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Comparison of the cytotoxicity of bisphenol A and its analogs on human placental BeWo cells
김미진,Jo Ah-Ra,김지영,An Mi-Jin,Shin Geun-Seup,Lee Hyun-Min,Kim Jinho,박진홍,김철홍,Kim Jung-Woong 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.3
Background Bisphenol A (BPA) and its alternatives (BPF, BPS) are widely used in daily consumer products, which commonly expose humans to these chemicals. Bisphenols (BPs) are endocrine-disrupting chemicals widely present in the environment and can accumulate in the entire food chain by circulating in the ecosystem. In humans, BPs intake leads to accumulation in the human body and causes many chronic diseases, including endocrine disruption, immunotoxicity, and cancer. Objective Although several studies have demonstrated the negative eff ect of BPA and its alternatives, studies have yet to be determined whether bisphenols can aff ect human trophoblast cells. Thus, this study aimed to analyze the toxic eff ect of BPs on the human trophoblast cell line BeWo. Results We investigated the cytotoxic eff ect of BPs on human trophoblast cells. The cytotoxicity was measured by the MTS assay and fl ow cytometric analysis that representing the cell viability, cell proliferation, cell cycle progression and apoptotic cell death. BPs had no eff ect on BeWo cell viability at concentrations below 100 mM, and did not cause signifi cant changes in cell proliferation and apoptosis. However, BPA and BPS altered cell cycle progression and checkpoint regulator expression. Conclusion BPs did not aff ect cell viability and proliferation. However, BPS altered the G 0 /G 1 and G 2 /M phases of the trophoblast BeWo cells by increasing cyclin B1 expression.
김미진,김철홍,An Mi-Jin,Shin Geun-Seup,Lee Hyun-Min,김지영,Hwang Jae Yoon,이주현,Kim Jung-Woong 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.4
Backgrounds Heavy metals are environmental pollutants and their breakdown is regarded as a serious risk to human health. Despite increasing evidence that heavy metals have adverse efects in vivo and in vitro, there is no evidence of the efect of heavy metals during placental formation. Objective We determined the efect of heavy metals on cell viability of BeWo human placental cells using MTS assay and live and dead assay. We also evaluated cell proliferation, cell cycle, and apoptosis by heavy metal treatment using FACS analysis. Results Mercury chloride induces severe cell cycle arrest at the sub-G1 phase by the accumulation of cyclin B. Furthermore, we identifed that mercury chloride induces apoptosis by enhancing the activity of caspase-3. However, we were unable to confrm the deleterious efect of lead in BeWo cells. Conclusion Our results suggested that exposure to heavy metals, specifcally mercury chloride, induced cytotoxic efects in BeWo cells through cell cycle arrest and apoptosis.
천리안위성 기상 탑재체의 가시 채널 관측을 이용한 지표면 반사도 산출 - 배경광학두께 보정의 효과 분석 -
김미진 ( Mi Jin Kim ),김준 ( Jhoon Kim ),윤종민 ( Jong Min Yoon ) 대한원격탐사학회 2014 大韓遠隔探査學會誌 Vol.30 No.5
인공위성의 가시 영역 관측으로부터 에어로솔의 정량적인 정보를 산출하는데 있어, 지표면 반사도의 보정은 매우 중요한 역할을 한다. 이에 본 연구에서는 두 가지 방법을 이용하여 천리안위성의 기상탑재체로부터 관측된 가시채널의 반사도로부터 지표면 반사도를 산출하고, 상호 비교 하여 정확도를 검증하고자 하였다. 첫 번째 방법은 최소 반사도법으로, 동일한 화소에서 일정 기간 동안 관측된 반사도 중 최소값이 에어로솔에 의한 영향 없이 지표반사에 의한 영향만을 포함한다는 가정을 기반으로, 대기산란 효과를 보정하여 지표면 반사도를 산출하는 방법이다. 두 번째 방법은 미리 알고 있는 에어로솔 정보를 고려하여 대기-에어로솔 효과를 보정함으로써 지표면 반사도를 얻는 것으로 본 연구에서 대기 보정법 이라 칭한다. 두 번째 방법을 적용하기 위해서는 정확한 에어로솔 정보가 요구되므로, 에어로솔 광학두께의 오차범위가 0.01 (≥ 440 nm) 이내인 것으로 알려진 AERONET의 산출물을 이용하였다. 본 연구의 주요 목적은 최소 반사도법을 통하여 산출되는 지표면 반사도가 어느 정도의 정확도를 가지는지를 파악하는데 있어, 대기 보정법을 통하여 산출되는 값을 기준 값으로 두고 비교 분석을 수행하였다. 또한, 대기 중 존재하는 배경광학두께가 최소 반사도법의 정확도에 미치는 영향을 분석해보고자 하였다. 서울 지역에서 2012년 봄철 기간(3월 ~ 5월) 동안 AERONET 관측지점에서 산출된 결과를 분석 한 결과, 대기 보정법을 통해 산출된 지표면 반사도의 평균이 0.108로 나타났고, 배경광학두께에 대한 고려 없이 최소 반사도법을 통하여 산출된 지표면 반사도는 그에 비해 약 0.012 높은 값을 보였다. 한 편 배경광학두께를 고려하였을 경우 그 차이는 0.010으로 감소하여, 정확도 향상에 기여하였음을 확인하였다. Accurate correction of surface effect from back scattered solar radiance is one of key issue to retrieve aerosol information from satellite measurements. In this study, two different methods are applied to retrieve surface reflectance by using single visible channel measurement from meteorological imager onboard COMS. The first one is minimum reflectance method, which composes the minimum value among previously measured reflectances at each pixel over a certain search window length. This method assumes that the darkest pixel corresponds to the aerosol-free condition, and deduces surface reflectance by correcting atmospheric scattering from the measured visible reflectance. The second method, named as the “atmospheric correction method” in this study, estimates the result by correcting aerosol and atmospheric scattering with ground-based observation of aerosol optical properties. The purpose of this study is to investigate the retrieval accuracy of the widelyused minimum reflectance method. Also, the retrieval error caused by the loading of background aerosol is mainly estimated. The comparison between surface reflectances retrieved from the two methods shows good agreement with the correlation coefficient of 0.87. However, the results from the minimum reflectance method are slightly overestimated than the values from the atmospheric correction method when surface reflectance is lower than 0.2. The average difference between the two results is 0.012 without the background aerosol correction. By considering the background aerosol effect, however, the difference is reduced to 0.010.
김미진 ( Mi Jin Kim ),서의근 ( Eui Keun Seo ),강은석 ( Eun Seok Kang ),김근모 ( Keun Mo Kim ),오영민 ( Young Min Oh ),조병하 ( Byung Ha Cho ),김형우 ( Hyung Woo Kim ),지명진 ( Myoung Jin Ji ),정지원 ( Ji Won Jeong ),박선미 ( Seon 대한소화기학회 2015 대한소화기학회지 Vol.65 No.4
A pyogenic pancreatic abscess mimicking pancreatic neoplasm in the absence of acute pancreatitis is rare. We report four patients who each presented with a pancreatic mass at the pancreas head or body without acute pancreatitis. The presenting symptoms were abdominal pain, fever, or weight loss. Abdominal CT scans showed low-density round masses at the pancreas head or body with/without lymphadenopathy. In each case, a PET-CT scan showed a mass with a high SUV, indicating possible malignancy. Comorbid diseases were identified in all patients: chronic pancreatitis and thrombus at the portal vein, penetrating duodenal ulcer, distal common bile duct stenosis, and diabetes mellitus. Diagnoses were performed by laparoscopic biopsy in two patients and via EUS fine needle aspiration in one patient. One patient revealed a multifocal microabscess at the pancreatic head caused by a deep-penetrating duodenal ulcer. He was treated with antibiotics and a proton-pump inhibitor. The clinical symptoms and pancreatic images of all the patients were improved using conservative management. Infective causes should be considered for a pancreatic mass mimicking malignancy. (Korean J Gastroenterol 2015;65:252-257)