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방형애 ( Hyeong Ae Bang ),이준협 ( Jun Hyup Lee ),임국환 ( Kook Hwan Rhim ) 대한보건협회 2012 대한보건연구 Vol.38 No.2
Objectives: Body mass index (BMI) associated with anthropometric measures among adults in Korea and Japan, whose people have largely similar physical characteristics and lifestyles, were compared to study similarities and differences between the two countries, for obesity is related with increased risk of morbidity and mortality. Methods: Representative samples from the National Health and Nutrition Examination Surveys in 2009 in Korea and Japan were used, in which statistically significant data from several thousand men and women in both countries were present. General obesity criteria of BMI≥30㎏/㎡as well as BMI≥25㎏/㎡for Asians, suggested by WHO, were used. Results: Obesity rates using BMI≥25㎏/㎡for determining obesity were significantly higher generally for both men and women in Korea than those in Japan, with high confidence level(p<0.01). However, using BMI≥30㎏/ ㎡for determining obesity resulted in higher obesity rate for Japanese men. Conclusion: In light of Koreans` higher obesity rates compared with the Japanese counterpart, establishment of a system for continual monitoring and public awareness campaign, especially for women, by public health authorities are suggested for effective national health management to reduce obesity.
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한국인의 식품관련 위험인식 및 건강정보 문해력 조사연구
방형애 ( Hyeong Ae Bang ),전상일 ( Sang Il Jun ) 대한보건협회 2012 대한보건연구 Vol.38 No.2
Objectives: The purpose of this study was to investigate the risk perception and the health literacy of the public on the food-related materials and issues. Risk perception of the public was compared with that of experts. Methods: An online survey was conducted for 1,000 respondents from June, 8~15th in 2012. We took quota-sampling method for the representativeness in terms of age, gender and region. Data analyses were carried out using SPSS Windows V.17 programs. Results: Consumer groups were ranked in the first in terms of reliability as a food information provider by 43.6%. Respondents strongly expressed their requests of the government to be more strict in the regulatory roles and supervision of the food industry. The main routes of getting food-related information were internet services for the age group of 20`s and 30`s respectively by 39.3% and 41.6%. 37.6% of the male respondents answered they are actively sharing food information with others, in contrast of 60.0% in the female(p<0.001). The score of risk perception was significantly higher in the public than in the experts (p<0.01). Health literacy on the some food-related terminologies and units were low in the range of 6.3%~32.8%. Conclusion: Consumer groups were ranked in the first in terms of reliability as a food information provider and Respondents request the government`s roles in regulatory and supervision of the food industry. The main routes of getting food-related information were tv and radio, and the degrees of information sharing with others were active in the female respondents. The score of risk perception was higher in the public than in the experts. This perception gap implicated that risk communication was more necessary among various stakeholders.
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류재천,최윤정,김연정,김형태,방형애,송윤선 한국환경독성학회 1999 환경독성보건학회지 Vol.14 No.1
Recently, several new methods for the detection of genetic damages in vitro and in vivo based on molecular biological techniques were introduced according to the rapid progress in toxicology combined with cellular and molecular biology. Among these methods, mouse lymphoma thymidine kanase (tk) gene forward mutation assay, single cell gel electrophoresis (comet assay) and transgenic animal and cell line model as a target gene of lac I (Big Blue) and lac Z (Muta Mouse) gene mutation are newly introduced based on molecular toxicological approaches. The mouse lymphoma tk^(+/-) gene assay (MOLY) using L5178Y tk+i- mouse lymphoma cell line is one of the mammalian forward mutation assays, and has many advantages and more sensitive than hprt assay. The target gene of MOLY is a heterozygous tk^(+/-) gene located in 11 chromosome, so it is able to detect the wide range of genetic changes like point mutation, deletion, rearrangement, and mitotic recombination within tk gene or deletion of entire chromosome 11. The comet assay is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakages in mammalian cells. Also, transgenic animal and cell line models, which have exogenous DNA incorporated into their genome, carry recoverable shuttle vector containing reporter genes to assess endogenous effects or alteration in specific genes related to disease process, are powerful tools to study the mechanism of mutation in vivo and in vitro, respectively. Also in vivo acridine orange supravital staining micronucleus assay by using mouse peripheral reticulocytes was introduced as an alternative of bone marrow micronucleus assay. In this respect, there was an International workshop on genotoxicity procedure (IWGTP) supported by OECD and EMS (Environmental Mutagen Society) at Washington D, C, in March 25-26, 1999. The objective of IWGTP is to harmonize the testing procedures internationally, and to extend to finalization of OECD guideline, and to the agreement of new guidelines under the International Conference of Harmonization (ICH) for these methods mentioned above. Therefore, we introduce and review the principle, detailed procedure, and application of MOLY, comet assay, transgenic mutagenesis assay and supravital staining micronucleus assay.
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Production of nitric oxide from the murine macrophages activated with Corynebacterium cutis lysate
Lee, Won-Chang,Lee, Myeong-Jin,Rhim, Kook-Hwan,Kim, Young-Whan,Bang, Hyeong-Ae 대한보건협회 2003 대한보건연구 Vol.29 No.1
본 연구는 Corynebacterium cutis (Ultracron, Virbac, France) 용해질이 murine의 대식세포 활성과정에서 면역활성능을 가진 nitric oxide (NO))의 생성에 미치는 영향에 관한 실험을 실시하고 그 결과를 다음과 같이 요약한다. 면역자극물질로서 C. cutis 용해물로 자극을 준 RAW 264.7 murine의 대식세포(macrophages: 3×105)로부터 면역촉진제의 하나인 NO의 생산농도에 대한 48시간 지속관찰에서 C. cutis 용해물의 농도가 25㎍/㎖에서부터 통계학적으로 유의한 NO가 생성되고 있었으며, 그 최고농도는 100㎍/㎖까지 관찰되었다 (p<0.01). C. cutis 용해물의 세포독성실험에서 MTT (3- [4,5-dimethylthiazol-2-yl] -2,5- diphenyltetrazoium bromide)를 사용한 48시간 관찰에서 C. cutis 용해물의 농도 50㎍/㎖의 농도수준까지는 세포성장억제가 크게 관찰되지 않고 있었다. 이상의 실험결과로 보아 C. cutis lysate 는 면역증강제로서 유의한 효과를 가지고 있는 것으로 관찰되었다.
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