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범가자미 , Verasper variegatus 수컷에서 estradiol-17β 에 의해 유도된 vitellogenin 의 면역학적 특성
김윤(Yoon Kim),김우진(Woo Jin Kim),백혜자(Hea Ja Baek),김경길(Kyung Gyl Kim),방인철(In Chul Bang),한창희(Chang Hee Han) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.3
Vitellogenin(Vg) was purified from plasma of estradiol-17β(E₂)-treated spotted flounder, Verasper variegatus, by precipitation with cold distilled water, followed by fractionation using a Sepharose CL-6B column chromatography. E₂-induced protein was identified as Vg by SDS-PAGE and western blot analysis. The molecular weight of the purified Vg was estimated 175kD as determined by SDS-PAGE. In order to measure the Vg level, monoclonal antibodies against Vg were produced by hybridoma technique. Purified Vg was immunized into Balb/c mice and then the spleen cells from mice were fused with NS-1 myeloma cells. The hybridoma cells were screened by enzyme-linked immunosorbent assay(ELISA) and subcloned by limiting dilution. The hybridoma clones which secreted antibodies highly reactive to the purified Vg were designated as 4D6. Its specificity was demonstrated by western blot from plasma of untreated, E₂-treated mate fish, and purified Vg.
김태진(Tae Jin Kim),윤호동(Ho Dong Yoon),이상민(Sang Min Lee),김경길(Kyung Gyl Kim) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.3
Myofibrillar ATPase activity, thermostability and composition of muscle protein were investigated to elucidate biochemical properties regard with rearing period and sex of olive flounder. Myofibrillar ATPase activity of male olive flounder reared for 6, 12 and 20 months was stronger than that of female one. Mg^(2+) (+Ca^(2+))-ATPase activity of both female and male fish decreased with the elapse of rearing period, and the activity of male was higher than that of female far beyond the rearing periods. The high correlationship between the weight gain and myofibrillar ATPase activity was observed. The ther mostability of male myofibrillar protein was higher than that of female. Subunit composition of the myofibrillar and sarcoplasmic protein did not show difference between the both sex of the fish.
넙치 , 범가자미 및 잡종 ( 넙치 ♀ × 범가자미 ♂ ) 근육의 식품화학적 특성 비교
김태진(Tae Jin Kim),민진기(Jin Gi Min),윤호동(Ho Dong Yoon),이두석(Doo Seog Lee),박정흠(Jeung Hum Park),손광태(Kwang Tae Son),김경길(Kyung Gyl Kim) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.1
The proximate composition, mineral content, fatty acid composition, ATP related compounds, amino acid compositon, color and texture were investigated with dorsal and ventral muscle from olive tlounder, spotted flounder and hybrid (olive flounder ♀ × spotted flounder ♂). Spotted flounder and hybrid were higher in moisture content, and lower in crude protein content than those of olive flounder. Patassium content in hybrid was higher than that in olive flounder and spotted flounder. Hybrid was lower in calcium, iron, manganese content, and higher in magnesium content than olive flounder and spotted flounder. The contents of saturated fatty acid, unsaturated fatty acid and docosahexaenoic acid (DHA) in hybrid were intermediate level of spotted flounder and olive flounder. Fatty acid composition of dorsal muscle was slightly similar to ventral muscle. Adenosine triphosphate (ATP) and its related compounds contents and amino acid content in hybrid were intermediate level of spotted flounder and olive flounder, and these compounds of dorsal muscle were slightly similar to those of counterpart. The major amino acids such as aspartic acid, glutamic acid, leucine and lysine were very similar to all the samples. Total amino acid and essential amino acid contents in dorsal muscle were slightly higher than those in ventral muscle. Free amino acid content and composition in hybrid were similar to spotted flounder, and free amino acid content in dorsal muscle was higher than in ventral muscle. The lightness of hybrid and spotted flounder was stronger than that of olive flounder, and was stronger in dorsal muscle. The breaking strength of hybrid was slightly lower than that of spotted flounder, and was stronger in dorsal muscle.