종자검정 발전의 역사

양미희,소은희 한국종자연구회 2010 종자과학과 산업 Vol.7 No.2

한국산 배(梨)의 항돌연변이 및 항암효과

양미희,박장환,김대중,정헌상 대한암예방학회 2005 Journal of cancer prevention Vol.10 No.2

We already reported that consumption of Korean pears had inverse association with level of urinary 1-hydroxypyrene (1-OHP), which is an exposure biomarker and metabolite of polycyclic aromatic hydrocarbons (PAHs). As PAHs include various carcinogens such as benzo(a)pyrene, our report showed the chemopreventive potential of Korean pears. Recently, we studied effects of Korean-pears on PAH-induced mutagenicity and carcinogenicity, i.e. antimutagenic effects with MN (micronuclei) test and Comet assay, and anticarcinogenic effects with animals. Finally, we performed an intervention study with the pears in volunteers using toxicokinetic methods. As results, we found that Korean pears have antimutagenic, antioxidative, and anticarcinogenic effects against PAHs exposure and rapid excretion of parental PAHs. Therefore, the intake of pears after PAHs exposure such as BBQ is thought as an ideal lifestyle to prevent PAH-induced cancers. (Cancer Prev Res 10, 124-127, 2005)

Development and application of biomarkers for monitoring of environmental toxicants in humans

양미희 환경독성보건학회 2006 한국독성학회 심포지움 및 학술발표회 Vol.- No.-

Individual Variations in Toxicity of Tobacco Smoking

양미희 대한암예방학회 2005 Journal of cancer prevention Vol.10 No.3

Tobacco smoking is a well-known cause of cancers and has been emphasized not only to clarify the mechanism of carcinogenesis but also to prevent the smoking-related cancers. In addition, the Korean female passive smokers showed early onset of lung cancer and high incidence of adenocarcinoma compared to males. Therefore, several factors are suspected as etiology of female passive smokers, e.g. ETS (environmental tobacco smoking), and high sensitivity to carcinogens via genetic and hormonal factors. Traditional epidemiological data showed controversial results of sex or gender differences in lung cancer susceptibility. Therefore, many confounders were expected in those studies. Among them, one is due to self reported smoking, therefore, the effects of ETS were not thoughtfully considered. The consequence of this is that there may be an overestimation of baseline lung cancer incidence or underestimation of the ORs in female smoke. To obtain undistorted mechanisms of etiology in side stream females, proper exposure monitoring of ETS and considering of susceptibility markers are recommended. For the exposure monitoring, urinary cotinine or naphthols are recommendable, while individual variation in their metabolisms, such as genetic polymorphisms in CYP2E1 and 2A6 and GSTT1 and GSTM1 among Asians should be understood. For susceptibility factors of lung cancer in passive smoker females, genetic polymorphisms of the CYP1A1 exon 7 Ile462Val and the GSTP1 exon 5 Ile105 Val are recommended. Sensitive to tobacco carcinogenic PAHs or CYP1A1 and ER cross talk are possible mechanism of the susceptibility biomarkers in the passive smoker females. (Cancer Prevention Res 10, 135-148, 2005)

내부표준물질, 9-hydroxyphenanthrene을 이용한 뇨 중 1-hydroxypyrene의 새로운 정량법

양미희,이호선,신민경,김용학 숙명여자대학교 약학연구소 2005 약학논문집-숙명여자대학교 Vol.22 No.-

Urinary 1-hydroxypyrene (1-OHP) has been used as exposure biomarkers for polycyclic aromatic hydrocarbons and analyzed with HPLC/FD after preparation by column extraction or simple centrifuge. However, the above methods have several drawbacks such as high noise or large volume of urine. In this study, we developed a new analysis method with liquid-liquid extraction and purification of commercial enzymes. We analyzed uirnary 1-OHP in 27 spot urines ofa subject in 3 weeks with the new method. As result, we could analyze all 27 samples clearly. The resolution of the new method was better than the previous centrifuge method. Therefore, the new method is a recommendable analysis method for urinary 1-OHP. Considering both of easiness and accuracy of the experiments, the new method should be further compared with the previous methods.

한국인에서 DPD 및 TS, MTHFR의 유전자다형

양미희 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.21 No.-

In-order to do efficient treatment of 5-Fluorouracil (5-FU) in Koreans, we investigated genetic polymorphisms of dihydropyrimidine dehydrogenase (DPD; gene, DPYD), thymidylate synthase (75), and methyltetrahydrofolate reductase (MTHFR), which are involved in 5- FU metabolism or efficacy in a Korean population. As results, we found genetic polymorphisms in DPYD-85, -1627, and -1896 site, TS-5'ER, and -3'U'TR, and MTHFR-222, 429 site among the Koreans (normal, N=105; head and neck cancer patients, N=28). 5-FU efficacy was determined into PR(partial response, decrease of cancer size over 50%) and SD (stable disease). Each or combination of the above 7 genetic polymorphisms were not associated with 5-FU efficacy. Therefore, the studied 7 genotypes can not be considered to result in individual variation in 5-FU efficacy.

CYP1A1 유전자발현과 소아천식의 상관

양미희 환경독성보건학회 2005 환경독성보건학회지 Vol.20 No.3

대학생의 결혼관에 대한 연구

양미희,권혜성 명지대학교 1986 家政學硏究 Vol.2 No.-

한국인의 고지혈증과 CETP 및 APOE 유전자다형

양미희,양지연,윤여정 숙명여자대학교 약학연구소 2006 약학논문집-숙명여자대학교 Vol.23 No.-

Since 1980s, hyperlipidemia in Korea has been rapidly increased and its related cardiovascular diseases are one of the main causes of death in Korea. Thus, for effective prevention of hyperlipidemia, clarification of etiology of the hyperlipidemia in Koreans is required. In this study, we focused on polymorphisms in genes, which are involved in cholesterol-transfer, and -binding, e.g. cholesteryl ester transfer protein (CETP) and apolipoprotein E (APOE). Study subjects were 257 Koreans (cases who had hyperlipidemia, LDL≥130, N=47; controls, N=210; Men, 62%). Genetic polymorphisms were determined by PCR-RFLP, and single base primer extension assays. As results, allele frequency of the CETP -Tag IB1 was significantly higher in cases than that in controls (p<0.05). When age and BMI were controled for statistics, there was 2.3 fold increase of OR (95%CI, 1.1-5.1) for hyperlipidemia in the Bl/Bl homozygotes compated to the B1/B2 heterozygotes. However, 2 SNPs, CI 12R and R158C of the APOE, or 5 haplotypes of the 2 SNPs were not associated with hyperlipidemia risk. Therefore, presence of the CETP-B1 allele has a potential of risk for Korean hyperlipidemia.

한국산 배(梨)를 중심한 과수의 기능성 연구

양미희 한국원예학회 2006 한국원예학회 학술발표요지 Vol.2006 No.5

In my previous study, consumption of Korean pears had inverse association with level of urinary 1-hydroxypyrene (1-OHP), which is an exposure biomarker and metabolite of polycyclic aromatic hydrocarbons (PAHs). Bioproduction of reactive intermediate of PAHs or induction of oxidative stress have been suspected as the mechanisms of PAHs-related diseases such as lung cancer, asthma, etc. Thus, to clarify potential of functional food in the Korean pears against PAHs-exposure, I studied effects of the Ko rean pears on 1. PAHstoxicokinetics, 2. PAHs- induced oxidative stress in intervention trials (N=30; male, N=10; female, N=20), and 3. PAHs- related chronic obstructive pulmonary disease (COPD: N= 30). The intake of Bulgogi, Korean barbeque, was used as exposure to PAHs. After 3 Ieks trials for the same subjects (1 week for Bulgogi only, Bulgogi with the pears, and the pears only, respectively), I found rapid excretion of urinary 1-OHP with the pears during 24 hrs compared to that without pears (0.05<p<0.1). In addition, I analyzed urinary levels of malondialdehyde (MDA), a biomarker for oxidative stress, following time cmyse. As results, I found that the Bulgogi induced MDA and the pears reduced the Bulgogi- induced MDA. I also analyzed another biomarker for oxidative stress due to PAHs, i.e. change in dichlorodihydro fluorescein (DCF). As a result, the DCF levels Ire not affected by intake of Bulgogi or the pears. HoIver, intake of the pear (half of a pear per day for 1 month) did not show significant improvement of ‘the St George’s respiratory questionnaire score’ or ‘forced expiratory volume for 1 second (FEV). In conclusion, my results suggest that the Korean pears have chemoprevention effects on PAHs- induced carcinogenic mechanisms due to rapid excretion of PAHs- intermediates and reduction of PAHs- induced oxidative stress. However, the pear-chemopreventive effects against PAHs-related diseases should be further clarified in enlarged population studies.