제주지역에서 분리한 감자 줄기검은병균의 유전적 특성

서상태,이승돈,장한익,임춘근,이중섭,한경숙 한국식물병리학회 2005 식물병연구 Vol.11 No.2

A collection of 12 Erwinia carotovora strains from blackleg diseased potato in Jeju island was characterized genetic diversity by E. carotovora subsp. atroseptica (Eca)-specific PCR, PCR-RFLP of the two genes (16SrRNA and pel) and repetitive sequence PCR (ERIC-PCR). The results were compared with those of the other E. carotovora representative strains. None of the blackleg strains produced PCR amplicons with Eca-specific primers in contrast to the single 690 bp amplicon obtained with Eca strains. In addition, on the basis of pel gene RFLP with Sau3AI, the blackleg strains belonged to the pattern 2 whereas Eca strains belonged to the other one (pattern 3). By analysis of 16S rDNA RFLP generated with Hinf I, the most strains including the E. carotovora subsp. carotovora (Ecc) representative strains used in this study belonged to the pattern 1 whereas the blackleg strains belonged to the pattern 2 except for one strain. Moreover, ERIC-PCR analysis showed that the blackleg strains were closely related to each other and had an unique DNA band. Based on these molecular approaches, we have confirmed that the blackleg disease of potato is caused by a different E. carotovora from Eca and Ecc in Jeju island. 제주지역의 감자 줄기검은병징으로부터 분리한 12균주의 유전적 특성을 분석하기 위해 Eca-specific PCR, PCRFig. RFLP, ERIC-PCR을 실시하여 그 결과를 E. carotovora 대 조균들과 비교하였다. Eca-specific PCR 결과 Eca 대조균들은 특이적 밴드를 형성한 반면, 줄기검은병균은 특이적 밴드를 형성하지 않았다. 또한, pel 유전자의 RFLP 분석 결과 줄기검은병균은 pattern 2를 나타내었으나, Eca 균주 는 pattern 3을 나타내어 Eca와는 다른 특성을 보여주었다. 16S rDNA의 RFLP 분석결과 이번 실험에 이용된 대부분의 균주가 pattern 1을 나타냈지만, 12개의 줄기검은 병균 중 11개의 균이 pattern 2을 나타내어 Ecc와도 다른 특성을 보여주었다. 제주지역의 무름병징과 줄기검은병징을 나타내는 균주들의 유전적 관계를 분석하기 위해 ERICPCR을 실시한 결과 줄기검은병균들은 특이적 밴드를 형성하였으며, 서로 높은 유연관계를 보여주었다. 따라서 제주지역의 줄기검은병징으로부터 분리한 균주들은 Eca, Ecc 균들과는 다른 특성을 가지고 있음을 알 수 있었다.

벚나무 빗자루병균 Taphrina wiesneri의 유전적 특성

서상태,정수지,이승규,김경희 한국식물병리학회 2011 식물병연구 Vol.17 No.1

The ascomycetous fungus Taphrina wiesneri, the pathogen of cherry witches' broom, is highly pathogenic to Prunus yedoensis, the most widely planted cherry trees in Korea as park and roadside trees. A collection of 13strains of the pathogen in Korea and Japan was characterized by 18S rDNA gene sequence and restriction fragment length polymorphism (RFLP) analysis. In cluster analysis based on 18S rDNA gene sequence the strains were divided into 2 clusters. In RFLP analysis of the rDNA-IGS region using HhaI, the strains were separated into four patterns, B, C, D and G, of which pattern G was new. 자낭균인 Taphrina wiesneri는 한국의 공원과 가로수에 주로 식재되는 왕벚나무에 빗자루병을 일으키는 병원균이다. 한국과 일본에서 분리한 13개의 병원균에 대해 18S rDNA 염기서열 분석을 통한 계통학적 분석과 rDNA-IGS 영역에 대한 RFLP 분석을 실시하였다. 18S rDNA 염기서열 분석을 통한 계통도 분석결과 병원균은 2그룹으로 분류되었다. Hha I 제한효소를 이용한 rDNA-IGS 영역에 대한 RFLP 분석결과 B, C, D, G 4개의 패턴으로 나타났으며, 그중 G 패턴은 새로운 패턴이었다.

우리나라에 분포하는 고추와 토마토 풋마름병균(Ralstonia solanacearum) 계통들의 유전적 다양성

서상태,이승돈,정승룡,박종한,한경숙 한국식물병리학회 2007 식물병연구 Vol.13 No.1

A total of 35 strains of Ralstonia solanacearum isolated from wilted pepper and tomato plants in Korea were analyzed for their genetic diversity by bacteriological, pathological and molecular biological approaches. All the strains were identified as R. solanacearum biovar 4 on the basis of physiological and biochemical tests, and species-specific PCR primers. Pathogenicity of the strains was confirmed by inoculating on 4-week-old pepper and tomato seedlings. Using cluster analysis based on repetitive sequence-based polymerase chain reaction (rep-PCR) genomic fingerprints, R. solanacearum strains isolated from pepper and tomato in Korea divided into 6 groups showing a high degree of genetic diversity at 55% similarity level. The genetic diversity of strains was not significantly correlated with their geographic origins and host plants.

Acidovorax avenae subsp. citrulli에 의한 멜론 과실썩음병

서상태,정승룡,박종한,이중섭,한경숙 한국식물병리학회 2006 식물병연구 Vol.12 No.3

In September and October 2005, melons (Cucumis melo L.) from the commercial greenhouses in Naju and Gwangju exhibited severe foliar necrosis and fruit rot. Leaf symptoms initially appeared as V-shaped, necrotic lesions and extending to the midrib. Symptoms on the fruit were occurred randomly as necrotic and sunken spots. Two isolates from diseased leaves and fruits were identified as Acidovorax avenae subsp. citrulli on the basis of bacteriological and genetic characteristics. Pathogenicity of the isolates was confirmed by inoculating on 3-week-old melon and cucumber seedlings. This is the first report of bacterial fruit blotch of melon in Korea.

포도 잿빛곰팡이병의 생물적 방제를 위한 길항세균 선발

서상태,정승룡,박종한,한경숙 한국식물병리학회 2006 식물병연구 Vol.12 No.3

Botrytis cinerea Pers. was found to be highly virulent to the grapevine plant, especially in greenhouse condition. Pseudomonas species play key roles for the biocontrol of many plant diseases especially in soil. Of the 83 isolates of Pseudomonas spp., a bacterial strain P84, isolated from tomato rhizosphere, was shown to suppress a wide range of phytopathogenic fungi in vitro. The isolate was identified as Pseudomonas putida on the basis of its bacteriological and genetic characteristics. The P. putida P84 strain carry the phlD gene for 2,4- diacetylphloroglucinol biosynthesis and may produce the antibiotics as an antagonistic mechanism involved in biocontrol. The antagonistic activity of the bacterium has a promising implication for its use as a biocontrol agent to control grapevine gray mold.

비파나무 및 홍가시나무 점무늬병의 살균제 방제효과

서상태,신창훈,지권혁,박소영 한국식물병리학회 2011 식물병연구 Vol.17 No.3

2000년대 후반부터 고흥지역의 비파나무(Eriobotrya japonica)와 제주지역의 홍가시나무(Photinia glabra)에 Entomosporium mespili에 의한 극심한 점무늬병이 발생하기 시작하였다. 따라서 방제효과가 높은 살균제를 선발하기 위하여 이 연구를 수행하였다. 효과를 검정한 3개의 농약 중 비파나무 점무늬병에는 propiconazole 처리가 방제가 64.1%로 가장 높게 나타났고, 홍가시나무 점무늬병에는 chlorothalonil 처리가 방제가 87.6%로 가장 높게 나타났다. Thiopanate-methyl 처리는 방제가가 비교적 낮게 나타났고, propiconazole은 홍가시나무에 약해를 나타내었다. Since the late 2000s, a serious Entomosporium leaf spot disease, caused by Entomosporium mespili, has been found frequently on leaves of Eriobotrya japonica and Photinia glabra at a nursery station in Goheung and Jeju, respectively. Studies were conducted to select fungicides that would effectively control Entomosporium leaf spot. Among the three fungicides tested, weekly foliar applications of propiconazole and chlorothalonil effectively reduced disease severity on E. japonica and P. glabra showing control value of 64.1% and 87.6%,respectively. Weekly treatments of thiopanate methyl were less effective. Propiconazole controlled the disease,but, it was phytotoxic to P. glabra.

벚나무 빗자루병균(Taphrina wiesneri)에 대한 살균제의 방제효과

서상태,김경희,이상현,김영말,신상철,신창훈,박종한 한국식물병리학회 2009 식물병연구 Vol.15 No.1

In April 2008, cherry (Prunus yedoensis Matsumura) in Jeju exhibited severe disease of branches forming numerous twigs. Five isolates from diseased leaves were identified as Taphrina wiesneri on the basis of biological and genetic characteristics. Twenty one commercial fungicides were tested for control of the disease in vitro. Of the 21 fungicides, triazole compounds (difenoconazole, propiconazole and tebuconazole) showed relatively good antimicrobial activities. Results from the planta bioassays indicated that triazole compounds reduced the witches' broom disease showing control value 25.7~52.8% compared with the nontreated controls. In April 2008, cherry (Prunus yedoensis Matsumura) in Jeju exhibited severe disease of branches forming numerous twigs. Five isolates from diseased leaves were identified as Taphrina wiesneri on the basis of biological and genetic characteristics. Twenty one commercial fungicides were tested for control of the disease in vitro. Of the 21 fungicides, triazole compounds (difenoconazole, propiconazole and tebuconazole) showed relatively good antimicrobial activities. Results from the planta bioassays indicated that triazole compounds reduced the witches' broom disease showing control value 25.7~52.8% compared with the nontreated controls.

Pseudomonas syringae에 의한 사과나무 가지마름병의 발생

서상태,원선영,박덕환,김영숙,허장현,임춘근 한국식물병리학회 1999 식물병연구 Vol.5 No.1

감마선 조사에 의한 고로쇠 수액의 효율적인 저장방법

서상태,오혜영,강하영,Seo, Sang-Tae,Oh, Hye-Young,Kang, Ha-Young 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.1

Effects of gamma irradiation on microbiological changes of Gorosoe sap were characterized during a post-irradiation storage at $4^{\circ}C$. The aseptically collected sap was irradiated and stored at $4^{\circ}C$ for 0 to 60 days and analysed for standard plate counts and 16S rDNA. There were significant differences in the total number of colony forming units(CFUs) of bacteria between irradiated and non-irradiated control sap. Bacteria of non-irradiated sap were present at levels of $1.5{\times}10^4{\sim}2.3{\times}10^8\;CFU/m{\ell}$, whereas no viable microbial cells were detected in sap after 10 kGy of irradiation during storage. According to the 16S rDNA sequence analysis, bacterial community structures decrease with time and the most abundant strain was Pseudomonas species. Our results suggested that gamma irradiation can be used to enhance the shelf-life of Gorosoe sap.

참나무시들음병 나무주사 방제법

서상태 한국수목보호연구회 2013 수목보호 Vol.18 No.-