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- 저자 : 김경남(Kyeongnam Kim) (검색결과 3 건)
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모델식물 애기장대에 대한 훈증제 메틸브로마이드의 약해발생 및 N-acetyl-L-cysteine, L-glutathione, indole-3-acetic acid의 약해억제 효과
김경남,김채은,박정은,유진성,김우성,전황주,김준란,이성은,Kim, Kyeongnam,Kim, Chaeeun,Park, Jungeun,Yoo, Jinsung,Kim, Woosung,Jeon, Hwang-Ju,Kim, Jun-Ran,Lee, Sung-Eun 한국환경생물학회 2021 환경생물 : 환경생물학회지 Vol.39 No.3
본 연구는 식물검역 분야에서 주요하게 사용되고 있는 메틸브로마이드 훈증제로 인해 발생하는 약해를 저감하기 위한 물질을 모델식물인 애기장대를 이용하여 스크리닝하였다. 사전연구를 통하여 메틸브로마이드 훈증제의 식물 독성 메커니즘으로 활성산소발생와 식물 성장 호르몬인 옥신의 식물체 내 분배억제효과가 발생하는 것을 바탕으로 하여, 약해 저감물질후보군으로 활성산소를 제거하는 역할을 하는 ROS scavenger 2종(NAC, GSH)과 옥신을 훈증제 처리 전 애기장대에 처리한 후 약해의 저감 정도를 육안평가와 더불어 관련 유전자의 발현을 확인하였다. 연구 결과 메틸브로마이드에 의해 유도된 약해는 옥신보다는 활성산소를 저감시키는 물질후보군들에서 약해 저감효과가 나타났다. 이 중 GSH을 이용하여 농도구배하여 전처리하였을 때, 5 mM GSH 전처리 후 메틸브로마이드 훈증시 약해 저감효과가 두드러졌다. GSH 전처리 시 식물체 내에 MBF1c와 HSP70 유전자 발현이 증가하는 것을 확인하였으며, 이는 메틸브로마이드 훈증으로 유도되는 약해를 방어하는 역할을 담당하였을 것이라고 평가된다. 따라서, 식물검역 훈증제 메틸브로마이드에 의해 발생하는 약해를 저감하는 데 GSH의 사용가능성을 평가하였으며, 이를 기반으로 다양한 식물체에 적용하여 수출입 시 약해로 인한 경제적 손실을 감소시킬 수 있기를 기대한다. Understanding the phytotoxic mechanism of methyl bromide (MB), an essential fumigant during the quarantine and pre-shipment process, is urgently needed to ensure its proper use and reduce international economic losses. In a previous study, two main MB-induced toxic mechanisms such as reactive oxygen species (ROS) and auxin distribution were selected by analyzing transcriptomic analysis. In the study, a 3-week-old A. thaliana was supplied with 1 mM ROS scavengers [N-acetyl-L-cysteine (NAC) or L-glutathione (GSH)] and 1µM indole-3-acetic acid(IAA) three times every 12 h, and visual and gene expression assessments were performed to evaluate the reduction in phytotoxicity by supplements. Phytotoxic effects on the MB-4h exposed group were decreased with GSH application compared to the other single supplements and a combination of supplements at 7 days post fumigation. Among these supplements, GSH at a concentration of 1, 2, and 5mM was suppled to A. thaliana with MB-fumigation. During a long-term observation of 2 weeks after the fumigation, 5 mM GSH application was the most effective in minimizing MB-induced phytotoxic effects with up-regulation of HSP70 expression and increase in main stem length. These results indicated that ROS was a main key factor of MB-induced phytotoxicity and that GSH can be used as a supplement to reduce the phytotoxicity of MB.
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복숭아혹진딧물(Myzus persicae) 약충에 대한 에틸포메이트 훈증 독성의 생화학적 메커니즘
김경남 ( Kyeongnam Kim ),이병호 ( Byung-ho Lee ),박정선 ( Jeong Sun Park ),양정오 ( Jeong Oh Yang ),이성은 ( Sung-eun Lee ) 한국응용생명화학회 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.3
Ethyl formate has been used for the control of insect pests by fumigation. However, there were not many reports to show its target site of fumigant toxicity on insect pests since its first use in the agricultural industry. In the present study, we showed the presumable target sites of ethyl formate fumigation in insect pests using Myzus persicae nymphs. After ethyl formate fumigation, the nymphs of this species were collected and the changes at the biochemical and molecular level were determined. The activity of cytochrome c oxidase (COX) was approximately two-fold higher after ethyl formate fumigation. In addition, the expression levels of acetylcholinesterase (AChE) decreased gradually with increasing ethyl formate concentration. These two findings suggested that COX and AChE might be the major target sites of ethyl formate fumigation. In addition to these results, the analysis of lipid content using MALDI-TOF MS/MS identified 9 phospholipids differently generated 2-fold higher in the ethyl formate-treated nymphs than that in the control nymphs, thereby leading to changes in cell membrane composition in M. persicae nymphs. Therefore, the ethyl formate fumigation caused lethal effects on M. persicae nymphs by changing COX activity, AChE gene expression, and phospholipid production.
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상용차용 Urea-SCR 시스템의 인젝터 냉각방식에 관한 연구
권봉수(Bongsu Kwon),김종훈(Jonghun Kim),김덕진(Deokjin Kim),이천환(Chunhwan Lee),오광철(Kwangchul Oh),이춘범(Chunbeom Lee),김재경(Jaekyeong Kim),김경남(Kyeongnam Kim) 한국자동차공학회 2010 한국자동차공학회 학술대회 및 전시회 Vol.2010 No.11
Urea-SCR system is a high-effective NOx reduction technology in diesel vehicles. Urea injectors typically are installed on the vehicle’s exhaust pipe. While the operating temperature of solenoid-type injector is 50℃, the exhaust gas temperature is higher than the temperature of the injector, and it will cause the overheating problem in the injector. Thus, in order to solve the problem, the cooling jacket (cooling system) is required to maintain the operating temperature of solenoid-type injector. In the paper, it was studied about the cooling performance of injector used in the heavy-duty commercial vehicles. First, the cooling performance by air was evaluated through the engine testing, and ESC and ETC mode are used for engine testing. Second, the cooling performance by water was evaluated through the CFD analysis. Therefore, through this study, we should select a more effective cooling system of the solenoid-type injector
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