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오가피(Acanthopanax sessiliflorus Seeman) 열매로부터 지질 화합물의 분리 및 동정
김수연 ( Su Yeon Kim ),이대영 ( Dae Young Lee ),서경화 ( Kyeong Hwa Seo ),노영덕 ( Young Deok Rho ),김계원 ( Gye Won Kim ),최대성 ( Dae Sung Cheoi ),백남인 ( Nam In Baek ) 한국응용생명화학회(구 한국농화학회) 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.55 No.2
오가피(Acanthopanax sessiliflorus Seeman) 열매 10.0 kg을 실 온에서 70% EtOH 수용액으로 추출하고 이 추출물을 ethyl acetate (EtOAC) 분획, n-butyl alcohol 분획, H2O 분획으로 나 누었다. EtOAc 분획에 대하여 silica gel 및 octadecyl silica gel chromatography column chromatography를 반복 실시하여 4종의 화합물을 분리, 정제하였다. NMR, IR, EI/MS, 및 GC/ MS 등을 통하여 화합물 1(stigmasterol), 화합물 2(linoleic acid), 화합물 3(β-sitosterol), 화합물 4(stigmast-5-en-3β,7β-diol) 의 구조를 결정하였다. Acanthopanax sessiliflorus Seeman fruits (Araliaceae) were extracted at room temperature with 70% aqueous ethanol (EtOH). The concentrated extract was partitioned with ethyl acetate (EtOAc), n-butyl alcohol, and H2O, successively. From the EtOAc fraction, four compounds were isolated through the repeated silica gel and octadecyl silica gel (ODS) column chromatographies. According to the results of physico-chemical and spectroscopic data including NMR, IR, and gas chromatography/ mass spectrometer, the chemical structures of the compounds were determined as stigmasterol (1), linoleic acid (2), β-sitosterol (3), and stigmast-5-en-3β,7β-diol (4).
Lactobacillus brevis LH8이 생산하는 효소에 의한 Ginsenoside Rd의 Compound K로의 전환
전림호(Lin-Hu Quan),양지제(Zhiqi Liang),김호빈(Ho-Bin Kim),김세화(Se-Hwa Kim),김세영(Se-Young Kim),노영덕(Yeong-Deok Noh),양덕춘(Deok-Chun Yang) 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.3
인삼사포닌 ginsenoside는 인삼의 주요한 약리성분으로 인삼을 경구투여 시 major 사포닌의 생체 내에서의 흡수는 매우 낮아 인삼사포닌의 약효를 증대시키기 위해서 기존에 많이 존재하는 major 사포닌을 상대적으로 흡수도 잘 되며 약효도 더 뛰어난 minor 사포닌으로의 전환이 요구된다. 본 연구는 김치에서 분리한 β-glucosidase 활성균주 L.brevis LH8이 분비하는 효소를 이용하여 ginsenoside Rd를 compound K로 전환시켰다. L. brevis LH8의 효소액은 반응온도 30℃에서 효소활성이 가장 좋았고 35℃이상에서는 활성이 급격히 저하되었으며, pH 6.0~12.0 사이에서 효소활성이 가장 좋았고, pH 5.0 이하 및 pH 13.0 이상에서는 활성도가 떨어지는 것을 관찰할 수 있었다. 또한 ginsenoside Rd는 반응 48시간부터 ginsenoside F2로 전환되기 시작하였으며, 반응 72 시간 이후에는 대부분 compound K로 전환되었다. Ginsenosides have been regarded as the principal components responsible for the pharmacological and biological activities of ginseng. Absorption of major ginsenosides from the gastrointestinal tract is extremely low, when ginseng is orally administered. In order to improve absorption and its bioavailability, conversion of major ginsenosides into more active minor ginsenoside is very much required. Here, we isolated lactic acid bacterium (Lactobacillus brevis LH8) having β-glucosidase activity from Kimchi. Bioconversion ginsenoside Rd by this bacterium in different temperatures was investigated. The maximum activities of crude enzymes precipitated by ethanol were shown in 30oC and then gradually decreased. In order to compare the effect of pH, the crude enzymes of L. brevis LH8 were mixed in 20 mM sodium phosphate buffer (pH 3.5 to pH 8.0) and reacted ginsenoside Rd. Ginsenoside Rd was almost hydrolyzed between pH 6.0 and pH 12.0, but not hydrolyzed under pH 5.0 and above pH 13.0. Ginsenoside Rd was hydrolyzed after 48 h incubation, whereas ginsenoside F2 appeared from 48 h to 72 h, and ginsenoside Rd was almost converted into compound K after 72 h.
Kim Bong Lyong(金鳳龍),Rho Yeong Deok(盧泳德),Shin Han Kwon(權臣漢) 한국육종학회 1986 한국육종학회지 Vol.18 No.4
Petiole length of soybean is believed to be closely related to lodging and other agronomic characters, however, there have been few studies on it. Determination of representative petiole length from soybean plants is difficult because of the variation of petiole length at each nodes. This study was to determine the representative petiole length from soybean genotype, and relationships between petiole length and other agronomic characters. The petiole of the lower first node was the shortest and became gradually longer on upper nodes in determinate type. Any petiole of 4th to 15th nodes from top could be used as the representative ones, while the average of three petioles from top third to 5th taken at flowering terminate stage or the measurement from the sixth petiole from the top could be also used as the representative. Petiole length showed highly significant positive correlation with the flowering, maturity, and ripening period, but negative correlations were found with 100 grain weight and pod borer damage. One of the reasons for the lodging of Korean native soybeans seemed to be due to their long petioles, and the importance of petiole length in soybeans was fully recognized.