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권미향,임은정,성하진 ( Mee Hyang Kweon,En Jung Lim,Ha Chin Sung ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.1
During the screening for anti-complementary activity from 10 kinds of edible mushrooms, an alkali extract of Agaricus bisporus showed the highest activity through the complement fixation test. The crude anti-complementary material(AB-O) from Agaricus bisporus was obtained by the alkali extraction using 1 N NaOH containing 5% urea(65℃), followed by methanol reflux, dialysis and lyophilization. The fraction AB-O showed potent anit-complementary and anti-tumor activity against sarcoma-180 injected mice. The fraction AB-O was divided into 5 fractions(AB-20, AB-40, AB-60, AB-80, AB-A) by gradual acetone precipitation. Among them fraction AB-20 having the highest activity and yield was found to contain 39% carbohydrate and 46% protein. The anti-complementary protein-bound polysaccharide AB-20 consisted of glucose, arabinose, xylose, galactose and mannose in a molar ratio of 6.49 : 1.98 : 1.24 : 1.00 : 0.71, respectively and its main component amino acids were alanine(20.59%), isoleucine(16.85%), glutamine+glutamic acid(14.12%) and leucine (13.83%). The anti-complementary activity of AB-20 was decreased greatly by periodate oxidation, but decreased slightly by pronase digestion. This indicates that polysaccharide moiety is corelated with the anti-complementary activity and that protein is also involved in the activity.
권미향(Mee Hyang Kweon),박미경(Mee Kyung Park),나경수(Kyung Soo Ra),성하진(Ha Chin Sung),양한철(Han Chul Yang) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.2
Screening of anticoagulant activity was conducted for the hot water extracts of 73 kinds of medicinal herbs, 41 kinds of Korean edible plants. and 5 kinds of sea weeds using plasma recalcification test(Tr). In the first screening several extracts of the plants, Alisma calnaliculation, Corydalis ternate. Panax notoginseng. Allium sativum, Ganoderma lucidum, Codium fragile, showed high activities. When the plants were reextracted with various solvent conditions, acidic water extracts of Codium fragile showed the highest activity in APTT. A crude polysaccharide fraction(CF-1) was prepared by methanol reflux, ethanol precipitation, dialysis and lyophilization of the acid extracts. CF-1 comprised 80.8% total sugar consisting of arabinose, galactose and glucose as the main monomers, 8.7% protein, and 13.3% sulfate. The anticoagulant activity of CF-1 was not changed by pronase digestion, but decreased by periodate oxidation, and this indicated that the anticoagulant activity was attributed to the polysaccharide portion.
박미경(Mee Kyung Park),권미향(Mee Hyang Kweon),조홍연(Hong Yon Cho),양한철(Han Chul Yang) 한국응용생명화학회 1999 Applied Biological Chemistry (Appl Biol Chem) Vol.42 No.2
We have isolated two anticoagulant polysaccharides from an acidic extract of Codium fragile. The purification was conducted using three consecutive chromatographies of DEAE-Toyopearl 650C, Sephadex G-100 (G-75), and Sepharose CL-6B by measuring activated partial thromboplastin time (APTT). The two purified anticoagulant polysaccharides, CF-1-VIa-1 and CF-1-VIIa-1, were found to be nearly homogenous on HPLC using a gel permeation column and appeared to have molecular weights of about 80,000 Da and 40,000 Da, respectively. The polysaccharides consisted mainly of arabinose and galactose in a molar ratio of about 2:1, and also comprised 12-13% of sulfates at their constituent sugars. CF-1-VIa-1 and CF-I-VIIa-1 inhibited blood coagulation via both the intrinsic and the extrinsic pathways. The polysaccharides unlike heparin showed an inhibitory activity on thrombin when a pure fibrinogen without antithrombin III was used as a substrate. Structural modifications using sulfation and desulfation affected the anticoagulant activities directly, suggesting that the content of sulfate plays an important role in the blood coagulation cascade. The polysaccharides may inhibit some proteases involved in the blood coagulation cascade, judging from the independence of calcium concentrations in their anticoagulant activity.
아스콜빈산에 의한 Aflatoxin B₁의 파괴에 관한 연구
박건영(Kun-Young Park),권미향(Mee-Hyang Kweon) 한국식품영양과학회 1987 한국식품영양과학회지 Vol.16 No.1
AFB₁과 아스콜빈산(AA)이 산성 PH(PH 1~7)에서 반응하는 동안 (37℃에서 5일간) 상당량의 AFB₁이 파괴되었다. 반응기간 중 AFB₁은 PH5~7 사이에서 대조군의 경우 거의 파괴되지 않았는데 AA가 존재할 때 5일 후 50~66%가 파괴되었다. PH4 이하에서는 대조군 자체에서도 PH가 낮아지면서 AFB₁의 파괴가 증가되었지만 AA 존재하에서는 그 파괴량도 증가되었을 뿐 아니라, PH가 떨어질수록 속도도 증가했다. AFB₁이 AA와 반응했을 때 온도가 증가함에 따라 AFB₁량이 점차 감소하여 60℃에서 반응할 때는 3일 후에 전혀 AFB₁을 발견하지 못했지만 5℃에서는 반응기간 중 AFB₁은 안정했다. AFB₁이 산화제 (CuSO₄ㆍ5H₂O)가 첨가된 AA와 반응했을 때 그 반응속도는 급격히 증가되어 90~96%의 AFB₁이 파괴되었으며, 대조군의 4%만이 파괴된 것과는 대조적이었다. 환원제 L-cysteine을 첨가했을 때 하루동안 AFB₁의 파괴는 거의 없었으며 반응기간 중 대조군보다 소량의 AFB₁이 파괴되었다. AFB₁의 파괴는 AA의 농도에 따라 달랐는데 AA 농도가 감소할수록 파괴 속도가 감소했지만 AFB₁의 농도는 이 파괴 반응에 영향을 주지 못했다. 과 AA가 반응하여 새로이 생성된 반응문은 TLC, RPLC 및 UV spectrophotometer를 이용하였을 때 AFB_(2a)로 동정되었으며, AFB₁의 분해와 더불어 AFB_(2a)의 생성이 확인되었다. 이 실험 결과에 비추어 AA 존재시 AFB₁의 파괴는 AA 산화기간중 생성된 산화 생성물에 의한 것으로 추정된다. Large amount of aflatoxin B₁(AFB₁) is disappeared in the presence of L-ascorbic acid(AA) in buffer solution at pH values from 1 to 7 during 5 days of incubation at 37℃. AFB₁ was quite stable at pH's between 5 and 7 when AA was absent(control), however, 50~60% of AFB₁ was degraded in its presence after 5 days. The rate of disappearance of AFB₁ increased with a decreasing of pH in the presence of AA, even though AFB₁ in the control degraded increasingly with the decrease in pH(pH≤4). The level of AFB₁ decreased as the reaction temperature increased when AFB₁ reacted with AA. The aflatoxin could not be detected at all after 3 days when the reaction occurred at 60℃, while the aflatoxin was stable at 5℃ thoughout the reaction period. 90~96% of AFB₁ was found to be degraded in a day when AFB₁ reacted with AA plus different concentrations of CuSO₄ㆍ5H₂O, showing remarkably faster rate than the control; however, different concentrations of L-cysteine instead of CuSO₄ㆍ5H₂O protected the degradation of aflatoxin and no AFB₁ was degraded for a day and resulted in less AFB₁ disappeared than the control. The degradation of AFB₁ was dependent on AA concentration and the rate of disappearance as the concentration of AA decrease, but AFB₁ concentration did not influence the rate. The product formed when AFB₁ reacted with AA was identified to AFB_(2a) by using HPLC chromatographic examinations, and by UV spectrum of AFB₁ reacted with AA. The disappearance of AFB₁ was correlated well in the appearance of AFB_(2a). From the results, the degradation of AFB₁ in the presence of AA is probably due to one or more of the oxidative products of AA which was produced during the AA oxidation.