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계대 및 배양액이 슈반세포의 증식과 표현형에 미치는 영향
오아영 ( A Young Oh ),김순희 ( Soon Hee Kim ),정수현 ( Su Hyun Jung ),홍현혜 ( Hyun Hye Hong ),전나리 ( John M. Rhee ),이종문,신형식 ( Hyung Sik Shin ),강길선 ( Gil Son Khang ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.3
Schwann cell(SCs) has been widely accepted to play indispensable roles during neural development and regeneration but obtaining and culture of SCs is difficulty. SCs have been characterized depending on sequential passages and medium. We measured proliferation and phenotypical stability of SCs using three medium(growth medium, purification medium, high density culture medium). Morphological changes were observed by an optical microscope and cell proliferation was counted using hemacytometer. The effect of sequential passage and medium on the phenotypical stability of SCs was assessed RT-PCR and staining for NF and S-100 as SCs markers. In result, proliferation of SCs showed no difference between three groups up to passage 3. NF and S-100 markers were high expressed in up to passage 3 in PM and HDCM. We concluded that SCs of passage 1, 2 and 3 cultured in HDCM will be useful to cell therapy using SCs or spinal cord regeneration using SCs hybrid scaffold.
케라틴을 함유한 PLGA 지지체가 슈반세포의 증식 및 특성 유지에 미치는 영향
오아영 ( A Young Oh ),김순희 ( Soon Hee Kim ),정수현 ( Su Hyun Jung ),홍현혜 ( Hyun Hye Hong ),전나리 ( Na Ri Jeon ),( Sang Jin Lee ),( Mark Van Dyke ),( James J. Yoo ),이종문 ( John M. Rhee ),강길선 ( Gilson Khang ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4
Keratin is the major structural fibrous protein such as wool, hair, and nail and is useful as natural protein. Schwann cells(SCs) can stimulate tissue sparing and enhance outgrowth of both intact and lesion axons. We were prepared keratin/PLGA(0, 20 and 50 wt%) scaffolds by solvent casting/salt leaching method. Cellular viability and proliferation were assayed by MTT test. Morphology of cellular adhesion were confirmed by scanning electron microscope(SEM). SCs specific protein was assessed staining by s-100 for SCs marker and RT-PCR was conducted to confirm mRNA expression of NF, P-75 and s-100 for SCs marker. In MTT assay results, cell viability in scaffolds impregnated 20 wt% of keratin were higher than other scaffolds. SEM exhibited normal spindle-shaped morphology on 20 wt% of keratin. SC specific mRNA expression and protein could not be observed in scaffold containing 50 wt% of keratin. These results suggest that keratin provide suitable surface to neural cells and proper content affect on culture condition to improve cell adhesion and proliferation.
3D 형태의 PLGA 지지체에서 뷰틸레이트하이드록시애니솔의 방출거동 및 골수간엽줄기세포의 신경세포화
오아영 ( A Young Oh ),김순희 ( Soon Hee Kim ),모종현 ( Jong Hyun Moh ),김근아 ( Geun Ah Kim ),이종문 ( John M. Rhee ),이해방 ( Hai Bang Lee ),강길선 ( Gil Son Khang ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.2
Bone marrow stromal cells(BMSCs) can be differentiated into chondrocytes, osteocytes, neuronal cells under the controlling of culture condition. We fabricated butylated hydorxyanisole(BHA) loaded poly(L-lactide-coglycolide) (PLGA) scaffolds(BHA/PLGA) for neurogenesis of BMSCs and to observe the release behavior of BHA. BHA/PLGA scaffold were manufactured as the concentrations of 200, 400, and 600 μM of BHA by solvent casting/ salt leaching method. We isolated and cultured BMSCs from the tibias of adult female Fischer rat, we seeded BMSCs into the inner core of the hybrid scaffold. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazol- 2yl)-2,5-diphenyl-2H- tetrazolium bromide(MTT) test. Morphology of celluar adhesion were confirmed by scanning electron microscope SEM). The effect of BHA/PLGA scaffold on the neural differentiation of rat BMSCs were assessed RT-PCR. According to results, aspect of release of BHA in BHA/PLGA matrix showed that the initial burst of BHA was lower in scaffold than film and released continuously during the desired period in delivery system. BHA 600 μM loaded scaffold revealed low cell viability by MTT assay results. Mophology of BMSCs changed like a neural cell in 200 and 400 μM BHA/PLGA by SEM observation. In addition, 200 and 400 μM BHA/ PLGA scaffold showed higher expression of neural markers. We conclude that neural differentiation of BMSCs in BHA/PLGA scaffold could be possible for long period.
케라틴/PLGA 지지체가 골수간엽줄기세포의 신경분화에 미치는 영향
오아영 ( A Young Oh ),김순희 ( Soon Hee Kim ),김초민 ( Cho Min Kim ),정수현 ( Su Hyun Jung ),전나리 ( Na Ri Jeon ),홍현혜 ( Hyun Hye Hong ),( Sang Jin Lee ),( Mark Van Dyke ),( James J Yoo ),이동원 ( Dong Won Lee ),이종문 ( 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.1
Bone marrow stromal cells(BMSCs) exhibit multiple traits of a stem cell population, and they can expand many times in vitro and be induced to differentiate into multiple cell types. Keratin is the major structural fibrous protein providing outer covering such as wool, hair, and nail. We think keratins are useful as natural biomaterial. We examined the effect of Keratin/PLGA scaffold on the neural differentiation of rat BMSCs. We developed the keratin loaded poly(L-lactide-co-glycolide)(PLGA) scaffolds. Keratin/PLGA 0, 20 and 50 wt% scaffolds were prepared by solvent casting/salt leaching method. BMSCs were harvested from the femurs of adult female Fischer rat. These cells were seeded in prepared Keratin/PLGA scaffold and cultured in Medium 50 ml DMEM, 2 %DMSO, 200 ?M BHA, 25 ?M valproic acid, 10 ?M forskolin, 1 ?M hydro-cotisone, 5?g/ml insulin for 5 day. The effect of Keratin/PLGA scaffold on the neural differentiation of rat BMSCs were assessed in culture using the MTT assay and RT-PCR was conducted to confirm mRNA expression of NSE and Nf for neural marker. We confirmed that effect of Keratin/PLGA scaffold on the neural differentiation of rat BMSCs. According to our results, 20 wt% keratin/ PLGA scaffold have good cell compatibility and the expression of neural markers was higher. In conclusion, Keratin/ PLGA scaffold, on which neural differentiation of rat BMSCs was possible.
증례보고 : 소아에서 전신 마취 후 발생한 일과성 악하선 종창
박희연 ( Hee Yeon Park ),오아영 ( Ah Young Oh ),윤혜정 ( Hye Jeong Yun ) 대한마취과학회 2007 Korean Journal of Anesthesiology Vol.53 No.4
Acute transient salivary gland swelling is a rare complication of general anesthesia. These swellings usually resolve over a few hours without sequelae. The etiology of the salivary gland swelling is unclear. However, they are considered to be associated with cough or straining on the endotracheal tube and mechanical blockage of salivary gland duct. Anesthesiologists should know the possible causes, lack of clinical significance, and the course of these transient salivary gland swellings in order to reassure the patient. We report bilateral submandibular gland swelling developed after endotracheal anesthesia in a 4-year-old girl. (Korean J Anesthesiol 2007; 53: 532∼4)
증례보고 : 상부기관협착증을 가진 환자의 기관절제와 단단문합술에서의 후두마스크를 사용한 마취관리
권태명 ( Tae Myoung Kwon ),윤미자 ( Mi Ja Yun ),강종만 ( Jong Man Kang ),오아영 ( Ah Young Oh ) 대한마취과학회 2006 Korean Journal of Anesthesiology Vol.51 No.4
Tracheotomy and end-to-end anastomosis is a relatively rare operation, but it is one of the most challenging for anesthesiologists. During surgery, the principal anesthetic consideration is to maintain the ventilation and oxygenation throughout the procedure. We experienced anesthetic management of a 49-year-old man with upper tracheal stenosis after long-term intubation and ventilation care. The stenotic lesion was located at 2 cm below the vocal cords, and the length of the stenotic segment was about 2 cm. The anesthesia was induced and maintained with intravenous propofol and alfentanil. The airway was managed with LMA and distal tracheal intubation on the surgical field and we were able to maintain adequate ventilation and oxygenation throughout the operational period. (Korean J Anesthesiol 2006; 51: 499~503)