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Cultural Conditions of Streptomyces californicus KS - 89 for the Production of Bluish Purple Pigment
지영애(Young-Eh Chi),이병호(Byeong-Ho Lee),박우열(Woo-Yeol Park),박법규(Bub-Gyu Park),류병호(Beung-Ho Ryu) 한국식품영양과학회 1990 한국식품영양과학회지 Vol.19 No.3
Streptomyces californicus KS-89 에서 생산되는 청자색 색소를 생산하기 위한 기질별 최적조건을 구하였다. 청자색 색소를 생산할 때 요구되는 기질로서는 탄소원으로 가용성 전분과 glycerol이, 질소원으로 글루타민산소다와 질산나트륨으로서 30℃에서 7일 동안에 생산능이 가장 우수하였다. 이때, 황산철은 필수적인 무기질이었다. 결론적으로 청자색 색소의 생산을 위한 최적 배양조건은 2.0% soluble starch, 1% glycerol, 0.5% sodium glutamate, 0.05% sodium nitrate, 0.001% L-proline, 0.025% K₂HPO₄, 0.005% MgSO₄ㆍ7H₂O, 0.04% FeSO₄ㆍ7H₂O, 0.001% thiamine. HCI과 pH는 7.0이었다. The optimal cultural conditions for production of the bluish purple pigment by the cultivation of Streptomyces californicus KS-89 were determined with various substrates. The carbon and nitrogen sources on the production of pigment indicated that soluble starch and glycerol as carbon sources and sodium glutamate, sodium nitrate as nitrogen sources given a maximum yield of the pigment at 30℃ for 7 days. The addition of ferrous sulfate was essential. The highest production of pigment was observed with cultivation in a medium containing 2.0% soluble starch, 1% glycerol, 0.5% sodium glutamate, 0.05% sodim nitrate, 0.001% L-proline, 0.025% K₂HPO₄, 0.005% MgSO₄ㆍ7H₂O, 0.04% FeSO₄ㆍ7H₂O, 0.001% thiamineㆍHCI and pH 7.0.
Characteristics of Bluish Purple Pigment Produced by Streptomyces californicus KS - 89
류병호(Beung-Ho Ryu),지영애(Young-Eh Chi),이병호(Byeong-Ho Lee),박법규(Bub-Gyu Park),박우열(Woo-Yeol Park) 한국식품영양과학회 1990 한국식품영양과학회지 Vol.19 No.3
천연식용 색소의 개발을 위한 하나의 방안으로 Streptomyces californicus KS - 89에 의하여 생산되는 청자색 색소의 물리 화학적 특성을 조사하였다. 청자색 색소를 실리카겔의 칼럼 크로마토그래피에 의하여 분리하였고, C.I.E. chromatic diagram에 의하면 청자색에 속하며 UV 최대 흡수대는 575㎚이였다. 이 색소의 수용액에서의 색조는 pH 5~8 범위내에서는 안정하였고, 자외선에 의하여 영향을 받지 않으나, 때때로 열에 의하여 색상이 약간 흐려진다. 한편 금속염에는 거의 안정하였고, 특히 이 색소는 돌연변이원성과 항암 효과는 없었으며, 또 항생 물질능도 없었음이 밝혀졌다. Aqueous solution pigment produced by Streptomyces californicus KS-89 showed a vivid bluish purple pigment and purified by silica gel column chromatography. The pigment indicated a deep purple color zone by the C.I.E. chromatic diagram, and showed UV absorption maxima at 575㎚. The color intensity in aqueous solution was fairly stable in the ranges of pH 5~8 and was not affected by UV light, however, sometimes it had faded slightly by the heat. It was possible to prevent significantly by the addition of metal salt. Especially, this pigment has no mutagenicity and antitumor activity and it appears to be devoid of antibiotic activity.
우유에서 분리한 호냉성세균의 Heat-Stable Protease 생산조건
박우열,박법규,류병호,김희숙,노명훈 慶星大學校 1990 論文集 Vol.11 No.2
Pseudomonas fluorescens BH-22, which produced a heat stable protease, was isolated from the city milk. The optimum medium for heat stable protease production from the microorganism was as follows ; glucose 3, casein 5, KH₂PO₄ 0.1, MgSO₄ . 7H₂O 0.1 and CaCO₃ 0.1g100ml. The optimum pH of medium for heat stable protease production was pH 8.0, temperature was 20˚C. Proteolytic culture of Pseudomonas fluorescens BH-22 lost the protease activity ranged from 15% to 18% on heating for 30 min at 55˚C and for 20 min at 60˚C. This study was identified to excrete the protease by psychrotropic bacteria in city milk, and also investigated to the optical conditions of protease production and heat stability of the enzyme. The morphological and physiological characteristics of the strain BH-22 were studied according to the methods of Bergey's mannual, Gradwohl's clinical laboratory method and API 20NE Kit. From these results, strain BH-22 was identified as Pseudomonas fluorescens.
청자색 색소를 분비하는 Streptomyces californicus Ks-89의 분리 및 동정
류병호,지영애,박법규,박우열,김동규 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.5
식품산업에 많이 이용되고 있는 인공색소를 천연색소로 대치할 방안의 하나로 청자색 색소를 분비하는 균주를 분리 동정하였다. 토양에서 분리한 Streptomyces 중 청자색 색소를 분비하는 균주인 strain KS-89를 분리하여 그 형태학적, 생물화학적 특성을 Bergey's manual, Nonbomura's classification, Pridham 및 Lyons classification으로 비교한 결과 수용성 청자색 색소를 분비하는 Streptomyces californicus KS-89로 확인 동정되었다. The objective intended for this study is that of providing a fairly practical guide to the use of natural pigment in the food industry. Streptomyces isolated from soil were carried out test for the excretion of their bluish purple pigment. One strain of Streptomyces, strain KS-89 showed a high production of bluish purple pigment on the glycerol starch-glutamate medium. The morphological and physiological characteristics of the strain KS-89 were studied according to the methods of Bergey's manual, Nonomura's classification, and Pridham and Lyons classification. Based on the results obtained in these experiments, strain KS-89 was identified as Streptomyces californicus.
Zygosaccharomyces rouxii OB-03과 Pediococcus halophilus BH-90 의 첨가에 의한 低食鹽된장의 개발
류병호,채영주,박법규,김동규 慶星大學校 1991 論文集 Vol.12 No.2
For the deveolpment of new technique for low salty soybean paste, this study were carried out to the large scale fermentation of soybean paste after the addition of number of mass cells of Zygosaccharomyces rouxii OB-03 and Pediococcus halophilus BH-90. Inoclum size of Z. rouxii )B-03과 P. halophilus BH-90 was adapted to the rate of 1.8*10??/ml and 1.8*10⁴/ml in large scale fermentation of soybean paste. pH and lactic acid content were not markedly changed and, total and formol nitrogen was eluted high levels in 4% and 8% NaCl concentration during fermentation of soybean paste. Z. rouxii B-03 and P. halophilus BH-90 grew well at 4% NaCl than that of 8% and 12% NaCl concentrations and ethylalcohol also produced highest level at 4% NaCl. Glutamic acid and aspartic acid of free amino acid were eluted prodiminantly at 4% NaCl acid and also lactic were high content then that of others.
Purification of thermostable alkaline protease from Pseudomonas fluorescens BH-22
Cho, Kyung-Ja,Ryu, Beung-Ho,Kim, Hee-Sook,Park, Bub-Gyu 慶星大學校 1990 論文集 Vol.11 No.3
Pseudomonas fluorescens BH-22에서 분리되는 내열성 protese를 황산암모늄의 침전한후 DEAE-cellulose 및 Biogel P-100 column으로 정제하였다. 정제효소는 32.2배로 정제되어 수율은 12.7% 였다. 정제효소의 분자랭은 SDS-phage로서는 45,000-66,000이고 Biogel P-100의 gel filteration으로는 66,000-150,000이였다. 정제 효소의 최적 pH는 9.5이었고, 60℃에서 20min동안 안정하였다. 결론적으로 정제된 Protease는 높은 온도와 알카리영역에서 안정하였다.