Glutamine 함유 배양액에 첨가한 에너지원이 마우스의 배 발달에 미치는 영향

김주환,박기상,이택후,전상식,송해범,Kim, Ju-Hwan,Park, Kee-Sang,Lee, Taek-Hoo,Chun, Sang-Sik,Song, Hai-Bum 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.1

Objective: Mammalian embryos undergo changes of energy environment for transfer from oviduct to uterus. Also, the human reproductive organ (oviduct, uterus) contains energy sources of different concentration (oviduct - glucose: 0.5 mM, pyruvate: 0.32 mM, lactate: 10.5 mM; uterus - goucose: 3.15 mM, pyruvate: 0.1mM, lactate: 5.87 mM, respectively). This study was conducted to examine the effect of these energy sources added in DMEM with glutamine on the mouse embryo development. Methods: There was used ICR female mouse. Two cell embryos of mouse are collected by method of 'flushing'. Flushing fluid was used Ham's F-10 added to 20% FBS. The collected 2 cell embryos were cultured in media such as Control (only DMEM), group A and B (DMEM supplemented with 0.5 mM and 3.15 mM glucose), and group C and D (DMEM supplemented with 0.1 mM and 0.32 mM pyruvate), and group E and F (DMEM supplemented with 5.87 mM and 10.5 mM lactate). All experimental media supplemented with 20% hFF, respectively. Pattern of embryo development was observed to interval at 24hr during 96hr. Results : The media with glutamine added glucose (group A: 51.0%; group B: 48.4%) was significantly (p<0.05) higher than other experimental group in development into the morula stage after 24 hr in culture, but not significantly different compared with control and the rate of development into the blastocyst was significantly (p<0.05) low in the both of pyruvate (group C: 7.9% group D: 6.8%) and lactate (group E: 7.1%, group F: 7.1%) treatment group after 48 hr in culture. Development into the blastocyst and hatched balstocyst after 72 hr in culture revealed similarly in control (81.9%) and glucose treatment group (group A: 83.3%, group B: 82.8%). However, development into the hatched and attached blastocyst after 96hr in culture revealed significantly (p<0.05) development in the glucose treatment group (group A: 82.3%, group B: 78.5%) than control (63.2%), and its of pyruvate (group C: 34.1%, group D: 34.1%) and lactate (group E: 25.9%, group F: 33.3%) treatment group were significantly (p<0.05) lower than control similar to previous observations. Conclusion : The glucose added to the DMEM with only glutamine, as energy source, was highly to the rate of development compared with control, but the other energy sources were not, synthetically. Above refer to, the human reproductive organ (oviduct, uterus) contains energy sources of different concentration. Thus, further studies are will examine continuously to effects by interaction of different energy sources in the mouse embryo development, and these results will provide to foundation on the human embryo culture.

이기종 유사홀로그램 시스템 간 콘텐츠 자동 가시화 기법

김주환,조동식,Kim, Ju-Hwan,Jo, DongSik 한국정보통신학회 2020 한국정보통신학회논문지 Vol.24 No.11

최근 실감 콘텐츠 체험 기회를 제공하기 위한 유사홀로그램(hologram-like)시스템은 공연, 전시, 교육 등에 활발히 적용 및 서비스되고 있다. 이러한 유사홀로그램 시스템을 구성하는 방법은 피라미드형, 반투명 대형 단일 스크린 형태 등 설치될 장소, 사용 목적, 체험자 인원, 콘텐츠의 형태 등에 따라 다양한 크기, 투영 면수 등으로 구성할 수 있다. 본 논문에서는 형태가 서로 다른 이기종의 유사홀로그램 시스템에서 구동되는 실감 콘텐츠 제작을 용이하게 하기 위해 하드웨어 구성에 맞추어 즉석에서 자동적으로 가시화할 수 있는 기법을 제시한다. 본 논문의 결과에 따르면 유사홀로그램 시스템의 구성에 따라 콘텐츠를 매뉴얼하게 재가공하는 노력이 필요 없이 콘텐츠 개발자는 유사홀로그램 시스템에 투영되는 콘텐츠만을 개발하면 즉석에서 자동으로 보정된 가시화가 진행됨을 알 수가 있었다. Recently, a hologram-like system to provide a realistic experience has been serviced in performances, exhibitions, education. The constructing method for the hologram-like system can be configured in various forms such as a pyramid-typed, a semi-transparent large screen form. However, in various types of hologram-like systems, it is difficult to provide adjustment by changing and revising the content according to the configured hardware characteristics. In this paper, we propose a novel technique that can automatically visualize virtual contents running on heterogeneous hologram-like systems. To change the content to a given hardware configuration, we receive pre-built simple text-based configuration data, and correcting process was performed. According to the results of this paper, we found automatically and easily corrected visualization with the given configuration of the hologram-like system. Also, the problem of reducing the time by manual control in various types of heterogeneous hologram systems was solved.

노후 상수도관 관리실태와 진단을 통한 개선

김주환,최두용,배철호,Kim, Ju-Hwan,Choe, Du-Yong,Bae, Cheol-Ho 한국수자원학회 2019 물과 미래(한국수자원학회지) Vol.52 No.9

생쥐 난소에서 Preantral Follice의 단순 분리법

김주환,박기상,송해범,전상식,Kim, Ju-Hwan,Park, Kee-Sang,Song, Hai-Bum,Chun, Sang-Sik 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.3

Objective: Our present studies were conducted to examine more effective isolating method of preantral follicles from mouse ovaries. Methods: ICR mice (3-6 weeks old) were sacrificed through cervical dislocation and their ovaries were removed and put into watch glasses containing Hams F-10 supplemented with 10% fetal bovine serum (FBS). Preantral follicles were isolated by three different methods; 1) enzymatical method and 2) mincing method, and 3) scraping method. Enzymatical method was carried out as following. Ovaries were bisected with a pair of fine 30G needles. Bisected ovaries were incubated at $37^{\circ}C$ and 5% $CO_2$ incubator in 2-well dish containing Hams F-10 supplemented with collagenase 600 lU/ml and DNAse 20 lU/ml. After 20 min., follicles were isolated by repeated pipetting. Isolated preantral follicles were collected, and the remnant of tissues was placed in incubator and previous procedure was repeated. Mincing method was carried out with a pair of fine 30G needles attached to 1 ml syringes and minced ovary. Scraping method was carried out with a pair of fine 30G needles and scratched to surface of ovary. The differences between isolating methods were analyzed using Student's t-test and Chi-square. Results were considered statistically significant when ${\rho}$ value was less than 0.05. Results: In handling time, mincing or scraping method ($28{\pm}3.42$ min or $16{\pm}1.58$ min) were significantly (p<0.00001) shorter than enzymatical method ($72{\pm}1.69$ min), and scraping method was significantly (p<0.01) shorter than mincing method. Total number of isolated follicles was significantly (p<0.0001) higher in enzymatical method ($49.8{\pm}3.91$) than in mincing or scraping method ($25.3{\pm}2.33$ or $20.5{\pm}1.75$). Isolated follicles in ${\leq}$90${\mu}m$ were significantly (p<0.005) higher in enzymatical method ($15{\pm}1.71$) than in mincing or scraping method ($7.8{\pm}0.98$ or $8.1{\pm}1.31$). In 91~130 ${\mu}m$, isolated follicles were significantly (p<0.0005) higher in enzymatical method ($33{\pm}3.27$) than in mincing or scraping method ($16.3{\pm}1.82$ or $10.7{\pm}1.38$). In ${\geq}$ 131 ${\mu}m$, isolated follicles were not significantly differences between all groups. In equal sizes, the rate of isolated follicles in ${\leq}$ 90 ${\mu}m$ was highest in scraping method (39.6% vs. enzymatical method: 30.1%, p<0.05; mincing method: 30.9%, p=0.11719, NS). Rate of follicles in $91{\sim}130$ ${\mu}m$ was significantly (p<0.05) lower in scraping method (52.7%) than in enzymatical or mincing method (66.3% or 64.5%). Rate of follicles in ${\geq}$131 ${\mu}m$ was highest in scraping method (8.3% vs. enzymatical or scraping method: 3.6%, p<0.05 or 4.6%, p=0.19053, NS). Conclusions: This study suggests that scraping method is simple and useful for isolation of preantral follicles, because this method reduced handling time and recovered enough follicles. The recovered rate of isolated follicles in diameter of 91 ~ 130 ${\mu}m$ was highest in all methods.

자동변속기 클러치 시스템의 동특성 해석

김주환,김현수,Kim, Ju Hwan,Kim, Hyun-Soo 대한기계학회 1996 大韓機械學會論文集B Vol.20 No.1

In this paper, dynamic characteristics of an AT clutch system were investigated considering the dynamics of check ball and hydraulic control valves. Dynamic model of a pressure control solenoid valve (PCSV) was obtained by Bondgraph and permeance method. Also, the clutch piston and check ball dynamics were modeled by considering the effect of centrifugal force of the oil entrapped in the clutch chamber. In order to validate the dynamic models obtained, plunger displacement of PCSV and pressure response of the clutch supply lines were compared with the available experimental data, which were in good accordance with the numerical results. Using the dynamic model of the clutch system, simulations were performed to investigate the effect of the rotational speed on the response of clutch cylinder pressure, clutch piston and check ball displacement, and oil flow rate into the cylinder and flow rate out of the check valve.

강수계열의 상태분류에 의한 Markov 연쇄 모의발생모형

김주환,박찬영,Kim, Ju-Hwan,Park, Chan-Yeong 한국수자원학회 1996 물과 미래(한국수자원학회지) Vol.29 No.6

개수로내의 검변 및 급변 부정류 해석을 위해서 dynamic wave식을 기본방정식으로 하고 이를 불연속 보간함수와 upstream weighting 을 도입한 Petrov-Galerkin 기법에 의해 해석하는 유한요소모형을 개발하였다. 매트릭스 안정성 해석 결과 Petrov-Galerkin기법은 단파장에서의 선택적 감쇠능력과 위상오차에 있어 우수한 것으로 나타났다. 반면에 Preissmann기법은 단파장에서의 선택적 감쇠능력과 위상오차에 있어 열등한 것으로 나타났고, Bubnov-Galerkin 기법은 비감쇠특성을 나타내고 있어 단파장 영역에서 발산해를 일으키는 주요원인임을 확인할 수 있었다. Petrov-Galerkin 방법은 Courant수의 넓은 범위에서 높은 주파수를 가진 진행파에 대한 선택적인 감쇠와 작은 Courant 수의 범위에서 양호한 위상정도를 가지는 이상적인 조합을 나타내고 있어 점변 및 급변 부정류 해석에 있어 이상적인 기법으로 활용될 수 있을 것으로 판단되었다. A finite element model for simulating gradually and rapidly varied unsteady flow in open channel is developed based on dynamic wave equation using Petrov-Galerkin method. A matrix stability analysis shows the selective damping of short wave lengths and excellent phase accuracies achived by Petrov-Galerkin method. Whereas the Preissmann scheme displays less selective damping and poor phase accuracies, and Bubnov-Galerkin method shows nondissipative characteristics whicn causes a divergence problem in short wave length. The analysis also shows that the Petrov-Galerkin method displays the desirable combination of selective damping of high frequency progressive waves over a wide range of Courant number and good phase accuracy at low Courant number. Therefore, the Petrov-Galerkin can be effectively applied to gradually and rapidly varied unsteady flow.

성견에서 조직유도재생술과 구연산, 테트라싸이크린으로 치근면 처치한 경우 치주조직의 치유에 미치는 영향

김주환,김종여,정진형,Kim, Ju-Hwan,Kim, Jong-Yeo,Chung, Chin-Hyung 대한치주과학회 1994 Journal of Periodontal & Implant Science Vol.24 No.1

The ultimate goal of periodontal therapy is to fully reconstruct the periodontal attachment apparatus. Commonly used techniques for treatment of infrabony defects include a combination of root planing, curettage and root treatment. To prevent the apical migration of epithelial cells, the technique of guided tissue regeneration is used. The aim of this study is to compare the effects of root treatment with Citric acid & Tetracycline and Guided tissue regeneration in dogs. Experimental periodontitis was induced by the ligation of orthodontic elastic threads in the upper right and left premolars 3, 4 of five adult dogs for 6 weeks. 4 types of procedures were performed as follows; 1) Control graup : Mucoperiosteal flap 2) Experinental I : GTR used Gore-tex(R) membrane 3) Experinental II : Root treatment with citric acid (PHl) 4) Experinental III : Root treatment with tetracycline HCl (50mg/ml) There after, dogs were serially sacrificed at the 1, 2, 4, 5, 8 weeks, and the specimens were prepared, and stained with hematoxylin-eosin for the light microscopic evaluation. The results of this study were as follows; 1. Junctional epithelium reached to the notch through the furcation area in control group at 8 weeks. 2. In the aspects of the inflammatory cell infiltration, control group showed severe aggregation than experimental group I, II, III through the experimental period 3. New cementum was observed over the notch from 5 weeks in experimental group II 4. In the aspects of the amount of new bone formation, experimental group was better than control group, but there was not significant differences among the experimental group, I, II, III

지역 공동체 네트워크 시급하다

김주환,Kim, Ju-Hwan 한국도서관협회 1996 圖協會報 Vol.37 No.3

지리교육과의 특색과 발전방향

김주환,Kim, Ju-Hwan 한국대학교육협의회 1992 大學敎育 Vol.58 No.-

단풍마 단백질 추출물의 스트레스로 인한 면역력 저하 개선 효과

김주환,이선미,이동철,Kim, Ju Hwan,Lee, Sun-Mee,Lee, Dong-Cheol 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.2

It is noted that Dioscorea quinqueloba is a medicinal herb that is widely used to treat cardiovascular disease and is assessed as useful to treat other various medical conditions. The immunopotentiating effects of the protein extract (DQP-1) from Dioscorea quinqueloba were thus formally investigated in vivo under incident of cold stress. In this case study, the spleen and thymus weight in mice was shown to have decreased after a measured exposure to cold stress, while the adrenal gland weight in the mice was shown to have increased. The systematic oral administration of DQP-1 significantly recovered the weight loss of the spleen and suppressed the adrenal gland hypertrophy during the association with cold stress. Additionally, the DQP-1 also restored the ascorbic acid level in the adrenal gland reduced after cold stress. The cold stress exposure lowered the percentage of $CD4^+$ and $CD8^+$ cells in the mouse thymus as determined by the flow cytometric analysis, as well as the levels of some serum immunological cytokines(interleukin-2, interleukin-12, and interferon-${\gamma}$) in the studied mice. The resulting identified weakened immunity caused by cold stress was also recovered by a treatment with DQP-1. The DQP-1 significantly suppressed the formation of serum enzymes of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase, which were systematically elevated during the cold stress episode. These results indicate that DQP-1 can improve immunity in mice that are characteristically weakened under stress.