인태아 하요척관절의 관절원판 발육에 관한 연구

안호범(Ahn Ho Beom),윤재룡(Yoon Jae Rhyong) 대한체질인류학회 1993 대한체질인류학회지 Vol.6 No.1

하요적관절은 상요적관절과 같이 회내 회외운동을 하며 관절원판은 하요적관절과 요골수근관정 사이에 존재하고 있다 관절원편은 하요척관절의 안정을 유지시키는데 중요한 구조로서 섬유대 또는 섬유연골대가 적고 경상돌기에서 요골하단부내연을 연결하고 있다. 수근관절의 발생에 관해서는 양은 연구보고가 있으나 하요적관절 발육에 관한 보고는 드물고 관정원판의 선자현미경석 관찰은 없는 듯 하다. 저자는 좌고 30mm 부터 260mm 까지의 인태아를 대상으로 하요적관절과 관절원판의 발육과정을 광학 및 전자현미경적 관찰로 다응과 같은 결과를 얻었다. 좌고 40mm(태령 10주 ) 태아때 하요척관절과 요골수근관절의 원시강이 줄현하였고 100mm( 태 령 15주 ) 태아때는 관절강의 완성을 관찰하였다. 관설원판의 원기는 좌고 40mm 태아때 미분화 간엽세포의 밀집대로 줄현하였고 이세포에는 대량의 유리리보솜과 소량의 조연내형절망 사립제 및 골지복합제를 함유하고 있었다. 좌고 100mm( 태령 15 주 ) 태아때 관절원판의 외측부에는 방주상의 섬유모세포들이 치밀하게 배열하고 있었고 중간부에는 작은 원형의 섬유모세포들이 소생으로 배열하고 있었다. 좌고 150mm( 태령 19 주 ) 태아때 관절원판의 섬유모세포의 크기가 증대되었고 교년 섬유는 미량 존재하고 있였다. 좌고 200mm ( 대령23주) 부터 260mm (태령 30주) 태아까지의 관철원판 외즉부에는 섬유모세포와 다량의 교원섬유블이 출현하였고 중간부에는 섬유모세포와 교원섬유가 엉켜 존재하고 있었다. 이와같은 결과로 미루어 인태아 하요적관절의 관절원판은 태생기에 섬유모세포로 존재하고 생후에 섬유연골세표로 분화 발육하리라 생각된다.

인태아 수지굴근건의 발육에 관한 전자현미경적 연구

윤재룡,안호범,남광일,Yoon, Jae-Rhyong,Ahn, Ho-Beom,Nam, Kwang-Il 한국현미경학회 1996 Applied microscopy Vol.26 No.2

The development of flexor digital tendon of the hand was studied by electron microscopy in human fetuses ranging from 9 mm to 260 mm crown rump length. The primordium of tendons was first identified as discrete collection of mesenchymal cells at 25 mm fetus. Synovial sheath formation had commenced by 40 mm fetus and was complete by 70 mm fetus. Cell junction or adhesion sites at all ages were noted between the tendon cells. When dilatation of the synovial cavity occurred, two types of synovial cells were observed. A-type cells had numerous vesicles and large vacuoles. In contrast, B-type cells were characterized by abundant rough endoplasmic reticulum and well-developed Golgi complex. By $150mm{\sim}260mm$ fetuses, a mojority of the synovial cells were type B. The most remarkable difference between the synovial cells of full-term fetus and adult was the larger amount of collagen fibers in the latter. The vascular buds were first observed between the individual fibril bundles in the interfascicular space at 150 mm fetus. At 25 mm fetus, collagen fibrils were first noted within narrow cytoplasmic recesses which were continued with the extracellular space. Collagen fibrils were filled in almost entire extracellular space at 150 mm fetus. Besides collagen fibrils in the extracellular space small elastic fibers were also identified and followed in their development.

기저세포암 및 편평세포암에서 미세혈관밀도와 p53 단백 발현에 관한 연구

어수락,조규성,안호범,김대영,이삼용,조백현 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

Basal cell carcinoma(BCC) and squamous cell carcinoma (SCC) are very prevalent neoplasms of the human skin. Ultraviolet radiation in sunlight is a well-established mutagen of the p53 gene and is one of basal cell carcinoma and squamous cell carcinoma. The newly-formed vascular network is important for neoplasms to grow beyond a size of about 1 ㎣. Recent reports have suggested the hypothesis that a mutant p53 protein is closely related with capillary density. Immumohistochemistry for p53 protein and CD34 was performed in 20 cases of BCCs and 14 SCCs to evaluated the relationship between p53 protein and capillary density. The results were as follows:1. The microvessels stained by CD34 were mainly located in the interface of tumor cells and stroma. 2. There was no difference in the microvessel density according to the histologic types and age of the patients, but a higher microvessel density was noted in male patients. 3. The aggressive BCCs and the less-differentiated SCCs showed higher p53 immunostaining. 4. The mean microvessel density of cases showing strong positive immunostaining of the p53 gene(54.73±17.75) was higher than that of others(39.75±18.30). These results suggested that p53 protein expression and microvessel density are not related to the histologic types and age of the patients, but that differentiation and biologic behavior such as the infiltrating property of tumors and the microvessel density are closely related to p53 protein expression.

개방창 감염에 대한 Flomoxef의 임상 효과

윤강모,안호범,김대영,이삼용,조백현,이민철 최신의학사 1995 最新醫學 Vol.38 No.5

코뼈 골절의 전산화 단층 촬영에 의한 분류

노복균,안호범,김대영,이삼용,조백현 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.2

The reduction of nasal bone fracture has been done by a simple procedure. The nasal bone fracture was not a serious problem and patients are usually treated as outpatients. However it is causing increased demand for medical services far cosmetic reasons. We studied the nasal bone fracture by retrospective analysis, it comprising 606 patients with nasal bone fractures in various accidents and treated of Chonnam University Hospital from March 1995 to February 1998. We reviewed and analyzed the medical records and facial bone CT scans of 606 patients. The following results were obtained. 1. The prevalent age group was in the third decade, the most common cause was traffic accidents, while nasal swelling, tenderness, nasal deviation and crepitation were examined 2. The classification of nasal bone fracture was done by facial bone CT. Class 1: Nasal tip depressed fracture(16%) Class 2: Displaced nasal bone fracture without depression(30%) Class 3: Displaces nasal bone fracture with depression(12%) Class 4: Comminuted nasal bone fracture (10%) Class 5: Simple fracture of nasal bone and frontal process of maxilla(14%) Class 6: Comminuted fracture of nasal bone and frontal process of maxilla(11%) Class 7: Nasal bone fracture without displacement(9%)

횡복직근피판을 이용한 치험례

김대영,이삼용,안호범,조백현,류봉수,윤강모 大韓成形外科學會 1995 Archives of Plastic Surgery Vol.22 No.2

Adequate soft tissue coverage is a primary requisite for the reconstruction of the large soft tissue defect of body. The rectus abdominis muscle is long and flat and it can be used as transverse or vertical myocutaneous flap. The TRAM flap is useful for moderate sized defects requiring a muscle or well vascularized tissue between Gracilis and Latissimus dorsi in size. We have experienced 6 cases of large soft tissue defect of the body using transverse rectus abdominis myocutaneous free flap. Advantages of this method are:enough available donor tissue, effect of abdominolplasty, no deformity of the donor site, hidden donor site scar, and blood supply by two arteries. Six patients showed successful results except one case of flap necrosis. The flap necrosis was due to hematoma around the vascular anastomotic site. We think reconstruction with TRAM flap is appropriately available for moderate sized soft tissue defect.

피부 편평상피암종과 기저 세포암종에서의 Rb 단백의 발현

조상윤,이삼용,안호범,조백현,김대영 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.2

There is increasing evidence that inactivation of tumor-suppressor genes can promote tumor growth. Retinoblastoma protein (pRb) is the product of the retinoblastoma gene located on chromosome 13q14. pRb negatively regulates cell growth when functioning normally. Mutational inactivation of the Rb gene has been observed in retinoblastomas, osteosarcomas and soft tissue sarcomas. Recently, several other human cancers have also been shown to carry abnormalities of the Rb gene. The potential role of the Rb gene in cutaneous squamous cell carcinomas (SCCs) and basal cell caicinomas (BCCs), has not been determined and was the focus of this study. Immunohistochemical expression of pRb in 16 cutaneous SCCs and 17 BCCs was examined. The expression of PCNA was studied in parallel to assess the cellular proliferation rate in these lesions. The pRb and PCNA immunoreactivity were localized to the nuclei of tumor cells. A few pRb and PCNA positive cells were seen in normal squamous epithelium, sebaceous glands, sweat glands and hair follicles. The loss of expression of pRb was seen in 3 of 16 SCCs(18.8%) and 6 of 17 BCCs (35.3%). PCNA immunoreactivity was slightly high in pRb-negative or lower-positive cases. PCNA immunoreactivity was similar to that produced by pRb in some cases. These results suggest that mutational inactivation of the Rb gene may be related to the carcinogenesis of cutaneous SCC and BCC, though the frequency is relatively low.

정맥 주사의 혈관외 유출로 인한 조직 손상의 임상적 고찰

김대영,이삼용,안호범,조백현,류봉수,김광석 大韓成形外科學會 1995 Archives of Plastic Surgery Vol.22 No.2

The intravenous extravasation of maintenance fluids (0.9% saline, 5% D/W, 5% D/S, 10% D/W, Hartmann is solution), Calcium gluconate, KCl solution, chemotherapeutic agents may cause local or systemic symptoms. Tissue might be injured by these agents. Five possible mechanisms to tissue damage should be considered:1) osmotic damage, 2) ischemia secondary to impaired circulation, 3) direct cellular toxicity, 4) mechanical compression, and 5) infection. At the extravasation injuries, appropriate diagnosis and treatment are important. The therapeutic method varies in injured tissue from conservative treatment or simple wound care to flap surgery. But the most important measurement is to prevent the extravasation injuries. The intravenous extravasation of electrolyte or chemotherapeutic agent can cause tissue damage, the damage tissue is healed with scar or functional deformity of joint (94%). The damaged tissue by fluid is healed without scar or functional deformity. We have done a retrospective study of twenty-four patients with major intravenous extravasation injuries analyzed in terms of age, sex, causative agents, natural history, method of treatment and results.