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형질전환 벼 현탁세포를 이용한 hCTLA4Ig 생산에서 proline과 gelatin이 미치는 영향
송미나(Mi-Na Song),전수환(Su-Hwan Cheon),권준영(Jun-Young Kwon),최성훈(Sung-Hun Choi),김동일(Dong-Il Kim) 한국생물공학회 2009 KSBB Journal Vol.24 No.3
본 연구에서는 형질전환된 벼 세포를 이용하여 자가면역 질환의 치료제인 human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig)을 생산하였고, RAmy3D promoter와 RAmy1A signal peptide를 사용하여 당 고갈시에 목적 단백질의 발현과 배지로의 분비를 유도하였다. 이 시스템의 문제점은 당 고갈에 의해 목적 단백질의 생산이 유도되기 때문에 에너지원이 없어 세포의 사멸, 이로인한 배지내로의 protease 분비를 유발하게 된다. 이것은 목적 단백질의 안정성 저해와 궁극적으로는 생산성의 손실을 일으킨다. 따라서 세포를 보호하여 사멸을 막는 효과가 있음이 보고된 proline을 첨가하여 생산성 증진 효과를 확인하고자 하였다. 4 mM의 proline을 첨가하여 배양하였을 때, 세포의 사멸 및 protease의 분비를 줄일 수 있었고 이는 결과적으로 hCTLA4Ig의 생산성 증진으로 이어졌다. 또한 단백질 안정제를 통하여 배지내로 분비된 hCTLA4Ig 의 안정화를 이루어 생산성을 높이고자 하였다. 0.01 g/L 의 gelatin을 적용하여 생산성 증진 효과를 확인하였으며, 이는 hCTLA4Ig의 안정화에 의한 것임을 알 수 있었다. Protease의 분비를 줄이기 위한 세포 보호와 protease의 공격을 막기 위한 hCTLA4Ig 보호를 통하여 형질전환된 벼 세포를 이용한 hCTLA4Ig의 안정성 및 생산성을 증대 시킬 수 있음을 확인하였다. Rice cells were transformed to express human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) using RAmy3D promoter. hCTLA4Ig was produced and secreted into culture media inducibly when sugar was depleted. The obstacles of this system are the cell death and release of proteases by sugar starvation. These problems resulted in the losses of stability and productivity of hCTLA4Ig. Therefore, the effects of proline as an inhibitor of cell death were investigated. When 4 mM proline was added in sugar-free media, the cell death and release of proteases were reduced. As a consequence, the production of hCTLA4Ig was enhanced. In addition, the effects of protein stabilizers such as gelling agents were studied. It was found that the application of 0.01 g/L gelatin led to an increase in hCTLA4Ig production. This increase might be originated from the stabilization of hCTLA4Ig. In conclusion, the production of hCTLA4Ig could be enhanced by the additions of proline and gelatin in transgenic rice cell cultures.
프로테오믹스 기술을 통한 쥐의 신장 피질에서 알도스테론(Aldosterone)에 의해 조절되는 단백질 동정
송미나(Mi-Na Song),전홍배(Hong-Bae Jeon),최효정(Hyo-Jung Choi),권태환(Tae-Hwan Kwon),백문창(Moon-Chang Baek) 대한약학회 2010 약학회지 Vol.54 No.3
Aldosterone, mineralocorticoid hormone, has important functions related to the regulation of blood pressure and balance of fluids and electrolytes in the distal region of the nephron. By genomic and non-genomic action of aldosterone, the physiological kidney functions are modulated. However, many of them except several kind of sodium channel have not been identified and analyzed yet. In this study, proteomic technologies with two-dimensional gel electrophoresis (2-DE) gel using aldosterone rat model were applied to analyze and identify the aldosterone dependently expressed proteins in rat kidney cortex. As a result, the established aldosterone rat model exhibited the normal physiological responses to aldosterone and modulated proteins were identified, which included 15 increased and 3 decreased proteins on 2-DE analysis. Among them, 11 proteins were identified as changed proteins by LC-MS/MS analysis. These proteins identified as aldosterone induced proteins were involved in several cellular pathways such as cytoskeleton remodeling, energy metabolism, amino acid metabolism, and chaperone process. In conclusion, our data could provide the insights into the new mechanism underlying regulation of kidney functions by aldosterone.
성문상부암종에서 성문상 후두부분절제술과 경부청소술의 치료성적
태 경(Kyung Tae):민현정(Hyun Jung Min),송미나(Mi Na Song),신광수(Kwang Soo Shin),이승환(Seung Hwan Lee),김경래(Kyung Rae Kim),이형석(Hyung Seok Lee) 대한두경부종양학회 2007 대한두경부 종양학회지 Vol.23 No.1
Background and Objectives :Supraglottic partial laryngectomy is oncologically sound surgical procedure for selected cases of laryngeal cancer which maintains physiologic speech and swallowing without permanent tra-cheostoma. The purpose of this study is to evaluate the oncologic and functional results of supraglottic partial laryngectomy and neck dissection for supraglottic cancer. Materials and Methods :Between 1991-2005, Twenty-three supraglottic cancer patients, underwent supra-glottic partial laryngectomy, were studied retrospectively. There were 5 patients with cT1, 14 with cT2, 4 with cT3 and 11 patients with cN0, 1 with cN1, 10 with cN2, 1 with cN3. All patients underwent neck dissection and postoperative radiotherapy was added to twenty patients. They were reviewed with respect to primary subsites, extended subsites, treatment result, survival rate, factors affecting the prognosis, postoperative complication, time of decannulation and oral diet, and postoperative voice. Results :Among eleven patients with clinically negative node, six patients had pathologically positive nodes. So occult metastasis was 54.5%. Two patients recurred at cervical lymph node and one had distant metastasis to lung. Local and regional control were 100% and 91.3%. The overall 3-year and 5-year survival rate were 84%, 78%, respectively. Nineteen cases were squamous cell carcinomas and four were basaloid squamous cell carcinomas. Basaloid subtype was significantly affected to survival. Decannulation and oral feeding were possible in 100%. Conclusions :Supraglottic partial laryngectomy is oncologically safe and functionally good procedure in supraglottic cancers. Elective neck dissection is beneficial in management of occult cervical metastasis.