Sarcoma 180 유발후(誘發後)의 생쥐의 생존(生存) 시간(時間)에 대(對)한 Cyclophosphamide, Corynebacterium Parvum 및 Tubercin-3의 단독(單獨) 및 병합역여(倂合役與)의 영향(影響)

김희태,김인수,서대규,Kim, Hee-Tai,Kim, In-Soo,Suh, Tae-Kyu 대한약리학회 1981 대한약리학잡지 Vol.17 No.1

Eighty of Sarcoma 180 bearing mice, averaging 30 gm of body weight, were divided into eight groups of animals receiving Saline as the control, Corynebacterium parvum, Tubercin-3 and Cyclophosphamide alone and Cyclophosphamide combined with C. parvum, with Tubercin-3 and with both C. parvum and Tubercin-3 and Tubercin-3 combined with C. parvum respectively. Treatment was initiated 4.8 hours after tumor implantation and repeated three times once a day. Doses were suspended or dissolved in 0.2 ml of Saline: 1.4 mg of C. parvum: 0.5 micrograms of Tubercin-3; and 2.7 mg of Cyclophosphamide either in alone or in combination. All the agents given were administered subcutaneously but Cyclophosphamide was given intraperitoneally. The observation on the general conditions of animal took place twice a day following the treatment until the time of death after tumor implantation was determined. Average survival days in each group were as follows: In Control, Saline (11.2 days), C. parvum (14.8 days), Tubercin-3 (16.7 days), Cyclophosphamide(18.7 days). In combination therapy, Cyclophosphamide with C. parvum(22.8 days) with Tubercin-3 (26.9 days). Cyclophosphamide with both C. parvum an Tubercin-3, however, was somewhat longer than in Cyclophosphamide alone but shorter than in combined with either one of C. parvum or Tubercin-3. Finally, in combination with immunotherapeutic agents, Tubercin-3 and C. parvum each other it (8.2 days) was shorter even than Control. Life span of host is, in generally, inversely related to the number of malignant cells and conclusively, the therapeutic potentiation was reflected to be extended survival in combined treatment of a chemotherapeutic Cyclophosphamide with either one of immunotherapeutics, Tubercin-3 or C. parvum. Tubercin-3 and C. parvum in combination, however, appeared to be antagonistic each other.

Biphenyldimethyl dicarboxylate(DDB)가 Ethanol 유발 간독성 흰쥐에서의 지질 과산화와 Oxygen Free Radical 제거 효소 활성도 및 간기능에 미치는 영향

송호연(Ho-Yeon Song),하경란(Kyung-Ran Ha),고현철(Hyun-Chul Koh),신인철(In-Chul Shin),서대규(Tae-Kyu Suh) 대한약리학회 1994 대한약리학잡지 Vol.30 No.2

In an attempt to define the effects of Biphenyldimethyl dicarboxylate(DDB) on the lipid peroxidation, oxygen free radical scavenging enzymes activities and hepatic functions in ethanol-induced hepatotoxic rats, we studies malondialdehyde(MDA) level and the activities of catalse, superoxide dismutase(SOD), glutamic-oxaloacetic transaminase(GOT) and glutamic-pyruvic transaminase(GPT) in liver of the rats at 24, 48 and 72 hr after the injection of ethanol and DDB. Sprague-Dalwey albino rats weighing 250 to 280gm were injected intraperitoneally with ethanol(2.5 gm/kg ) only and ethanol plus DDB(300mg/kg ). The result obtained can be summarized as follows : 1) The group treated with ethanol showed significantly higher MDA level and lower catalase and SOD activities at 24, 48 and 72hr after the injection as compared with that of control group. 2) The group treated with ethanol showed significantly higher GOT and GPT activities at 24, 48 and 72hr after the injection as compared with that of control group. 3) The group treated with ethanol plus DDB showed significantly lower MDA level and higher catalase and SOD activities at 24, 48 and 72 hr after the injection as compared with that of ethanol group. 4) The group treated with ethanol plus DDB showed significantly lower GOT and GPT activities at 24, 48 and 72 hr after the injection as compared with that of ethanol group. These results suggest that the excessive oxygen free radicals resulting from the depression of the activities of catalase and superoxide dismutase is an important determinant in pathogenesis of ethanol-induced hepatotoxicity and DDB has antioxidant effects.

慢性腎不全症 患者의 血淸 中 各種 脂質分劃 및 Cholesteryl Ester의 脂肪酸 組成의 定量分析

權會泰,朴鍾茂,徐大圭 한양대학교 의과대학 1983 한양의대 학술지 Vol.3 No.2

Chronic renal failure patients undergoing long-term hemodialysis had been found to have disturbances in lipid transport and poor prognosis because of increasing risk of cardiovascular complications. This study was undertaken to find out whether they might have any significant alterations in HDL and cholesteryl esters similar to those observed in other patients with accelerated forms of atherosclerosis. Serum lipid fractions and fatty acid compositions of cholesteryl esters were determined with high pressure liquid chromatography in 23 patients (15 males, 8 females, mean age 43.1 years) admitted to the hospital for chronic renal failure undergoing hemodialysis and compared with those in 23 normal volunteers of same sex and ages. In patients with chronic renal failure, HDL-cholesterol and cholesteryl linoleate/cholesteryl oleate ratio were significantly decreased by 21.3% (p<0.05) and 20.2% (p<0.001 from those of normal volunteers, respectively. HDL-cholesterol/total cholesterol ratio and cholesteryl linoleate values in patient group were also decreased by 16.8% and 9.9%, respectively, but they were not statistically significant. The results suggest that the lowering of serum HDL-cholesterol concentration and cholesteryl linoleate/cholesteryl oleate ratio might be one of the important risk factors for rapid progression of cardiovascular complications in patients with chronic renal failure undergoing hemodialysis.

新生兒의 血淸 High Density Lipoprotein 分劃中 脂質成分과 Lecithin Cholesterol Acyltransferase의 活性

高昌禹,徐大圭 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.1

Lipid consumption in early life has been implicated with atherosclerosis in later life. It has been also claimed that lipids contents of serum in early life would reflect coronary atherosclerosis in later life. Present study was undertaken to look for the lipid and apoprotein composition of serum high density lipoprotein (HDL) and activities of serum lecithin cholesterol acyltransferase (LCAT) in newborns. In comparison with adults, HDL-phospholipids were significantly increased in newborn. However, HDL-cholesterol/total phospholipids ratios were significantly increased in newborns compared with those of adults. Levesl of KDL₂-cholesterol were significantly decreased in newborns, while levesl of HDL₃-cholesterol showed no significant differences. Serum LCAT activity was also significantly decreased in newborns. HDL-cholestery linoleate/HDL-cholesteryl oleate ratio in newborns were significantly lower than those of adults. Apoprotein A-Ⅰand A-Ⅱ in HDL fractions were also decreased in new-borns.

패랑이꽃(Dianthus Superbus. 瞿麥)의 藥理學的 硏究

徐大圭 慶北大學校 醫科大學 1958 慶北醫大誌 Vol.1 No.1

1. The water-extract of the DIANTHUS SUPERBUS increases the movement of isolated intestine and uterus of the rabbit. The pregnant uterus of rabbit is more susceptible to this extract than the non-pregnant uterus. 2, In living rabbit a marked increase in the uterine movement is observed even when a small amount, which is not sufficient to produce a significant change in the movement of intestine, blood pressure or the rate of respiration, has been administered. This effect is also more marked in the pregnant uterus than the non-pregnant one. 3. The potency of 20mgm of this water-extract of dianthus superbus is equivalent to 0.2mgm of Ergometrine maleate or to 4 units of Posterior Pituitary Injection, U.S.P. 4. This extract possesses the peripheral vaso-constrictor effect. 5. This material inhibits the motility of the cultured unicellular organisms.

흰쥐에서의 Cyclosporine-유발 신독성에 대한 Thromboxane A₂-수용체길항제, KT2-962, 의 효과

김규성,강주섭,서대규 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.2

CsA is presently widely used for as a clincally proved immunosuppressive agents. Unfortunately, however, the therapeutic potential of this drug is often limited by accompanying nephrotoxicity. Various factors and effects have been hypothesized to explain the adverse effect of CsA on the renal function. However, the relative contrivutions of direct or indirect toxicity mediated through renal pathophysiology was related in part to renal vasoconctriction is multifactorial and unclear, but it has been suggested that TXA₂would be one of the important potential mediators of altered renal hemodynamics. In this study, the efficacy of KT2-962(KT2). an azulene derivative of recently synthesized selective TXA₂receptor antagonist, in preventing acute nephrotoxicity in CsA-treated rats was examined and the elevations in urinaty N-acetly-β-D-glucosaminidase(NAG) activity along with BUN, serum creatinine levels, and creatinine clearance. Body weigh and urine outflow were also measured during experiment. Histological changes of the kidney was evaluated by electron microscopy. Male Wistar rats were administered CsA(25mg/kg, i.p) and/or KT2(30mg/kg, p.o) once daily for 20 consecutive days. The pretreament of extra KT2 for 3 days was given in coadministration of CsA+KT2. The results (values are means±SE) obtained can be summarized as follows: 1) Urinary NAG Activity (U/mg of urine creatinine) : It progressively increased to the maximal level, 153.7±11.7 on 14th day and then gradually decreased in the CsA group. KT2 coadministered with CsA, however, almost completely antagonized and normalized CsA-induced NAG activity elevations. 2) BUN Concentration (mg/dl): Concentrations on 3rd weeks of treatment significantly increased in the CsA group (2.9 vs 28.9), but increment significantly inhibited to about 33% of values 3) Serum Creatinine Concentration (mg/dl): It increased to 0.41±0.05 (0.37 vs 0.78) and 0.48±0.05(0.39 vs 0.86) in the KT2 and CsA group respectively on 3rd weeks of treatment. Serum creatinine concentration in the KT2+CsA group were increased 0.39±0.05(0.40 vs 0.79) not significantly different from the CsA group 4) Creatinine Clearance (ml/min) : It increased about 2.2 folds of pretreatment levels (0.42 vs 0.94) on 3rd weeks of treatment in the KT2 group, but decreased to 86% of control levels (0.43 vs 0.37) in the CsA group. In the KT2+CsA group, it significantly antagonized a decrease of creatinine clearance in the CsA group and increased about 2.4 folds of pretreatment levels(0.43 vs 1.02). 5) Urine Output (ml/20hrs) : It slightly decreased in both KT2 and CsA groups, but a decrease of urine output was inhibited and increased significantly to about 2.3 folds of pretreatment value (7.8 vs 17.8) on 6th days of treatment in the KT2+CsA group. 6) Weigh Gain (gm): Body weigh was progressively increased and gained about 36.0±2.3(254.0 vs 290.0) and 28.1±1.4 (280.4 vs 252.3) on 3rd weeks of treatment in the saline and KT2 group respectively. In contrast, there was 28.0±3.3 weight loss (253.1 vs 225.1) in the CsA group. KT2 coadministration with CsA ingibited weight loss with CsA alone and gained 23.5±1.0 (250.9 vs 274.4) in the KT2 CsA group. 7) In the electron microscopic finding of glomerulus ofthe CsA group, thickened basal lamina of capillary, irregular shaped pedicels of podocytes and indistinct slit pores are seen. Bowman's space are not seen. In epithelial cells of proximal convoluted tubule, large size oval vacuoles with dense debris and numerous phagocytes are distributed at apical portion of epihelial cell and in the middle portion of cell, nuclesus is deformed to irregular angular shape. The microvilli and mitochondria are industinct in shapes. But, In order groups, glomerulus and tubular epithelial cell are relatively preserved in normal apperances. Consequently, it is suggested that endogenous TXA₂may paly an important role in CsA-induced nephrotoxicity and that coaministration of selective TXA₂receptor antagonist, KT2-962 can suppress CsA-nephrotoxicity in rats.

Primrose系 種子油의 有效成分 分析

申仁澈,金圭讚,徐大圭 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

Many medicinal products have been obtained in natural source. Recently, Primrose seed oil is suggested to the richest natural source of valuable biologically active polyunsaturates, especially gamma-linolenic acid (GLA), which is concerned to the treatment of various uncontrolled disease related to the disorders of fatty acid metabolism. Thus this study was investigated firstly to confirm the presence of GLA and its content in Primrose seed oil in Korea, and the following results were obtained. 1. 0.16 gm of seed oil were extracted from 1.0 gm of its seed. 2. In the compositions of seed oil tested cis-linoleic acid (66.0%) and GLA (12.3%) were main components. 3. Minute contents of metals identified in seed oil tested were not exceed its limited value. It can concluded that Primrose seed oil in Korea are abundant in cis-linoleic acid especially GLA in regard to other vegetable oil, thus it is valuable for control of variable disease related to the fatty acids.

Gentamicin이 白鼠 尿細管의 燐酸鹽 分解酵素 活性에 미치는 影響

申仁澈,趙旺基,徐大圭 한양대학교 의과대학 1986 한양의대 학술지 Vol.6 No.2

Gentamicin is a bactericidal aminoglycoside antibiotic and an important agent for the treatment of many serious gram-negative bacillary infections. However, emergence of resistant microorganisms and development of nephrotoxicity with long-term use of the antibiotic has become a serious problem and may limit the future use of this agent. It is generally accepted that gentamicin-induced nephrotoxicity is closely related to the accumulation of the antibiotic in renal tubules. This study was made to determine the changes in the activities of alkaline phosphatase and adenosine triphosphatase (ATPase) histochemically in renal tubules of male ICR mice, weighing 20-25 gm, treated with a bolus intraperitoneal injection of gentamicin, 300 mg per kg body weight at 3, 6 and 12 hours after drug administration in order to elucidate the pathogenesis underlying gentamicin-induced nephrotoxicity. The results were as follws. 1. Phosphatase activities after gentamicin administration were decreased in renal cortex. 2. Alkaline phosphatase activity in renal cortex were slightly decreased (?) at 3 and 12 hours and moderately decreased(+) at 6 hours after administration compared to control group(?). 3. ATPase activity in renal cortex were moderately decreased (+) at 3 hours, severe decreased (±) at 6 hours and slightly decreased (?) at 12 hours after drug administration compared to control group (?). Consequently, it is suggested that gentamicin decreases in activities of alkaline phosphatase and ATPase in the kidney by damages in renal epithelial cells and cytoplasmic organelles and thought to be that it is one of the biochemical events in gentamicin-induced nephrotoxicity.

Alcohol의 임상약리학적 고찰

서대규,신인철 漢陽大學校環境科學硏究所 1987 環境科學論文集 Vol.8 No.-

Alcohol이 사람에 미치는 약리작용을 비교 분석하고자 한양대학교 의과대학 학생 174명(남자 117명, 여자 57명)을 대상으로 설문에 의하여 남녀별로 alcohol에 강한 군을 S군, 약한 군을 W군으로 구분하여, 남자 S군에는 15%, W군에는 10%, 여자 S군에는 10%, W군에는 5% alcohol 200ml를 섭취케 하여 야기되는 자각증상과 타각증상을 음주전 5회와 음주후 8회씩 매 15분 간격으로 비교 검토하여 다음과 같은 결과를 얻었다. 1. 체온상승감 및 현훈은 주치의 좋은 자료가 될 수 있으나, 기타 자각증상은 주취의 지표가 되기 어려운 것으로 생각된다. 2. 용량적으로 보았을 때 남녀간에 alcohol에 대한 강도에는 약 3배의 차이가 있는 것으로 생각된다. 3. 심박수가 증가되었다(11%~15%). 4. 주취의 특유증상으로 생각되는 안면홍조와, 심박수 증가 등의 심순환계 반응 간에는 어느 정도의 상관관계가 있었으나, 체온상승감 및 현훈 등의 주취의 지표와는 반드시 대응되지는 않았다. 5. 수축기 혈압은 60분까지는 증가(7%~8%), 그 이후는 감소(-5%~-2%)하였으며, 확장기 혈압도 30분까지는 증가(3%~4%), 그 이후는 감소(-4%~-2%)하였다. 6. 배뇨량 및 회수는 대조군보다 2~3배 많았다. 7. 암산능력과 Tapping능력은 저하되지 않았다. Alcohol ingestion was growing in any country. Although alcohol beverage was widely ingested by youngsters and women especially, studies in clinical response induced by alcohol intake were barely reported. To evaluate studies in clinical response induced by alcohol intake. 174 of medical students (117 of male, 57 of female) in Hanyang University were divided in to S, strong to alcohol and W, weak to alcohol. The group of male S was administrered by 200kml of 15% alcohol, and the groups of male W and female S were administered by 200ml of 10% alcohol, and the group of female W was administered by 200ml of 5% alcohol. Symptoms and objective signs every 15 minutes 5 times before alcohol ingestion and 8 times after alcohol ingestion were analysed. The following results were obtained. 1. Feeling of warmth and dizziness were good index of alcohol inebriation, but other symptoms were not. 2. There was about 3 times differences in alcohol inebriation between male and female because peak time and duration of symptoms in the group of male S administered by 200ml of 15% alcohol and the group of female W administered by 200ml of 5% alcohol were almost similar. 3. Heart rate was increased (11% to 15%). 4. Facial flushing, specific symptom of alcohol inebriation, and cardiovascular response including increase in heart rate were correlated to some extent, but facial flushing and good index of alcohol inebriation, feeling of warmth and dizziness, were not correlated. 5. Systolic blood pressure was increaed in 60 minutes (7% to 8%) and thereafter was decreased(-5% to -2%), and diastolic blood pressure was increased in 30 minutes (3% to 4%) and thereafter was decreased(-4% to -2%). 6. The volume and frequency of urination were greater than control group (twice to three times). 7. The ability of calculation and tapping was not reduced.

合成液體洗劑의 局所塗布가 마우스 皮膚組織에 미치는 影響에 關하여

孫東烈,金仁樹,徐大圭 漢陽大學校環境科學硏究所 1981 環境科學論文集 Vol.2 No.-

國産 合成洗劑의 通常用途인 洗淨, 洗滌時 一次的 接觸이 일어나는 部位인 皮膚組織에 대한 變化를 觀察하기 위해 市中에서 市販되고 있는 合成洗劑中 任意選擇한 5가지 製品의 液體合成洗劑를 50%, 20%,10%,5% 및 1%로 稀釋하여 마우스의 背部 털을 1×2㎝ 로 깍아낸 후 洗劑原液과 이의 稀釋液을 1日 2回 2㎖씩 塗布한 다음 마우스의 一般狀態와 皮膚組織의 肉眼的 및 組織學的 檢査를 實施한 바 通常使用 濃度로 塗布한 境遇에는 마우스 皮膚에 별有意한 變化를 招來하지 않았으나 1%以上의 濃度로 反復 塗布했을 境遇에는 洗劑의 適用濃度와 適用期間에 따라 程度의 差異는 있었으나 대체로 發赤과 浮腫이 생겼고 毛細血管 充血, 角質層의 肥厚, 好中球의 浸潤, 急性 炎症性 變化, 表皮層의 肥厚 및 角化, 脫毛現象을 認定할 수 있었으며 高濃度로 反復 局所塗布했을 境遇에는 自發運動의 減退, 立毛現象, 飼料 攝取量의 減少, 體重 減少等이 일어났다. 5 different liquid synthetic detergents arbitrarily chosen in Korean markets were applied undiluted and in concentration of 50, 20, 10, 5 and 1 per cent directly to the back of mice over a 1×2 cm area two times a day. And then general conditions of mice and histological changes on the skins of mice were examined. The results of the experiment were as follows; 1. Liquid synthetic detergents topically applied in generally used concentration could not cause any significant changes on the skins of mice. 2. Local redness and swelling of the skin were observed with consecutive topical application in high concentration more than 1 per cent. Capillary congestion, thickening of the horny layer, neutrophilic infiltration in the dermis, thickening and hyperkeratosis of epidermis, loss of hair and acute inflammatory changes were histopathologically noted and the depth of these changes were in accordance with the applied concentration and period of detergents. 3. Decreased spontaneous movement, pilomotor stimulation, decreased food intake and decreased body weight were resulted from the local application of the detergents.