국내산 시판 멸치(Engraulis japonicas) 액젓의 품질평가

엄인선,서정길,김희대,박권삼 한국수산과학회 2018 한국수산과학회지 Vol.51 No.6

We investigated the quality of 12 commercial salted and fermented anchovy Engraulis japonicas sauces by measuring their chemical compositions, bacteria concentrations, and biogenic amine contents. The sauces had a moisture content of 67.13-69.83% (mean: 68.17%), salinity of 20.00-25.84% (mean: 22.29%), pH of 5.14-6.28 (mean: 5.63), volatile basic nitrogen content of 119.12-273.37 mg/100 g (mean: 199.19 mg/100 g), total nitrogen content of 0.82- 1.50% (mean: 1.30%) and amino nitrogen content of 550.17-1,086.62 mg/100 g (mean: 774.99 mg/100 g). The viable cell counts ranged from not detected to 1.6×103 CFU/mL, and the number of biogenic amine-forming bacteria was very low or undetectable. The tested samples contained 372.32-2,111,61 mg/kg (mean 813.48 mg/kg) histamine, 29.62-144.29 mg/kg (mean 98.14 mg/kg) cadaverine, 87.89-530.84 mg/kg (mean 329.91 mg/kg) tryptamine, 20.89- 127.17 mg/kg (mean 60.49 mg/kg) putrescine, and 13.08-109.91 mg/kg (mean 57.74 mg/kg) tyramine. Whereas no spermidine or spermine was detected in any sample. These results strongly suggest the necessary of monitoring the biogenic amine contents of commercial salted and fermented anchovy sauces carefully to ensure consumer health.

곰소만 해역 어패류의 중금속 오염도 조사

엄인선,이동수,신일식,박권삼 한국수산과학회 양식분과 2017 한국수산과학회 양식분과 학술대회 Vol.2017 No.5

시판 까나리(Ammodytes personatus) 액젓에서 putrescine 생성균의 분리 및 특성

엄인선 ( In-seon Um ),김태옥 ( Tae-ok Kim ),박권삼 ( Kwon-sam Park ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.5

Bacterial decarboxylation of amino acids in food leads to the production of biogenic amines, which can cause reactions in human that include headaches, nausea, palpitations, chills, and severe respiratory distress. The amine putrescine is an especially effective inhibitor of metabolizing enzymes and amplifies histamine intoxication and tyramine poisoning. Using an L-ornithine decarboxylating medium, we isolated 14 putrescine-producing bacteria from sand lance, Ammodytes personatus, sauces. The isolates were identified, using an API kit and 16S rRNA analysis, as Lysinibacillus fusiformis (1 strain), Lysinibacillus xylanilyticus (6 strains), Lysinibacillus macroides (1 strain), Lysinibacillus sphaericus (3 strains), Bacillus fusiformis (1 strain), Paenibacillus favisporus (1 strain), and Staphylococcus caprae (1 strain). These strains produced between 1.66 to 236.97 μg/mL of putrescine after 48 h incubation. Lysinibacillus spp. were the dominant putrescine-producing bacteria in sand lance sauces, which produced 236.97 μg/mL of putrescine from a culture broth containing 0.5% L-ornithine. This is the first report on the isolation and identification of putrescine-producing bacteria from sand lance sauces.

시판 까나리(Ammodytes personatus) 액젓에서 분리한 tryptamine 생성균의 특성

엄인선 ( In-seon Um ),김태옥 ( Tae-ok Kim ),김희대 ( Hee-dai Kim ),박권삼 ( Kwon-sam Park ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.6

We isolated seven tryptamine-producing bacteria from commercial salted and fermented sand lance (Ammodytes personatus) sauces using an L-tryptophan decarboxylating medium. These tryptamine-producing bacteria, identified using an API kit and 16S rRNA analysis, included Lysinibacillus xylanilyticus (one strain), Lysinibacillus fusiformis (four strains), and Staphylococcus epidermidis (two strains). Lysinibacillus spp. produced the highest levels of tryptamine in culture broth containing 0.5% L-tryptophan, compared with 1.0% and 2.0% preparations. After 72 h of incubation, Staphylococcus epidermidis produced the highest levels of tryptamine (60.50 μg/mL and 664.86 μg/ mL) in culture broth containing 2.0% L-tryptophan. While Lysinibacillus spp. comprised the dominant tryptamine-producing bacteria in sand lance sauces, Staphylococcus epidermidis also showed high tryptamine-producing active-ity. This is the first report on the isolation and identification of tryptamine-producing bacteria in sand lance sauces.

시판 까나리(Ammodytes personatus) 액젓의 biogenic amines 함량

엄인선 ( In Seon Um ),박권삼 ( Kwon Sam Park ) 한국수산과학회 2015 한국수산과학회지 Vol.48 No.6

This study investigated the quality of seven commercial salted and fermented sand lance Ammodytes personatus sauces by measuring their chemical compositions, bacteria concentrations, and biogenic amine contents. The sauces had a 63.97-67.32% (mean: 65.72%) moisture content, 23.16-24.03% (mean: 23.60%) salinity, 5.51-6.14 (mean: 5.81) pH, 164.76-217.98 mg/100 g (mean: 182.97 mg/100 g) volatile basic nitrogen, 0.94-1.42% (mean: 1.24%) total nitrogen, and 673.77-791.86 mg/100 g (mean: 720.76 mg/100 g) amino nitrogen content. Viable cell counts ranged from 1.2×101 to 2.9×102 CFU/mL, and number of biogenic amine-forming bacteria was very low or not detected. Overall, the tested samples had average levels of histamine of 301.02 mg/kg, cadaverine of 29.78 mg/kg, tyramine of 199.21 mg/kg, putrescine of 183.00 mg/kg, and tyramine of 13.01 mg/kg. This strongly suggests that it is necessary to monitor the biogenic amine contents of commercial salted and fermented sand lance sauces carefully to ensure consumer health.

곰소만 해역 어패류의 미생물학적 위해요소 조사

이동수,엄인선,박권삼,신일식 한국수산과학회 양식분과 2017 한국수산과학회 양식분과 학술대회 Vol.2017 No.5

곰소만 해역의 바지락(Ruditapes philippinarum)에서 분리한 대장균 (Escherichia coli)의 항균제 내성 및 병원성 유전자의 보유성

김태옥 ( Tae-ok Kim ),엄인선 ( In-seon Eom ),박광호 ( Kwang-ho Park ),박권삼 ( Kwon-sam Park ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.6

In total, 151 Escherichia coli isolates from Ruditapes philippinarum in Gomso Bay were analyzed for their susceptibility to 18 different antimicrobial agents and for genes associated with virulence. For virulence genes, each strain of the isolates was positive for the enterotoxigenic E. coli (ETEC)-specific heat-stable toxin (estA), enteroinvasive E. coli (EIEC)-specific invasion-associated locus (iaa) gene and enteropathogenic E. coli (EPEC)-specific attaching and effacing (eae) gene. According to a disk diffusion susceptibility test, resistance to ampicillin was most prevalent (23.2%), followed by resistance to amoxicillin (22.5%), ticarcillin (20.5%), tetracycline (18.5%), nalidixic acid (12.6%), ciprofloxacin (10.6%), streptomycin (9.9%), and chloramphenicol (6.6%). More than 35.8% of the isolates were resistant to at least one antimicrobial agent, and 19.9% were resistant to four or more classes of antimicrobials; these were consequently defined as multidrug resistant. Minimum inhibitory concentration (MIC) ranges for the antimicrobial resistance of the 15 different antimicrobial agents of 54 E. coli strains were confirmed by varying the concentrations from 32-2,048 μg/mL. Overall, these results not only provide novel insights into the necessity for seawater and R. philippinarum sanitation in Gomso Bay but they also help to reduce the risk of contamination by antimicrobial-resistant bacteria.

곰소만 해역 해수에서 분리한 장염비브리오(Vibrio parahaemolyticus)의 항균제 내성 및 최소발육억제농도의 규명

김태옥 ( Tae-ok Kim ),엄인선 ( In-seon Um ),김희대 ( Hee-dai Kim ),박권삼 ( Kwon-sam Park ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.5

Seventy-nine Vibrio parahaemolyticus isolates from surface seawater from Gomso Bay, west coast of Korea, were analyzed for the presence of virulence genes and their susceptibility to 30 different antimicrobials. All 79 isolates were examined for the presence of two virulence genes (tdh or trh) using polymerase chain reaction (PCR); however, no isolates possessed either the tdh or trh gene. According to a disk diffusion susceptibility test, all of the strains studied were resistant to oxacillin, penicillin, and vancomycin, followed by ticarcillin (97.5%), ampicillin (96.2%), clindamycin (86.1%), erythromycin (10.1%), streptomycin (7.6%), cefoxitin (6.3%), amikacin (2.5%), and cephalothin (2.5%). However, all of the strains were susceptible to 19 other antimicrobials including cefepime, cefotaxime, chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazole/trimethoprim, and trimethoprim. All 79 isolates (100%) were resistant to four or more classes of antimicrobials, and two strains exhibited resistance to eight antimicrobial agents. The average minimum inhibitory concentrations (MICs) for V. parahaemolyticus for ampicillin, penicillin, ticarcillin, and vacomycin were 946.5, 1,305.9, 1,032.3, and 45.0 μg/mL, respectively.

완도해역 해수에서 분리한 장염비브리오(Vibrio paragaemolyticus)의 항균제 내성 및 병원성 유전자의 특징

김태옥 ( Tae Ok Kim ),엄인선 ( In Seon Ewn ),조상만 ( Sang Man Jo ),김희대 ( Hee Dai Kim ),박권삼 ( Kwon Sam Park ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.3

Sixty-seven Vibrio parahaemolyticus isolates from surface seawater from the Wando area, on the southern coast of Korea, were analyzed for their susceptibility to 15 different antimicrobials and the presence of virulence genes. According to the disk diffusion susceptibility test, all of the strains studied were resistant to ampicillin and oxacil-­lin, while decreasing percentages were resistant to vancomycin (64.2%), streptomycin (56.7%), amikacin (31.3%), kanamycin (22.3%), cephalothin (20.9%), erythromycin (10.4%), ciprofloxacin (4.5%), and tetracycline (3.0%). All of the strains were susceptible to five antimicrobials: chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazolel/ trimethoprim, and trimethoprim. Fifty-nine isolates (88.1%) were resistant to three or more classes of antimicrobial and defined as multidrug resistant, and two strains were resistant to seven antimicrobial agents. The minimum inhibi-tory concentration (MIC) of the 67 V. parahaemolyticus isolates to ampicillin and oxacillin ranged from 512-2,048 and 64-512 ㎍/mL, respectively. All 67 isolates were also examined for the presence of the tdh and trh virulence genes using the polymerase chain reaction (PCR). However, no isolates possessed either tdh or trh. The VPA0477 (β-Iactamase) gene, present in all of the tested strains, was validated as a new specific marker gene in PCR assays for the accurate detection and identification of V. parahaemolyticus.

외상성 두부 손상 아동의 인지기능 특성 및 어머니의 양육스트레스에 관한 예비연구

오미령 ( Mi-ryung Oh ),심규원 ( Kyu-won Shim ),김동석 ( Dong-seok Kim ),박은경 ( Eun-kyung Park ),염인선 ( In-sun Yeom ),신의진 ( Yee-jin Shin ),엄소용 ( So-yong Eom ) 한국영유아아동정신건강학회 2012 영유아아동정신건강연구 Vol.5 No.1

본 연구의 목적은 외상성 두부 손상 아동의 인지 기능과 양육스트레스에 대한 임상적 특성을 파악 하는 것이었다. 이를 위해 한국판 웩슬러 아동용 지능검사, Rey-Kim 아동용 기억검사, 전산화된 주의력장애 진단 시스템(시각), 한국판 부모양육 스트레스검사를 사용하였다. 서울소재 대학병원에 외 상성 두부 손상을 주호소로 내원한 만 6세에서 15세에 해당하는 아동 중 심리평가에 의뢰된 아동 66명과 보호자 66명을 대상으로 자료를 수집하여 분석한 결과, 두부 손상으로 내원한 아동의 지능 평균은 평균 수준에 해당하였고, 기억 능력 평균은 평균하 상단 수준에 해당하고 있어 지능수준에 비해 통계학적으로 유의미한 수준의 저하가 시사되었다. 주의력의 경우 부주의, 충동성, 반응시간 표준편차의 영역의 평균 자체가 비정상 범위에 속하고 있으며, 과반수이상이 비정상 범위에 포함 되는 것으로 나타났다. 의식 소실의 유무로 집단을 나누어 비교해본 결과 의식 소실 경험의 아동은 전체 지능 및 동작성 지능에서 유의미한 저하를 보였으나 기타 영역에서는 두 집단의 차이가 나타나지 않았다. 어머니의 양육 스트레스 검사 결과 과반수이상이 정상 범위를 벗어나는 스트레스에 해당하고 있어 임상적으로 도움이 필요한 스트레스를 경험하고 있는 것으로 나타났다. 하위 영역 중 양육 유능감, 아동에 대한 기대, 자녀와의 애착, 역할의 제한의 영역에서 상대적으로 증가된 스트레스를 보이고 있었다. 의식 소실의 여부에 따라서는 통계적으로 의미 있는 차이를 보이지는 않았다. 이러한 두부 손상 아동들의 특성을 고려하여, 의학적 서비스와 더불어 학습 치료적 개입, 부모교육 프로그램 등과 같은 심리학적 서비스의 통합적 개입 계획 수립이 필요함이 시사되었다. The purpose of this study is to investigate the clinical characteristics of cognitive function and parenting stress found in children with traumatic brain injuries. This study was conducted using Korean-Wechsler Intelligence Scale for Children, Rey-Kim Memory Test for children, attention deficit hyperactivity disorder diagnostic system, and Korean Parenting Stress Index. Subjects were 66 children with traumatic brain injuries at the age of six to fifteen who had visited hospitals in Seoul and their guardians. The result showed that intelligence of the children with traumatic brainin juries was at the average level and their memory function was lower than the average level, indicating that memory function might relatively decline. In the case of visual attention, the standard deviation of inattention, impulsivity, and reaction time was higher than the T score. The result also showed that more than half of the subjects were placed in the higher threshold. The subjects were divided into two groups based on whether they had ever experienced loss of consciousness. Comparison of the two groups found that the children who had experienced loss of consciousness showed significantly lower levels of Full Scale Intelligence Quotient and Performance Intelligence Quotient. However, no significant difference was found in the remaining domains. More than half of the subjects’ mothers experienced parenting stress that was higher than the normal level. Considering the characteristic of the children with traumatic brain injury, it is required that an integrated intervention plan for psychological services such as supportive therapy for family and programs for parent education should be provided in addition to medical services.