http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
양식 넙치(Paralichthys olivaceus)에 경구투여한 Ampicillin 및 Amoxicillin의 어류체내 잔류량의 변화
손광태 ( Kwang Tae Son ),조미라 ( Mi Ra Jo ),오은경 ( Eun Gyoung Oh ),목종수 ( Jong Soo Mok ),권지영 ( Ji Young Kwon ),이태식 ( Tae Seek Lee ),송기철 ( Ki Cheol Song ),김풍호 ( Poong Ho Kim ),이희정 ( Hee Jung Lee ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.5
Residues of ampicillin and amoxicillin in the muscles of olive flounder Paralichthys olivaceus were investigated after oral doses of 20 mg/kg body weight/day of ampicillin and 40 mg/kg body weight/day of amoxicillin in a first examination and 40 mg/kg body weight/day of ampicillin and 80 mg/kg body weight/day of amoxicillin in a second examination for 5-6 days. The ranges of seawater temperatures in the 1st and 2nd examinations were 13.4-15.6oC and 16.8-21.4oC, respectively. Ampicillin and amoxicillin concentrations were analyzed by high performance liquid chromatography (HPLC). The average recovery rates of ampicillin and amoxicillin in fish samples ranged from 84.8- 95.2% and 100.8-103.8%, respectively. Residual concentrations in the olive flounder peaked on the 5th day, with average concentrations of 0.075 mg/kg and 0.311 mg/kg in the 1st examination, and 0.098 mg/kg and 0.630 mg/kg in the 2nd examination for ampicillin and amoxicillin, respectively, with maximum concentrations in muscle. In the 1st examination, ampicillin concentrations in olive flounder after withdrawals of 1 and 3 days were 0.041 mg/kg and 0.023 mg/kg, respectively, and amoxicillin concentrations after withdrawals of 1 day and 10 days were 0.172 mg/kg and 0.023 mg/kg, respectively. In the 2nd examination, the ampicillin concentrations in olive flounder after withdrawals of 1 and 3 days were 0.041 mg/kg and 0.023 mg/kg, respectively, and amoxicillin concentration after withdrawals of 1 day and 10 days were 0.172 mg/kg and 0.023 mg/kg, respectively. We suggest that the recommended withdrawal periods should be 3 days for ampicillin and 10 days for amoxicillin in the olive flounder.
유통 마른 김 (Porphyra sp)의 중금속 (Hg, Pb, Cd) 함량과 위해성 평가
손광태 ( Kwang Tae Son ),권지영 ( Ji Young Kwon ),조미라 ( Mi Ra Jo ),최우석 ( Woo Seok Choi ),강성림 ( Sung Rim Kang ),하나영 ( Na Young Ha ),신진월 ( Jin Wall Shin ),박큰바위 ( Kunbawui Park ),김지회 ( Ji Hoe Kim ) 한국수산과학회(구 한국수산학회) 2012 한국수산과학회지 Vol.45 No.5
To investigate heavy metals (Hg, Pb and Cd) and their potential health risks in commercial dried layer (Porphyra sm), we collected 45 samples from the major production areas on the western and southern coasts of Korea (Hwaseong, Seo-cheon, Gunsan, Muan, Shinan, undo, I-laenam, Wando, Jangheung, Goheung and Busan). The Hg, Pb and Cd concentrations were measured using inductively coupled plasma spectrometry (ICP-MS) or a mercury analyzer, The average Hg, Pb and Cd concentrations in the dried layer were 0.006±0, 0.017, 0.196+0.0614 and 0.894+0.4882 mg/kg, respectively, Based on the 2007 Korean Public Nutrition Report, these levels are 0.02, 0.11 and 2.47% of the provisional tolerable weekly intake (PTWI) for Hg, Pb and Cd, respectively, established by the FAO/WHO. The hazard quotient (HQ) determined from the ratio of exposure and safe levels were less than 1.0. Therefore, the levels of overall exposure to Hg, Pb and Cd for dried layer were below the recommended JECFA (Joint FAO/WHO Expert Committee on Food Additives) levels, which indicate safe levels for public health.
굴(Crassostrea gigas)의 선도에 따른 pH, 글리코겐, 가용성단백질, 탁도와의 상관관계
손광태 ( Kwang Tae Son ),심길보 ( Kil Bo Shim ),임치원 ( Chi Won Lim ),윤나영 ( Na Young Yoon ),서정화 ( Jeong Hwa Seo ),정삼근 ( Sam Geun Jeong ),정우영 ( Woo Young Jeong ),조영제 ( Young Je Cho ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.5
We examined chemical changes in oysters Crassostrea gigas and packing water that were sold after storage at 5, 10, and 20℃. The pH of oysters stored at 5℃ dropped to 5.81 after 10 days of storage, while that of oysters at 10℃ and 20℃ dropped to 5.37 after 8 days and to 5.04 after 4 days, respectively. The glycogen content of oysters stored at 5℃ decreased from 718.89 to 421.85 mg/100g during storage, while that of oysters at 10℃ decreased to 351.49 mg/100 g after 4 days. The turbidity and soluble protein in packing water increased slightly. The viable cell count of oysters did not exceed 6 log CFU/g after 10 days of storage at 5℃, but that of oysters at 10℃ did so after 8 days. Additionally, the viable cell count of packing water was lower than that of oysters. We performed a principal component analysis, where the first principal component (55.03%-57.24%) and second principal component (42.76%-44.97%) described most variation. The first principal component included the pH of oysters and packing water, and the glycogen content of oysters. A Pearson correlation between the first two principal components had a higher R value than that between other components. Freshness was evaluated using the pH of oysters and packing water, and glycogen. We found that soluble protein content was significantly associated with a lower pH and glycogen content.
넙치 , 범가자미 및 잡종 ( 넙치 ♀ × 범가자미 ♂ ) 근육의 식품화학적 특성 비교
김태진(Tae Jin Kim),민진기(Jin Gi Min),윤호동(Ho Dong Yoon),이두석(Doo Seog Lee),박정흠(Jeung Hum Park),손광태(Kwang Tae Son),김경길(Kyung Gyl Kim) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.1
The proximate composition, mineral content, fatty acid composition, ATP related compounds, amino acid compositon, color and texture were investigated with dorsal and ventral muscle from olive tlounder, spotted flounder and hybrid (olive flounder ♀ × spotted flounder ♂). Spotted flounder and hybrid were higher in moisture content, and lower in crude protein content than those of olive flounder. Patassium content in hybrid was higher than that in olive flounder and spotted flounder. Hybrid was lower in calcium, iron, manganese content, and higher in magnesium content than olive flounder and spotted flounder. The contents of saturated fatty acid, unsaturated fatty acid and docosahexaenoic acid (DHA) in hybrid were intermediate level of spotted flounder and olive flounder. Fatty acid composition of dorsal muscle was slightly similar to ventral muscle. Adenosine triphosphate (ATP) and its related compounds contents and amino acid content in hybrid were intermediate level of spotted flounder and olive flounder, and these compounds of dorsal muscle were slightly similar to those of counterpart. The major amino acids such as aspartic acid, glutamic acid, leucine and lysine were very similar to all the samples. Total amino acid and essential amino acid contents in dorsal muscle were slightly higher than those in ventral muscle. Free amino acid content and composition in hybrid were similar to spotted flounder, and free amino acid content in dorsal muscle was higher than in ventral muscle. The lightness of hybrid and spotted flounder was stronger than that of olive flounder, and was stronger in dorsal muscle. The breaking strength of hybrid was slightly lower than that of spotted flounder, and was stronger in dorsal muscle.
어류 중 4계열 잔류 항생물질 검출을 위한 Lateral Flow Immunoassay Kit 개발
조미라 ( Mi Ra Jo ),손광태 ( Kwang Tae Son ),권지영 ( Ji Young Kwon ),목종수 ( Jong Soo Mok ),박홍제 ( Hong Jae Park ),김현용 ( Hyun Yong Kim ),김경동 ( Gyung Dong Kim ),김지회 ( Ji Hoe Kim ),이태식 ( Tae Seek Lee ) 한국수산과학회(구 한국수산학회) 2015 한국수산과학회지 Vol.48 No.2
A lateral flow immunoassay kit based on antigen-antibody interactions was developed to detect residues of beta-lactams, quinolones, tetracyclines, and sulfonamides in farmed fish. Group-specific antibodies showing cross-reactivity with other antibiotics in the same group were produced in rabbits. The rabbits were immunized eight times to obtain the maximum titers. Antibodies were extracted from the antisera collected from the immunized rabbits and produced group-specific reactions with antibiotics from the four groups. A kit was prepared that optimize conditions for the antigen-antibody reaction, using colloidal gold conjugated antibodies, and was designed to detect the four groups of antibiotics simultaneously. The kit enabled the detection of antibiotics in the four groups at below maximum residue limits (MRLs), which were 200 μg/kg for tetracyclines, 100 μg/kg for sulfonamides, 50 μg/kg for beta-lactams, and 100 μg/kg for quinolones. The cross-reactivity of the antibodies ranged from 10-80% for the sulfonamides, 20- 100% for tetracyclines, 38-100% for quinolones, and 20-100% for the beta-lactams, confirming that the antibodies were group specific. The test kit was used 30 times to examine spiked antibiotics at the limits of detection (LODs) and all produced positive results, indicating high sensitivity. The LODs for the assay ranged from 4-20 ng/mL for beta-lactams, 25-50 ng/mL for sulfonamides, 20-100 ng/mL for tetracyclines, and 30-80 ng/mL for quinolones, and there were no false negative reactions at above these LODs. In addition, all of the LODs of the developed kit were correlated with high-performance liquid chromatography (HPLC) data. Our lateral flow immunoassay kit can simultaneously detect antibiotic residues from a large number of fish samples rapidly, strengthening the safety of domestic farmed and imported fish.
산 처리에 의한 파래(Enteromorpha sp.)의 유해 중금속 제거
목종수 ( Jong Soo Mok ),손광태 ( Kwang Tae Son ),이태식 ( Tae Seek Lee ),권지영 ( Ji Young Kwon ),박큰바위 ( Kunbawui Park ),김지회 ( Ji Hoe Kim ) 한국수산과학회(구 한국수산학회) 2017 한국수산과학회지 Vol.50 No.1
The elimination of harmful heavy metals (Cd, Cr, and Pb) from sea lettuce Enteromorpha sp. was evaluated in filtered seawater over a pH range of 2.0-4.0 using citric, hydrochloric, and nitric acids. We also evaluated the quality of sea lettuce samples after release of their internal constituents into seawater solutions containing acids. The heavy metals that accumulated in raw sea lettuce after incubation for 3 days in seawater containing Cd, Cr, and Pb were, in descending order, Pb (120.45 μg/g), Cr (86.04 μg/g), and Cd (18.35 μg/g). The rate of elimination of heavy metals from sea lettuce was higher at lower pH for all of the acids used. However, the color of the sea lettuce changed adversely at below pH 2.5. The heavy metals in sea lettuce samples after 10 min in seawater at pH 3.0 containing the three acids were eliminated in the order Pb (42.2-78.0%), Cd (51.8-55.3%), and Cr (14.0-32.8%). The quality of the sea lettuce was not affected when it was incubated for 30 min at pH above 3.0. The maximum elimination of heavy metals from sea lettuce occurred when it was soaked for 10 min in seawater at pH 3.0 containing citric acid.