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단감에서 Deltamethrin의 안전사용기준 설정을 위한 잔류량 평가
심재한,이강봉,서용택,양광록 全南大學校 農漁村開發硏究所 1990 農業科學技術硏究 Vol.25 No.-
Deltamethrin의 단감 재배기간중 살포횟수 및 처리시기에 따른 잔류수준을 시험하여 다음과 같은 결과를 얻었다. 1. Deltamethrin의 잔류분석 회수율은 단감시료에 대해 0.1ppm과 1.0ppm에서 각각 평균 90.20%, 90.73%를 보였고 검출한계는 0.01ppm이었다. 2. Deltamethrin의 단감에서의 잔류는 Mills 등의 방법으로 분석한 결과 수확 3일전 6회 처리구에서만 0.16ppm으로 잔류 허용기준을 초과하였고 이로 미루어 deltamethrin의 단감중 안전사용은 수확 3일전 5회 이내의 사용으로 추천할 수 있었다. The effects of application number on the persistence of deltamethrin in sweet persimmons was analyzed. Deltamethrin EC(1%) was sprayed on the sweet persimmon trees at regular intervals (400 L/10a, 1,000 times diluted deltamethrin EC was sprayed on 60th, 45th, 30th, 15th, 7th, 3rd days before harvest). The procedure of residue analysis was based on the multiresidue analytical method using a polarized acetonitrile solution as the extracting solvent. Residue of deltamethrin were determined by alternate eluate (acetonitrile, hexane, dichloromethane 1.5 : 48.5 : 50 v/v) from the Florisil column to gas chromatographic analysis (ECD). Residue level of deltamethrin in sweet persimmons were proved to 0.16 ppm when the deltamethrin EC (1%) was sprayed 3 days before harvest. The recoveries were above 90% in spiked sweet persimmon samples, regardless of concentration.
심재한,서용택,양광록 한국농화학회 1991 Applied Biological Chemistry (Appl Biol Chem) Vol.34 No.4
현재 많이 사용되고 있는 농약 7종을(IBP, Isoprothiolane, Ridomil, Cartap, Chlorothalonil, Captafol, Endosulfan) 교호적으로 혼합하여 농약들의 협력작용이 잉어에 미치는 영향과 각 농약들의 LC_50치와 협력작용에 의한 synergism ratio(SR)를 조사하여 다음과 같은 결과를 얻었다. 7종 농약의 LC_50은 IBP(10.06 ppm), Isoprothiolane(9.67 ppm) Ridomil(>40.00 ppm), Cartap(0.67 ppm) Chiorothalonil(0.078 ppm) Captafol(0.05 ppm), Endosulfan(0.0074ppm)이었고 7종 농약들을 교호적으로 혼합하여 synergism의 유무로 결정하였다. 그 결과 Isoprothioane과 IBP, Isoprothioane과 cartap에서 synergism이 있는 것으로 판명되었고 이들의 synergism LC_50은 각각 5.23ppm(Isoprothiolane-IBP), 6.30ppm(Isoprothiolane+Cartap)으로 나타났으며 이것을 synergism ratio에 나타내면 Isoprothiolane+lBP에서는 1.85이고 Isoprothiolane + Cartap에서는 1.53이었다.
농약 상호간의 협력작용에 의한 잉어의 독성과 해독효소 활성의 비교
서용택,심재한,양광록 한국농화학회 1992 Applied Biological Chemistry (Appl Biol Chem) Vol.35 No.5
This study was performed to investigate effects of probable detoxifying enzyme activity and toxicity by pesticides and their combinations in the fresh water fish. Seven pesticides including IBP, isoprothiolane, cartap, ridomil, chlorothalonil, captafol and endosulfan were subjected to investigate for their acute toxicites and synergism possibilities. The LC_(50) value of endosulfan was the lowest at showing 0.0079 ppm and that of metalaxyl was the highest as showing 40 ppm over. The synergism effects of relative pesticides were observed in the combinations of isoprothiolane+IBP and isoprothiolane+cartap. The changes of glycogen contents in fish liver were assayed for 5 pesticides and its highest inhibition effect of glycogen showed in IBP treated fish. The activity of probable detoxifying enzymes including carboxylesterase (CE), glutathion S-transferase (GST) and lactate dehydrogenase (LDH) were assayed in carp liver at dose of sublethal concentrations. Effects of pesticides on changes in each enzyme activities were as follows: carboxylesterase (CE) activities were the highest in IBP and glutathion S-transferase (GST) activities were the highest in iosoprothiolane+IBP. Both activities of carboxylesterase (CE) and glutahtion S-transferase (GST) were increased by 5 chemicals. The highest LDH activity showed in isoprothiolane treated fish, while the lowest activity was observed in isoprothiolane+cartap. Sublethal exposure to cartap and isoprothiolane+cartap in carp exerted various effects on LDH activity.