세포융합에 의한 간장 발효균의 육종

김종규,Kim, Jong-Gyu 한국식품연구원 1990 食品技術 Vol.3 No.1

일부 음식점 주방의 부유세균 및 부유진균 조사연구

김종규,박정영,김중순,Kim, Jong-Gyu,Park, Jeong-Yeong,Kim, Joong-Soon 한국환경보건학회 2014 한국환경보건학회지 Vol.40 No.2

Objectives: The purpose of this study was to determine airborne bioaerosols such as bacteria and fungi in the kitchens of restaurants, and to assess the effects of thermal factors on the levels of the bioaerosols. Methods: Air samples were taken from kitchens of nine restaurants. An Anderson type air sampler was used for sampling and measurements. Petri dishes filled with a microbiological culture medium (trypticase soy agar for bacteria and Sabouraud dextrose agar for fungi) were used as the sampling surface. Results: The levels of bacterial aerosol measured were $10-10^3CFU/m^3$ and fungal aerosol $10-10^2CFU/m^3$, respectively The mean values of air temperature and relative humidity in the kitchens were $24.6^{\circ}C$ and 46.4%, respectively. Overall, the levels of bacterial aerosol varied by the restaurant type, and fungal aerosol by the business period (p < 0.05). The main effect of air temperature and interaction effect of air temperature and relative humidity onto the bacterial level were significant (p < 0.05), whereas the effects were not significant onto the fungal level. Conclusions: The results indicate a wide variation in the levels of bioaerosols among different kitchens. The observed differences in bioaerosol levels in the kitchens reflect different periods of use. The interactions of air temperature and relative humidity onto the bacterial level suggest that constant attention should be paid to avoid peaks of contamination during the summer season.

식품산업체가 겪는 위기의 분류와 위기 수준 판단

김종규,김중순,Kim, Jong-Gyu,Kim, Joong-Soon 한국환경보건학회 2015 한국환경보건학회지 Vol.41 No.2

Objectives: Food safety has become one of the major public-concerning issues in Korea. In order to set guidelines to create manuals for the response to a food safety crisis by food industry, this paper classified food safety crises and suggested techniques to determine crisis level. Methods: This study clarified common terminologies and definitions including in food safety crises. It reviewed various food safety crises and described characteristics, types, and states of crises. Results: The results of this study suggested that a food safety crisis implied a situation in which hazards/risk spreading in the food supply chain was widely described, causing strong public concern followed by a socioeconomic impact, and therefore, requiring the implementation of a prompt and full response regarding the situation. In terms of seeking response plans, food safety crises might be classified according to the penalties resulting from violations of laws and regulations, causative substances, stages of the food supply chain, and first contact point for incidents. The crisis level for a food safety crisis could be classified according to its severity parameters. The guideline matrix was divided into four major stages: Blue/guarded, Yellow/elevated, Orange/high, and Red/severe. This study also suggested several methods for determining the crisis level, such as the simple judgement method, scoring methods using a check-list and a weighted check-list. Conclusion: The severity of related parameters might be of great importance in understanding a crisis and determining response options/challenges for crisis levels.

수세미외 섬유의 펄프화에 관한 연구 - 섬유의 구조와 펄프화별 특징 분석

김종규,노재성,이종신 ( Jong Gyu Kim,Jae Seong Rho,Jong Shin Lee ) 한국공업화학회 1997 공업화학 Vol.8 No.6

수세미외 그물 섬유를 크라프트법, 알카리 아황산법, 아황산법,알카리 과산화 수소법과 소오다법으로 펄프화하고, 안트라퀴논의 첨가와 미첨가 조건으로 구분하여 증해하였다. 이들의 고해와 미고해분에 대하여 주사 전자현미경(SEM), 섬유 품질 분석기(FQA), 섬유길이 분류기(Clark 4-Screen Classifier), 화상 분석기(Image Analyzer)를 이용하여 특성과 섬유의 구조를 비교 분석하였다. 그 결과는 다음과 같다. 1) 수세미외 섬유의 증해후 해섬 가능 기준의 Pulping 조건은 KP계(160℃, 2시간), ASP계(155℃, 4시간), PAP계(160℃, 1시간)에서 Kappa값이 각각 12, 25, 10 수준으로 비교적 낮은 Total Alkali(약 20%) 조건에서 적정 증해가 가능했다. 2) 각 펄프화별 펄프의 총 증해수율은 KP 50∼55, ASP계 60∼70, PAP계 45∼50%로 SP계의 수율은 매우 높고, KP나 PAP는 일반 비목재자나 목재와 비슷한 수준을 나타냈다. 3) NaOH의 투입량의 증가는 해섬능을 촉진하고, 섬유길이, Curl, Kink Index 등에서 품질의 형태 변화를 보였다. 4) 수세미외 섬유의 펄프화 공정에서 AQ첨가는 탈리그닌 촉진으로 해섬능이 현격하게 향상되고 섬유의 산화 분해를 방지하며, 고해 속도 상승과 피브릴화를 촉진하였다. 5) ASP계는 KP나 PAP보다 Bulk density가 높고, 섬유간 결합이 치밀하고, 섬유의 세포 손상이 감소되었다. 6) 수세미외 섬유는 “C” Stain에 의한 정색 반응으로 청색 또는 청회색의 맑고 투명한 새포벽을 갖는 정색 특성을 나타냈다. Studies were carried out on the preparation of several kinds of pulps from Sponge gourd fiber by KP, ASP, SP, PAP, AP and addition of AQ pulping process. These unbeaten and beaten pulping fibers were observed their characteristics and fiber structure by SEM, FQA, Image analyzer and Micro projector. The results were summarized as follows ; 1) The cooking condition which is the possible defibrilation of Shives are KP base(160℃, 2hr.), ASP base(155℃, 4hr.), PAP base(160℃, lhr.). From the results, the kappa no. had the range of 12, 25, 10 each other. 2) The pulp yields of sponge gourd fiber obtained the range of KP 50∼55%, ASP & 60∼70% and PAP 45∼50%. SP base have the highest and contents of KP & PAP base are much the same as woods. 3) Increasing amount of NaOH on Pulping was accelerated the defibrilation of Shives and was changed a morphology of pulping fiber quality such as fiber length, curl and kink index. 4) Addition of AQ on pulping process of sponge gourd fiber had a affect to raise the rate of delignification while protecting cellullosic components against degradation, especially defibrilation was very excellent, beated pulp much more easily and increased the fibrilation. 5) ASP system have higher bulk density, fiber bonding and protecting cellullosic components against degradation than KP or PAP. 6) The color reactions of the C stain solution showed blue or blue-gray with clean and transparency thin cell wall.

화학물질관리에 대한 이해 15 - 산업독성연구에서의 동물윤리와 대체시험

김종규,Kim, Jong-Gyu 대한산업보건협회 2012 산업보건 Vol.291 No.-

일반인과 식품취급자의 손 위생관리에 관한 비교

김종규,박정영,김중순,Kim, Jong-Gyu,Park, Jeong-Yeong,Kim, Joong-Soon 한국환경보건학회 2012 한국환경보건학회지 Vol.38 No.3

Objectives: This study was performed to investigate hygienic behavior of food handlers and general population focusing on awareness of hand-washing and the microbial load of their hands. Methods: A questionnaire survey and microbiological analysis were carried out for sixty-four people each. Samples for microbiological analysis were collected through the glove-juice method from the hands, and were analyzed for the presence of aerobic plate counts, total coliform, fecal coliform, Escherichia coli, Staphylococcus aureus, and Salmonella spp. according to the Food Code of Korea. Results: In the survey, significant differences between the food handlers and general population (p < 0.01) were found in hand-washing frequency, duration, use of hand-washing agents, washing parts of hands, hand-drying method, and method of turning off water. In eight different situations among the ten particular situations in their daily life, more food handlers responded to wash their hands than general population (p < 0.05). Bacterial load on hands with general population was consistently higher than with food handlers (p < 0.05), however, percentages of positive hands of S. aureus and Salmonella spp. were not. Conclusions: Poor hand hygiene practices were indicated by the positive results for E. coli, S. aureus, and Salmonella spp. on the hands of some respondents in both groups. This study reveals that there is the need for programs or campaigns to increase hand-washing practices of both groups.

화학물질관리에 대한 이해 21 - 에틸렌글리콜 모노 틸에테르(EGME)의 흡입독성에 관하여

김종규,Kim, Jong-Gyu 대한산업보건협회 2013 산업보건 Vol.297 No.-

남자 대학생의 손 씻기 의식과 실천에 관한 연구

김종규,박정영,김중순,Kim, Jong-Gyu,Park, Jeong-Young,Kim, Joong-Soon 한국환경보건학회 2009 한국환경보건학회지 Vol.35 No.1

Hand-washing has been recognized as a critical factor in controlling infection and cross-contamination. This study was performed to investigate hygienic behavior of male university students focusing on awareness of hand-washing, their hand washing practices, and the difference between their awareness and practices. Both a self-administered questionnaire survey and a separate direct observation in restrooms were carried out at a university campus over a four week period. In the survey, 93.6% of the responded students claimed to wash their hands after using toilet. However, just 16.9% of the observed students actually did so, according to a separate and unnoticed observational study. Among the observed students who washed their hands, 25.0% of them used soap, only 5.0% of them washed four parts of their hands, and 15.0% of them washed their hands for more than 10 seconds. Paper towel was the most common hand drying method in the direct observation and also in the survey. Significant differences between the questionnaire survey and the direct observation (p<0.0l) were found in hand-washing practices, duration of hand-washing, using soap, washing parts of the hand, and hand-drying method after using the toilet. This study indicates that there is a remarkable difference between the male university students' awareness of hand-washing and their hand-washing practices. This study reveals that there is the need for programs or campaigns to increase hand-washing practices of male university students.

화학물질 관리에 대한 이해 4 - 산업보건에서 나노입자 독성에 대해 우려하는 이유

김종규,Kim, Jong-Gyu 대한산업보건협회 2011 산업보건 Vol.279 No.-

휴게음식점 주방의 환경위생상태에 관한 조사연구 - 계절별 변화를 중심으로 -

김종규,박정영,김중순,Kim, Jong-Gyu,Park, Jeong-Yeong,Kim, Joong-Soon 한국환경보건학회 2012 한국환경보건학회지 Vol.38 No.2

Objectives: This study was undertaken to assess the sanitary conditions in the kitchens of food court/cafeterias and determine seasonal variations. Methods: We measured environmental factors (air temperature, relative humidity, illumination intensity, noise level), and dropping airborne microbes (bacteria and fungi) in the kitchens of eight food court/cafeterias in four seasons (January, April, July, and October). Air temperature and relative humidity were measured with in/out thermo-hygrometers at 1.2-1.5 m above floor level. Illuminance measurement was performed through the multiple point method of Korean Standards (KS). Noise level was measured by the standard methods for the examination of environmental pollution (noise and vibration) of Korea. The estimation of dropping airborne bacteria and fungi was performed through use of Koch's method. Results: The highest kitchen air temperature was in July, and the lowest in January. The average temperature surpassed $21^{\circ}C$ throughout the seasons, suggesting a higher temperature than required for the safe handling of food. Humidity in all the kitchens was measured in the range of 50-60%. Half of the kitchens showed illumination intensities below 300 Lux in April. It was found that the sound pressure level of noise in almost all of the kitchens was higher than 85 dB (A). The highest levels of dropping airborne bacteria and fungi were noted in July. The numbers of airborne bacteria were higher than those of fungi. The levels of dropping airborne bacteria and fungi were affected by air temperature, relative humidity, season, and place. Conclusions: This study indicates that the kitchen environments were unqualified to supply safe food. The hygiene level of the kitchens should be improved.