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- 저자 : 金鍾冕 (검색결과 273 건)
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生乳에서 分離한 大腸菌群의 藥濟耐性 및 R Plasmids
金鍾冕,宋熹鐘 全北大學校 1978 論文集 Vol.20 No.-
Recent reports confirm that R factors against Enterobacteriaceae isolated from various sources is widespread in Korea. However, few reports have been made concerning the incidence of transferable drug resistance of bacteria contained in milk. A total of 114 strains of coliform bacteria were isolated from raw milk, and classified 32 strains as Escherichia coli, 18 strains as Citrobacter freundii, 64 strains as Klebsiella-Enterobacter group by IMViC system. After that, they were examined for drug resistance and distribution of R plasmids. Among 32 strains of E. coli isolated, 78.1% (25 strains) were resistance to antibacterial agents such as streptomycin(SM), chloramphenicol(CP), gentamicin(GM), ampicillin(AP), kanamycin(KM), tetracycline(TC), and nalidixic acid(NA), singly or in combination. Whereas all strains of Citrobacter freundii and Klebsiella-Enterobacter group were resistant to one or more drugs used. Among 25 resistant strains of E. coli isolated, the rate of multiple resistant strains(60%) was higher than that of singly resistant strains (40%) and 15 different drug resistant patterns were observed. The most frequently encountered patterns were AP, followed by SM and AP KM. Among 18 resistant strains of Citrobacter freundii isolated, the rate of multiple resistant strains(66.7%) was higher than that of singly resistant strains(33.3%) and 5 different drug resistant patterns were observed. Meanwhile, the most frequently encountered patterns were NA, followed by SM CP AP and SM CP AP TC. Among 64 resistant strains of Klebsiella-Enterobacter group isolated, the rate of multiple resistant strains(71.9%) was higher than that of singly resistant strains(28.1%) and 21 different drug resistant patterns were encountered. Among the 18 singly resistant strains, AP pattern (11 strains) was the commonest. While among the 46 multiple resistant strains, SM AP TC, SM AP, SM AP KM TC, and SM CP AP KM TC patterns were frequently observed. Each one strain of E. coli and Citrobacter freundii carried R plasmids, determining AP, and CP TC, respectively. Whereas 8 strains of Klebsiella-Enterobacter group carried R plasmids determining TC, AP, CP, CP TC, CP AT TC, and SM AP TC. The transfer frequency rate of AP in E. coli was 4.2×10 exp (-4)%. In Citrobacter freundii the transfer frequency rate of CP was 7×10 exp (-5)%, and that of TC was 5×10 exp (-5)%. In Klebsiella-Enterobacter group the transfer frequency rate of SM was 2×10 exp (-5)%, that of CP ranged from 1.1×10 exp (-4) to 6.7×10 exp (-4)%, that of AP 2×10 exp (-5) to 1.6×10 exp (-4)%, and that of TC 2×10 exp (-5) to 1.02×10 exp (-3)%.
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病原性葡萄球菌의 同定을 위한 Coagulase, Deoxyribonuclease(DNase) 및 耐熱成 nuclease 生産能의 比較
金鍾冕 全北大學校 生物學硏究所 1981 生物學硏究年報 Vol.2 No.-
A total of 251 clinical isolates (human origin 43 strains and bovine udder origin 208 strains) of the Staphylococcus that fermented mannitol aerobically were tasted for their ability to produce coagulase, deoxyribonuclease(DNase), and thermostable nuclease. Of these, 158 isolates coagulated human or bovine plasma, produced DNase and thermostable nuclease, and were identified as St. aureus, 146 which produced a 1+ to 3+ clot. The remaining twelve isolates produced a ± or - clot with citrate treated plasma but produced 1+ to 3+ clot with ethylenediaminetetraacetic acid(EDTA) treated plasma. It was found that seven coagulase positive isolates failed to produced thermostable nuclease. In these organisms, we found out of the clot formation is not by coagulase activity but utilization of citrate, because EDTA treated plasma is not coagulated. Among 93 isolates which did not coagulate citrate-or EDTA treated plasma and thermostable nuclease negative, 28 strains produced DNase were identified as St. epidermidis, and other strains were not identification further. It was found that thermostable nuclease production appears to be a consistent property of St. aureus and the test is easy to perform, is rapid - became quite within 2 to t hour, and is not influenced by as many factors and variations as the coagulase test.
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金鍾冕,白象基 全北大學校 1968 論文集 Vol.10 No.-
It is generaly accepted that the occourrance of the osteopetrosis is rare in comparison with the other form of the avian leukosis complex. However, Osteopetrosis in fowl in our country is encountered considerablly often. Because the studies on this subject are not yet investigated and reported in our country that we report here on two selected cases of osteopetrosis which we have made hematological and pathological observation. MATERIALS and METHODS Case one is one of an osteopetrotic fowl, Leghorn breed, male, age of 18 months, weighing 1.8kg, selected from poultry farm of Institute of Animal Health in Iri. And the case two is that of mongrel breed, male, age of 20 months,weighing 1.6kg, selected from poultry farm in Chon-ju. Both of fowls were taken X-Ray picture and total counting of erythrocytes and leukocytes and differential counting of leukocytes were practiced. For histopathological observation, the tibial bone was serially sectioned, and decalcified in 5% HCl solution for 14 days and thereafter we made histological preparation for microscopic observation. As for control, we sacrificed two healthy fowl of the same weight and age, treated them as same as the osteopetrotic fowl. RSULTS HEMATOLOGICAL OBSERVATION on total erythrocyte count in case one appeared 1940(thousand) and in case two 910(thousand) respectively which suggest severe anemia due may from obliteration of bone marrow cavity by newly formed bone tissue. Total leukocyte count revealed in case one to by 76(thousand) and in case two to be 200(thousand) which suggest great increase of the leukocytes ranging 10 times as much as the normal. And considering the differential count of the leucocytes which revealed 7.5% of lymphocytes in case one and 79% in case two may well to suggest absolute lymphocytosis. X-RAY FINDING revealed, as shown in Fig., that the tibial bone is greatly thickend bilaterally and marrow cavity is narrowed, otherwise completely ostructed. MACROSCOPICAL OBSERVATION of both cases appeared same, showing irregular thickening of metatarsus, tibia, oss. coracidium, and scapula. In particular, the tibial bone showed so called boot-like appearence. Cut surfaces of tibial bone which were serially sectioned are shown Fig. HISTOPATHOLOGICAL OBSERVATION revealed endosteal and periosteal ossification which could be summarized into four patterns. 1. As shown in Fig., we observed signs of endosteal ossification appearing numerous newly formed bone bar, resembling the trabecular of sponge bone, anastomosing irregularlly one another; osteoblasts appear on the surface of the developing bone in a continuous layer. Between these newly formed bone bar appear fibrous connective tissue with prominant blood vessels. 2. As shown in Fig. , we observed the periosteal ossification revealing multiple layer of osteoblasts developing outerward laterally from the inner part of compact periosteal tissue. 3. As shown in Fig. , we observed the patterns of advanced process of ossification revealing compact interstitial bone tissue in which appear numerous lacunae with burried bone cells. 4. As shown in Fig., we observed the patterns of completely developed compact bone tissue which reveals new bone lamellae which appear condensed, hypercalcified, and devided by relatively thick cement lines. The central portion of osteon like bone tissue reveals cannales. And in the interstitial tissue we observed remnant of fibrous connective tissue and cappilaries in which appear very small number of myeloid elements. CONCLUTION We have observed two cases of osteopetrosis and the results were summarized as follows : 1. We observed absolute lymphocytosis and severe anemia. 2. We observed bilateral thickening of the cortex of the bone by X-Ray and macroscopic observation. And due to endochondral and perichondral ossification, marrow cavities were narrowed, otherwise completely obstructed.
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Toxoplasma gondii의 組機培養에 關한 硏究
金鍾冕 全北大學校 1974 論文集 Vol.16 No.-
Quite a number of diagnostic methods have been studied as to earlier diagnosis, treatment and control of toxoplasmosis where higher rates of occurance and mortality are discerned no less than in cases such as swine cholera and swine erysipelas. Even with the method of dyetest which is universely acknowledged as an efficient serological diagnostic method, however, chances are very slim for the treatment of swine suffering from acute toxoplasmosis because the antibody comes into being around seven days after the symptom. Hence, the major concern of the paper is with the study of the diagnostic method which has proved to be more efficient and valid. The buffy coat cell of three-day cultivation was inoculated with Toxoplasma gondii collected from the circulatory blood of the patients with parasitemia experimentally inoculated right after the symptom. The results obtained therefrom are summarized in the following outline : 1. The buffy coat cell of two-day cultivation was inoculated with buffy layer of the circulatory blood of swine and mouse experimentally infected with Toxoplasma godii. This experiment did not reveal the multiplication which is valuable for the diagnostic method. 2. The buffy coat cell of three-day cultivation was inoculated with buffy layer of the circulatory blood of swine with parasitemia experimentally inoculated. Then started to appear Toxoplsma gondii 24 hours after the inoculation, and the multiplication for valuable diagnosis was observable during the period of 48-72 hours post inoculation. 3. The buffy coat cell of three-day cultivaton was inoculated with buffy layer of the circulatory blood of mouse with parasitemia experimentally inoculated with Toxoplasma gondii, this experiment ending in the same result as in the case of swine.
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