P Element in Drosophila melanogaster

서동상 한국생화학분자생물학회 1990 생화학분자생물학회 소식 Vol.10 No.4

In the early 1970s, P elements have been discovered by male recombination in Drosophila melanogaster. P elements have revealed much about the molecular structure, mechanism of exicision and transposition, evolution of transposable elements. By serving as mutagens, transposon tags, and transformation vehicles, they have opened new approaches to many other questions in wider area of Drosophila genetics.

헌혈 세트를 이용한 두피하 혈종의 치료 - 치료 수기 -

서동상,김범태,조성진,신원한,최순관,변박장,Suh, Dong-Sang,Kim, Bum-Tae,Cho, Sung-Jin,Shin, Won-Han,Choi, Soon-Kwan,Byun, Bark-Jang 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.11

Sugaleal hematoma usually develop one to eight days after minor head trauma or clotting disorders in children. The galeal aponeurosa in children is loosely attatched to the pericranium, allowing the collection of large quantity of blood. Most cases of subgaleal hematomas resolve spontaneously, however some cases require surgical intervention, aspiration of subgaleal hematoma often alleviate symptoms briefly and but do not shorten the time to resolution. Reaccumulation, infection following aspiration also had been reported. Here, we report the efficacy of using the blood donating set for the treatment of subgaleal hematoma in our series.

한국 노랑초파리 자연집단내의 I 인자의 활성 및 분포

서동상,성기창,노승배,최은희 한국유전학회 1996 Genes & Genomics Vol.18 No.4

Activity and distribution of I elements were analized in the 167 isofemale lines of Drosophila melanogaster sampled from Yoˇng Joˇng Island population in Korea. All isofemale lines were classified to be I strain by SF sterility test. The average copy number of I elements was estimated to be 25.11 copies per haploid genome by in situ hybridization. After about 30 generations, the average activity of I elements showed the tendency to decrease 0.91 to 0.79 and the cytotype of the isofemale lines converted from I cytotype to R cytotype, while they have been maintained in laboratories. The coefficient of correlation was estimated to be 0.454 between the activity and copy number of I elements.

Restriction endonuclease mapping of mitochondrial DNA in silkworms

서동상,성승현,최강준,문정수 한국유전학회 1995 Genes & Genomics Vol.17 No.4

미토콘드리아 DNA는 핵 DNA처럼 histone과 같은 단백질과 결합되어 있지 않은 형태로 DNA변이 축적률이 핵 DNA보다 적어도 10배 이상 된다는 보고가 있고, 세포질유전으로 모계 유전되는 진화적 특징에 의해 많은 생물 종에서 그 염기순서가 연구되어졌다. 그러나 산업곤충으로 실크를 생산하는 누에에 대한 유전자 분석은 거의 초보단계이고 미토콘드리아 DNA에 대한 연구도 아직 되어있지 못한 상태이다. 본연구에서는 산업적으로 사육되고 있는 누에나방속의 누에나방과 같은 과의 멧누에나방 속의 멧누에나방 그리고 계통적으로는 먼 거리에 있지만 견사를 생산하여 고치를 만드는 산누에나방상과의 참나무산누에나방 속 · 참나무산누에나방의 미토콘드리아 DNA를 추출하고 이의 제한효소지도를 작성하여 각각을 비교, 분석하고자 한다. 견사곤충의 미토콘드리아 DNA를 알칼리 방법으로 추출한 다음 다시 Lambda phage vector를 이용하여 미토콘드리아 DNA전체를 cloning하여 미토콘드리아 DNA를 대량 확보할 수 있게 하였다. 전체 미토콘드리아 DNA를 이용하여 RFLP를 분석하였고 보다 자세한 분석을 위해서 플라스미드에 subcloing하여 보다 세밀한 제한효소지도를 작성하고 견사곤충간의 유연관계를 분석하고자 한다.

천잠 피브로인 유전자의 genomic 구조와 5` 상류영역의 부분 염기서열 분석

서동상,이진성,황재삼,윤은영,구태원,김근영,강석우 한국잠사학회 2000 한국잠사학회 잠사과학 100주년 국제심포지엄 및 추계학술대회 Vol.- No.-

후방 경유법에 의한 경추부 수막종 제거후 발생한 전방 경막외 혈종 - 증례보고 -

서동상,김범태,조성진,장재칠,신원한,최순관,변박장,Suh, Dong-Sang,Kim, Bum-Tae,Cho, Sung-Jin,Chang, Jae-Chil,Shin, Won-Han,Choi, Soon-Kwan,Byun, Bark-Jang 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.2

We report a case of anterior spinal epidural hematoma, after removal of cervical meningioma by posterior approach, which occurred in a 61-year-old man who presented with left hemiparesis and hypalgesia. A cervical mass surgically confirmed as meningioma was removed by posterior approach. 3 hours after operation, the patient revealed quadriparesis with respiratory difficulty. We herewith report a rare case of anterior spinal epidural hematoma which caused a catastrophic aggrevation of postoperative course.

뇌동맥류 파열에 의한 뇌지주막하 출혈후 혈관 조영상 혈관연축과 임상적 혈관연축의 상관관계

서동상,김범태,임수빈,조성진,신원한,최순관,변박장,Suh, Dong-Sang,Kim, Bum-Tae,Im, Soo-Bin,Cho, Sung-Jin,Shin, Won-Han,Choi, Soon-Kwan,Byun, Bark-Jang 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.12

Objective : Delayed ischemic neurologic deficit(DIND) is one of the major complications following aneurysmal subarachnoid hemorrhage(SAH). However, the correlation between angiographic vasospasm(AV) and DIND after SAH is not precisely known. The authors investigated the timing, incidence, characteristics of DIND, and analyzed correlation between AV and DIND. Patients and Methods : A series of 126 patients with SAH and performed cerebral angiography which, confirmed anterior circulation aneurysm, admitted to between January 1996 to December 1998, were studied retrospectively. A comparative analysis between group 1(G1) in which AV patients presented with DIND, and group 2(G2) patients did not DIND, were done. AV was graded according to location, distribution and degree. Location of vasospasm was classified as basal type(BT), distal type(DT). BT was involved horizontally and include the bilateral carotid systems, proximal middle cerebral artery(MCA) and proximal anterior cerebral artery(ACA). DT was involved vertically and include the MCA branches as they become vertically or posteriorly oriented and the ACA distal to the anterior communicating artery. BT and DT all defined ether as localized type(LT) or combined type(CT). Distribution of vasospasm was classified as type I, type II and type III. Type I represents the involvement of bilateral carotid systems and bilateral anterior cerebral artery, type II was designed as one carotid system without involving anterior cerebral artery, and type III when only some portions of the anterior cerebral artery were involved, bilaterally. Degree of vasospasm was classified as mild(less than 25%), moderate(between 25-50%), severe(greater than 50%), and those were determined by comparing the caliber of the artery in vasospasm to that of the nearest area of apparently normal vessel. Results : The incidence of AV & DIND was 57/126(45.2%), 29/126(23.0%), and timing of DIND was 9 days(${\pm}4.1$) after initial hemorrhage. As for the location, BT was seen in 12 cases(40.0%), DT 11 cases(36.7%) and CT 7 cases (23.3%), respectively. Where as G1, BT was seen 5 cases(18.5%), DT 5 cases(18.5%) and CT 17 cases(63.0%), respectively in G2. CT AV was more correlated with DIND than LT AV(p<0.05). For distribution, type I was seen in 16 cases(59.2%), type II 4 cases(14.8%), type III 7 cases(25.9%) in G1 where as type I was seen in 7 cases(23.3%), type II 10 cases(33.3%), type III 13(43.3%) in G2. Type I AV was well correlated with DIND unlike to type II or type III(p<0.05). As for the degree, mild was seen in 4 cases(14.8%), moderate 14 cases(51.9%), severe 9 cases (33.3%) in G1, and mild 16 cases(18.5%), moderate 11 cases(36.7%) and severe 3 cases(10.0%) in G2. Moderate to severe type AV was well correlated with DIND(p<0.05). Conclusion : These results indicate that it may be possible to predict DIND according to careful analysis of location, distribution, degree of AV in patients with aneurysmal SAH.

새로운 플라스미드 DNA, BSjunBrp의 작성

서동상,권무식 成均館大學校 科學技術硏究所 1992 論文集 Vol.42 No.2

BALB/c 3T3세포의 protooncogene JunB cDNA의 Hand Ⅲ-Sma I DNA 절편을 phagemid Bluescript M13의 Hand Ⅲ-Sca I을 제거한 multiple cloning 위치에 subclone하여 재조합 플라스미드 BSjunBrp를 형성하였다. BSjunBrp를 Not I으로 선형화하여 T3 promoter를 이용하여 sense strand RNA (721)를 합성하는 한편, BSjunBrp를 Hand Ⅲ로 선형화하여 T7 promoter를 이용, ^32p로 표품된 antisense RNA(694)를 합성하였다. Sense와 antisense RNA hybridization시키고, RNAse A와 RNAse T1로 처리하면 668bp 크기의 protected fragment를 얻을 것이다. A 643 by fragment of p465.20 plasmid having JunB cDNA defined by a Hind Ⅲ site located between 49 nt upstream of the translational initiation codon and a Sea I site(643 nt from the Hind Ⅲ site) was inserted into the Bluescript KS M13+ vector between the Sal I and Sma I sites of the polylinker to generate BSjunBrp. The BSjunBrp phagemid, thus, contains the first 594 pb of the JunB cDNA. Transcription of the Hind Ⅲ digested plasmid with T7 RNA polymerase in the presence of ^32P-GTP yielded a 694 nt probe. Synthetic standard JunB mRNA was made from the same plasmid by digestion with Not I and transcription with T3 RNA polymerase to yield a 721 nt RNA which protected 608 nt of the probe.

F9 細胞에 있어서 Endo A의 면역학적 동정

서동상,권무식 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.1

Murine embryonal carcinoma cells (F9, 22) were differentiated by the treatment of retinoic acid(5 x10 exp (-6)M/1) for 72 hours. The differentiated cells were fixed with 2% glutaraldehyde plus 2% formaldehyde in PBS for 5 min at 4℃, and were reacted with TROMA I(monoclonal antibody of Endo A), followed by FITC-conjugated sheep anti-mouse antibody for immunofluorescent staining of Endo A. It was observed that not all the differentiated cells were fluoresced, and that the degree of the staining intensity was various among the fluoresced cells. These results could indicate that the expression of Endo A filaments in the differentiated F9 cells by the treatment of retinoic acid, is supposed to be heterogeneous.

Detection of Factor Ⅸ Gene Mutations in Korean Hemophilia B Patients by Polymerase Chain Reaction - Single Strand Conformation Polymorphism(PCR - SSCP)

서동상,김봉윤,조율희 한국유전학회 1995 Genes & Genomics Vol.17 No.4

Hemophila B is an X-linked bleeding disease, resulting from sequence alterations of the coagulant factor IX gene, Hemophilia B is caused by a variety of mutations, which can be found in the whole coding regions. We screened mutations of factor IX gene in 8 Korean hemophilia B patients. Amplifications of eight exons, promoter region, and their intron boundaries, were performed with polymerase chain reactions (PCRs) and the PCR products were analyzed by single strand conformation polymorphism (SSCP) with Phast System^(TM) employing silver staining protocol. PCR-SSCP is a powerful technique that can be used to detect base subsitutions. Now this technique was used expendingly for molecular biology and medical part. Here, we have detected six mutations of an altered migration pattern of single strand DNA. Each patients have different mutations in exon B, G and H. And now, we are identifying six different Hemophilia B mutations by direct sequencing.