Glufosinate 저항성 LM콩과 근연종 간의 생장 특성 비교 평가

박해림,육민정,김도순 한국잔디학회 2018 Weed & Turfgrass Science Vol.7 No.4

This study was conducted to estimate potential weediness by quantitatively evaluating the growth characteristics associated weedy risk of the living modified (LM) soybean and its relative soybeans. The LM soybean and cultivated soybean Kwangan-kong showed similar growth characteristics with bigger canopy height, stem diameter and leaf area, while shorter stem length than wild soybean, which has indeterminate growth. In the case of reproductive growth and seed production traits, wild soybean produced significantly greater number of flowers, pods, and seeds, and showed greater pod shattering than LM soybean. When low dose of glufosinate was present as selective pressure, the viability of glufosinate-resistant LM soybean was significantly higher than the relatives due to transgene benefit. Since wild soybean has higher adaptability to the natural environment than cultivated soybean and produces greater number of seeds, this suggests that there is a potential weedy risk of the hybrids between LM soybean and wild soybean. Therefore, long-term studies are required on the subsequent generations of hybrids between LM and relative soybeans for risk management of LM soybean in natural and agricultural environment of Korea. 유전자변형(living modified, LM) 작물의 환경 생태계로의 확산 및 생태계 내 지속성은 LM작물의 위해성 평가 및 안전 관 리에 있어 핵심적인 요소이다. LM콩은 국내에서 재배되고 있지는 않지만 식용 및 사료용으로 가장 많이 수입되고 있는 LM 작물이며, 야생종이 자생하므로 국내 콩 유전자 다양성 보전 및 LMO 위해성 관리를 위해 LM콩의 잡초화 가능성 평가 연구 가 필요하다. 따라서 본 연구는 LM콩과 그 모품종인 광안콩 및 일년생 야생콩인 돌콩의 생장특성을 정량적으로 평가하여 LM콩 및 교잡후대종의 잡초화 가능성을 예측하고자 수행되었다. LM콩의 영양생장특성은 전반적으로 광안콩과 유사하였 으며 돌콩에 비하여 경장은 짧았다. 개체당 화기, 꼬투리 및 종자 수는 야생콩이 LM콩에 비해 월등히 많았고 종자 탈립성 역 시 LM콩에 비해 높았다. 다만 제초제 glufosinate 저항성 LM콩의 도입유전자 효과에 의해 제초제가 선택압력으로서 존재 할 경우 LM콩의 생존 경합력은 광안콩 및 야생콩에 비해 현저히 높을 것으로 판단되었다. 이에 따라 자연환경에 대한 적응 성이 높은 야생콩과 제초제 저항성 LM콩 간 교잡에 의해 생산되는 교잡후대종은 잡초화 가능성 높을 것으로 예상된다. 따 라서 LM콩의 위해성 관리를 위해서는 LM콩은 물론 LM콩과 근연종 간의 교잡후대종을 포함한 장기적인 잡초화 가능성 평 가가 필요하다. 본 연구를 통해 확립된 LM콩과 근연종의 정량적 생장 및 생식특성 평가 결과는 국내 농업환경에서 LM콩의 잡초화 가능성을 평가하기 위한 기초 정보로 활용될 수 있을 것으로 기대된다.

황토의 특성과 한우에 대한 효능

박해림 충남대학교 농과대학 2001 논문집 Vol.- No.7

분갈이

박해림 시와세계 2010 시와세계 Vol.- No.30

나무전봇대의 로망

박해림 고요아침 2010 열린시학 Vol.15 No.1

헤이즐넛과 개암 사이

박해림 고요아침 2015 열린시학 Vol.20 No.1

시각장애인을 위한 길 안내용 스마트 지팡이 콘셉트 개발

박해림,이민선,양호정 서비스사이언스학회 2023 서비스연구 Vol.13 No.1

This study aims to improve the usability of the white cane, which is walking equipment that most local visually impaired people use and carry when going out, and to contribute to the prevention of safety accidents and the walking rights of visually impaired people by providing improvement and resolution measures for the problems identified. Also, this study is a study on the visually impaired, primarily targeting the 1st to 2nd degree visually impaired people, who cannot go out on their own without walking equipment such as a white cane, corresponding to 20% among approximately 250,000 blind and low vision people in the Korean population. In the study process, the concept has been developed from the user's point of view in order that the white cane becomes a real help in the walking step of the visually impaired and the improvement of usability of the white cane, the main walking equipment for the visually impaired, are done by problem identification through the Double Diamond Model of Design Thinking (Empathize→ Define → Ideate →Prototype → Test (verify)). As a result of the investigation in the process of Empathy, a total of five issues was synthesized, including an increase in the proportion of the visually impaired people, an insufficient workforce situation to help all the visually impaired, an improvement and advancement of assistive devices essential for the visually impaired, problems of damage, illegal occupation, demolition, maintenance about braille blocks, making braille block paradigms for the visually impaired and for everyone. In Ideate and Prototype steps, situations derived from brainstorming were grouped and the relationship were made through the KJ method, and specific situations and major causes were organized to establish the direction of the concept. The derived solutions and major functions are defined in four categories, and representative situations requiring solutions and major functions are organized into two user scenarios. Ideas were visualized by arranging the virtual Persona and Customer Journey Map according to the situation and producing a prototype through 3D modeling. Finally, in the evaluation, the final concept derived is a device such a smart cane for guidance for the visually impaired as ① a smart cane emphasizing portability + ② compatibility with other electronic devices + ③ a product with safety and convenience.

LM콩과 야생콩인 돌콩의 교잡후대종 종자의 특성 평가

박해림,육민정,김도순 한국잔디학회 2016 Weed & Turfgrass Science Vol.5 No.4

국내에는 야생콩이 전국적으로 분포하고 있기 때문에LM콩으로부터의 야생콩으로 유전자 이동으로 인한 교잡후대종에 관한 연구는 국내 콩 다양성 보전과 LMO 안전관리를 위해 매우 중요하다. 따라서 본 연구는 LM콩과 일년생 야생콩인 돌콩의 교잡후대종 종자의 형태적 및 발아휴면특성을 평가하여 교잡후대종의 잡초화 가능성을 예측하기 위한 기초자료를 제시하고자 수행되었다. 교잡 1세대의 경우 형태적으로 돌콩과 매우 유사하며, 발아휴면특성또한 모본인 돌콩과 유사하여 휴면성이 매우 클 것으로 예측된다. 교잡 2세대 종자는 형태적 특성과 발아휴면특성이부모종의 중간적인 특성을 지니며 모본인 돌콩에 보다 근접한 것으로 확인되었다. 특히 F2의 휴면율은 65.5%에 달할 정도로 매우 높아 잡초화 가능성을 시사한다. 국내 농업환경에서 교잡후대종이 잡초화 되려면 11월 이후에 탈립된 종자가 토양 중에서 월동하여 종자 활력을 유지하고, 휴면이 타파된 후 발아하여 자연 생태계에서 다른 재배종 및야생종들과 경합하여 생존 및 세대진전을 할 수 있어야한다. 따라서 LM콩 및 야생콩 간 교잡후대종의 명확한 잡초화 가능성은 종자의 월동성, 생육특성 및 종자생산성 평가 등을 추가적으로 수행하여 다각적인 측면에서 면밀히평가되어야 할 것이다. With increasing LM soybean import, the concern about unintentional gene flow from LM soybean to wild soybean and consequential weedy risk has been growing. Therefore, we conducted this study to characterize seed traits including germination of hybrids resulted from gene flow from LM soybean to wild soybean in comparison with their parents, LM soybean and wild soybean. Pollen-donor LM soybean seeds were much greater and heavier (about 15.0 g of 100 seed weight) than F2 hybrid (5.7 g), while pollen-recipient wild soybean and F1 hybrid seeds were smallest and lightest (about 2.5 g). F2 hybrid was brown, intermediate between yellow LM soybean seed and black wild soybean seed. These findings indicate that F1 hybrid seeds show similar characteristics with wild soybean, while F2 hybrid seeds show intermediate color and size between two parents. F2 hybrid seed showed intermediate traits between two parents in germination and dormancy rates, which were 35% and 65%, respectively. LM soybean showed no dormancy, while wild soybean showed greater than 90% dormancy. This finding indicates that F2 hybrid show intermediate characteristics in seed germination with high dormancy trait, suggesting a potential weediness of hybrids resulted from gene flow from LM soybean to wild soybean.

달방

박해림 고요아침 2021 열린시학 Vol.26 No.2

미간眉間 넓은 집

박해림 고요아침 2010 열린시학 Vol.15 No.1

국내의 선행 문헌 분석을 통한 디자인권에 신규성과 창작비용이성 연구

박해림,김성현 한국기초조형학회 2020 기초조형학연구 Vol.21 No.5

To assure justifiability and objectivity on a decision method of the Novelty and the Non creative easiness, the standards that clearly interpret and construe the legal principles and the law should change over time. Though preceding studies must update expression ways and quotations for the Novelty and the Non creative easiness on design right, researchers unreasonably quote the terms and views selected or reworked using their logic. Several preceding studies haven't consistently described The Novelty and the Non creative easiness of the context or their conditions. So the following researchers have confused their studies. Therefore, this study arranges the main characteristics of the Novelty and the Non creative easiness of the Design Protection Acts. And I assorted and extracted 248 preceding studies from a lot of journals, articles, theses, publications, commentaries, and etc. with specialists. According to this process, this study arranged the main characteristic of setting emphasis on logical terms and views, drawing the keyword(term) for the Novelty and the Non creative easiness, and deciding point for judgment of the Novelty and the Non creative easiness from them. From the preceding studies, this study sought out several important problems : structural weakness causes confusing utilization between the Novelty and the Non creative easiness, conclusive status need for concept & expression between them, a standard of judgment changes like an organism on their conditions, ambiguity terms make the logical structure weak, and the lack of consistency on selected terms confuse the contents. Then in view of The Design Right, this study arranged the standard of judgment between the Novelty and the Non creative easiness after checking the standard of plagiarism and legal imitation from data of the preceding studies, design decisions, design examination criterion, etc.