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한천 분해균(Cytohaga sp. ACLJ-18)이 생산하는 agarase의 정제 및 특성
주동식,송해미,이정석,조순영,이응호 한국생물공학회 1998 KSBB Journal Vol.13 No.3
Agar degrading enzyme-agarase-was purified from the culture fluid of Cytophaga so/ ACLJ-18, by acetone precipitation, DEAE-Cellulose, Sephadex G-100 and CM-Sephadex C25 column chromatographies. The molecular weight of purified agarase was estimated to be 24,700 dalton by SDS-polyacrylamide gel electrophoresis. The optimum pH and temperature for agarase activity were 7.0 and 40$^{\circ}C$, respectively. this agarase was stable in the pH range of 6.5 - 8.0 and 40$^{\circ}C$, and required 0.35M NaCl for optimum activity. And this agarase was inhibited by metal ions such as Ba2+, Cu2+, Co2+, Mn2+, Hg2+, Zn2+, and showed specificity on agar.
수용성 키토산 유도체를 이용한 방사성 스트론튬의 시험관내 흡착능 및 마우스내 체외배출 효과
김희경,송해미,조덕제,조만기,이정석,이응호 동서대학교 부설 연구소 1996 연구소 논문집 Vol.1 No.-
키토산은 천연착화제로서 효용가치가 높은 무독성의 천연고분자로서 키틴으로부터 탈아세틸화시킨 유도체이다. 키토산에 인산기을 도입한 Water-solubel phosphorylated Chitosam(WSPC)의 Sr에 대한 시험관내 흡착률은 pH 3에서는 97.8%이었으나, pH 5와 pH 7에서는 모두 100%이었다. WSPC를 농도별(0.01%, 0.1%, 1%)로 희석하여 11주령의 ICR 수컷 마우스의 위내로 투여한 직후 Sr을 같은 경로로 처리한 다음, WSPC의 Sr에 대한 체외 배출효과와 골내 잔존률을 평가하였다. 또한 Sr와 농도별 WSPC 처리후 6일째에 척추, 두개골, 대퇴골, 경골, 치아 및 꼬리뼈 등에 대해 Sr 잔존율을 측정하였다. 0.01-0.1% 사이의 WSPC를 접종한 마우스 투여군이 Sr만 경구 투요한 대조군에 비하여 골조직에 잔존하는 Sr의 침착률이 낮게 나타났다.(P<0.01). 또한 방사성 스트론튬의 분뇨를 �蔥�배출 효과 역시 WSPC 처리군이 대조군에 비해 유의성이 인정되었고(P<0.05), 대체로 WSPC 농도가 높을수록 그 효과는 비례하였다.(P<0.05). 본 실험에 사용된 WSPC는 체내에 유입된 Sr을 제거하는데 효과가 인정되어 방사성 스트론튬의 방어약제중 하나로 활용할 수 있다고 판단되었다.
수용성 키토산 유도체를 이용한 방사성 스트론튬의 시험관내 흡착능 및 마우스내 체외배출 효과
김희경,이정석,송해미,조만기,조덕제,이응호 한국키틴키토산학회 1997 한국키틴키토산학회지 Vol.2 No.1
The purpose of this study is to evaluate the effect of water-soluble phosphorylated chitosan(WSPC) on the absorption and the excretion of radiostrontium(85Sr). By the analysis of column chomatography, the absorption of 85Sr to WSPC was 100% at PH 5 and 7(25℃),and 97.8% at pH 3(25℃). For evaluting the excretive effect of WSPC to 85Sr, 11-week old male ICR mice were used and divided into four groups; group A was only treated with 85Sr(0.2yci/head) as controls, and the other groups administrated with 0.01%(B), 0.1%(C), 1%(D) WSPC(1ml/head) immediately after the inoculation of 85Sr (0.2yCi/head) by intragastric route, respectively. Five mice in each group were allocated and were periodically collected their feces and urines from 1 hour to 6 days after the inoculation for examining the quantity of 85Sr. At 6 days of postinoculation(pi), all mice were sacrificed and were sampled from 6 bones(spine, skull, femur, tibia, tooth and tail) for counting the radioactivity of 85Sr. Fecal and urinary excretion of 85Sr was significantly higher in groups B, C and D compared with that in group A during 24 hours pi(P<0.05). Among them group D showed the highest excretive effect in feces and urines than groups B and C (P<0.05). In addition, bony retention of 85Sr in groups B, C and D were significantly lower than that in group A (P<0.01). In conclusion, we suggest that WSPC could be used as an effective agent for the inhibition of uptake and promotion of excretion of 85Sr from animals as well as human beings.