Some Properties of Partially Purified Alkaline Inorganic Pyrophosphatase from Mung-bean (Phaseolus radiatus) Seedling

박우철,남민희,Park, Woo-Churl,Nam, Min-Hee 한국토양비료학회 1982 한국토양비료학회지 Vol.15 No.4

발아초기 녹두의 부위별 alkaline inorganic PPase의 활성변화 및 잎 부위에서 부분 정제하여 얻은 효소를 이용하여 효소적 성질을 조사한 결과를 요약하면 다음과 같다. 1. 잎 부위가 타 부위보다 약 2~4배의 더 높은 활성을 나타냈으며 발아가 진행됨에 따라 잎과 뿌리 그리고 지엽에서는 효소 활성이 초기에 증가하다가 점차 감소하는 경향을 보인 반면, 상배축에서는 초기부터 계속 감소하였으며 하배축에서는 계속 증가하는 경향을 보였다. 2. 정제 과정동안 23.9%의 수율로 86배가 정제되었으며 전기 영동상의 Rm value는 0.35였으며 homogenenity는 아니었고, Km value는 0.89mM로 나타났다. 3. 이 효소는 $Mg^{2+}$에 대해 대단히 Specific하였으며 $Cu^{2+}$와 $Fe^{2+}$도 $Mg^{2+}$에 비해 각각 56%, 55%의 activating effect를 나타냈다. 그러나, $Cu^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$와 $Ni^{2+}$은 이 효소에 대해 저해체로 작용하였다. 4. 이 효소는 pH 8-9와 $50^{\circ}C$에서 최대의 활성을 보였으며 열에 대해서도 상당히 안정하였다. Time course of alkaline inorganic PPase activity with different parts of mung-bean sprout and some properties of partially purified enzyme from mung-bean leaves were investigated. The enzyme activity in leaf, root and cotyledon showed a tendency to increase at an early stage and then decrease gradually as germination continued. However, the crude homogenate of epicotyl showed the continuous decline of the enzyme activity but that of hypocotyl showed the continuous increase. In particular, the enzyme activity of leaf fraction was about 2-4 times as high as those of other fractions. The specific activity of the leaf enzyme was increased 86-fold, with a 23.9% yield, upon purification procedures. The purified enzyme from leaves had the Rm value of 0.35 and was not homogenous when judged by disc gel electrophoresis. Using tetrasodium pyrophosphate as a substrate, the apparent Km value for the partially purified enzyme was 0.89 mM. The enzyme was highly specific for $Mg^{2+}$ $CU^{2+}$ and $Fe^{2+}$ was also showed the activating effect of 56% and 55% with $Mg^{2+}$, respectively. However, $Ca^{2+}$), $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$ and $Ni^{2+}$ acted as inhibitors for the enzyme. The pH optimum for the enzyme shifted from 9.0 to 8.0 as the concentration of $Mg^{2+}$ was increased. The enzyme from mun-bean leaf was the most active at $50^{\circ}C$ and considerably stable on heat.

정례적 결혼검진의 가족센터 적용에 관한 효과성 연구

박우철,Park, Woochul 대한가정학회 2022 Human Ecology Research(HER) Vol.60 No.1

The present study examined the effectiveness of repeated marriage checkups at intervals of six months in a Family Center in Seoul. To achieve this, the pilot study protocol (Park, 2019) was adapted, emphasizing strength-based, counseling-oriented approaches. Two couples and family therapists with master's degrees were trained and their practice was monitored to ensure program fidelity. The methods employed for the assessment of marriage checkup were an online survey, a video recording of couple problem-solving, and a clinical interview. A total of 14 couples participated in the 1st checkup, 10 of whom also completed the 2nd checkup. Couples with varying levels of marital satisfaction participated in the program. Descriptive statistics indicated gradual increases over 8-9 months in diverse indices such as marital satisfaction and relationship strength, while other indices fluctuated slightly but converged to higher levels over time. A non-parametric analysis appropriate for small samples demonstrated statistically significant increases over the 8-9 months in marital satisfaction among couples. The current study evidenced the effectiveness of repeated marriage checkups in the Family Center, which is the primary public institution for family policy and services in Korea. Repeated marriage checkups are therefore a promising way to enhance couples' relationship health and improve the family management system of the Family Center.

NCW대비 군 위성통신 구조 분석

박우철,차재룡,김재현,Park, Woo-Chul,Cha, Jae-Ryong,Kim, Jae-Hyun 한국위성정보통신학회 2009 한국위성정보통신학회논문지 Vol.4 No.1

본 미래 전쟁 양상은 정보화 시대의 도래와 함께 다른 분야와 마찬가지로 전쟁수행 여건에도 많은 변화를 가져왔다. 이에 따라 정보우위와 정보전이 중요시되고 네트워크 중심전(Network Centric Warfare) 등 새로운 전쟁수행 개념이 등장하게 되었다. 군 위성통신체계는 미래 NCW 전장환경에서 전장정보의 실시간 전송이 가능하며, 전장의 광역화, 자동화, 신속화 개념을 지원하고, 광역성, 이동성, 생존성 및 융통성이 보장된 전천후 핵심전략 통신체계이다. 이러한 군 위성통신시스템은 실시간 전장정보전송을 위한 광역 대용량 링크, 생존성, 이동간 통신을 제공하는 체계로 분류된다. 본 논문에서는 이러한 기능을 통합하여 지원하는 미군의 차세대 TSAT체계의 주요 운용개념과 핵심기술을 분석하였다. 분석 결과를 바탕으로 NCW 대비한 차세대 군 위성통신체계 상위 구조는 재밍이나 간섭 등의 채널환경에 최적으로 적응하여 서비스가 가능한 전송율, 항재밍 능력, 망 제어 및 망 관리 능력을 갖추어야 하며 고속 이동 차량, 초소형단말, 초고속 무인기 등의 다양한 플랫폼을 지원해야 하는 등 향상방안을 제안하고, 또한 이러한 체계 구조를 위한 소요기술로 적응형 다중안테나, 레이저 링크, 차세대 항재밍 waveform을 제안한다 As the information age comes out, the aspect of future war brings about the many changes in terms of war-fighting environment. Accordingly, information superiority and intelligence-centric warfare have been important and new war-fighting concept such as NCW(network centric warfare) have been turned up. This paper proposed all-weather core-strategy communications systems guaranteeing not only the real-time transmission of the information collected in a battlefield and expansion, automation, and rapidity of a battlefield but also broadband, mobility, survivability, and flexibility. The proposed military satellite communications system is classified into wideband mass capacity link, survivability, and the system supporting OTM(on the move) communication for the real-time transmission of battlefield information. This paper analyzed the essential operation concepts and core schemes of the U.S. Army's next generation system, TSAT(Transformational Satellite Communication System). Base on the analysis results, this paper proposed that the architecture of next generation military satellite communications systems for NCW have to provide the data rate, anti-jamming capability, network control and management capability which are optimally adaptable for the wireless channel environments such as jamming and interference and to support the variety of platforms like high-speed mobile vehicles, micromini devices, super-high speed unmanned aerial vehicles. Finally, this paper also proposed that next generation military satellite communications systems need the technologies such as the adaptable multi-antenna, laser link, and next-generation anti-jamming waveform.

植物培養細臨-Rhizobium에 依한 窒素固定에 関한 硏究

朴愚喆(W. C. Park),谷田澤道彦(M. H. Yatazawa) 한국토양비료학회 1979 한국토양비료학회지 Vol.12 No.1

부부 결혼검진: 부부의 관계건강을 위한 새로운 접근

박우철 ( Park Woochul ) 한국가족관계학회 2019 한국가족관계학회지 Vol.24 No.2

Objectives: It is well-established in the public health literature that marriage relationship health is a powerful predictor of physical and mental health. Inspired by the annual health checkup model in public health, marriage relationship health checkup was developed by Cordova et al.(2001). The present study applied the marriage checkup at one of the Healthy Family and Multicultural Family Support Centers which are nationwide community-based institutions delivering Korean Healthy Family Policy to the community. Method: Sixteen married couples participated in the marriage checkup. They attended the assessment session where survey, observation of problem-solving discussion, and short interview were conducted. Then, the couples participated in the feedback session two weeks later which provided results of the checkup and some recommendations for the relationship. Results: It was found that the marriage checkup couples differentiated from the couples who sought couple therapy in their level of marital adjustment and other characteristics, such as, mental health. The marriage checkup significantly improved the couples’ understanding of relationship, knowing how to improve relationship, and self-efficacy. Participants reported high level of satisfaction with the marriage checkup at the posttest. Conclusions: This study shows that the marriage checkup is a promising program for attracting new families to the center and improving their relationship health. To fit the context of Korean Healthy Family Policy, a new operation system, ‘Family Relationship Health Management System’ in which the marriage checkup plays a pivotal role of attracting and assessing couples/families is proposed.

Lemna-Blue green algae에 依한 窒素固定에 関한 硏究

朴愚喆(W. C. Park),谷田澤 道彦(M. H. Yatazawa) 한국토양비료학회 1979 한국토양비료학회지 Vol.12 No.1

끼움벽체로 보강된 비내진 상세를 갖는 철근콘크리트 골조의 비선형 해석

박우철 ( Park¸ Woo-cheol ),박완신 ( Park¸ Yoan-sin ),한병찬 ( Han¸ Byung-chan ),윤현도 ( Yun¸ Hyun-do ),정수영 ( Chung¸ Soo-young ) 한국구조물진단유지관리공학회 2002 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.6 No.1

Hysteresis failure models for inelastic behavior of macro-models of infilled lightly reinforced concrete frames have been developed. This models are empirical expressions relating three hysteresis parameters which are the simulating deteriorating behavior of strength ( β ), stiffness ( α ),and bond slip (γ) to materials and geometric properties of RC frames. The incorporation of the hysteresis failure models into computer program IDARC-2D is described. Estimate the values for three parameters α, β,and γ which best fit the benchmark hysteresis loops(Aoyama 1984). The system identification procedure performs a trial and error search for a set of initial values of α, β, and γ such that the cumulative error between the predicted and experimentally observed hysteretic energy is minimized. This models were validated against benchmark results and used in a parametric study to evaluate the influence of certain variables to the behavior of infilled lightly reinforced concrete frames.

재생골재를 사용한 고강도 철근콘크리트 보의 전단성능에 관한 실험적 연구

박우철(Park, Woo Chul),이경희(Lee, Kyoung Hee),박완신(Park, Wan Shin),윤현도(Yun, Hyun Do),정수영(Chung, Soo Young) 한국콘크리트학회 2001 한국콘크리트학회 학술대회 논문집 Vol.2001 No.11

현장타설 끼움벽체로 보강된 비내진 상세를 갖는 철근콘크리트 골조의 비선형 해석

박우철(Park Woo-Cheol),박완신(Park Wan-Shin),한병찬(Han Byung-Chan),윤현도(Yun Hyun-Do),정수영(Chung Soo-Young) 대한건축학회 2003 大韓建築學會論文集 : 構造系 Vol.19 No.2

Hysteresis failure models for inelastic behavior of macro-models of infilled lightly reinforced concrete frames have been developed. This models are empirical expressions relating three hysteresis parameters which are the simulating deteriorating behavior of strength (β), stiffness (α), and bond slip (γ) to materials and geometric properties of LRC frames. The incorporation of the hysteresis failure models into computer program IDARC-2D is described. Estimate the values for three parameters α, β and γ which best fit the benchmark hysteresis loops. The system identification procedure performs a trial and error search for a set of initial values of α, β and γ such that the cumulative error between the predicted and experimentally observed hysteretic energy is minimized. Multi-variable regressions were performed to relate α, β, γ as functions of the specimen's material and geometric properties and reinforcement parameters. The empirical expressions resulting from these regression analyses are the hysteresis failure models. This models were validated against benchmark results and used in a parametric study to evaluate the influence of certain variables to the behavior of reinforced concrete frame with infill crp wall.

높이조절이 가능한 강재 맨홀뚜껑의 개발

박우철(Woo-Cheul Park) 한국산학기술학회 2018 한국산학기술학회논문지 Vol.19 No.6

주철 맨홀뚜껑은 제조과정에서 환경오염을 유발하며, 작업환경은 매우 열악하다. 또한, 맨홀 뚜껑의 높이와 도로표면의 높이가 일치하지 않은 경우에는 많은 불편을 초래하며 안전에도 문제를 야기한다. 본 연구에서는 주철 맨홀뚜껑을 대체할 수 있고, 도로 노면 공사 시 도로 노면 높이와 맨홀 뚜껑의 높이를 동일하게 맞추기 용이한 높이 조절이 가능한 강재맨홀 뚜껑 구조를 제안하였다. 요구되는 품질 성능을 만족시키기 위한 맨홀 뚜껑의 구조에 대한 설계변수를 파악하기 위하여 구조 해석을 수행하였다. 요구되는 내하중 용량을 만족하는 맨홀뚜껑을 제작하기 위하여 ㄷ자 모양의 보강대에 대하여 최적설계를 수행하였다. 높이조절부의 원통과 하부 프레임 원통은 강판을 굽힘 가공하여 원형의 형상으로 만든 후 용접에 의하여 제작하였다. 또한, 보강대도 강판을 굽힘 가공하여 제작하였다. 높이 조절용 홈은 CNC 밀링머신에 의하여 가공하였다. 4차례의 시제품을 제작하여 내하중 실험을 수행하였으며, 시험의 결과를 반영하여 새로이 제작하였다. 내하중 시험에서 용접부의 파손은 발생하지 않았으며, 주로 변형은 높이조절 홈과 가셋트 판이 접촉하는 부위에서 발생하였다. 450kN의 부하 하중에 의해 1~2.7mm의 변형이 발생하지만, 하중을 제거한 후에 거의 잔류변형이 없으며, 맨홀뚜껑의 분해 및 재조립이 가능하기 때문에 내하중 평가도 만족하는 것으로 판정받았다. Cast iron manhole lids cause environmental pollution during the manufacturing process, and the work environment is very poor. In addition, if the height of the manhole cover does not match the height of the road surface, it causes considerable inconvenience and safety problems. This study proposes a height - adjustable steel manhole cover that can replace cast iron manhole covers and easily match the road surface with the upper surface of the manhole cover. Structural analysis was performed to grasp the design variable of the structure of the manhole cover, satisfying the required quality performance. To fabricate a manhole cover that satisfies the required load capacity, the optimal design for the U-shaped reinforcement structure was made. The cylindrical shape of the height adjustment part and the low frame were formed by bending the steel sheet into a circular shape and then welding. Reinforcing bars were also made by bending a steel plate. The height adjustment groove was machined by a CNC milling machine. Four prototypes were fabricated and a load bearing test was carried out, and new manhole cover was made reflecting results of the test. In the load bearing test, there was no breakage of the welded part, and deformation occurred mainly at the contact area between the groove and gusset plate. Deformation of 1 to 2.7mm occurred due to a load of 450kN. On the other hand, after removing the load, there was almost no residual deformation, and the load bearing evaluation was judged to be satisfactory because the manhole cover could be disassembled and reassembled.