누에 품종에 따른 눈꽃동충하초 배양 특성

조유영 ( You Young Jo ),권해용 ( Haeyong Kweon ),이광길 ( Kwang Gill Lee ),김현복 ( Hyun Bok Kim ),김기영 ( Kee Young Kim ) 한국잠사학회 2015 한국잠사곤충학회지 Vol.53 No.2

동충하초는 곤충의 몸속에 들어가 버섯이 발생하는 곤충 기생성 약용 버섯으로 세계적으로 인체에 유용한 활성물질이 확인되었으며, 이를 누에를 이용하여 인공재배할 수 있는 기술이 개발되어 활용되고 있다. 그러나 누에 품종에 따른 동충하초의 생산성에 대한 연구 보고는 없는 실정이다. 본 연구에서는 장려품종 3품종(백옥잠, 금옥잠, 대성잠)과 특수품종 2품종(연록잠, 골든실크)을 이용하여 눈꽃동충하초의 재배조건과 특징을 조사하였다. 눈꽃동충하초 균주(P. tenuipes)에 대한 감염율은 금옥잠이 가장 높았으며, 백옥잠, 대성잠, 골든실크, 연록잠 순으로 감염율이 높았다. 눈꽃동충하초를 재배하기 위한 최적조건은 어두운 장소에서 번데기 당 약 4 cm2의 재배밀도로 22oC ± 1를 유지해 주는 것이었다. 또한 우리는 농가소득 향상을 위해, 동충하초의 수요가 있을 때 마다 수시로 재배할 수 있는 연중재배 기술을 개발하였다. 연중재배 방법은 먼저 동충하초균에 감염된 번데기 체내의 수분함량을 자연건조로 3% 이하로 낮추고, 이를 진공포장 후 4oC 이하에서 보관하는 3단계의 과정이 필수적이다. 이렇게 저온에 보관되어 있는 감염번데기는 언제든지 물에 침지하여 번데기 내의 함수율을 원래대로 복원 시킨 다음 최적 재배조건에서 재배하여 동충하초를 생산할 수 있게 함으로 연중생산이 가능하다. 그러므로 우리는 동충하초 재배농가가 누에 품종 중 금옥잠을 이용하고 연중생산 기술을 활용하여 소득증대를 이룰 수 있을 것으로 기대한다. There is no specific data on Entomogenous fungus (DongChungHaCho) in accordance with the silkworm varieties, despite of very high value industrial use as functional materials at domestic and abroad. In this study, we investigated culture condition and characteristics of Paecilomyces tenuipes in 3 silkworm cultivars and 2 specific silkworm breeds. Infection rate of P. tenuipes for silkworm varieties was the highest Kumokjam, followed by Baegokjam, Daeseongjam, Golden silk, Yeonnokjam in that order. The optimum culture conditions were as follows: dark condition, 22oC ± 1, and about 4 cm2 in planting density per pupa. Also, we have developed annual production technologies of P. tenuipes. First, in order that the moisture content of the infection pupa became 3% or less in the natural condition. Second, the dried pupa must be vacuum-packed and was kept under 4oC. Finally, by restoring the moisture content of the dried pupa and culture in optimal condition, the technique for annual production of the P. tenuipes was achieved. Therefore, we expect that the use of Kumokjam and annual production technique will contribute significantly to farm income.

혼합 실크 피브로인막의 투명도

조유영 ( You Young Jo ),권해용 ( Hae Yong Kweon ),여주홍 ( Joo Hong Yeo ),이광길 ( Kwang Gill Lee ) 한국잠사학회 2013 한국잠사곤충학회지 Vol.51 No.2

Silk fibroin is a natural biomaterial that has the biocompatibility and other many advantages. But as a silk fibroin membrane thickness increases, the transparency becomes more opaque. Because the transparency of membranes tissue such as the cornea and dura mater are necessary, transparent membrane is required to replace these transparent membranes. In this study, we fabricated blending silk fibroin membranes that made by mixing the various inorganic salts or polymer in an aqueous solution of silk fibroin. The transparency of the membranes were analyzed. the transparency of these membranes is very different, depending on the mixed materials. Inorganic salts mixed silk membrane was more transparent than the polymer mixed one. Especially, the silk fibroin membrane with calcium chloride was very transparent. We showed the possibility of blending silk fibroin membrane, which can be used in perfect transparent membrane such as the cornea. In the future, we expect that the transparent blending silk fibroin membrane can be used in various medical applications.

실크피브로인 용해조건에 따른 생체막의 물성 변화

조유영 ( You Young Jo ),권해용 ( Hae Yong Kweon ),이광길 ( Kwang Gill Lee ),이희삼 ( Heui Sam Lee ) 한국잠사학회 2012 한국잠사곤충학회지 Vol.50 No.2

Silk is a natural polymer that has the advantages of the biocompatibility, excellent mechanical strength, low immune rejection, and molding facility. But silk does not dissolve easily in water or general solvent. To investigate the characteristics of silk biological membranes according to dissolving condition of silk fibroin, we made the silk biological membranes using silk fibroin solutions with different amount and dissolving time of silk. The characterizations of the silk biological membranes such as morphology, structure, and mechanical strength were observed. Although each biological membrane has the same fibroin content, there was a significant difference in the thickness and transparency. But there was no significant change in the molecular weight of the silk fibroin solutions and morphology of silk biological membranes. We were established the manufacturing condition for silk fibroin biological membrane. So we expect that the conditions will help in the development of medical supplies in the future.

LED광을 이용한 눈꽃동충하초 생산

조유영 ( You Young Jo ),권해용 ( Haeyong Kweon ),이광길 ( Kwang Gill Lee ),이희삼 ( Heui Sam Lee ),여주홍 ( Joo Hong Yeo ) 한국잠사학회 2014 한국잠사곤충학회지 Vol.52 No.1

최근 다양한 파장의 LED를 이용한 작물의 생장과 기능성 물질의 변화에 대한 연구가 활발히 진행되고 있다. 본 연구에서는 LED 조사가 눈꽃동충하초 생산에 미치는 영향을 알아보고자, 청색LED, 적색LED, 형광등 아래에서 눈꽃동충하초를 재배하여, 자실체 형성율, 자실체 개수, 자실체 길이 등을 관찰하였다. 그 결과, 적색 LED 광에서 재배한 눈꽃동충하초의 자실체 발생율은 100%에 달하였으며, 뿐만 아니라 형성된 자실체의 개수가 37%까지 증가하는 등 자실체 무게, 자실체 길이 등 모든 항목에서 우수한 결과를 보여주었다. 하지만 청색 LED광에서는 오히려 생육을 저해하는 결과를 나타내었다. 따라서, 눈꽃동충하초 재배 시, 적색 LED를 조사한다면 농가의 소득 향상에 도움을 줄 것으로 여겨진다. The effects of LED irradiation on growth and functional components of crops are actively being studied. We investigated the effects of red LED on the cordyceps cultivation because Cordyceps is very difficult to find in the wild and to cultivation. The synnemata formation rate of P. tenuipes was 100% under red LED. Also weight, length, and number of the synnemata were significantly increased. We expect that the red LED treatment will be of help to improve farm income.

Transcoder에서 다양한 해상도 변환을 위한 개선된 cubic convolution scaler

유영조(You Young-Jo),서주헌(Seo Ju-Heon),한종기(Han Jong-Ki) 한국통신학회 2007 한국통신학회 학술대회논문집 Vol.2007 No.7

H.264에서 개선된 조기 종료알고리즘을 이용한 효율적인 움직임 예측

유영조(You Young-Jo),김성은(Kim Sung-Eun),김상미(Kim Sang-Mi),한종기(Han Jong-Ki) 한국통신학회 2007 한국통신학회 학술대회논문집 Vol.2007 No.7

인체적용을 위한 실크 스펀지의 제조 및 특성

조유영 ( You Young Jo ),권해용 ( Hae Yong Kweon ),이광길 ( Kwang Gill Lee ),여주홍 ( Joo Hong Yeo ),이희삼 ( Heui Sam Lee ) 한국잠사학회 2013 한국잠사곤충학회지 Vol.51 No.1

Fibroin and sericin are natural proteins obtained from cocoon and one of the spotlight materials for medical device. Medical device made of these proteins also has the advantage that this material is biodegradable in to amino acid. In this study, we prepared silk sponges using fibroin, sericin and additives. The characterizations of the silk sponges such as morphology, stability, and blood absorbency were observed. The structural stability of the silk sponge decreased significantly by increasing sericin contents. The effect on the concentrations of ethanol to induce crystallization was observed to be superior to 70% ethanol. Structural stability of silk sponges containing additives was very lower than those not containing additives. The blood absorbency of the silk sponges was found to be excellent, regardless of the composition of sericin and fibroin. The resilient power of these sponges was also very good, in spite of the repeated soaking and drying. Therefore, we expect that the silk sponges can be used medical supplies such as plastic implants and hemostatic cotton.

천연물 / 유기화학 : 실크 생체막에 대한 세포 부착 및 세포 증식

조유영 ( You Young Jo ),권해용 ( Hae Yong Kweon ),이광길 ( Kwang Gill Lee ),남성희 ( Sung Hee Nam ),이희삼 ( Heui Sam Lee ),여주홍 ( Joo Hong Yeo ) 한국응용생명화학회 2011 Journal of Applied Biological Chemistry (J. Appl. Vol.54 No.3

Silk fibroin, a natural protein produced by silkworm, is a good biomaterial which has biodegradability and biocompatibility. To ascertain the effects of silk fibroin on cell growth, silk fibroin films were prepared using silk fibroin aqueous solutions of various concentrations. We investigated the attachment, proliferation, morphology of the cells and the expression levels of genes related to cell attachment and growth on the silk fibroin films. When the cells were cultured on the 0.1 and 1% silk fibroin film, the cell adhesion ability was very excellent. Particularly, overall cell growth on the 1% silk fibroin film was definitely superior to the others. Also, expression levels of genes related cell growth were increased on the 0.1 and 1% silk fibroin film. These results suggest silk as a material for medical applications.

연골 재생에 대한 실크 단백질의 가능성

조유영 ( You Young Jo ),권해용 ( Hae Yong Kweon ),이광길 ( Kwang Gill Lee ),이희삼 ( Heui Sam Lee ),전정우 ( Jeong Woo Chon ) 한국잠사학회 2012 한국잠사곤충학회지 Vol.50 No.1

A number of researcher have studied biomaterials for cartilage regeneration and are now proceeding. Silk protein was attempted for use as biomedical materials by many researchers because it is natural polymer with biocompatibility and excellent mechanical strength. In this study, we want to know a possibility of silk protein on the cartilage regeneration. We isolated chondrocytes from nasal cartilage and confirmed optimal culture condition of the cells. To observe the effects of silk fibroin on chondrogenesis, we added silk fibroin solutions to the culture medium of chondrocyte and detected gene expression levels related chondrogenesis such as col2, col10. The chondrocytes showed optimal growth when they were cultured in DMEM medium supplemented with 10% FBS 100 ·?M ascorbic acid. The levels of col2 gene expression were increased in non-autoclaved silk fibroin, but decreased in autoclaved one. Also the gene expression levels of col10 were increased in silk fibroin, particulary at 3D culture. Based on the results of this study, we had seen the possibility of silk fibroin for cartilage regeneration. In future studies, we should know more clearly the relationship between cartilage regeneration and the silk protein.

동결건조 누에분말의 일반 성분 및 영양 성분

권해용,조유영,김현복,주완택,이지혜,Kweon, HaeYong,Jo, You-Young,Kim, Hyunbok,Ju, Wan-Tack,Lee, Ji Hae 한국잠사학회 2019 한국잠사곤충학회지 Vol.55 No.2

혈당 조절에 도움을 줄 수 있는 건강기능식품 기능성 원료로 인증을 받은 동결건조 누에분말의 일반 성분 및 영양 성분에 대하여 보고된 바를 고찰하여 다음과 같은 결론을 얻을 수 있었다. 1. 동결건조 누에분말의 일반 성분은 조단백질 57.7%, 조지방 9.5%, 조섬유 5.4%, 회분 9.3%, 수분 4.7% 내외였다. 식육성 재료나 식용 곤충에 비하여 단백질 성분이 높고 지방 성분은 낮았다. 2. 동결건조 누에분말을 이루고 있는 주요 아미노산은 glutamic acid와 aspartic acid로 아미노산의 조성은 누에의 성장 단계에 따른 차이가 있었다. 3. 동결건조 누에분말은 포화지방산과 불포화지방산이 약 3:7의 비율로 있으며, linolenic acid 34.7%, oleic acid 24.4%, palmitic acid 20.2%, stearic acid 9.2%, linoleic acid 7.7%가 많이 포함되어 있다. 4. 동결건조 누에분말에 많이 포함되어 있는 5대 무기물은 칼륨, 인, 칼슘, 마그네숨, 황 등이 있으며, 그 외에도 구리, 망간, 아연, 철 등의 무기물을 가지고 있다. Freeze-dried silkworm powder has been approved as a functional ingredients for blood glucose control since 2009. Proximate and nutrient compositions of silkworm powder was summerized as bellow. Silkworm powder contained 4.7% moisture, 57.7% crude protein, 9.5% crude fat, 5.4% crude fiber, and 9.3% ash. The larval protein contained 18 known amino acid including glutamic acid and aspartic acid. Fat is composed of 3:7 ratio of saturated fatty acid to unsaturated fatty acid including various essential fatty acids. It contained 34.7 linolenic acid, 24.4% oleic acid, 20.2% palmitic acid, 9.2% stearic acid, and 7.7% linoleic acid. Five minerals, K, P, Ca, Mg, S were abundant in the silkworm powder with other minerals such as Cu, Mn, Fe, and Zn.