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추출온도 및 함수율에 따른 오미자 종자유의 이화학적 특성 및 항산화활성
박예근,박윤제,박소연 한국식품영양학회 2022 韓國食品營養學會誌 Vol.35 No.1
Omija seed oil was extracted from Omija seeds, a by-product of Omija, using an eco-friendly pressed extraction method. Physicochemical properties and antioxidant activities of 12 extracts using different combinations of seed moisture content (5, 7.5, and 10%) and extraction temperature (25, 49, 75, and 100℃) were then investigated. The highest extraction yield was 31.33% at moisture of 5% and temperature of 75℃. The lowest acid value was 4.18±0.25 at moisture of 5% and temperature of 49℃ or moisture of 7.5% and temperature of 25℃. Peroxide value, iodine value, and saponification value were the lowest at 0.64±0.56 meq/kg, 159.38± 6.03, and 57.60±9.40, respectively, at moisture of 5% and temperature of 25°C. The content of total polyphenolics was the highest at 4,413±125 mg TAE/100 g when the moisture content was 10% and the extraction temperature was 25℃. DPPH radical scavenging activities of oil extracts at 20~100 mg/mL were 28.68±7.30~87.65±2.20%. The maximum antioxidant activity and IC50 were 87.65±2.20% and <20 mg/mL, respectively, for extract obtained at moisture of 10% and temperature at 100℃. As a result, the extraction yield, acid value, peroxide value, iodine value, saponification value, and so on were excellent at moisture content of 5% and extraction temperature of 25℃ or 49℃. However, the content of total polyphenolic compounds and antioxidant activity were the highest at moisture of 10% and extraction temperature of 100℃. In conclusion, extracting Omija seed oil from Omija seeds, a by-product of Omija, is effective with a pressed extraction method.
김조훈,박예근,안승원,백정애,박동진 한국환경과학회 2021 한국환경과학회지 Vol.30 No.11
This research was carried out to investigate seed germination and growth of the perennial plant Apocynum lancifolium under different NaCl concentrations, with a view for future cultivation on reclaimed land. Initial characterization revealed that the average length and weight of A. lancifolium seed pods was 133.6 mm and 0.23 g, respectively, and the thousand-grain weight was 0.59 g. Upon examining the effects of light on seed germination, we found germination to be 1.7% higher under light conditions (90%) than under dark conditions (88.3%). In terms of the response to salt stress, we found that 90% of seeds germinated in the 0.00%, 0.25%, and 0.50% salt treatment groups. Although salt treatment up to a concentration of 0.5% was found to have little effect on seed germination, the rate of germination decreased at higher concentrations and was completely inhibited in the 2% treatment. We also established that germination rates were higher in seeds sown in horticultural topsoil than in the coarse sandy soil found in the plant’s natural habitats. Although the growth of A. lancifolium tends to decrease with an increase in salt concentration, we found that the stem thickness, fresh weight, and dry weight of A. lancifolium seedlings subjected to 0.25%–1.0% salt were comparable to those of the control seedling that were not exposed to salt. Furthermore, in contrast to those plants subjected to 2.0% salt, these plants continued to grow and remained viable.
NaCl처리에 따른 한국 자생 개정향풀의 유리아미노산 및 항산화 성분 분석
김조훈 ( Jo-hoon Kim ),이재면 ( Jae-myun Lee ),박예근 ( Ye-gun Park ),장매희 ( Mae-hee Chiang ),백정애 ( Jung-ae Baik ) 강원대학교 농업생명과학연구원 2021 강원 농업생명환경연구 Vol.33 No.3
According to the free amino acid content including proline content after NaCl treatment, 21 free amino acids were analyzed. Glutamine content was the highest after 0.25% and 2.0% NaCl treatment and in the control, while arginine content was the highest after 0.5% and 1.0% NaCl treatment. The total free amino acid in Apocynum lancifolium followed the order 2.0% NaCl treatment > 0.5% NaCl treatment > 0.25% NaCl treatment > 1.0% NaCl treatment > control; in particular, the total free amino acid content after 2.0% NaCl treatment (7428.94 mg/kg) was about 3.4 times higher than that in the control (2218.94 mg/kg). The proline content after 2.0% NaCl treatment was 47.49 mg/kg, while it was the lowest in the control. Antioxidant content, such as flavonoid CE (catechin equivalent) and total polyphenol GAE (gallic acid equivalent), and antioxidant activity AAE (ascorbic acid equivalent) were the highest after 0.25% NaCl treatment (29034.17 mg CE/kg, 24607.68 mg GAE/kg, and 39231.67 mg AAE/kg, respectively).