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산란 후기 사료 내 거미(Nephila clavata) 유래 단백질 분해효소제(Arazyme<SUP>Ⓡ</SUP>)의 첨가 급여가 난 생산성에 미치는 영향
김정언(J. U. Kim),김재영(J. Y. Kim),김지숙(J. S. Kim),이보근(B. K. Lee),이소연(S. Y. Lee),이완섭(W. S. Lee),유선종(S. J. You),안병기(B. K. Ahn),김은집(E. J. Kim),박호용(H. Y. Park),손광희(K. H. Son),신동하(D. H. Shin),강창원(C. W. Kang) 한국가금학회 2009 韓國家禽學會誌 Vol.36 No.1
강정진(J. J. Kang),김정언(J. E. Kim),홍석관(S. K. Hong),김종덕(J. D. Kim),허영무(Y. M. Heo),조종두(C. Cho) 한국소성·가공학회 2008 소성가공 : 한국소성가공학회지 Vol.17 No.8
Ultra-thin sheet metal forming techniques are required in precision forming of miniaturized and integrated products. In order to manufacture a good quality and low cost ultra-thin sheet metal products, a highly precise high-speed press is needed. The precision of a press is related with its vibration characteristics during pressing operation. This study evaluated the vibration characteristics of a proposed press design using computer simulation. The analysis compares the static deformation characteristics of the slide and the slide motion for the metal forming of an ultra-thin sheet of thickness less than 0.1mm. Further, in order to minimize the vibrations during high speed pressing operation, revolution balances of the eccentric shaft and the balance weight device is also considered. Finally, modal analysis is used to characterize the natural frequency of vibration of the press.
PCR 기법을 이용한 한국내 젖소의 k- casein 과 β- lactoglobulin 유전자형 분석에 관한 연구
이광전(K . J . Lee),김정언(J . U . Kim),이연근(Y . K . Lee),홍경표(K . P . Hong),김경수(K . S . Kim) 한국축산학회 1995 한국축산학회지 Vol.37 No.4
This study was performed to offer rapid and accurate methods of both sire selection and dairy cattle improvement, according to analyzing loci of κ-casein and β-lactaglobulin in Korean dairy cattle. The blood samples used in this study were taken from the 45 Holstein cattle in the nearby Kyung gi province, where milk was sold to Kon-Kuk milk plant. The results obtained were as follows; 1. This study established the extracting method of high quality DNA from the 45 Holsteins blood samples by using phenol and the analysis of κ-casein and β-lactoglobulin polymotphisms by using PCR. 2. Due to the amplification of κ-casein and β-lactoglobulin loci by using PCR, this study confirmed amplified products of 8746p and 961bp, respectively. 3. Due to the restricted enzyme digestion of Taq I and Hind Ill in amplified products of κ-casein, AA type with Hind III and Taq I showed no change of size in amplified products and BB type with Hind III showed 525bp and 350bp fragments and with Taq I showed 558bp and 317bp fragments, respectively. 4. Due to the restricted enzyme digestion of Hph I in amplified products of β-lactoglobulin, all lanes which were digested by restriction enzyme showed 961bp and 741bp fragments and A.4 type showed 220bp, while AB type showed 220bp and 166bp, and BB type showed 166bp fragments, respectively. 5. The κ-casein genotype of Holstein by restricted enzyme digestion gives frequencies of 75.55%, 17.78%, and 6.67%r for AA, AB, and BB types and for A and B alleles being 0.84 and 0.16, respectively. Restriction enzyme digestion of the β-lactoglobulin genotype of Holstein gives frequencies of 34.38%, 43.74%, and 21.88% for AAA, AB, and BB types and for A and B alleles being 0.56 and 0.44, respectively. 6. The fitness test(x²-test) for genotype frequencies of κ-casein and β-lactoglobulin showed gene equilibrium with AA. AB and BB, and β-lactoglobulin indicated Hardy -Weinberg equilibrium in the ratio of being 1:2:1. 7. The genotyping of κ-casein and β-lactoglobulin loci can be utilized for finding the association between genotypes and production traits in dairy cattle.
가계분석에 의한 k- casein 과 β- lactoglobulin 의 유전자형 분리에 관한 연구
이광전(K . J . Lee),김정언(J . U . Kim),이연근(Y . K . Lee),강민식(M . S . Kang),강석형(S . H . Kang) 한국축산학회 1995 한국축산학회지 Vol.37 No.3
This study was performed to offer basic data of both reducing generation interval and improving selection accuracy of dairy cattle for application of parentage test according to analyzing loci of κ-casein and β-lactoglobulin in related Korean dairy cattle using the PCR. The blood samples used in this study were taken from 24 related dairy cattle in Sin-Jung dairy farm and the frozen semen of two straws used in this study were taken from commercial firms. The results obtained were as follows; 1. This study established the extracting method of high quality DNA from the blood samples of 24 related dairy cattle and from two frozen semen samples by using phenol and determined the analyzing method for the polymorphism of κ-casein and β-lactoglobulin using PCR. 2. According to the restricted enzyme digestion in amplified products of κ-casein and β-lactoglobulin using PCR, κ-casein showed AA and AB types, and β-lactoglobulin showed AA, AB, and BB types, respectively. 3. By restricting enzyme digestion, genotypes of κ-caaein and β-lactoglobulin for dairy cows were always derived from genotypes of one parent or both parents. 4. Genetic panems between κ-casein and β-lactoglobulin markers showed independent gene segregations, therefore, in case of being known sire genotypes, genotypes of daughters would be detemuned by probability. 5. For ttte genetic improvement of dairy cattle, if the semen of sire with κ-casein BB or β-lactoglobulin BB were used by the A.I, genotyping method of marker genes can be useful for early identification and selection of dairy calves.