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복진우,이광열,이승기,김병각 ( Jin Woo Bok,Kwang Youl Lee,Seung Ki Lee,Byong Kak Kim ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.2
To develop new pharmacologically active components of Ganoderma lucidum, we examined various fractions of the methanol extracts of its basidiocarps by MTT assay and brine shrimp test For MTT assay of human hepatoma cell (SK-Hep-1), the neutral fraction (GLE) did not show toxic effects. Compared with microscopical photography of SK-Hep-1 after treatment of each fraction, the methanol extract (GLC), the ethyl acetate exhact (GLC) and GLE did not show spreading cells, and EDT values were 31.7, 26.6 and 22.8 ㎍/㎖, respectively. In brine shrimp test, LD_(50) value of GLE was 922.3 ppm. It indicates that GLE is non-toxic. In consequence, we found that GLE is non-toxic and has inhibitory action against SK-Hep-1.
김선영(Sun Young Kim),진미림(Mi Rim Jin),복진우(Jin Woo Bok),최응칠(Eung Chil Choi),김병각(Byong Kak Kim) 한국생약학회 1994 생약학회지 Vol.24 No.4
The hot water extract of the basidiocarps of Lentinus lepideus was purified and fractionated by DEAE-cellulose ion exchange column chromatography and Sepharose CL-4B gel filtration chromatography. The separated fractions were designated Frs. I, II, III, IV and V. When a dose of 50㎎/㎏/day was injected into ICR mice by i.p., the tumor inhibition ratio 81% of Fr. II against solid sarcoma 180 was the highest among the five fractions. To clarify mechanisms of its antitumor activity, it did not show any direct cytotoxicity against tumor cell when Fr. II was examined by MTT assay. But when examined for immunopotentiation activity, Fr. II increased the amount of the superoxide anion in activated macrophages to 1. 2 times and the number of the plaque forming cells to 2.0 times, respectively. And it restored the depressed response of DTH in tumor-bearing mice to the normal level. Fr. II was a proteinbound polysaccharide which was composed of 93.35% polysaccharide, 1.70% protein and 1.85% hexosamine. It was named lepidan.