells for Studies of Intestinal Stem Cell Regulation

Young-Il Yoon(윤영일),Jae-Sam Hwang(황재삼),Tae-Won Goo(구태원),Myung-Sae Han(한명세),Mi-Young Ahn(안미영),Eun-Young Yun(윤은영) 한국생명과학회 2012 생명과학회지 Vol.22 No.5

초파리는 발생과 질병연구를 위한 모델 곤충으로 널리 이용되어 왔다. 본 연구에서도 초파리를 모델곤충으로 한 장질환 연구의 일환으로 다양한 병원균 감염 및 장질환 유발시 어떻게 장줄기세포가 작용하는지를 이해하기 위해 초파리 장세포의 일차배양 방법을 확립하였다. 초파리 성충으로부터 장을 해부하고 다양한 효소를 처리하여 장세포를 분리한 후 배양하였다. 배양세포의 생존여부는 현미경 검경 및 MTS assay에 의해 확인한 결과 배양 후 9일째 최대 증식되었고 14일까지 생존함을 확인할 수 있었다. 또한 장줄기세포 및 장내분비세포의 존재도 immunostaining에 의해 확인하였다. 따라서 본 연구에서 구축된 초파리 일차배양 장세포는 다양한 유전자에 의한 장줄기 세포 조절연구뿐만 아니라 장에서 발생하는 다양한 질병을 연구하는 도구로 매우 유용할 것으로 추측된다. Drosophila melanogaster has been used as a useful model to study development and disease. In this study, we established the primary culture method of Drosophila in the intestine to understand how intestinal stem cells (ISCs) mediate tissue repair during infection and disease. To obtain intestinal cells, we separated intestines from adult flies and isolated single cells by enzymatic treatment. The survival of cultured cells was measured using MTS-analysis. The maximum growth rate of the cells was observed on the 9th day after seeding. In addition, the presence of ISCs and enteroendocrine cells was confirmed by delta and prospero staining. Accordingly, we supposed that Drosophila melanogaster gut cells established in this study are probably useful in studies about intestinal stem cell regulation and various diseases occurring in the intestine.

조리방법별 갈색거저리 유충의 물리적 및 관능적 특성

백민희,윤영일,김미애,황재삼,구태원,윤은영,Baek, Minhee,Yoon, Young-Il,Kim, Mi Ae,Hwang, Jae-Sam,Goo, Tae-Won,Yun, Eun-Young 한국식품조리과학회 2015 한국식품조리과학회지 Vol.31 No.5

Recently, the Tenebrio molitor larva was recognized as a novel food ingredient by the Ministry of Food and Drug Safety in Korea. Accordingly, we investigated its physical and sensory characteristics to establish the cooking conditions that may increase the demand of T. molitor larvae as a food. In this study, T. molitor larvae were cooked by various methods such as hot air dry, oven-broil, roast, pan fry, deep fry, boil, steam, and by microwave. In the physical evaluation of texture, the hardness and fracturability values were highest when larvae were cooked in the microwave. The adhesiveness, springiness, and chewiness values were highest when larvae were boiled. Boiled and steamed larvae had the highest lightness (L value), while oven-broiled larvae had the highest redness (a value) and yellowness (b value) values. Sensory evaluations assessed the appearance, aroma, flavor, and texture of cooked T. molitor larvae. Steamed and boiled larvae sizes were significantly large and the form was well preserved similar to fresh larvae. The moisture heat cooked (steamed and boiled) T. molitor larvae had the aroma and flavor of steamed corn, canned pupa, and boiled mushroom. In case of oven-broiled T. molitor larvae, the aroma and flavor of mealworm oil, seafood, sweet and roasted sesame were higher than in those cooked by other methods. In texture among sensory evaluation, the hardness and crispiness were the highest in the hot air dried and oven-broiled larvae, whereas juiciness was significantly higher in the boiled and steamed. Accordingly, we suggest that oven-broiled T. molitor larva will be prefered by consumer, due to its the rich aroma and flavor.

흰점박이꽃무지(Protaetia brevitarsis)의 식품원료화를 위한 전처리 조건 확립

권은영(Eun-Young Kwon),유정미(Jeongmi Yoo),윤영일(Young-Il Yoon),황재삼(Jae-Sam Hwang),구태원(Tae-Won Goo),김미애(Mi-Ae Kim),최영철(Young-Cheol Choi),윤은영(Eun-Young Yun) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.3

본 연구에서는 흰점박이꽃무지를 식품원료화 하기 위한 전처리 조건을 설정하기 위해 세포독성, 이취감소 및 살균조건을 확립하였다. 유충에 살균 후 공급되는 세 가지 사료(참나무톱밥, 볏짚, 목초) 중에서 참나무톱밥을 공급하여 사육한 유충의 분말이 세포독성이 없었고, 유충의 발육단계를 크게 3령 유충, 용화직전 번데기, 번데기로 구분 지은 후 각각의 분말이 가지는 세포독성 유무를 확인한 결과에서 세포독성은 나타나지 않았다. 세포독성 검사 결과를 토대로 식품원료화하기 위한 유충의 표준사료는 세포독성이 전혀 나타나지 않고 전국 사육농가 중 가장 많이 이용하고 있는 발효 참나무 톱밥을 표준사료로 선정하였다. 흰점박이꽃무지의 분말전처리 조건으로 증기로 5분 동안 살균처리를 통해 사료 내의 미생물을 제거한 참나무톱밥을 급여하고 3일간 절식상태로 배변유도 후, 중장에 존재하는 내용물의 잔여도가 현저히 감소됨을 확인하였다. 또한 관능평가를 통해 3일간의 배변된 유충의 장 내용물의 잔여물이 감소함으로써 유충이 가지는 고유한 이취와 색깔이 눈에 띄게 개선되는 효과를 확인할 수 있었다. 배변 유도된 유충을 115℃, 0.9 kgf/cm³의 고온고압멸균을 5분간 실시한 후 동결건조 한 분말에서 세균 및 진균을 포함하는 미생물이 완전히 제거됨으로서 식품원료로서의 안전성을 확보할 수 있었다. The pharmacological efficacy of Protaetia (P.) brevitarsis larvae has been described in the Dongui Bogam. It is believed that the larvae are particularly useful for hepatic disorders. However, natural aversion has made it difficult to consume these larvae as food. Thus, we sought to make an eatable form of the larvae by establishing optimal conditions for larvae preparation. Larvae were selectively bred, sterilized, and a powder of larvae generated by freeze-drying. Afterward, the CellTiter 96<SUP>®</SUP> AQueous Non-Radioactive Cell Proliferation Assay (MTS) with the RAW 264.7 cell line was used to validate the safety of the powder as a food ingredient. We determined that oak sawdust sterilized by water vapor for 5 minutes could be used for larvae feed, and a feeding for 3~5 days followed by a fasting for 3 days were optimal conditions for larvae preparation. In addition, sterilization of larvae at 115oC and 0.9 kgf/cm³ (to avoid contamination of pathogenic bacteria and fungi) was successfully applied in the production of edible powder from P. brevitarsis. The optimized processes established in our experiments can be used in the industrial production of P. brevitarsis as a food ingredient.

생태마을 초기계획과 마을현황에 대한 비교 연구

송정석(Song, Jeong-Seok),윤영일(Yun, Young-Il),이효원(Lee, Hyo-Won) 한국생태환경건축학회 2008 한국생태환경건축학회 논문집 Vol.8 No.1

치유환경을 위한 광선반 부착방법에 따른 노인요양시설 침실 내 자연채광 유입 환경 연구

조주영(Cho, Ju-Young),이기호(Lee, Ki-Ho),윤영일(Yun, Young-Il),이효원(Lee, Hyo-Won) 한국생태환경건축학회 2011 한국생태환경건축학회 논문집 Vol.11 No.6