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RISS 인기검색어
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- 저자 : 오창호 ( Sang Sun Lee (검색결과 3 건)
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오창호,이태수,이상선,백기엽 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1998 연구보고서 Vol.3 No.-
한국의 춘란 자생지의 춘란(Cymbidium goeringii) 뿌리에서 공생하는 10개의 균근균을 분리하였다. 분리된 난 균근균을 PDA에서 배양한 결과 균총은 다양한 색깔을 나타내었으며, 생장 속도도 달랐다. 분리된 난 균근균을 현미경으로 관찰한 결과 Rhizoctonia repens 또는 R. endophytica var endophytica로 동정되었다. R. repens는 자생란인 춘란의 뿌리에서 많이 분리되었으며, R. endophytica var endophytica는 상업적으로 재배되는 외국산의 난 뿌리에서 분리되었다. 분리된 균들 중에 R. repens와 유사한 5개의 분리균을 oatmeal agar에 접종하고 배양한 후 여러종의 난 유묘를 이식하여 난의 생장을 관찰하였다. 그 결과 난의 생장은 분리 균에 따라서 다양하였으나 대부분의 난은 생장촉진 반응을 나타내서, 분리균과 각각의 난과의 공생 관계를 관찰할 수 있었다. Ten isolates of the orchid mycorrhizal fungi were isolated from the roots of Korean native orchid plants (cymbidium goeringii) which inhabitate mainly in southern and western areas of Korea. The growth rates and color of the isolates in potato dextrose agar (PDA) were various. Microscopic observations of the hyphae isolated were identified as Rhizoctonia repens and R. endophytica var endophytica or their related species. R. repens was isolated from the roots of the Korean native orchids, but R. endophytica var endophytica was only isolated from the roots of the commercial orchids introduced from foreign countries. Also, the polymorphic patterns of genomic DNA extracted from selected isolates were compared with those of DNA extracted from the orchid mycorrhizal fungi isolated previously and similar band patterns were observed among those isolates. Five isolates of R. repens were selected and cultured at the oatmeal agar for investigating their symbiosis with orchid plants. The symbiotic specificity between orchid plants and isolated orchid mycorrhizal fungi was observed by growing orchids about six months in the greenhouse. The symbiotic responses of the commercial orchid plants with selected isolates were quite different from different isolates due to the genetic variations.
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백기엽,오창호,선정훈,이상선 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1997 연구보고서 Vol.2 No.-
For cultivations of orchid plants, the Korean native orchid plant inhabiting (KNO) soils were collected from the Southern and Western areas in Korea, and employed for understanding the role of symbiotic fungi on the orchids. The parameters related to growth of the orchid plant were investigated after the young nurseries (plantlets) of Cymbidium goeringii, C. kanran, and Neofinetia falcata planted on the sterilized or unsterilized soils of KNO under the conditions of green house. The fresh weights or dried weights of all orchid plants grown on the unsterilized soil for 17 months were highly heavier than those grown on the sterilized soils. Thus, the differences of the plant growth were not found among the four different KNO soils. The various stages of peletons in the root cells of the orchid plants were distinguished from unsterilized soils, while those not quite often in the sterilized soils. Mineral nutrients (K, Ca, Mg, P) related to nitrogen were also measured in the individual plantlets for understanding the plant symbiosis. The nitrogen of the orchid plants absorbed in the unsterilized soils were measured to be comparatively greater than those in sterilized soils during the orchid plant growth. The other minerals of the orchid plants absorbed in the unsterilized soils were also measured to be comparatively greater than those in the sterilized soils. The metabolism of nitrogen were concluded to be much important, and were considered to stimulate the other mineral absorptions in growth of the orchid plants symbiotic with the fungi.
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