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광학분리를 위한 키랄 크라운 에테르를 이용한 키랄공학의 개발과 응용
백만정(Man-Jeong Paik),윤원남(Won Nam Yun),이원재(Wonjae Lee) 한국생물공학회 2012 KSBB Journal Vol.27 No.4
Chiral crown ethers are synthetic macrocyclic polyethers that bind protonated chiral primary amines with high selectivity and affinity. They have been widely used to separate or distinguish the enantiomers of chiral compounds containing a primary amino moiety by high-performance liquid chromatography, capillary electrophoresis, and NMR spectroscopy. In this paper, two important chiral crown ethers including chiral binaphthyl unit and (18-crown-6)-2,3,11,12-tetracarboxylic acid as chiral selectors are focused. And several chiral resolution techniques and their applications in chirotechnology using these chiral crown ethers with related chiral recognition mechanism studies are reviewed. Especially, it was shown that the commercially available HPLC columns based on (18-crown-6)-2,3,11,12-tetracarboxylic acid have been developed and successfully applied for the resolution of various primary amino compounds including amino acids.
고성능 액체크로마토그래피의 다당유도체를 기초로 한 키랄 고정상에서 이동상 첨가제가 키랄 아민의 광학분리에 미치는 영향
백만정(Man-Jeong Paik),윤혜란(Hye-Ran Yoon),이원재(Wonjae Lee) 한국생물공학회 2014 KSBB Journal Vol.29 No.3
Chromatographic enantiomer resolution of chiral amines was performed on several covalently immobilized and coated chiral stationary phases (CSPs) based on polysaccharide derivatives under the mobile phase conditions containing base or acid or acid/base additive. The chromatographic parameters including separation factors and capacity factors were greatly influenced by the nature of the mobile phase containing base or acid or salt additive as well as the used CSPs. When 0.05% triethylamine/0.05% trifluoroacetic acid as an additive in the mobile phase was used on all CSPs in this study, the greatest enantiomer resolution was observed except for Chiralpak AD. Also, it was shown that the change of base additive into acid or salt in the mobile phase may directly affect chiral recognition mechanisms between the chiral selectors and analytes occurring during enantiomer separation, resulting in the change of elution orders.
Measurement of Total Plasma Homocysteine in Patients with Chronic Renal Failure Using HPLC/FLD
Kyung-Ok Lee(이경옥),Bo-Kyung Kang(강보경),Hyung-Jin Roh(노형진),Kwang-Suk Ryoo(류광석),Jeong-Yeon Yoo(유정연),Man-Jeong Paik(백만정),Kang-Hyeob Lee(이강협) 대한의생명과학회 1997 Biomedical Science Letters Vol.3 No.1
본 연구에서는 한국인 만성신부전증 환자에서 심혈관계 질환의 조기진단을 위한 생화학적 표지자로서 homocysteine의 임상적 유용성을 검토하기 위하여, 만성신부전증 환자와 건강인에서 HPLC/FLD (high performance liquid chromatography/fluorescence detector)를 이용하여 혈장 homocysteine 농도를 측정하고 그 결과를 비교하였다. 본 실험방법의 회수율은 98.6±5.8%를 나타내었으며, 0.2 n㏖/L보다 낮은 농도까지 측정이 가능하였고, 2~50 n㏖/㎖까지 직선성이 성립하였다 (correlation coefficient =0.9997). 한국인 중 건강한 정상인 (20명)과 만성신부전증 환자 (90명)에서 혈장 homocysteine은 각각 6.81±1.54 n㏖/㎖과 27.28±14.94n ㏖/㎖이었으며, 환자군에서는 정상인군에 비하여 약 4 배 정도 높은 수치를 나타내었다 (p<0.05). 본 실험의 결과로 볼 때 HPLC/FLD를 이용한 homocysteine 측정은 예민도와 재현성이 높아 routine 실험실 방법으로 유용성이 높을 것으로 생각되며, 또한 혈장 homocysteine의 측정은 만성신부전증 환자의 주요한 사망원인이 되는 폐쇄성 동맥질환을 조기에 진단하거나, 혹은 homocysteine 농도를 저하시키는 치료를 실시한 후 치료효과를 판단하기 위한 생화학적 marker로 활용될 수 있을 것으로 기대된다. Cardiovascular disease has been the leading cause of death among patients with chronic renal failure. Many reports have been described that homocysteine is one of the independent risk factor to the occulsive vascular disease. In this study, HPLC/FLD (high performance liquid chromatography-fluorescence detector) technique was used to measure homocysteine level in patients with chronic renal failure and normal control group. The detection limit and recovery of total plasma homocysteine using HPLC/FLD were 98.6±5.8% and 0.2 n㏖/㎖, respectively. The linearity of this method was established in concentration range of 2~50 n㏖/㎖ (correlation coefficient=0.9997). The concentrations of total plasma homocysteine were 6.81 ± 1.54 n㏖/㎖ and 27.28 ± 14.94 n㏖/㎖ in normal control (n=20) and patient group (n=90), respectively (p<0.05). In this study, the HPLC/FLD method showed high sensitivity and reproducibility for a routine clinical laboratory testing. Moreover determination of homocysteine level in plasma might be useful for a biochemical marker for predicting the cardiovascular diseases and for monitoring of therapeutic effect of lowering homocysteine in patients with chronic renal failure.
Measurement of Total Plasma Homocysteine in Patients with chronic Renal Failure Using HPLC/FLD
Lee,Kyung-Ok,Yoo,Jeong-Yeon,Roh,Hyung-Jin,Paik,Man-Jeong,Lee,Kang-Hyeob,Kang,Bo-Kyung,Ryoo,Kwang-Suk THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1997 Journal of biomedical laboratory sciences Vol.3 No.1
본 연구에서는 한국인 만성신부전증 환자에서 심혈관계 질환의 조기진단을 위한 생화학적 표지자로서 homocysteine의 임상적 유용성을 검토하기 위하여, 만성신부전증 환자와 건강인에서 HPLC/FLD (high performance liquid chromatography/fluorescence detector)을 이용하여 혈장 homocysteine 농도를 측정하고 그 결과를 비교하였다. 본 실험방법의 회수율은 98.6±5.8%를 나타내었으며, 0.2 nmol/L보다 낮은 농도까지 측정이 가능하였고, 2∼50 nmol/ml까지 직선성이 성립하였다 (correlation coefficient=0.9997). 한국인 중 건강한 정상인 (20명)과 만성신부전증 환자 (90명)에서 혈장 homocysteine은 각각 6.81±1.54 nmol/ml과 27.28±14.94 nmol/ml이었으며, 환자군에서는 정상인군에 비하여 약 4배 정도 높은 수치를 나타내었다 (p<0.05). 본 실험의 결과로 볼 때 HPLC/FLD를 이용한 homocysteine 측정은 예민도와 재현성이 높아 routine 실험실 방법으로 유용성이 높을 것으로 생각되며, 또한 혈장 homocysteine의 측정은 만성신부전증 환자의 주요한 사망원인이 되는 폐쇄성 동맥질환을 조기에 진단하거나, 혹은 homocysteine농도를 저하시키는 치료를 실시한 후 치료효과를 판단하기 위한 생화학적 marker로 활용될 수 있을 것으로 기대된다. Cardiovascular disease has been the leading cause of death among patients with chronic renal failure. Many reports have been described that homocysteine is one of the independent risk factor to the occulsive vascular disease. In this study, HPLC/FLD (high performance liquid chromatography-fluorescence detector) technique was used to measure homocysteine level in patients with chronic renal failure and normal control group. The detection limit and recovery of total plasma homocysteine using HPLC/FLD were 98.6±5.8% and 0.2 nmol/ml, respectively. The linearity of this method was established in concentration range of 2∼50 nmol/ml, (correlation coefficient=0.9997). The concentrations of total plasma homocysteine were 6.81±1.54 nmol/ml and 27.28±14.94 nmol/ml in normal control (n=20) and patient group (n=90), respectively (p<0.05). In this study, the HPLC/FLD method showed high sensitivity and reproducibility for a routine clinical laboratory testing. Moreover determination of homocysteine level in plasma might be useful for a biochemical marker for predicting the cardiovascular diseases and for monitoring of therapeutic effect of lowering homocysteine in patients with chronic renal failure.