To develop new pharmacologically active components of Ganoderma lucidum, we examined various fractions of the methanol extracts of its basidiocarps by MTT assay and brine shrimp test For MTT assay of human hepatoma cell (SK-Hep-1), the neutral fractio...
To develop new pharmacologically active components of Ganoderma lucidum, we examined various fractions of the methanol extracts of its basidiocarps by MTT assay and brine shrimp test For MTT assay of human hepatoma cell (SK-Hep-1), the neutral fraction (GLE) did not show toxic effects. Compared with microscopical photography of SK-Hep-1 after treatment of each fraction, the methanol extract (GLC), the ethyl acetate exhact (GLC) and GLE did not show spreading cells, and EDT values were 31.7, 26.6 and 22.8 ㎍/㎖, respectively. In brine shrimp test, LD_(50) value of GLE was 922.3 ppm. It indicates that GLE is non-toxic. In consequence, we found that GLE is non-toxic and has inhibitory action against SK-Hep-1.