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      • KCI등재
      • 卵丘, 卵管 上皮細胞 및 子宮 內膜細胞와의 共同培養이 돼지 卵胞卵의 體外受精 및 分割率에 미치는 影響에 관한 硏究

        金相根,金明憲,金武剛,李揆丞 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

        The studies were carried out to investigate the effects of co-culture with cumulus cell, oviduct epithelial cells and uterine endometrial cells on the in-vitro fertilization and cleavage rate of porcine follicular oocytes. The ovaries were obtained from slaughtered swine. The follicular oocytes surrounded with cumulus cells were recovered by aspirating follicular fluids from the visible follicles of diameter 3~5mm. The follicular oocytes were cultured in TCM-199 medium containing hormones and 10% FCS for 24~48 hrs in a incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12~18 hrs with motile capacitated sperm by preincubation. The results obtained in these experiments were summarized as follows : 1. The in-vitro maturation and fertilization rate of porcine oocytes co-cultured with mumulus cells in TCM-199 medium were 64.6%~74.5% and 37.5%~55.3%, respectively. And in-viro fertilization rate of cumulus-enclosed oocytes(51.5%) were significantly (p<0.05) higher than cumulus-denuded oocytes(21.7%). 2. The in-vitro maturation and fertilization rate of porcine oocytes co-cultured with 1×10^4 cells/ml,1×10^6 cells/ml, 1×10^8 cells/ml and 1×10^15 cells/ml oviduct epithelial cells in TCM-199 medium were 535% and 37.2%, 617% and 46.8%,54.5% and 31.8%,42.2%and 26.7%, respectively. 3. The in-vitro maturation and fertilization rate of porcine oocytes co-cultured with 1×10^6/ml, 1×10^8/ml, 1×10^15/ml uterine endometrial cells in TCM-199 medium were 54.3% and 39.1%,58.3% and 43.8%, 55.5% and 33.3%, and 45.7% and 30.4%, respectively. 4. When the in-vitro fertilized oocytes were co-cultured with porcine cumulus cells, oviduct epithelial cells and uterine endometrial cells, the development rate to the blastocyst stage was 9.5%, 10.7% and 11.8%, respectively and the rates were higher than that of control, 2.1%(p<0.05).

      • 객체지향 방법론과 구조적 방법론의 비교

        김상근 聖潔大學校 情報産業技術硏究所 1998 情報産業技術論叢 Vol.3 No.-

        Software development is strongly influenced by specific software development methodologies. So, Software developers who want to choose appropriate methodology to problem domain, who have to consider advantages and disadvantages of methodologies. This paper compares a number of analysis and design methodologies. Structured methodologies reviewed include: R/SA, JSD. Object-Oriented methodologies considered include: OOA, OMT. This paper compares specification process, modeling and principles used in methods by Ward and Mellow, Jackson and Cameron, Coad and Yourdon, Rumbaugh.

      • KCI등재

        마테오 리치의 [천주실의]에 나타나 16세기 후반 예수회 대학의 교과과정과 예수회 토미즘(Jesuit Thomism)의 영향

        김상근 한국기독교학회 2005 한국기독교신학논총 Vol.40 No.-

        Matteo Ricci (1552-1610), the founder of the Jesuit mission to Ming China, has been under theological influence from the sixteenth-century Jesuit Thomism and its associated academic curricula. According to John O'Malley's historical analysis, the early Jesuit missionaries' accommodative and flexible mission methods have been resulted from Renaissance humanism's reverence of the classics of the past. However, the author or the present paper further argues that the sixteenth-century Jesuit curricula, which were first shaped by Jerónimo Nadal's De universitate studii generalis (1548) and De Studiis Societatis Iesu (1551), have left direct impacts on the innovative missiontheology of the early Jesuit missionaries, including Matteo Ricci's. The early Jesuits' acceptance of the Spanish Thomism, which had been developed by the Spanish Dominican theologians at University of Salamanca, as their theological framework for the newly-established Jesuit universities, was the major theological background for the establishment of the early Jesuit mission strategies. Matteo Ricci, who wrote T'ien-chu Shih-i, spent four years (1573-1577) at Collegio Romano where the Jesuit Thomism was fully deliberated by Jacob Ledesma (1519-1575), Emanuel Sa (1530-1596), Francisco Toledo (1532-1596), and the famous Cardinal Roberto Bellarmino (1542-1621). The author of the present paper concludes that the influence of the Jesuit Thomism was presented by Matteo Ricci's main argument in T'ien-chu Shih-i, as he claims that Prima Praecepta is preserved in the Chinese's worship of Shangti in the ancient Confucian tradition.

      • 소 體外受精卵의 超急速凍結에 관한 硏究 : Ⅱ. 소 體外受精卵의 超急速凍結 融解後의 生存性에 관한 硏究 Ⅱ. Studies on the Survival Rates after Rapid Frozen-Thawing of In Vitro Fertilized Bovine Embryos

        金相根,李晩徽 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

        This study was carried out in order to investigate the effects of cryoprotective concentration and equilibration time on survival rate of ultrarapidly frozen in vitro fertilized bovine embryos. In vitro fertilized bovine embryos, following dehydration by cryoprotective agents and sucrose were directly plunged into liquid nitrogen and thawed in 38℃ water. Survival rate was defined by development rate to the morula and blastocyst stage after in vitro culture of by FDA test. The results are summarized as follows. 1. The survival rates of in vitro fertilized bovine embryos after ultrarapid frozen-thawing in the freezing medium of 0.25M sucrose added 2.0M, 2.5M. 3.0M, 3.5M, 4.0M glycerol were 75.0%, 72.0%, 67.6%, 44.8% and 18.3% respectively. 2. The survival rates of in vitro fertilized bovine embryos after ultrarapid frozen-thawing in the freezing medium of 0.25M sucrose added 2.0M, 2.5M. 3.0M, 3.5M, 4.0M DMSO were 64.0%, 66.7%, 70.8%, 52.7% and 18.6, respectively. 3. The survival rates of in vitro fertilized bovine embryos after ultrarapid frozen-thawing in the freezing medium of 0.25M sucrose added 2.0M, 2.5M. 3.0M, 3.5M, 4.0M propanediol were 68.4%, 64.9%, 63.2%, 62.2% and 34.7%, respectively. 4. The survival rates of in vitro fertilized bovine embryos after ultrarapid frozen-thawing in the freezing medium of 2.50M glycerol added 0.1M, 0.25M, 0.5M, 0.75M, sucrose were 60.5%, 72.2%, 70.1% and 54.9%, respectively. The survival rates of in vitro fertilized embryos after ultrarapid frozen-thawing in the freezing medium of 2.5M glycerol added 0.25M sucrose were higher than concentration of 0.10M, 0.50M and 0.75M sucrose. 5. The equilibration time on the survival rate of in vitro fertilized bovine embryos was attained after short period of time(2.5∼5min.) in the freezing medium added 0.25M sucrose and 3.0M DMSO higher tham long periodef time(1∼20min.).

      • 한우의 발정(發情) 및 발정전후에 따른 성 호르몬의 수준변화에 관한 연구

        김상근 한국낙농학회 1985 韓國酪農學會誌 Vol.7 No.1

        韓牛의 繁殖過程에 따른 血淸內 性 hormone의 水準變化를 究明하고져 發情週期, 發情前後의 血淸內 FSH, LH, Prolaction, Progesterone 및 estradiol-17β 水準을 radioimmunoassay로 測定하여 比較 檢討하였던 바, 그 結果는 다음과 같다. 1. 發情週期中의 血淸 FSH水準은 全 觀祭時間에서 0.26mIU/㎖이하 水準을 나타냈고, 血淸 LH水準은 發情期가 가까와질수록 增加하여 發情후 20∼22日에는 2.02±0.40mIU/㎖로서 最高値를 나타냈으며, 血淸 prolactin水準은 3.72±1.27ng/㎖∼5.62±1.64ng/㎖로서 큰 變化를 나타냈으며, 發情直前에 높은 水準을 維持하였다. 2. 發情週期中의 血淸 progesterone 水準은 黃體期인 14∼16日에 6.72±1.82 ng/㎖∼8.24±1.76ng/㎖로서 最高値를 나타냈으며, 血淸 estradiol-17β 水準은 3.62±1.10pg/㎖∼ 12.78±1.82pg/㎖로서 큰 變化를 나타냈고, 發情當日에 最高値를 나타냈다. 3. 發情前後의 血淸 FSH水準은 全 觀察時間에서 0.26mIU/㎖以下水準을 나타냈고, 血淸 LH 및 prolactin水準은 發情當日에 各各 2.78±0.69mIU/㎖와 5.72±0.92㎎/㎖로서 最高値를 나타냈다. 4. 發情前後의 血淸 progesterone水準은 發情日에는 2.75±0.78㎎/㎖로서 最高値를 나타냈으며, 血淸 estradiol-17β水準은 發情日에 13.21±1.42pg/㎖로서 最高値를 나타냈으며 發情 24時間前後는 減少된 水準이었다. The study was carried out to find out the changes of the sex hormone concentrations in the serum of Korean native cows during the estrous cycle, and before and after estrus. The FSH, LH, progesterone, estradiol-17β and prolactin concentrations from the serum were assayed by radioimmunoassay. The results of this study were summarized as follows: 1. The FSH concentrations during the estrous cycles were less than 0.26 mIU/㎖. The LH concentrations during the estrous cycles were showed a peak level of 2.02±0.4 mIU/㎖ and the prolactin concentrations ranged from 3.72±1.27 ng/㎖ to 5.62±1.64 ng/㎖ showing significant changes and maintained high levels before estrus. 2. The progesterone concentrations during the estrous cycle ranged from 6.72±1.82 ng/㎖ to 8.24±1.76 ng/㎖ at luteal phase. The estradiol-17β concentrations during the estrous cycles ranged from 3.62±1.10 pg/㎖ to 12.78±1.82 pg/㎖ showing significant changes and showed a peak level at estrus. 3. The FSH concentrations before and after estrus were less than 0.26 mIU/㎖. The LH and prolactin concentrations before and after estrus were reached a peak level of 2.78±0.69 mIU/㎖ and 5.72±0.92 ng/㎖ respectively. 4. The progesterone concentrations before and after estrus were decreased a level of 2.75±0.78 ng/㎖ at estrus. The estradiol-17β a concentrations showed a peak level of 13.21±1.42 pg/㎖ and decreased at 24 hrs. before and after estrus.

      • 개신교의 교육제도 및 현황

        김상근 중앙승가대학교 1994 僧伽 Vol.11 No.-

        올바른 신앙은 결코 교회라는 울타리 안에 머물지 않는다. 하나님이 하늘을 버리고 인간세계에 오신 것이 바로 이러한 신앙의 표상이다. 물론 여기서 하늘은 상징적 언어요 표상이다. 공간적인 개념으로 이해한다면 그 옹골진 의미를 파악해낼 수 없게 된다. 사실 우리나라의 여러 제도종교는 이 같은 성격을 모두 가지고 있다. 고려․조선 시대의 호국과 조선왕조 해체기의 척화양이 제폭구민과 일제하 독립운동 그리고 최근의 인권․민주화․민족의 화해와 통일운동이 이 나라의 바른 종교가 쌓는 전통이다. 이제 우리는 교리 논쟁을 떠나 이 소중한 전통을 이어받을 수 있어야 할 것이다. 이에 부응하는 교육과 자기 개발을 기대해 마지않는다.

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